Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,804,531 G→T 11.7% intergenic (+70/‑284) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,804,5310GT11.7% 176.7 / 7.9 77intergenic (+70/‑284)nrdF/proVribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (40/25);  new base T (2/8);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATT  >  NC_000913/2804382‑2804680
                                                                                                                                                     |                                                                                                                                                        
aCGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACg                                                                                                                                                          >  1:1109997/1‑150 (MQ=255)
aCGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACg                                                                                                                                                          <  1:319180/150‑1 (MQ=255)
 cGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGCAAATAATTCCCGCCAAATAGCTTTTTATCACGc                                                                                                                                                         >  2:1050816/1‑150 (MQ=255)
          ttCCGGTTCAGCCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGATATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCCCAAAATAGCTTTTTATCACGCAAATAAttt                                                                                                                                             >  1:393723/1‑150 (MQ=255)
            ccGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAATTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTtgt                                                                                                                                           <  1:925745/150‑1 (MQ=255)
              ggTTCAGGCTCCTCTTATGTGATGTGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCATCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtgg                                                                                                                                         <  1:517871/150‑1 (MQ=255)
               gTTCAGGCTCCTCTTATGTGATGGTGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggt                                                                                                                                        <  2:96268/150‑1 (MQ=255)
                ttCATGCTCCTCTTATGTGATGGGGAAAGCGTTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGTAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                       <  2:868912/150‑1 (MQ=255)
                ttCAGGCTCCTCTTTTGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                       <  2:1286708/150‑1 (MQ=255)
                tgaaGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTgtggtg                                                                                                                                       <  2:192638/147‑1 (MQ=255)
                 tCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGAAGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCACCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGa                                                                                                                                      <  2:611018/150‑1 (MQ=255)
                  cAGGCGCCTCATCTGTGATGGCGCAAGCGGTTGAAACAGAAGTTGTAGACTGGTATTTCTGAGGGTTTTATTTTCAAAAATTTCACTACCCGCAGCAGGGAAATAATTCCCGCCACATAGCTTTTTATCACGCAAATAATTTGTGGTGAt                                                                                                                                     <  2:130968/150‑1 (MQ=255)
                         ctctTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACt                                                                                                                              >  2:1045271/1‑150 (MQ=255)
                             tATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATAACGCAAATAATTTGTGGTGATCTACACTGATa                                                                                                                          >  1:286656/1‑150 (MQ=255)
                              aTGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAc                                                                                                                         <  1:1040865/150‑1 (MQ=255)
                                gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATActc                                                                                                                       <  2:701776/150‑1 (MQ=255)
                                gtgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTCATActc                                                                                                                       >  2:807418/1‑150 (MQ=255)
                                 tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATATGTGGTGATCTACACTGATActct                                                                                                                      >  1:177180/1‑150 (MQ=255)
                                 tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTAACACCCAAATAATTTGTGGTCATAAACACTGATActct                                                                                                                      >  1:425457/1‑150 (MQ=255)
                                 tgATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCAGCAGCAGGGAAATAATTACAGCCAAATAGCTTTTTATCACGCAAATAATTTGTGATGATCTACACTTATAatct                                                                                                                      >  1:934669/1‑150 (MQ=255)
                                    tGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGtt                                                                                                                   >  1:83205/1‑150 (MQ=255)
                                         aaatctgTTGAAACAGAACAAGACCCCTCGCAATTCTGAGGGTGTTATTTTCAAAAATAGCACTACCCGCATCAGGGAAATAATTCCCTCCAAATATTTTTTTTTATGTTAATTATATTTTGGAGATCTACACTGATACTCTGTTGCatt                                                                                                              <  2:1172293/144‑1 (MQ=255)
                                          aaGCGGTTGAAACAGAAGATGAAGACTGTAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGAGCTACACTGATACTCTGTTGCatta                                                                                                             <  1:967729/150‑1 (MQ=255)
                                          aaGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCatta                                                                                                             <  1:1239576/150‑1 (MQ=255)
                                          aaGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCatta                                                                                                             <  1:541714/150‑1 (MQ=255)
                                                ttGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGcc                                                                                                       <  1:341043/150‑1 (MQ=255)
                                                ttGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGcc                                                                                                       <  1:1302078/150‑1 (MQ=255)
                                                  gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTg                                                                                                     >  1:394642/1‑150 (MQ=255)
                                                  gAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGAATTATTCGCCTg                                                                                                     >  1:909476/1‑150 (MQ=255)
                                                      cAGAAGATGAAGACTGTAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCACGCTAATTTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAc                                                                                                 <  2:1101734/150‑1 (MQ=255)
                                                      cAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCAGTAAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAAc                                                                                                 <  2:1109849/150‑1 (MQ=255)
                                                        gaagaTGAAGACTGGAATGTCTGAGGGTGTTATTTTCATAAATAGCACTACCCGCAGCAGGCAAATAATTCCCGCCAATTAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTTCATTATTCGCCTGAAAccc                                                                                               >  1:77043/1‑149 (MQ=255)
                                                          agaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACcaca                                                                                             >  1:1241814/1‑150 (MQ=255)
                                                           gaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCATGGAAATAATTCCAGACAATTTTTTTTTTTTATGTTAATTATATTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACaa                                                                                            <  2:1050929/150‑1 (MQ=255)
                                                           gaTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACcaca                                                                                             >  2:782765/1‑150 (MQ=255)
                                                            aTGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAat                                                                                           >  2:802562/1‑150 (MQ=255)
                                                             tGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACTCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAata                                                                                          >  2:539220/1‑150 (MQ=255)
                                                                 gACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAAATCCCGCCAAATATCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGAATTATTCTCCTGAAACCACAATAATCa                                                                                      >  2:1179506/1‑150 (MQ=255)
                                                                     ggAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATAACGCAAATAATTTGTTGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCg                                                                                  >  2:479054/1‑150 (MQ=255)
                                                                       aaTTTCTGAGGGGGGTATATTCAAAAATATCACTACCCGCAGCAGGTAATTAATAGCAGACTTTTATTTTTTTTTATGTTAATTATTTTTTCGTGATCTAAACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGtg                                                                                <  2:648418/150‑2 (MQ=255)
                                                                             tGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGACTGAAACCACAATATTCAGGCGTTTTTTCg                                                                          >  1:779728/1‑150 (MQ=255)
                                                                               aGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCAGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTAGCt                                                                        >  2:266610/1‑150 (MQ=255)
                                                                                    gtTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCtt                                                                   <  1:1096717/150‑1 (MQ=255)
                                                                                     ttATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAATCCACAATATTCAGGCGTTTTTTCGCTATCttt                                                                  >  1:1281463/1‑150 (MQ=255)
                                                                                      tATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTg                                                                 >  1:33796/1‑150 (MQ=255)
                                                                                       aTTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGATTTTTATATGGTAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGa                                                                <  2:1235039/150‑1 (MQ=255)
                                                                                        ttttCAAAAATATCACTACCCGCAGCAGGGAAATATTTCCCGACAAATTTTTTTTTTTATGTTAATGATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGAc                                                               <  2:729664/150‑1 (MQ=255)
                                                                                        ttttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGAc                                                               <  2:291284/150‑1 (MQ=255)
                                                                                         tttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACa                                                              >  2:906960/1‑150 (MQ=255)
                                                                                          ttCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaa                                                             >  1:544542/1‑150 (MQ=255)
                                                                                           tCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaa                                                            <  1:1334450/150‑1 (MQ=255)
                                                                                            cAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                           >  2:1339724/1‑150 (MQ=255)
                                                                                            cAAAAATATCACTACCCGCAGCAGGGAAATAATTACCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaa                                                           >  2:251865/1‑150 (MQ=255)
                                                                                              aaaaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACaaaaaa                                                         >  2:550290/1‑150 (MQ=255)
                                                                                                aaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTTATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTATCGCTATCTTTTACAAAAAata                                                       >  2:99611/1‑150 (MQ=255)
                                                                                                aaTATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATTTTTGACAAAAAata                                                       >  1:35669/1‑150 (MQ=255)
                                                                                                  tatCACTACCCGCAGCAGGGAAATAATTACCGACAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATc                                                     <  2:286656/150‑1 (MQ=255)
                                                                                                   atCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCa                                                    >  2:909484/1‑150 (MQ=255)
                                                                                                   atCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTAACACGCAAATAATTTGTCGTGATCTACACTGAAACTCTGTTGCATTATTCGCCTGAAACCACAAAATTCACGCGTTTTTTCGCTAACATTGACAAAAAATATCa                                                    >  2:579704/1‑150 (MQ=255)
                                                                                                      aCTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGAGTTTTTTCGCTATCTTTGACAAAAAATATCAACt                                                 >  2:1265005/1‑150 (MQ=255)
                                                                                                       caaCCCGCATCAGTGAAATAATTCCCGCCAAATTTTTTTTTTTATGTTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACtt                                                <  2:792394/148‑1 (MQ=255)
                                                                                                          cccGCAGCAGGGAAATAATTCCCTCCAAATATATTTTTATCACCCAAATAATTTGTGGTGATCTACACTGATACTCTGTTTCATTATTCGACTGAAACCACAAAATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtct                                             >  2:77867/1‑150 (MQ=255)
                                                                                                           ccGCAGCAGGGAAATAATTCCCGCCAAATAGATTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctc                                            <  2:1109997/150‑1 (MQ=255)
                                                                                                                 cagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctc                                            <  1:118849/144‑1 (MQ=255)
                                                                                                                 cagGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTtctc                                            >  2:118849/1‑144 (MQ=255)
                                                                                                                     gAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATAATCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTAGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGatct                                  >  2:1260871/1‑150 (MQ=255)
                                                                                                                      aaaTAATTCCCGCCAAATATCTTTTTATCACTAAATTAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCtctc                                 <  2:1241814/150‑1 (MQ=255)
                                                                                                                           atTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGccc                            >  2:594970/1‑150 (MQ=255)
                                                                                                                            ttCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTTATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCt                           <  1:1350262/150‑1 (MQ=255)
                                                                                                                              cccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTta                         >  1:285403/1‑150 (MQ=255)
                                                                                                                              cccGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTta                         >  1:1255337/1‑150 (MQ=255)
                                                                                                                                  ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATc                     >  2:375615/1‑150 (MQ=255)
                                                                                                                                  ccAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACAATGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTAAGTCGTTTTTACCCTATCTTTGACAAAAAATATAAACTTTATCGATTTAATCTCAACAATTATATc                     >  2:433088/1‑150 (MQ=255)
                                                                                                                                      aTAGATTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACgg                 <  2:1063675/150‑1 (MQ=255)
                                                                                                                                           tttttATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGACACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGAGCTCAGCCCTTATATCACGGGAAAt            >  2:207010/1‑150 (MQ=255)
                                                                                                                                            ttttATATCGTAATTATTTTTTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGTCCTTATATCACGGGAAAtt           <  2:779728/150‑1 (MQ=255)
                                                                                                                                                aTCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCgg       >  2:806792/1‑150 (MQ=255)
                                                                                                                                                  ctcGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCg     >  2:514324/3‑150 (MQ=255)
                                                                                                                                                    cGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGATCTCATCCCATATATAACGGGAAATTCCGGCGAt   >  2:875504/1‑150 (MQ=255)
                                                                                                                                                     gCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAtt  >  1:296249/1‑150 (MQ=255)
                                                                                                                                                     gCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAtt  <  2:909476/150‑1 (MQ=255)
                                                                                                                                                     gCAAATAAATTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGAtt  >  1:1171624/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
ACGATTTCTTTTCCGGTTCAGGCTCCTCTTATGTGATGGGGAAAGCGGTTGAAACAGAAGATGAAGACTGGAATTTCTGAGGGTGTTATTTTCAAAAATATCACTACCCGCAGCAGGGAAATAATTCCCGCCAAATAGCTTTTTATCACGCAAATAATTTGTGGTGATCTACACTGATACTCTGTTGCATTATTCGCCTGAAACCACAATATTCAGGCGTTTTTTCGCTATCTTTGACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATT  >  NC_000913/2804382‑2804680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: