Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 217,037 A→T 5.4% intergenic (‑34/+20) yaeF ← / ← proS putative lipoprotein/prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913217,0370AT 5.4% 319.5 / 4.2 106intergenic (‑34/+20)yaeF/proSputative lipoprotein/prolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (52/47);  new base T (3/3);  total (55/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTG  >  NC_000913/216888‑217186
                                                                                                                                                     |                                                                                                                                                     
gCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCAGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTGTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGaaa                                                                                                                                                       >  2:1171417/1‑150 (MQ=255)
  cTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGTCGGCAGTACGCCTTTGGTTTATGCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAAtt                                                                                                                                                     >  2:636069/1‑150 (MQ=255)
    cGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCa                                                                                                                                                   <  2:707587/150‑1 (MQ=255)
     ggCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAg                                                                                                                                                  <  1:1191510/150‑1 (MQ=255)
      gCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAgg                                                                                                                                                 <  1:700361/150‑1 (MQ=255)
      gCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAgg                                                                                                                                                 <  1:129650/150‑1 (MQ=255)
         tCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGAcc                                                                                                                                              <  1:524145/150‑1 (MQ=255)
            aCTGCTGTGGATGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCtt                                                                                                                                           <  1:562030/150‑1 (MQ=255)
              tgctgTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGCCCtttt                                                                                                                                         <  2:830061/150‑2 (MQ=255)
              tgctgTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTc                                                                                                                                         <  1:642199/150‑1 (MQ=255)
                gtgtgTCTGATGGGGCTGGCTGGCTGATATCAACGGTTCAAGCCGAAAGTAAAAGAAAGCTGGGTTGAACCAGGCGGCAGTACTCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTg                                                                                                                                       <  2:1207796/149‑1 (MQ=255)
                 tgtgGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGTCCTGAATTTCAGGGCCCttttttt                                                                                                                                      <  2:1142184/150‑5 (MQ=255)
                 ggtgGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTgg                                                                                                                                      <  2:1087980/149‑1 (MQ=255)
                  gtgGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGc                                                                                                                                     <  1:712097/150‑1 (MQ=255)
                      cTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCa                                                                                                                                 >  2:1320035/1‑150 (MQ=255)
                            ggTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCttt                                                                                                                           <  2:1193642/150‑1 (MQ=255)
                             gTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTa                                                                                                                          >  1:176336/1‑150 (MQ=255)
                               ctggctggCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAAt                                                                                                                        <  1:1284679/150‑1 (MQ=255)
                                 ggctggctGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCt                                                                                                                      >  2:636860/1‑150 (MQ=255)
                                  gctggctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGGCTTTGGTTTATCAATTTTATACAATCCATGTTAAAAAAGGGCCCTGAATGTCAGGCCCCTTTCTGGCAGCCGCCTTTAAGCTg                                                                                                                     >  2:56164/1‑150 (MQ=255)
                                   ctggctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGt                                                                                                                    >  2:91908/1‑150 (MQ=255)
                                   ctggctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGt                                                                                                                    >  1:274813/1‑150 (MQ=255)
                                   ctggctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGt                                                                                                                    <  2:114206/150‑1 (MQ=255)
                                    tggctgATATCAACGGTAAAAGCCGAAAGTAAAAGAAAGCTTGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGtt                                                                                                                   <  2:236021/150‑1 (MQ=255)
                                     ggctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGttt                                                                                                                  >  2:632773/1‑150 (MQ=255)
                                       ctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCa                                                                                                                >  2:1063154/1‑150 (MQ=255)
                                       ctgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCa                                                                                                                >  1:264347/1‑150 (MQ=255)
                                        tgATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCAc                                                                                                               >  2:757307/1‑150 (MQ=255)
                                         gATATCAACGGTACAAGCCGAAAGTCAAAGAACGCTGGCGAGCAACCGGCGGCAGTACGCCTTTGGGGTATACATTTTATACAAACCACGTAAAAACCGGGCCCTGAACTTCAGGACCCTTTCTGGCATCCGCCTTTAATCTGTTTCAcc                                                                                                              <  1:88301/150‑1 (MQ=255)
                                                aCGGTACAAGCCGAAAGTAATTCAAAGCTGGGTAGATACAGGCGGCAGTACGGCTTTGGATTATCAATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAAATCAGGACCCTTTCTGGCATCAGCCTTAAATCTGTTTCACCAGATAtt                                                                                                       <  2:1176586/150‑1 (MQ=255)
                                                 cGGTACAAGCCGAAAGTAAAAGAAAGCGGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTc                                                                                                      <  1:538373/150‑1 (MQ=255)
                                                  ggTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcg                                                                                                     >  1:321922/1‑150 (MQ=255)
                                                     aCAAGCCGAAAGTAAAAGAAAGATGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcgacg                                                                                                  <  2:62769/150‑1 (MQ=255)
                                                        aGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATg                                                                                               >  2:3171/1‑150 (MQ=255)
                                                        aGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATg                                                                                               <  1:632773/150‑1 (MQ=255)
                                                             aaaGTAAAATCCAGCTGGGGAGTAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCAcc                                                                                          <  2:216856/150‑1 (MQ=255)
                                                             aaaGTAAAAGAAAGCTGTGGAGAAACAGGAGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCAcc                                                                                          <  1:530313/150‑1 (MQ=255)
                                                              aaGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCAACa                                                                                         >  1:634319/1‑150 (MQ=255)
                                                               aGTAAAAGAAAGCTGGGGAGAAACAGGTGGCAGTACGCCTTTGGTTTATCCATGTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCGTTCTGGCATCAGCGTTTAATGTGTTTCACCAGATATTCGACGATGTCACCAg                                                                                        >  2:764967/1‑150 (MQ=255)
                                                                gTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGt                                                                                       >  2:289540/1‑150 (MQ=255)
                                                                 tAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTc                                                                                      >  1:584755/1‑150 (MQ=255)
                                                                 tAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTc                                                                                      <  1:453639/150‑1 (MQ=255)
                                                                 tAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTc                                                                                      >  2:462872/1‑150 (MQ=255)
                                                                  aaaaGAAAGCTGGGTAGAAACAGGCGGCAGTACGCCTTTGGTTTATCAATTTTATACAATACATGTAAAAAAAGGGCACTGAAATTCAGGACCATTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCTACGATGTCACCAGTCt                                                                                     >  1:1107619/1‑150 (MQ=255)
                                                                  aaaaGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTTGTTTAACCATTTTATACAATCCAGGTAAAAAAACGGCCCGGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGTTATTCGACGATGTCACCAGGCt                                                                                     <  2:698266/150‑1 (MQ=255)
                                                                  aaaaGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCt                                                                                     >  2:313652/1‑150 (MQ=255)
                                                                  aaaaGAAAGCTCGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCAATGTAAAAAAAGGGCCCTGAAATTCAGGAACCTTTCTCGCATCAGCCTTTAATCTGTATCACCAGATATTCGACGATGACAAAAGTCt                                                                                     >  2:1070692/1‑150 (MQ=255)
                                                                   aaagaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCtt                                                                                    <  2:324912/150‑1 (MQ=255)
                                                                      gaaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGCTGTCACAAGTCttaat                                                                                 >  1:854320/1‑150 (MQ=255)
                                                                       aaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAATGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaatt                                                                                <  1:1171248/150‑1 (MQ=255)
                                                                       aaagCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaatt                                                                                <  1:361107/150‑1 (MQ=255)
                                                                         agCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCttaattaa                                                                              >  2:429881/1‑150 (MQ=255)
                                                                            tGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTg                                                                           >  2:1202897/1‑150 (MQ=255)
                                                                             ggggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGCCCCTTTATGGCATCAGCCTTTAATCTGTTTCACAAGATATTCGACGATGTCACAATTCTTAATTAACTGt                                                                          >  2:861537/1‑150 (MQ=255)
                                                                              gggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGCCCCTTTCTGGCATCAGCCTTAAATCTGTTTCACAAGATATTCGAAGATTTCACCAGTCTAAATTAAATGtt                                                                         >  2:1137600/1‑150 (MQ=255)
                                                                              gggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGtt                                                                         >  1:1193616/1‑150 (MQ=255)
                                                                               ggAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTAATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGttt                                                                        <  1:1250887/150‑1 (MQ=255)
                                                                                gagaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTtc                                                                       >  2:224189/1‑150 (MQ=255)
                                                                                 agaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcgac                                                                                                   >  1:58576/1‑121 (MQ=255)
                                                                                 agaAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTcgac                                                                                                   <  2:58576/121‑1 (MQ=255)
                                                                                    aaCATGCGGAAGTACGCCTTTGGTTAATCCATTATATACTATCCAAGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGGCTTTAATCTGTTTCACCAGATATTCGACGATTTCACCAGTCTTACTTAACTGTTTCTCGc                                                                   <  1:131511/150‑1 (MQ=255)
                                                                                      cAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATATAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCg                                                                 >  1:255032/1‑150 (MQ=255)
                                                                                      cAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACTATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTGTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCg                                                                 <  2:218418/150‑1 (MQ=255)
                                                                                        ggtggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGtt                                                               >  2:1016975/4‑150 (MQ=255)
                                                                                           ggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGcg                                                            <  2:375040/150‑1 (MQ=255)
                                                                                           ggcAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCGTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTCCTCGCCGTTGcg                                                            <  2:798256/150‑1 (MQ=255)
                                                                                                tACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGat                                                       <  1:437450/150‑1 (MQ=255)
                                                                                                 aCGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGata                                                      <  1:1290476/150‑1 (MQ=255)
                                                                                                  cGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAGGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGatat                                                     >  2:586943/1‑150 (MQ=255)
                                                                                                    ccTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATAttt                                                   >  1:783934/1‑150 (MQ=255)
                                                                                                    ccTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGGCTTAATTAACTGTTTCTCGCCGTTGCGACGATAttt                                                   <  2:176336/150‑1 (MQ=255)
                                                                                                      tttGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTtat                                                 <  1:1182967/150‑1 (MQ=255)
                                                                                                      tttGGTTTATCCATTTTATAAAATCCATTTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATAATCGACGATGTCACCAGTCTTAATTAACTGTTTCACGCCGTTGCGACGATATTtat                                                 <  1:1043725/150‑1 (MQ=255)
                                                                                                          gTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTc                                             >  1:369661/1‑150 (MQ=255)
                                                                                                             tATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCGGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGat                                          >  2:31585/1‑150 (MQ=255)
                                                                                                             tATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGat                                          >  2:1020401/1‑150 (MQ=255)
                                                                                                              aTCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGata                                         <  1:554914/150‑1 (MQ=255)
                                                                                                                ccATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGCACCCTTTCTTGCATAAGCCTTTAATCTCTTTCACCAGATATTAGACGATGTCAACAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATAtc                                       >  2:768987/1‑150 (MQ=255)
                                                                                                                      tataCAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGtt                                 >  1:1148711/1‑150 (MQ=255)
                                                                                                                          cAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCg                             >  2:400297/1‑150 (MQ=255)
                                                                                                                          cAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCg                             >  2:956155/1‑150 (MQ=255)
                                                                                                                          cAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGGCGTTGTCg                             <  1:382536/150‑1 (MQ=255)
                                                                                                                           aaTCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGa                            >  1:1017662/1‑150 (MQ=255)
                                                                                                                            aTCCATGTAAAAAAATGGCCCTGAAATTCATGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAg                           <  1:712934/150‑1 (MQ=255)
                                                                                                                                aTGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTa                       >  1:533861/1‑150 (MQ=255)
                                                                                                                                 tGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTAc                      <  1:222652/150‑1 (MQ=255)
                                                                                                                                  gTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACg                     >  2:24209/1‑150 (MQ=255)
                                                                                                                                    aaaaaaaGGGCCCTGAAATTCAGGACCCGTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAAGTAACTGTTTCTCGCCGTTGCGCCGAGATTTATATTCGATATCGTCGTTGTCGAGGTTACGGt                   >  2:843264/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGGGCCCTGAAATTCAGGACCCTTTATGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCCATATCGTCGTTGTCGAGGTTACGGTc                  >  2:173913/1‑150 (MQ=255)
                                                                                                                                      aaaaaGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCg                 <  1:720341/150‑1 (MQ=255)
                                                                                                                                        aacgggCCCTGAAATGCAGGCCCCTTTCTGGCATCAGCCTTTAATCTGTGTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCAGTTGCGACGATATTTATATTAGATATCGTCGTTTTCGAGGTTACGGTCGcc               >  1:111284/4‑150 (MQ=255)
                                                                                                                                          aGGGTCCTGAATTTCAGGGCCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCa             >  2:1241979/1‑150 (MQ=255)
                                                                                                                                           gggCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAg            <  1:224189/150‑1 (MQ=255)
                                                                                                                                           gggCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTAACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACTATATTTATATTAGATATCGTCGTTGTCGAGGTTACGGTCGCACAg            >  1:805907/1‑150 (MQ=255)
                                                                                                                                            ggtccagAATTTCAGGGCCCTTTCTGGCATCAGACTTTAATCTGTTTCACCAGATATACGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCAGTTGCGACGATATTTATATTAGATATCGACGTTGTCGAGGTTACGGTCGCCCAGc           >  2:430566/7‑150 (MQ=255)
                                                                                                                                             gCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGca          >  1:308809/1‑150 (MQ=255)
                                                                                                                                                  gAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATa     >  1:561259/1‑150 (MQ=255)
                                                                                                                                                   aaaTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAg    >  1:98971/1‑150 (MQ=255)
                                                                                                                                                   aaaTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAg    <  1:289540/150‑1 (MQ=255)
                                                                                                                                                     aTTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAgtg  <  1:92940/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGTACAAGCCGAAAGTAAAAGAAAGCTGGGGAGAAACAGGCGGCAGTACGCCTTTGGTTTATCCATTTTATACAATCCATGTAAAAAAAGGGCCCTGAAATTCAGGACCCTTTCTGGCATCAGCCTTTAATCTGTTTCACCAGATATTCGACGATGTCACCAGTCTTAATTAACTGTTTCTCGCCGTTGCGACGATATTTATATTCGATATCGTCGTTGTCGAGGTTACGGTCGCCCAGCACAATAGTG  >  NC_000913/216888‑217186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: