Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,146,762 G→A 5.3% intergenic (‑25/+94) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,146,7620GA 5.3% 357.8 / 5.7 113intergenic (‑25/+94)yghW/yghXDUF2623 family protein/pseudogene, dienlactone hydrolase family
Reads supporting (aligned to +/- strand):  ref base G (50/57);  new base A (3/3);  total (53/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

Only 100 of 116 total aligned reads displayed.

GGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGG  >  NC_000913/3146614‑3146911
                                                                                                                                                    |                                                                                                                                                     
cgtgtGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTCTCGGCATGCGTTGCTTTTAATCCCGCCATTAACCCATTACCAAAATGCTTATACATCGATCACCTCATTCGCTATAAATTga                                                                                                                                                      >  2:885810/2‑149 (MQ=255)
 gtgtGAGTAGCCTAATACAAAACCGCGTTTATAATCGGAACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTc                                                                                                                                                     <  1:804032/150‑1 (MQ=255)
     gAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTAATCGTTCACCTCATTCGCTATATATTGTCatat                                                                                                                                                 >  2:695250/1‑150 (MQ=255)
      aGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCatata                                                                                                                                                >  2:1051897/1‑150 (MQ=255)
         aGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAg                                                                                                                                             <  2:435872/150‑1 (MQ=255)
         aGCCTAATACAAAACCGCGTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGc                                                                                                                                            <  2:1182311/150‑1 (MQ=255)
          gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                                            <  1:1216226/150‑1 (MQ=255)
           ccTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCGTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                                                                                                                                           <  1:1068659/150‑1 (MQ=255)
            cTAAAACAAAACCGCGTTTATAATCGGCACAGAATTATGACACATTAACCGCACAGTCGGCATGCGATGCTTTTAATCCCGCCATTAAGCCATGACCATAATGGATTTACAACGATCACCTCATTCGCTAAATATTGTCATATATAGCGa                                                                                                                                          >  1:179205/1‑150 (MQ=255)
             tAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaa                                                                                                                                         <  1:275984/150‑2 (MQ=255)
                tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTATTCCCGGCATTAAGCCGTTTGCAAAATGGATATTCTACGTTCACCTCATTCGCTATATATTGACATATATTGCGATAtt                                                                                                                                      >  2:216580/1‑150 (MQ=255)
                 aCAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTTATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatgag                                                                                                                                     <  1:605049/150‑6 (MQ=255)
                    aaaGCGCTTTTATAATCGTCTCAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGGCATTAAGCCTTTACCAAACTGGTTTTTCATCGTTGACCTCATTCGCTAAATATTGTCATATATAGCGATATTTTAg                                                                                                                                  <  1:1318736/150‑1 (MQ=255)
                    aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCAGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAg                                                                                                                                  <  2:1120781/150‑1 (MQ=255)
                     aaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATTTTGTCATATATAGCGATATTTTAgg                                                                                                                                 >  2:1034300/1‑150 (MQ=255)
                      aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                <  2:1060241/150‑1 (MQ=255)
                      aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                <  2:983535/150‑1 (MQ=255)
                          cgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAg                                                                                                                            <  1:427919/150‑1 (MQ=255)
                          cgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAg                                                                                                                            <  1:1228468/150‑1 (MQ=255)
                          cgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAg                                                                                                                            >  2:284162/1‑150 (MQ=255)
                           gTTTATAATCGGCACAGAATTTTGTCACATTAAGCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGa                                                                                                                           <  1:1101442/150‑1 (MQ=255)
                           gTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGa                                                                                                                           <  1:669632/150‑1 (MQ=255)
                                taATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATAGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGAc                                                                                                                      <  1:1124687/150‑1 (MQ=255)
                                 aaTCGGCACAGAATTTTGTAACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACt                                                                                                                     <  1:173227/150‑1 (MQ=255)
                                   tCGGCACAGAATTTTGTCACAATAACCGCACTGTCGGGATGCGTGGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACttt                                                                                                                   <  1:275244/150‑1 (MQ=255)
                                    cGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACtttt                                                                                                                  >  1:833039/1‑150 (MQ=255)
                                     ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                 >  1:355774/1‑150 (MQ=255)
                                     ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                 <  1:734927/150‑1 (MQ=255)
                                     ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                 >  2:360845/1‑150 (MQ=255)
                                      gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgc                                                                                                                <  1:594146/150‑1 (MQ=255)
                                      gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGGTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgc                                                                                                                >  2:903624/1‑150 (MQ=255)
                                       cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCCTCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcg                                                                                                               <  2:703061/150‑1 (MQ=255)
                                        acaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcgc                                                                                                              <  1:344389/150‑1 (MQ=255)
                                             aTTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCt                                                                                                         <  2:355774/150‑1 (MQ=255)
                                              ttttGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTa                                                                                                        >  1:628018/1‑150 (MQ=255)
                                                  gTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAtttt                                                                                                    >  1:972551/1‑150 (MQ=255)
                                                    cacaTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGTTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAtttttt                                                                                                  >  2:1048958/1‑150 (MQ=255)
                                                      caTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                >  2:1256430/1‑150 (MQ=255)
                                                      caTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                >  2:581246/1‑150 (MQ=255)
                                                          aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATTTAGCGATATTTTAGGCAAAGAACGACGTTTGCGCACGCTATTTTTACATGCt                                                                                            >  1:242447/1‑150 (MQ=255)
                                                          aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                            >  1:545012/1‑150 (MQ=255)
                                                          aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                            >  1:1157405/1‑150 (MQ=255)
                                                          aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                            <  1:288486/150‑1 (MQ=255)
                                                            ccGCACTGTCGGCATGCGTTGCTTTTAAACCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCTATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTATCATGCTTg                                                                                          >  2:841787/1‑150 (MQ=255)
                                                             cGCACTGTCGTCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGt                                                                                         <  1:1015449/150‑1 (MQ=255)
                                                             cGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGt                                                                                         <  1:832904/150‑1 (MQ=255)
                                                                   gTCGGCATGCGTTGCTTTTAATCCCGCCATTTAGCCTTTACCAAAATGGTTATTCATAGTTCTCGTCATTCGCTTTATATTGTCATTTATAGCGATATTTTAGGCAAAGAACGACTTTTGCGGACGCTATTTTTTCATGCTTGTTTAAgt                                                                                   <  1:430127/150‑2 (MQ=255)
                                                                    tCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGAc                                                                                  <  2:131515/150‑1 (MQ=255)
                                                                       gCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTa                                                                               >  2:658109/1‑150 (MQ=255)
                                                                       gCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTa                                                                               >  1:1020710/1‑150 (MQ=255)
                                                                          tGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATc                                                                            >  2:718026/1‑150 (MQ=255)
                                                                           gCGTTGCTTTTAATCCCGCCATTTAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTTTTTATTGTCATATATAGCGATATTTTAGGCAAAGACCGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATcg                                                                           <  2:622591/150‑2 (MQ=255)
                                                                               tGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTa                                                                       >  2:405681/1‑150 (MQ=255)
                                                                                gCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaa                                                                      <  1:297809/150‑1 (MQ=255)
                                                                                 cTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTAACCTCATTCGCTATATATTGTCATATAAAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACTCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaaa                                                                     >  1:663931/1‑149 (MQ=255)
                                                                                  ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCTCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTAATGCTTGTTTAAGACGTAATCCCGTAaga                                                                    >  1:938917/1‑150 (MQ=255)
                                                                                      aaTCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATATTAGGCAAAGAACCACTTTTGCGCACGCTATTTATACATGCTTGTTTAAGAAGTAATCCAGTAAGAGATa                                                                >  2:1341539/1‑150 (MQ=255)
                                                                                        tCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATaaa                                                              >  1:458167/1‑150 (MQ=255)
                                                                                         cccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCTTAAGAGATAAAc                                                             >  1:895780/1‑150 (MQ=255)
                                                                                         cccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATaacc                                                             >  1:998322/1‑148 (MQ=255)
                                                                                          ccGCCATTTAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGTGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAcc                                                            <  2:728230/150‑1 (MQ=255)
                                                                                          ccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAca                                                            >  1:1327094/1‑149 (MQ=255)
                                                                                              cATTAAGCCTTTACCATAATGGTTATTCATCGTTCACCTCATTCTCTATTTATTGTCATATATAGCGTTATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATc                                                        <  2:1316889/150‑1 (MQ=255)
                                                                                               aTTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCa                                                       >  1:944608/1‑150 (MQ=255)
                                                                                               aTTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCa                                                       <  1:1175411/150‑1 (MQ=255)
                                                                                                   aGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGgag                                                   <  2:1284806/150‑1 (MQ=255)
                                                                                                       tttACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGat                                               >  2:576979/1‑150 (MQ=255)
                                                                                                         tACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGatat                                             >  1:279526/1‑150 (MQ=255)
                                                                                                         tACCAAAATGGTTATTCATCGTTCACATCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGatat                                             >  1:428699/1‑150 (MQ=255)
                                                                                                           ccAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGGTTTTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATAttt                                           >  2:479468/1‑150 (MQ=255)
                                                                                                              aaaTGCTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATc                                        <  2:188178/150‑1 (MQ=255)
                                                                                                               aaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCg                                       <  2:1032534/150‑1 (MQ=255)
                                                                                                               aaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCg                                       <  1:364084/150‑1 (MQ=255)
                                                                                                               aaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATAAACCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCg                                       <  2:1266368/150‑1 (MQ=255)
                                                                                                                     tATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCAt                                 >  2:796996/1‑150 (MQ=255)
                                                                                                                        tCATCGTTCACATCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGCACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTATGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTc                              <  2:1129713/150‑1 (MQ=255)
                                                                                                                         cATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                             >  1:901183/1‑150 (MQ=255)
                                                                                                                          aTCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAg                            <  2:1160314/150‑1 (MQ=255)
                                                                                                                          aTCGTTCAACTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAg                            <  1:1151395/150‑1 (MQ=255)
                                                                                                                            cGTTCACCTCTTTAGCTATATATTGTCATATATAGAGATATTTTAGGCAAAGAACGACTTTTGCGCACTCTATTTTTTCATTCTTGTTTAAGAAGTAATCCCTTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtg                          <  1:841787/150‑1 (MQ=255)
                                                                                                                            cGTTCACCTCATTCGCTATATAATGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGAAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtg                          <  1:657944/150‑1 (MQ=255)
                                                                                                                             gTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATGCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                         >  1:1077455/1‑150 (MQ=255)
                                                                                                                             gTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                         <  2:833039/150‑1 (MQ=255)
                                                                                                                             gTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                         >  2:618631/1‑150 (MQ=255)
                                                                                                                                cACCTTATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCt                      <  2:646171/150‑1 (MQ=255)
                                                                                                                                 aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCtt                     <  2:1121939/150‑1 (MQ=255)
                                                                                                                                 aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCATGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACAATTTCAGTGTCCtt                     >  1:106595/1‑150 (MQ=255)
                                                                                                                                  ccTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                    >  1:711612/1‑150 (MQ=255)
                                                                                                                                  ccTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                    >  2:477093/1‑150 (MQ=255)
                                                                                                                                   cTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTg                   >  2:1012261/1‑150 (MQ=255)
                                                                                                                                     cATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGcc                 <  1:563072/150‑1 (MQ=255)
                                                                                                                                      aTTCGCTATATATTGTCATATATAGCGATATTTTAGTCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATTTTTATCGAACCATTTCAGTGTCGTTTGCCa                <  2:979728/150‑1 (MQ=255)
                                                                                                                                      aTTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCa                <  2:1227494/150‑1 (MQ=255)
                                                                                                                                        tCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAgg              <  1:392031/150‑1 (MQ=255)
                                                                                                                                          gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCa            >  1:1013589/1‑150 (MQ=255)
                                                                                                                                          gCTATATATGACAATATATAGAGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGGTTGTTTAAGACGTAATACCGTAAGAGAAAAACCTATAAGGAGTGATATTTATAGAACCATTTCAGTGTCCTTTGCCAGGCa            >  2:790909/1‑150 (MQ=255)
                                                                                                                                           cTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTATGCGAACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCaa           >  1:185782/1‑150 (MQ=255)
                                                                                                                                                 atTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTAAAGACGTAATCACGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGGCAGGCAAGATcgg     >  1:524187/1‑150 (MQ=255)
                                                                                                                                                    gTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAACCCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  >  2:393299/1‑150 (MQ=255)
                                                                                                                                                    gTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg  <  1:707656/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGG  >  NC_000913/3146614‑3146911

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: