Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,913,039 A→G 6.1% Y205H (TAC→CAC)  relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,913,0390AG 6.1% 245.2 / 5.0 82Y205H (TAC→CAC) relA(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (36/40);  new base G (2/3);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAAT  >  NC_000913/2912892‑2913183
                                                                                                                                                   |                                                                                                                                                
tCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAAc                                                                                                                                                <  1:884247/150‑1 (MQ=255)
tCGGACGACCATACACTTCCGATTTAACGCCTTCAGGTTTCATCTAAGCGCGCAGATGACCAAATAACTCTTCGATGTAGTGTTCGCGGTCGTGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTTTATTCGGTTGGTTGGAGGTatc                                                                                                                                                <  2:429417/150‑3 (MQ=255)
  ggACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACgg                                                                                                                                              <  2:640332/150‑1 (MQ=255)
  ggACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACgg                                                                                                                                              >  2:1093619/1‑150 (MQ=255)
   gacgacCATACACTTCCGCTTTAACGCCTTCAGATTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGTTGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGa                                                                                                                                             <  2:79991/150‑1 (MQ=255)
      gacCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGc                                                                                                                                          <  1:508845/150‑1 (MQ=255)
         cATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGCCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGt                                                                                                                                       >  2:1189815/1‑150 (MQ=255)
              acTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCt                                                                                                                                  <  2:878278/150‑1 (MQ=255)
                  ccGCTTTAACGCCTTCATCTTTCATCTCCGCGCGCAGATGACCACCGCACTCTTCGATGTAGGGTTCGCGGGCGAGACGCCGTTCATGCAGCAGTTTGGCAATCCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCa                                                                                                                              <  2:896627/150‑1 (MQ=255)
                     cTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCTAGtt                                                                                                                           <  1:497662/150‑1 (MQ=255)
                      tttAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGTAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTc                                                                                                                          <  1:1263102/150‑1 (MQ=255)
                        tAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTccc                                                                                                                        >  1:288218/1‑150 (MQ=255)
                             ccTTCAGCTTTCATCTCATCGCGCAGATGACAAAGTAACTCTTCGCTGTAGTGATCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTc                                                                                                                   <  2:566441/150‑1 (MQ=255)
                                tCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGt                                                                                                                <  1:699187/150‑1 (MQ=255)
                                tCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGt                                                                                                                <  1:712950/150‑1 (MQ=255)
                                tCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGt                                                                                                                <  1:796569/150‑1 (MQ=255)
                                  aGCTTTCATCTCAGCGCGCAGCTGACCAACGAACTCTTGGATGTAGTGTTCGCGGTCGAGCCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTg                                                                                                              <  1:659173/150‑1 (MQ=255)
                                    cTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTTGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTc                                                                                                            <  1:1044032/150‑1 (MQ=255)
                                    cTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACTGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTc                                                                                                            >  1:647474/1‑150 (MQ=255)
                                    cTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTc                                                                                                            >  1:417601/1‑150 (MQ=255)
                                     tttCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTcc                                                                                                           >  2:585300/1‑150 (MQ=255)
                                      ttCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCg                                                                                                          <  1:984134/150‑1 (MQ=255)
                                        cATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGCCGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTGACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAt                                                                                                        >  2:639054/1‑150 (MQ=255)
                                        cATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTGACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGAt                                                                                                        >  2:1069420/1‑150 (MQ=255)
                                         aTCTCAGCGCGCAGATGACCAACGAACTCGTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGATGTCCGAtt                                                                                                       >  2:1286258/1‑150 (MQ=255)
                                          tctcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGTAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTc                                                                                                      <  1:1005669/150‑1 (MQ=255)
                                          tctcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTc                                                                                                      >  1:375265/1‑150 (MQ=255)
                                            tgaGCGCGCAGATGACGAAAGAACTCTTAGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGGTTGTCAATTCGTTTGTATTCGGTTGGATGGAGGTATCGGATGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCg                                                                                                    <  1:570579/148‑1 (MQ=255)
                                            tcAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCg                                                                                                    <  1:995032/150‑1 (MQ=255)
                                             cAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCga                                                                                                   >  2:942086/1‑150 (MQ=255)
                                                cgcgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGAGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGAc                                                                                                <  1:1302920/150‑1 (MQ=255)
                                                  cgcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACgg                                                                                              >  1:1110958/1‑150 (MQ=255)
                                                   gcAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTGGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGt                                                                                             <  1:1171546/150‑1 (MQ=255)
                                                    cAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGtt                                                                                            >  1:1215381/1‑150 (MQ=255)
                                                        tGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGcc                                                                                        >  2:1146412/1‑150 (MQ=255)
                                                        tGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAAGTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGcc                                                                                        >  2:1031898/1‑150 (MQ=255)
                                                        tGACCAACGAACTATTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGTAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGcc                                                                                        <  1:823888/150‑1 (MQ=255)
                                                           ccAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGc                                                                                     <  1:612599/150‑1 (MQ=255)
                                                            cAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCg                                                                                    >  2:1108176/1‑150 (MQ=255)
                                                               cGAACTCTTCGATGTAGTGTTCGCTGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTg                                                                                 <  1:801842/150‑1 (MQ=255)
                                                                 aaCTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCg                                                                               <  2:1300918/150‑1 (MQ=255)
                                                                  aCTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGt                                                                              >  2:587712/1‑150 (MQ=255)
                                                                   ctctTCGATGTAGTGTTCGCGGTAGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCAAGCGGTGCGTa                                                                             >  1:1112122/1‑150 (MQ=255)
                                                                     ctTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGa                                                                           >  2:1070996/1‑150 (MQ=255)
                                                                       tCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATg                                                                         <  1:2678/150‑1 (MQ=255)
                                                                       tCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATg                                                                         <  2:899253/150‑1 (MQ=255)
                                                                        cGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACTGAAGCAGTAATCTTCCAGTTCCCATTTCAGATGTCCGATTCCGAGACGGTTAGCCAGCGGAGCCTAGaact                                                                        >  2:131875/1‑147 (MQ=255)
                                                                         gATGTAGGGTTCGCGGTCGAGCCGCCGTTCATGCAGCAGTATGGCAATTCGATTGTATTCGGTTGGATGCAGGTCACGGACGCAGTTTTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGtt                                                                       <  1:235279/150‑1 (MQ=255)
                                                                          aTGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCTAGACGGTTAGCCAGCGGTGCGTAGATGTTg                                                                      >  2:460582/1‑150 (MQ=255)
                                                                            gTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGt                                                                    <  2:271587/150‑1 (MQ=255)
                                                                             tAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTa                                                                   <  1:398277/150‑1 (MQ=255)
                                                                             tAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTa                                                                   >  2:238656/1‑150 (MQ=255)
                                                                             tAGTGTTCGCGGTCGAGACGCCGATCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGCTTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTa                                                                   >  1:129138/1‑150 (MQ=255)
                                                                              tttgtTCGCGGGCGAGACGCCGTTTATTCTGCAGTTTGGCAATTGGTTTGGATTCGGTGGGTCGGCGGTACCGGCACATGTAATCTTCCAGTACCCATTTCAGTTGTGCGATACGGAGACGGTTAGCCAGCGGTTCGTAGATGTTGGTac                                                                  <  1:174532/148‑1 (MQ=255)
                                                                                  ttCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACActc                                                              <  2:1282538/150‑1 (MQ=255)
                                                                                  ttCGCGGTCGAGACGCCGGTCATGCAGCAGTTTGGCAATTCGGTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTATTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACActc                                                              <  2:738998/150‑1 (MQ=255)
                                                                                      cgGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCtttt                                                          <  2:417601/150‑1 (MQ=255)
                                                                                                  cGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTacc                                              >  2:1057915/1‑149 (MQ=255)
                                                                                                  cGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTACCATATCAGTTGTCAGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTacc                                              >  1:188748/1‑149 (MQ=255)
                                                                                                    gtCATGCCGCAGTTTGGCAATTCGGTTGTATGCGGTTGGAGGGAGGTACCGTAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGCGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACg                                            <  2:1291779/149‑1 (MQ=255)
                                                                                                     tCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTGACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGt                                           <  1:639054/150‑1 (MQ=255)
                                                                                                     tCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTGACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGt                                           <  1:1069420/150‑1 (MQ=255)
                                                                                                     tCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGt                                           <  1:585300/150‑1 (MQ=255)
                                                                                                     tCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGt                                           <  1:1070996/150‑1 (MQ=255)
                                                                                                     tCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGt                                           <  1:429652/150‑1 (MQ=255)
                                                                                                      cATGCAGCAGATTGGCAATTCGTTTGTATTCGGTTGGATGTAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGtt                                          >  2:13007/1‑150 (MQ=255)
                                                                                                      cATCCAGCAGATTGCCAATTCGTTTGTATTCGGTTGGATGTAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCCGTACACGtt                                          >  2:17948/1‑150 (MQ=255)
                                                                                                          cagcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGACAGCGGTTCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATc                                      >  1:38935/1‑150 (MQ=255)
                                                                                                            gcagTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCtt                                    >  2:162116/1‑150 (MQ=255)
                                                                                                               gTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCg                                 <  1:942086/150‑1 (MQ=255)
                                                                                                                  tGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGgcg                              >  1:1134706/1‑150 (MQ=255)
                                                                                                                   ggCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGgcgc                             >  2:1187699/1‑150 (MQ=255)
                                                                                                                           gTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCACATCTTTTa                     <  1:691538/150‑1 (MQ=255)
                                                                                                                              tGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACtt                  >  2:194238/1‑150 (MQ=255)
                                                                                                                                tATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTcg                >  2:358153/1‑150 (MQ=255)
                                                                                                                                      gTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGa          >  2:501036/1‑150 (MQ=255)
                                                                                                                                       ttGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTAAACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGAt         >  2:941842/1‑150 (MQ=255)
                                                                                                                                        tggatggaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATg        <  1:1189815/150‑1 (MQ=255)
                                                                                                                                        tggatggaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATg        >  2:274401/1‑150 (MQ=255)
                                                                                                                                         ggatggaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGa       >  1:1346388/1‑150 (MQ=255)
                                                                                                                                           atggaGGTGACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGc     <  2:911991/150‑1 (MQ=255)
                                                                                                                                             ggaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTGGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCaa   <  2:952082/150‑1 (MQ=255)
                                                                                                                                              gaGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAAt  >  2:404473/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGTTGGATGGAGGTAACGGAAGCAGTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAAT  >  NC_000913/2912892‑2913183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: