Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,927,123 A→T 6.3% intergenic (‑120/‑32) asnC ← / → asnA transcriptional activator of asnA; autorepressor/asparagine synthetase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,927,1230AT 6.3% 299.7 / 8.0 107intergenic (‑120/‑32)asnC/asnAtranscriptional activator of asnA; autorepressor/asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base A (43/57);  new base T (4/3);  total (47/60)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

Only 100 of 109 total aligned reads displayed.

GTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCC  >  NC_000913/3926974‑3927269
                                                                                                                                                     |                                                                                                                                                  
gTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTaa                                                                                                                                                    >  2:392446/1‑150 (MQ=255)
gTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTaa                                                                                                                                                    <  1:435176/150‑1 (MQ=255)
 tCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAAt                                                                                                                                                   <  2:1329597/150‑1 (MQ=255)
 gccAGAGTGTCGATCAGATAATTTTACATATGATTTTCTTATGAGTTTTGAGGTTTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGGTAGGCTTTTTATTGAATGATTATTGCATCTGTGGCGGTTTTTGTTGCTTAAt                                                                                                                                                   <  2:539438/149‑1 (MQ=255)
  ccAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATc                                                                                                                                                  >  1:23480/1‑150 (MQ=255)
   cAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCa                                                                                                                                                 >  1:1167594/1‑150 (MQ=255)
   cAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCa                                                                                                                                                 <  1:483142/150‑1 (MQ=255)
    aGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTTAATGATTATTGCATGTGTGTCTTTTTTTTTTGCTTAATCAt                                                                                                                                                >  1:225788/1‑150 (MQ=255)
     taTTGTCTATCAGATAATTTTCCATATGATTTTCTTATGCGGATTGATGAATCATTCTATTTTAGCCGTCTTTTTTAATGAATCAAAAGTGATTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTGGCTTAATCATa                                                                                                                                               <  2:764098/149‑1 (MQ=255)
      aTTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATaa                                                                                                                                              >  1:1324706/1‑150 (MQ=255)
       ttGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGc                                                                                                                                            <  2:543426/150‑1 (MQ=255)
       ttGTAGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAg                                                                                                                                             <  2:463715/150‑1 (MQ=255)
        tGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGc                                                                                                                                            <  1:1154326/150‑1 (MQ=255)
           cGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAAc                                                                                                                                         <  2:927841/150‑1 (MQ=255)
            gATCTGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATGATTAAGCAACa                                                                                                                                        <  2:257483/150‑1 (MQ=255)
            gATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACa                                                                                                                                        >  2:771782/1‑150 (MQ=255)
            gATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGGTTTTGTTGCTTATGATTAAGCAACa                                                                                                                                        <  1:769780/150‑1 (MQ=255)
               cAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGa                                                                                                                                     <  1:562774/150‑1 (MQ=255)
                aGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTTCATGTTTGTCTGTTTTTGTAGCTTAATCATAAGCAACAGGAc                                                                                                                                    >  2:739302/1‑150 (MQ=255)
                 gATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATGATTAAGCAACaaaaac                                                                                                                                   <  1:1048153/150‑6 (MQ=255)
                 gATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACg                                                                                                                                   <  2:938742/150‑1 (MQ=255)
                  aTAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTAAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATAATAAGCACCAGGACGc                                                                                                                                  >  2:255170/1‑150 (MQ=255)
                     aTTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAgg                                                                                                                               >  1:95779/1‑150 (MQ=255)
                     aTTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCCTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAgg                                                                                                                               <  2:809522/150‑1 (MQ=255)
                           cATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGtata                                                                                                                         >  2:909548/1‑150 (MQ=255)
                           cATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGtata                                                                                                                         >  2:150151/1‑150 (MQ=255)
                                 aTTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAAt                                                                                                                   <  1:1019452/150‑1 (MQ=255)
                                  ttttCTTATGCGGATTGATGATTCATTCTATTTTTGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATg                                                                                                                  <  1:48200/150‑1 (MQ=255)
                                     tCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGaaa                                                                                                               <  1:142706/150‑1 (MQ=255)
                                      cTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGaaaa                                                                                                              >  2:865121/1‑150 (MQ=255)
                                        tATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAAcc                                                                                                            <  1:425584/150‑1 (MQ=255)
                                           gCGGATTTTTGATTCATTCTAATTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGAATGTGTGTCTGTGTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACAGCt                                                                                                         <  2:963365/150‑1 (MQ=255)
                                            cGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCtt                                                                                                        <  1:780142/150‑1 (MQ=255)
                                             ggATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTa                                                                                                       <  2:1293414/150‑1 (MQ=255)
                                                        tcattcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTGTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaac                                                                                            <  1:1229711/150‑1 (MQ=255)
                                                         cattcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaaca                                                                                           <  2:653748/150‑1 (MQ=255)
                                                         cattcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaaca                                                                                           <  2:326406/150‑1 (MQ=255)
                                                            tcTATTTTAGCCTTCTTTTTTAATGATTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                        <  2:461649/150‑1 (MQ=255)
                                                            tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTATGCTTTTTATTGAATGATTATTGCATGTGTGTCGTTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                        <  2:494122/150‑1 (MQ=255)
                                                            tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaacaact                                                                                        >  1:1177395/1‑149 (MQ=255)
                                                            tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                        >  1:1170238/1‑150 (MQ=255)
                                                            tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                        <  2:311866/150‑1 (MQ=255)
                                                            tcTATTTTAGCCTTCTTTATTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTCTTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                        <  2:304830/150‑1 (MQ=255)
                                                             cTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGt                                                                                       <  1:1020359/150‑1 (MQ=255)
                                                               atattAGCCTTCTTTTTAAATGAATCAAAAGTGAGTTAGGCTATTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCa                                                                                     <  2:1054368/147‑1 (MQ=255)
                                                                   tAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAAtt                                                                                 >  2:680888/1‑150 (MQ=255)
                                                                    aGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTa                                                                                <  1:734194/150‑1 (MQ=255)
                                                                     gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTTTTGCATGTGTGTCGGTTTTTGTTGATTAATCATAAGCAACAGGACGAAGGAGTATAAAAAATGATAACCGCTTACATTGCAAAACAACGTCAATTTAg                                                                               >  2:11797/1‑150 (MQ=255)
                                                                     gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAg                                                                               <  1:260728/150‑1 (MQ=255)
                                                                       cttcttTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACATTACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCt                                                                             >  1:154884/1‑150 (MQ=255)
                                                                            tttttAATGAATCAAAAGTGAGTTATGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGTAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTg                                                                        <  2:335212/150‑1 (MQ=255)
                                                                            tttttAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTg                                                                        <  1:301921/150‑1 (MQ=255)
                                                                                   tGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAAtctc                                                                 >  1:275257/1‑150 (MQ=255)
                                                                                    gAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAAtctcc                                                                >  2:194540/1‑149 (MQ=255)
                                                                                    gAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGACAAACAAAGTCAAATTAGCTTCGTGAAATATCa                                                                >  1:825054/1‑150 (MQ=255)
                                                                                      aTCAAAAGTGAGTTAGTCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACt                                                              <  2:1029049/150‑1 (MQ=255)
                                                                                      aTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATTCATAAGCAACAGGACGCACGAGTATAAAAAATTAAAACCGCTTACATTGCCAAACAACTTCAAATTAGCTTCGTCAAATCTCaat                                                              >  2:344149/1‑148 (MQ=255)
                                                                                      aTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACTTCAAATTATCTTAGTGAAATCACACt                                                              >  1:528790/1‑150 (MQ=255)
                                                                                        cAAAAGTTAGTTAGTCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACttt                                                            <  1:75457/150‑1 (MQ=255)
                                                                                         aaaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGCCAAATTAGCTTCGTGAAATCTCACtttt                                                           >  1:468742/1‑150 (MQ=255)
                                                                                         aaaaGTGAGTTAGGATTTTTATTGAATGATTATTGAATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAAAAGGAAGCAGGAGTATAAAAAATGATAACCGCTTACATTGCGAAAAAACGGCAAATTAGCTTCGTGAAATCTCACtttt                                                           >  2:569702/1‑150 (MQ=255)
                                                                                          aaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACGGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTtc                                                          <  2:467543/150‑1 (MQ=255)
                                                                                          aaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACGGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTtc                                                          <  2:1034818/150‑1 (MQ=255)
                                                                                             gTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCg                                                       <  1:745279/150‑1 (MQ=255)
                                                                                             gTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCg                                                       <  2:1026228/150‑1 (MQ=255)
                                                                                               gAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTc                                                     <  2:275257/150‑1 (MQ=255)
                                                                                                aGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCa                                                    >  1:895185/1‑150 (MQ=255)
                                                                                                 gTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAAACGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCaa                                                   >  2:849875/1‑150 (MQ=255)
                                                                                                 gTTAGGCTTTTTATTGAATGATTATTGCATCTGTGTCGGTTTTTGTTGCTTAATCATAAGAAACAGGACGCAGGAGTTTAAAAAATGAAAACCGCTTACATTGCCAAACATCGTCAATTTAGCTTCGTGAAATCTCACTTTTCTCGTCaa                                                   >  2:909137/1‑150 (MQ=255)
                                                                                                  ttAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTCCTTAATCATCAGCAACAGGACGCAGGAGTATTAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAAc                                                  >  2:819003/1‑150 (MQ=255)
                                                                                                   tAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACt                                                 <  1:1307268/150‑1 (MQ=255)
                                                                                                     ggCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTgg                                               >  1:957114/1‑150 (MQ=255)
                                                                                                     ggCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTgg                                               <  2:361989/150‑1 (MQ=255)
                                                                                                          tttATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGgaagaa                                          <  1:830711/150‑1 (MQ=255)
                                                                                                           ttATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAc                                         >  2:417928/1‑150 (MQ=255)
                                                                                                            tATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCTCTTACATTGCAAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAca                                        >  1:37941/1‑149 (MQ=255)
                                                                                                             aTTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGACAAATTAGCTTCGTGAAATCTCCCTTTTCTCGTCAACTGGAAGAACGt                                       >  2:180789/1‑150 (MQ=255)
                                                                                                                gAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTg                                    <  1:392446/150‑1 (MQ=255)
                                                                                                                        attGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGAt                            <  2:225788/150‑1 (MQ=255)
                                                                                                                         ttGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATc                           <  2:196533/150‑1 (MQ=255)
                                                                                                                           gCATGTGTGTCGGTTTTAGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGa                         <  1:1259250/150‑1 (MQ=255)
                                                                                                                                   gtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACACCGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGTCTGATCGAAGTCCAgg                 <  1:1336658/150‑1 (MQ=255)
                                                                                                                                   gtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCCgg                 >  1:201634/1‑150 (MQ=255)
                                                                                                                                   gtCGGTGTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAgg                 <  2:413039/150‑1 (MQ=255)
                                                                                                                                      ggTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGgcgc              >  1:359067/1‑150 (MQ=255)
                                                                                                                                       gtgtttGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGcc             <  2:711062/147‑1 (MQ=255)
                                                                                                                                       gTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGcc             <  2:701816/150‑1 (MQ=255)
                                                                                                                                          tttGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTAACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGAt          >  1:574210/1‑150 (MQ=255)
                                                                                                                                           ctgttgCTTATGATTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGAtt         >  1:240622/2‑150 (MQ=255)
                                                                                                                                           ctgttgCTTATGATTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAAGTCTGGGGCTGATCGAAGTCCAGGCGCCGAtt         >  1:73980/2‑150 (MQ=255)
                                                                                                                                            tgttgCTTATGATTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTc        >  1:1255109/1‑150 (MQ=255)
                                                                                                                                            tgttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCACCTGGAAGCACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTc        >  1:1238529/1‑150 (MQ=255)
                                                                                                                                             gttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCt       >  1:158491/1‑150 (MQ=255)
                                                                                                                                             gttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCt       >  2:361298/1‑150 (MQ=255)
                                                                                                                                             gttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCt       >  1:1272481/1‑150 (MQ=255)
                                                                                                                                             gttgCTAAATCATAAGCAACAGGACGCAGGAGAATAAAAAATGAAAACCGCTTACATTGCAAAACAACGTCAAATTAGCTTCGGGAAAACTCACATTTCTCGTCAACTTGAAGAAAGTCTCGGGCTGATCGAAGTACAGGCGACTATTCt       >  2:801907/1‑150 (MQ=255)
                                                                                                                                              ttgCTTAATCATAAGCAACAGGACGCAGTAGTATAAAAATTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCTTCATCTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCtt      <  1:961057/150‑1 (MQ=255)
                                                                                                                                                 cTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGc   >  2:574331/1‑150 (MQ=255)
                                                                                                                                                 cTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACCTCTGGGGCTGATCGAAGTCCAGGCGACGATTCTTAGc   >  1:129151/1‑150 (MQ=255)
                                                                                                                                                  ttAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGcc  <  2:1275735/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCC  >  NC_000913/3926974‑3927269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: