Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 156,519 G→T 5.2% P122Q (CCA→CAA)  yadN ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913156,5190GT 5.2% 341.3 / 2.4 123P122Q (CCA→CAA) yadNputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (52/62);  new base T (5/3);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

GAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAAC  >  NC_000913/156373‑156667
                                                                                                                                                  |                                                                                                                                                    
gAACGTAAGACGCTTTAAAATCATAACCAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgt                                                                                                                                                   <  2:900589/150‑1 (MQ=255)
gAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgt                                                                                                                                                   <  2:1023852/150‑1 (MQ=255)
  aCGTAATACGCTTTAATATCATAAACAGCAGATTTTGTCGTTGCATGAAGGGCTTTAGTATACACATCTCCAGGGTTGTTAATTTTAACCTGTTTGATATGGGTACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgttg                                                                                                                                                 <  2:934540/150‑1 (MQ=255)
   cGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGGTTTAGTATAAACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCAACAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgttgt                                                                                                                                                >  1:1352466/1‑150 (MQ=255)
    gTAAGACGCTTTAAAATCATAAACAGCATATTTAGTCGTTGCATCCAGGGCTTTAGTATACACAGCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgttgtt                                                                                                                                               <  2:657144/150‑1 (MQ=255)
    gTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGGTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGgttgtt                                                                                                                                               <  1:82891/150‑1 (MQ=255)
       aGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACAAATCGCCAGGGTTGGTAATTTGAACCTGTTTGATAGTGGAACCATCAAGATTGTTTAGAGCGATATTAAAGCCGTCAGATGGGTTGTTAAt                                                                                                                                            >  1:84130/1‑150 (MQ=255)
        taCGCTTTATAATCATAAACAGCAGATTTTGTCGTTGCATGCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGATCCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATa                                                                                                                                           <  1:373513/149‑1 (MQ=255)
        gACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATa                                                                                                                                           <  1:1043510/150‑1 (MQ=255)
         aCGCTTTAAAATCATAAACAGCAGATTTTGTCGTTTCATCCAGTGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTTGATCCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAg                                                                                                                                          <  1:340368/150‑1 (MQ=255)
         aCGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAg                                                                                                                                          <  1:1045267/150‑1 (MQ=255)
           gCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGAc                                                                                                                                        <  1:715626/150‑1 (MQ=255)
            cTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCTATATTAACGCCGTCAGATGGGTTGTTAATAGACa                                                                                                                                       >  1:985582/1‑150 (MQ=255)
             tttAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCTTCAGATGGGTTGTTAATAGACAt                                                                                                                                      >  1:568362/1‑150 (MQ=255)
                aaaaTCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTc                                                                                                                                   >  2:394500/1‑150 (MQ=255)
                aaaaTCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAAAAGACATGTc                                                                                                                                   >  2:33229/1‑150 (MQ=255)
                 aaaTCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCg                                                                                                                                  <  1:1233434/150‑1 (MQ=255)
                 aaaTCATAAACAGCAGATTTTGTCGTAGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATATGAACATGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGATGTTAATATAAATGTCg                                                                                                                                  >  2:417153/1‑150 (MQ=255)
                   aTCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGCCATGTCgtt                                                                                                                                >  2:220490/1‑150 (MQ=255)
                   aTCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCgtt                                                                                                                                >  1:329477/1‑150 (MQ=255)
                       tAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTc                                                                                                                            >  1:983719/1‑150 (MQ=255)
                           cagcagATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAg                                                                                                                        <  2:157826/150‑1 (MQ=255)
                           cagcagATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAg                                                                                                                        <  1:1111308/150‑1 (MQ=255)
                                gATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCt                                                                                                                   >  2:42309/1‑150 (MQ=255)
                                 aTTTTGTCGTTGCATCTAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGATCTATATTAACGCCGTCAAATTATATCTTAATAGAAATGTCTTTGTTCAAAGTCCAtt                                                                                                                  >  2:1197580/1‑150 (MQ=255)
                                 aTTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCtt                                                                                                                  <  2:954169/150‑1 (MQ=255)
                                   tttGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTg                                                                                                                <  1:338103/150‑1 (MQ=255)
                                   tttGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTg                                                                                                                <  2:974881/150‑1 (MQ=255)
                                     tGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCt                                                                                                              >  1:588114/1‑150 (MQ=255)
                                     tGTCGTTGCATCCAGGGCTTTAGTATACACATCGCAAGTGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCt                                                                                                              <  1:238384/150‑1 (MQ=255)
                                     tGTCGTTGCATCCAGGGATTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGTTAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCt                                                                                                              <  2:321140/150‑1 (MQ=255)
                                      gTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTg                                                                                                             <  1:1104538/150‑1 (MQ=255)
                                         gTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTa                                                                                                          <  1:658145/150‑1 (MQ=255)
                                         gTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGAGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTa                                                                                                          >  1:671076/1‑150 (MQ=255)
                                            gCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCa                                                                                                       <  1:280148/150‑1 (MQ=255)
                                            gCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCa                                                                                                       >  2:272099/1‑150 (MQ=255)
                                               tCCAGGTCTTTAGTATAAACATAGCAATGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATAAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAg                                                                                                    <  2:660236/150‑1 (MQ=255)
                                               tCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGCACCTGTTTGATAGTGGAACCCTCAATATTGTGTAGAGCGATATTAACGCCGTCAGATTGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAg                                                                                                    <  2:660235/150‑1 (MQ=255)
                                                ccAGTGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTTTGTACAGCGATATTAACGCCGTCAGATGGGTTTTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGa                                                                                                   <  1:333505/150‑1 (MQ=255)
                                                 cAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGCCATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGaa                                                                                                  >  1:340233/1‑150 (MQ=255)
                                                    ggCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAAc                                                                                               <  2:1267048/150‑1 (MQ=255)
                                                      cTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTTTGTAGAGCGATATTAACGCAGTCATATGGGTAGTTAATAGACATATCGTAGTTCAAAGTACCTTTGCTGTTACCAAAGAAAACAg                                                                                             >  1:208276/1‑150 (MQ=255)
                                                       tttAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTAGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGa                                                                                            <  1:834995/150‑1 (MQ=255)
                                                          aGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAAcc                                                                                         <  2:326553/150‑1 (MQ=255)
                                                               acacATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGGGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAgg                                                                                    <  2:210736/150‑1 (MQ=255)
                                                                cacaTCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGATCGATATTAACGCCGTCAAATGTGTTTTAAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGt                                                                                   >  2:156692/1‑150 (MQ=255)
                                                                cacaTCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGt                                                                                   <  2:694277/150‑1 (MQ=255)
                                                                cacaTCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGAGTAGATCTCTATTAACGCCGTAACATAAATTAAAAATAGACATGTCGTTGTTCAAATTCCCTTTGCTGTTACCAAAGAAAACAGCACCGAAggg                                                                                   >  2:1168737/1‑149 (MQ=255)
                                                                      gCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCAtttt                                                                             >  2:1108767/1‑150 (MQ=255)
                                                                         aGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGTTTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCATAGAAAACAGAACCGAAGGTCATTTTAGc                                                                          >  2:128963/1‑150 (MQ=255)
                                                                         aGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGc                                                                          >  2:579802/1‑150 (MQ=255)
                                                                          gggTTGTTAATTTGAACCTGTTTGATAGTGGAACGATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGTGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGcc                                                                         <  1:205930/150‑1 (MQ=255)
                                                                          gggTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTTGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCATAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGcc                                                                         >  1:1005767/1‑150 (MQ=255)
                                                                             gtgttAATTTGAACCTGGTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTg                                                                      <  1:906081/149‑1 (MQ=255)
                                                                               gttAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCt                                                                    >  2:853868/1‑150 (MQ=255)
                                                                                ttAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGATCTATATTTACGCCGTATCATTAAATAAAAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAAACGAAGGTCATTTTAGCGGTGcga                                                                   >  2:137605/1‑148 (MQ=255)
                                                                                 tAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAc                                                                  >  1:464533/1‑150 (MQ=255)
                                                                                  aaTTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGATCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTAcc                                                                 <  1:1237857/150‑1 (MQ=255)
                                                                                      tGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGATCTATATTAACGCCGTAACATTAAAAAAAAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGa                                                             >  2:615430/1‑150 (MQ=255)
                                                                                       gAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCTATATTAACGCCGTCAGATTGTTAGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGAt                                                            >  2:204663/1‑150 (MQ=255)
                                                                                       gAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGAt                                                            >  1:719369/1‑150 (MQ=255)
                                                                                         aCCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCTATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTAAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTc                                                          >  1:812059/1‑150 (MQ=255)
                                                                                          ccTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCg                                                         <  2:983719/150‑1 (MQ=255)
                                                                                          ccTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCg                                                         >  1:1266918/1‑150 (MQ=255)
                                                                                          ccTGTTTGATAGTGGAACCATCAATAATGTGTAGAGCGATATTACCGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCg                                                         <  2:1288222/150‑1 (MQ=255)
                                                                                           cTGTTTGATAGTGGAACCATCAATATTGTGTAGATCTCTATTAACGCCGTCACATTAAAAAAAAAAAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCgg                                                        >  2:796817/1‑150 (MQ=255)
                                                                                            tGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGa                                                       >  2:367345/1‑150 (MQ=255)
                                                                                             tttttATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAt                                                      <  2:551866/149‑1 (MQ=255)
                                                                                               ttGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAttt                                                    <  1:487207/150‑1 (MQ=255)
                                                                                                tGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttg                                                   <  1:170589/150‑1 (MQ=255)
                                                                                                   tAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctt                                                <  2:1303596/150‑1 (MQ=255)
                                                                                                   tAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctt                                                >  2:717222/1‑150 (MQ=255)
                                                                                                      tGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGAtttgctttgc                                             <  1:32384/150‑1 (MQ=255)
                                                                                                       ggAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATttgctttgct                                            >  1:615064/1‑150 (MQ=255)
                                                                                                        gAACCATCTATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTGGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTtgctg                                           >  1:678710/1‑150 (MQ=255)
                                                                                                        gAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTtgctg                                           >  2:595271/1‑150 (MQ=255)
                                                                                                           ccATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACCGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAg                                        <  2:135691/150‑1 (MQ=255)
                                                                                                           ccATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAg                                        <  1:128701/150‑1 (MQ=255)
                                                                                                            cATCAATATTGTGTAGAGCGATATTAACGCCGTCGGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGt                                       >  1:341001/1‑150 (MQ=255)
                                                                                                                aaTATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc                                   >  1:1030636/1‑150 (MQ=255)
                                                                                                                aaTATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAAc                                   >  2:1243222/1‑150 (MQ=255)
                                                                                                                 atatTGTGTAGATCGATATTAACTGCGTCAGATGGGTTTTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACg                                  <  1:33229/150‑1 (MQ=255)
                                                                                                                 atatTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACg                                  <  1:460008/150‑1 (MQ=255)
                                                                                                                   atTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGt                                >  1:103464/1‑150 (MQ=255)
                                                                                                                   atTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGt                                >  1:583288/1‑150 (MQ=255)
                                                                                                                       tgtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATg                            <  2:588114/150‑1 (MQ=255)
                                                                                                                        gtAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGGGATGc                           <  1:443935/150‑1 (MQ=255)
                                                                                                                          agagCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTg                         >  2:778447/1‑150 (MQ=255)
                                                                                                                          agagCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTg                         >  2:1097483/1‑150 (MQ=255)
                                                                                                                          agagCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTCCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTg                         >  2:227815/1‑150 (MQ=255)
                                                                                                                               tatatTAACGCCGTCAGATGGGTTGTTAATAGTCATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTTCCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAgg                    <  2:724306/149‑1 (MQ=255)
                                                                                                                               gATATTAACGCCGTCAGATGGGTTGATAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAgg                    >  1:899205/1‑150 (MQ=255)
                                                                                                                                atatTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACAAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGt                   >  1:1245911/1‑150 (MQ=255)
                                                                                                                                  atTATCCCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTACCTTTGCTGTTACCAAAGAAATCAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGttt                 <  2:888173/150‑1 (MQ=255)
                                                                                                                                    tAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttag               <  1:1108767/150‑1 (MQ=255)
                                                                                                                                    tAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGGCATGTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttag               <  2:899782/150‑1 (MQ=255)
                                                                                                                                    aaaCGCCGTCAGATTGGGTGTTAATAGCCATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTTGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttag               <  2:465933/149‑1 (MQ=255)
                                                                                                                                          cGTAAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACAGAAGGTCATTTTAGCCGTGCTACCTGGATTCGTATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGtttagctttag         <  1:265467/150‑1 (MQ=255)
                                                                                                                                            tCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAgcg       <  1:224022/150‑1 (MQ=255)
                                                                                                                                                 tGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAAc  <  1:1107853/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAACGTAAGACGCTTTAAAATCATAAACAGCAGATTTTGTCGTTGCATCCAGGGCTTTAGTATACACATCGCCAGGGTTGTTAATTTGAACCTGTTTGATAGTGGAACCATCAATATTGTGTAGAGCGATATTAACGCCGTCAGATGGGTTGTTAATAGACATGTCGTTGTTCAAAGTCCCTTTGCTGTTACCAAAGAAAACAGAACCGAAGGTCATTTTAGCCGTGCTACCTGGATTCGGATTTGCTTTGCTGCAGTCAACGGTGATGCTGAAAGGTTTAGCTTTAGCGCCAAC  >  NC_000913/156373‑156667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: