Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,146,763 T→C 5.3% intergenic (‑26/+93) yghW ← / ← yghX DUF2623 family protein/pseudogene, dienlactone hydrolase family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,146,7630TC 5.3% 345.9 / 7.5 115intergenic (‑26/+93)yghW/yghXDUF2623 family protein/pseudogene, dienlactone hydrolase family
Reads supporting (aligned to +/- strand):  ref base T (50/58);  new base C (3/3);  total (54/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

Only 100 of 116 total aligned reads displayed.

GTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGC  >  NC_000913/3146615‑3146912
                                                                                                                                                    |                                                                                                                                                     
gtgtGAGTAGCCTAATACAAAACCGCGTTTATAATCGGAACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTc                                                                                                                                                      <  1:804032/150‑1 (MQ=255)
    gAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTAATCGTTCACCTCATTCGCTATATATTGTCatat                                                                                                                                                  >  2:695250/1‑150 (MQ=255)
     aGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCatata                                                                                                                                                 >  2:1051897/1‑150 (MQ=255)
        aGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAg                                                                                                                                              <  2:435872/150‑1 (MQ=255)
        aGCCTAATACAAAACCGCGTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGc                                                                                                                                             <  2:1182311/150‑1 (MQ=255)
         gCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGc                                                                                                                                             <  1:1216226/150‑1 (MQ=255)
          ccTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCGTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCg                                                                                                                                            <  1:1068659/150‑1 (MQ=255)
           cTAAAACAAAACCGCGTTTATAATCGGCACAGAATTATGACACATTAACCGCACAGTCGGCATGCGATGCTTTTAATCCCGCCATTAAGCCATGACCATAATGGATTTACAACGATCACCTCATTCGCTAAATATTGTCATATATAGCGa                                                                                                                                           >  1:179205/1‑150 (MQ=255)
            tAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaa                                                                                                                                          <  1:275984/150‑2 (MQ=255)
               tACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTATTCCCGGCATTAAGCCGTTTGCAAAATGGATATTCTACGTTCACCTCATTCGCTATATATTGACATATATTGCGATAtt                                                                                                                                       >  2:216580/1‑150 (MQ=255)
                aCAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTTATCGTTCACCTCATTCGCTATATATGACAATATATAGCGaatgag                                                                                                                                      <  1:605049/150‑6 (MQ=255)
                   aaaGCGCTTTTATAATCGTCTCAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGGCATTAAGCCTTTACCAAACTGGTTTTTCATCGTTGACCTCATTCGCTAAATATTGTCATATATAGCGATATTTTAg                                                                                                                                   <  1:1318736/150‑1 (MQ=255)
                   aaaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCAGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAg                                                                                                                                   <  2:1120781/150‑1 (MQ=255)
                    aaCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATTTTGTCATATATAGCGATATTTTAgg                                                                                                                                  >  2:1034300/1‑150 (MQ=255)
                     aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                 <  2:983535/150‑1 (MQ=255)
                     aCCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGc                                                                                                                                 <  2:1060241/150‑1 (MQ=255)
                         cgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAg                                                                                                                             <  1:1228468/150‑1 (MQ=255)
                         cgTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAg                                                                                                                             <  1:427919/150‑1 (MQ=255)
                          gTTTATAATCGGCACAGAATTTTGTCACATTAAGCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGa                                                                                                                            <  1:1101442/150‑1 (MQ=255)
                          gTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGa                                                                                                                            <  1:669632/150‑1 (MQ=255)
                               taATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATAGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGAc                                                                                                                       <  1:1124687/150‑1 (MQ=255)
                                aaTCGGCACAGAATTTTGTAACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACt                                                                                                                      <  1:173227/150‑1 (MQ=255)
                                  tCGGCACAGAATTTTGTCACAATAACCGCACTGTCGGGATGCGTGGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACttt                                                                                                                    <  1:275244/150‑1 (MQ=255)
                                   cGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACtttt                                                                                                                   >  1:833039/1‑150 (MQ=255)
                                    ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                  >  2:360845/1‑150 (MQ=255)
                                    ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                  <  1:734927/150‑1 (MQ=255)
                                    ggCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTg                                                                                                                  >  1:355774/1‑150 (MQ=255)
                                     gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgc                                                                                                                 <  1:594146/150‑1 (MQ=255)
                                     gCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGGTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgc                                                                                                                 >  2:903624/1‑150 (MQ=255)
                                      cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCCTCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcg                                                                                                                <  2:703061/150‑1 (MQ=255)
                                      cacaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcg                                                                                                                <  2:662092/150‑1 (MQ=255)
                                       acaGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTgcgc                                                                                                               <  1:344389/150‑1 (MQ=255)
                                            aTTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCt                                                                                                          <  2:355774/150‑1 (MQ=255)
                                             ttttGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTa                                                                                                         >  1:628018/1‑150 (MQ=255)
                                                 gTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAtttt                                                                                                     >  1:972551/1‑150 (MQ=255)
                                                   cacaTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGTTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTAtttttt                                                                                                   >  2:1048958/1‑150 (MQ=255)
                                                     caTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                 >  2:1256430/1‑150 (MQ=255)
                                                     caTTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCa                                                                                                 >  2:581246/1‑150 (MQ=255)
                                                         aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATTTAGCGATATTTTAGGCAAAGAACGACGTTTGCGCACGCTATTTTTACATGCt                                                                                             >  1:242447/1‑150 (MQ=255)
                                                         aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                             >  1:1157405/1‑150 (MQ=255)
                                                         aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                             >  1:545012/1‑150 (MQ=255)
                                                         aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                             >  2:1309369/1‑150 (MQ=255)
                                                         aaCCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCt                                                                                             <  1:288486/150‑1 (MQ=255)
                                                           ccGCACTGTCGGCATGCGTTGCTTTTAAACCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCTATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTATCATGCTTg                                                                                           >  2:841787/1‑150 (MQ=255)
                                                            cGCACTGTCGTCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGt                                                                                          <  1:1015449/150‑1 (MQ=255)
                                                            cGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGt                                                                                          <  1:832904/150‑1 (MQ=255)
                                                                  gTCGGCATGCGTTGCTTTTAATCCCGCCATTTAGCCTTTACCAAAATGGTTATTCATAGTTCTCGTCATTCGCTTTATATTGTCATTTATAGCGATATTTTAGGCAAAGAACGACTTTTGCGGACGCTATTTTTTCATGCTTGTTTAAgt                                                                                    <  1:430127/150‑2 (MQ=255)
                                                                   tCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGAc                                                                                   <  2:131515/150‑1 (MQ=255)
                                                                      gCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTa                                                                                >  1:1020710/1‑150 (MQ=255)
                                                                         tGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATc                                                                             >  2:718026/1‑150 (MQ=255)
                                                                          gCGTTGCTTTTAATCCCGCCATTTAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTTTTTATTGTCATATATAGCGATATTTTAGGCAAAGACCGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATcg                                                                            <  2:622591/150‑2 (MQ=255)
                                                                             ttGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGt                                                                         <  2:336964/150‑1 (MQ=255)
                                                                              tGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTa                                                                        >  2:405681/1‑150 (MQ=255)
                                                                               gCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaa                                                                       <  1:297809/150‑1 (MQ=255)
                                                                                cTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTAACCTCATTCGCTATATATTGTCATATAAAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACTCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTaaa                                                                      >  1:663931/1‑149 (MQ=255)
                                                                                 ttttAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCTCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTAATGCTTGTTTAAGACGTAATCCCGTAaga                                                                     >  1:938917/1‑150 (MQ=255)
                                                                                     aaTCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATATTAGGCAAAGAACCACTTTTGCGCACGCTATTTATACATGCTTGTTTAAGAAGTAATCCAGTAAGAGATa                                                                 >  2:1341539/1‑150 (MQ=255)
                                                                                       tCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATaaa                                                               >  1:458167/1‑150 (MQ=255)
                                                                                        cccGCCATTAAGCCTTTACCAAGATGGTTATTCATAGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAc                                                              >  2:563072/1‑150 (MQ=255)
                                                                                        cccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCTTAAGAGATAAAc                                                              >  1:895780/1‑150 (MQ=255)
                                                                                        cccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATaacc                                                              >  1:998322/1‑148 (MQ=255)
                                                                                         ccGCCATTTAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGTGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAcc                                                             <  2:728230/150‑1 (MQ=255)
                                                                                         ccGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAAca                                                             >  1:1327094/1‑149 (MQ=255)
                                                                                             cATTAAGCCTTTACCATAATGGTTATTCATCGTTCACCTCATTCTCTATTTATTGTCATATATAGCGTTATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATc                                                         <  2:1316889/150‑1 (MQ=255)
                                                                                              aTTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCa                                                        >  1:944608/1‑150 (MQ=255)
                                                                                              aTTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCa                                                        <  1:1175411/150‑1 (MQ=255)
                                                                                                      tttACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGat                                                >  2:576979/1‑150 (MQ=255)
                                                                                                        tACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGatat                                              >  1:279526/1‑150 (MQ=255)
                                                                                                        tACCAAAATGGTTATTCATCGTTCACATCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGatat                                              >  1:428699/1‑150 (MQ=255)
                                                                                                             aaaTGCTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATc                                         <  2:188178/150‑1 (MQ=255)
                                                                                                              aaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCg                                        <  2:1032534/150‑1 (MQ=255)
                                                                                                              aaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCg                                        <  1:364084/150‑1 (MQ=255)
                                                                                                              aaTGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATAAACCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCg                                        <  2:1266368/150‑1 (MQ=255)
                                                                                                                        cATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCa                              >  1:901183/1‑150 (MQ=255)
                                                                                                                         aTCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAg                             <  2:1160314/150‑1 (MQ=255)
                                                                                                                         aTCGTTCAACTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAg                             <  1:1151395/150‑1 (MQ=255)
                                                                                                                           cGTTCACCTCTTTAGCTATATATTGTCATATATAGAGATATTTTAGGCAAAGAACGACTTTTGCGCACTCTATTTTTTCATTCTTGTTTAAGAAGTAATCCCTTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtg                           <  1:841787/150‑1 (MQ=255)
                                                                                                                           cGTTCACCTCATTCGCTATATAATGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGAAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtg                           <  1:657944/150‑1 (MQ=255)
                                                                                                                            gTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATGCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                          >  1:1077455/1‑150 (MQ=255)
                                                                                                                            gTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                          <  2:833039/150‑1 (MQ=255)
                                                                                                                            gTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAgtgt                          >  2:618631/1‑150 (MQ=255)
                                                                                                                               cACCTTATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCt                       <  2:646171/150‑1 (MQ=255)
                                                                                                                                aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCtt                      <  2:1121939/150‑1 (MQ=255)
                                                                                                                                aCCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCATGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACAATTTCAGTGTCCtt                      >  1:106595/1‑150 (MQ=255)
                                                                                                                                 ccTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                     >  1:711612/1‑150 (MQ=255)
                                                                                                                                 ccTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCttt                     >  2:477093/1‑150 (MQ=255)
                                                                                                                                  cTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTg                    >  2:1012261/1‑150 (MQ=255)
                                                                                                                                    cATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGcc                  <  1:563072/150‑1 (MQ=255)
                                                                                                                                     aTTCGCTATATATTGTCATATATAGCGATATTTTAGTCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATTTTTATCGAACCATTTCAGTGTCGTTTGCCa                 <  2:979728/150‑1 (MQ=255)
                                                                                                                                     aTTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCa                 <  2:1227494/150‑1 (MQ=255)
                                                                                                                                       tCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAgg               <  1:392031/150‑1 (MQ=255)
                                                                                                                                       tCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAgg               <  2:1158773/150‑1 (MQ=255)
                                                                                                                                        cGCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACTACTATTGCGCAAGCTATTTTTTCATGCTTTTTTAAGACGTAATCCCGTAAGATATAAACCTATCAGTAGAGATATTTATCGAACAATTTCAGTCTCCTTAGCCAGGc              >  2:816378/1‑150 (MQ=255)
                                                                                                                                         gCTATATATGACAATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCa             >  1:1013589/1‑150 (MQ=255)
                                                                                                                                         gCTATATATGACAATATATAGAGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGGTTGTTTAAGACGTAATACCGTAAGAGAAAAACCTATAAGGAGTGATATTTATAGAACCATTTCAGTGTCCTTTGCCAGGCa             >  2:790909/1‑150 (MQ=255)
                                                                                                                                          cTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTATGCGAACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCaa            >  1:185782/1‑150 (MQ=255)
                                                                                                                                                atTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTAAAGACGTAATCACGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGGCAGGCAAGATcgg      >  1:524187/1‑150 (MQ=255)
                                                                                                                                                   gTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAACCCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg   >  2:393299/1‑150 (MQ=255)
                                                                                                                                                   gTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATcggcgg   <  1:707656/150‑1 (MQ=255)
                                                                                                                                                    tCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCggcggc  >  2:1119386/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGTGAGTAGCCTAATACAAAACCGCGTTTATAATCGGCACAGAATTTTGTCACATTAACCGCACTGTCGGCATGCGTTGCTTTTAATCCCGCCATTAAGCCTTTACCAAAATGGTTATTCATCGTTCACCTCATTCGCTATATATTGTCATATATAGCGATATTTTAGGCAAAGAACGACTTTTGCGCACGCTATTTTTTCATGCTTGTTTAAGACGTAATCCCGTAAGAGATAAACCTATCAGGAGAGATATTTATCGAACCATTTCAGTGTCCTTTGCCAGGCAAGATCGGCGGC  >  NC_000913/3146615‑3146912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: