Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,973 T→C 5.8% A222A (GCT→GCC)  ygdH → UPF0717 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,9730TC 5.8% 206.7 / 2.4 70A222A (GCT→GCC) ygdHUPF0717 family protein
Reads supporting (aligned to +/- strand):  ref base T (33/32);  new base C (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAA  >  NC_000913/2926830‑2927118
                                                                                                                                               |                                                                                                                                                 
ggCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCg                                                                                                                                             >  1:1214608/1‑150 (MQ=255)
  ccTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTTAACCGcc                                                                                                                                           >  2:1236350/1‑150 (MQ=255)
  ccTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGcc                                                                                                                                           <  2:561809/150‑1 (MQ=255)
       gTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAAcc                                                                                                                                      <  2:868099/150‑1 (MQ=255)
             tGAATATCTGCACCGGCTGTGGTCCGGGAGCGTTGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTgg                                                                                                                                <  1:795100/150‑1 (MQ=255)
             tGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTgg                                                                                                                                >  1:1307395/1‑150 (MQ=255)
              gAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCg                                                                                                                                    >  2:182417/1‑145 (MQ=255)
              gAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCg                                                                                                                                    <  1:182417/145‑1 (MQ=255)
                   tCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACg                                                                                                                          >  1:947683/1‑150 (MQ=255)
                   tCTGCACCGGCTGTGGGCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACg                                                                                                                          <  1:122775/150‑1 (MQ=255)
                       cACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAAtt                                                                                                                      <  1:707232/150‑1 (MQ=255)
                         ccGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGa                                                                                                                    <  2:1180920/150‑1 (MQ=255)
                         ccGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGa                                                                                                                    >  2:1037894/1‑150 (MQ=255)
                         ccGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCAGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGa                                                                                                                    >  2:408802/1‑150 (MQ=255)
                           ggCTGTGGGCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACATGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGGCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGatc                                                                                                                  <  2:838100/150‑1 (MQ=255)
                              tgtgGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGATCCGTCGATTATAGCCGCATAACCGCCAAACCCGCAGGTCAACGAATTGatcata                                                                                                               >  2:123246/1‑149 (MQ=255)
                               gtgGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGAtcatca                                                                                                              <  2:1332577/150‑1 (MQ=255)
                                 gggccGGGAGCGATGGAATCGCCGATGAAAGGGGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATg                                                                                                            <  2:230190/147‑1 (MQ=255)
                                 ggTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGCCACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGCTCCTCATg                                                                                                            >  1:1142178/1‑150 (MQ=255)
                                 ggTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGCCAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATg                                                                                                            >  1:31581/1‑150 (MQ=255)
                                 ggTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGGCAACGAATTGATCATCATg                                                                                                            <  2:233718/150‑1 (MQ=255)
                                  gTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAAc                                                                                                                                       <  2:932582/122‑1 (MQ=255)
                                  gTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAAc                                                                                                                                       >  1:932582/1‑122 (MQ=255)
                                   tCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGcc                                                                                                          <  1:939199/150‑1 (MQ=255)
                                          gCGATGGAAGCGCCGATGAACGGGGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGTCAGTGGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATc                                                                                                   <  2:851736/150‑1 (MQ=255)
                                          gCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATc                                                                                                   <  2:1105788/150‑1 (MQ=255)
                                           cGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCg                                                                                                  >  2:617004/1‑150 (MQ=255)
                                             aTGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGaa                                                                                                >  2:479562/1‑150 (MQ=255)
                                               ggAAGCGCCGATGCAAGGTGCTGAGGTCGGACACGCGCAGCAGCGTTAGAAACACAGGCGTGTTATTGGTTTGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAAGTGATCATCATTCAAGAGATCGaaaa                                                                                              >  2:60574/1‑150 (MQ=255)
                                               ggAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGaaaa                                                                                              >  1:315922/1‑150 (MQ=255)
                                               ggAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCCGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGaaaa                                                                                              >  2:342643/1‑150 (MQ=255)
                                               ggAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCCGAACCGCCTAACCCGCTGGTCAACGAATTGATAATCATGCCAGATATCGaaaa                                                                                              >  2:347550/1‑150 (MQ=255)
                                                gAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGaaaaa                                                                                             >  2:15311/1‑150 (MQ=255)
                                                        gATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGa                                                                                     >  1:1145008/1‑150 (MQ=255)
                                                          tGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAg                                                                                   <  1:841023/150‑1 (MQ=255)
                                                            aaaGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCg                                                                                 >  1:1090391/1‑150 (MQ=255)
                                                               ggTGCTGCGGTCGGACACGCGCAGCAGCGTTACCAAGACAGTCGTTTTATTGGTATGACAGAGCCGGCGATTATCGCCGCTGAACCGCCGAACCCGCGGGTCCACGCCGTGCGCATCTTGCGAGATATCGAAAAACGTCGGGTAGCGgtt                                                                              >  1:547780/1‑150 (MQ=255)
                                                                gTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTg                                                                             <  2:566719/150‑1 (MQ=255)
                                                                  gctgctTTCTGACACGCTCAGAAGCGTTACAAAGACAGTCGTTTTTTTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCTTGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTc                                                                           <  2:767920/150‑1 (MQ=255)
                                                                       ggTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTTTTGGTATGACTGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGGCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTAt                                                                      <  2:1077288/150‑1 (MQ=255)
                                                                            gACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTc                                                                 <  2:268055/150‑1 (MQ=255)
                                                                            gACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTc                                                                 >  2:1121372/1‑150 (MQ=255)
                                                                            gACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTc                                                                 <  2:1150667/150‑1 (MQ=255)
                                                                                cgcgCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACgg                                                             >  1:1132475/1‑150 (MQ=255)
                                                                                cgcgCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACgg                                                             >  1:919945/1‑150 (MQ=255)
                                                                                cgcgCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGACGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAATCGTTTGTCCGTATCGCTCACgg                                                             <  1:1202809/150‑1 (MQ=255)
                                                                                    aagcatcGTTACAAAGACAGTCGATTTATTGGTATGACAGAGCCGGCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATc                                                         <  2:259028/149‑1 (MQ=255)
                                                                                           gTTACAAAGCCAGTCGTTTTATTGGTATGACAGCGCCGTCGATTATCGCCGCTGAACCGCCTAAACAGCTGTGCAACGAATTTAGCATCATGCAAGATAGCGAAAAATGTCTGGAAGCGTTTGTCCGTATCGCTCACGTGATCATTAtat                                                  >  1:178840/1‑148 (MQ=255)
                                                                                             tACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTc                                                <  1:337849/150‑1 (MQ=255)
                                                                                                  aGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCAGCTTGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTgg                                           <  1:77871/150‑1 (MQ=255)
                                                                                                   gACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCATATATCGAAAAAAGTCTGGAAGCGTTTGTCCGTATCGATCACGGTATCATTATCTTCCCTGGc                                          >  1:714709/1‑150 (MQ=255)
                                                                                                   gACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGc                                          <  2:947683/150‑1 (MQ=255)
                                                                                                   gACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGc                                          <  1:811591/150‑1 (MQ=255)
                                                                                                             ttATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTa                                <  2:974261/150‑1 (MQ=255)
                                                                                                              tATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTAc                               >  1:649923/1‑150 (MQ=255)
                                                                                                               aTTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACg                              <  2:397895/150‑1 (MQ=255)
                                                                                                                 tGGTATGACAGAGCCGTCGATTATCGCCGCCGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGc                            <  1:347550/150‑1 (MQ=255)
                                                                                                                 tGGTATGACAGAGCCGTCGATTATCGCCGCCGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGc                            <  1:342643/150‑1 (MQ=255)
                                                                                                                  ggTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCa                           >  1:774457/1‑150 (MQ=255)
                                                                                                                   gTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTCACCCGCTGGTCAACGAATTGAGCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATAGTCACATCCGGGGTGGGTACGACag                          >  1:829310/1‑150 (MQ=255)
                                                                                                                    tATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCaga                         >  2:365631/1‑150 (MQ=255)
                                                                                                                       gACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCagaaga                      <  2:774457/150‑1 (MQ=255)
                                                                                                                       gACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACACGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGCAAACGTTTGTCAGAATCGCACACGGTATCATTAACTACCATGGCGGTGTGGGGACGGCagaagc                      >  1:38884/1‑149 (MQ=255)
                                                                                                                           gagCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTTGATGCGTTTGTCCGTATCGCTCAAGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTg                  >  2:85396/1‑150 (MQ=255)
                                                                                                                           gagCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCTCTAACGGTATCATTTTCTTCCATGGCGGTGTGGGTAAGGCATAAGAGATg                  >  2:1165976/1‑150 (MQ=255)
                                                                                                                            tgCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGc                 <  1:1236350/149‑1 (MQ=255)
                                                                                                                             gCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCATCGAATTGATCATGATGCCAGATATCGATAAACGTCTGGAAGCGTTTGTCCGTTTCGCTCTCGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGct                >  1:865435/1‑150 (MQ=255)
                                                                                                                                          gccgcTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGa   >  1:227098/1‑150 (MQ=255)
                                                                                                                                           ccgcTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTAACGGTATCATTATCATCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGATGGGaa  >  1:238177/1‑150 (MQ=255)
                                                                                                                                           ccgcTGAACCGCCTAACCCGCTGGGCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGaa  <  1:729477/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
GGCCTGCGTGAGCTGAATATCTGCACCGGCTGTGGTCCGGGAGCGATGGAAGCGCCGATGAAAGGTGCTGCGGTCGGACACGCGCAGCAGCGTTACAAAGACAGTCGTTTTATTGGTATGACAGAGCCGTCGATTATCGCCGCTGAACCGCCTAACCCGCTGGTCAACGAATTGATCATCATGCCAGATATCGAAAAACGTCTGGAAGCGTTTGTCCGTATCGCTCACGGTATCATTATCTTCCCTGGCGGTGTGGGTACGGCAGAAGAGTTGCTCTATTTGCTGGGAA  >  NC_000913/2926830‑2927118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑