Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,927,126 A→T 5.4% intergenic (‑123/‑29) asnC ← / → asnA transcriptional activator of asnA; autorepressor/asparagine synthetase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,927,1260AT 5.4% 308.3 / 3.9 104intergenic (‑123/‑29)asnC/asnAtranscriptional activator of asnA; autorepressor/asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base A (43/55);  new base T (3/3);  total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGG  >  NC_000913/3926977‑3927275
                                                                                                                                                     |                                                                                                                                                     
cAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCa                                                                                                                                                       >  1:1167594/1‑150 (MQ=255)
cAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCa                                                                                                                                                       <  1:483142/150‑1 (MQ=255)
 aGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTTAATGATTATTGCATGTGTGTCTTTTTTTTTTGCTTAATCAt                                                                                                                                                      >  1:225788/1‑150 (MQ=255)
  taTTGTCTATCAGATAATTTTCCATATGATTTTCTTATGCGGATTGATGAATCATTCTATTTTAGCCGTCTTTTTTAATGAATCAAAAGTGATTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTGGCTTAATCATa                                                                                                                                                     <  2:764098/149‑1 (MQ=255)
    ttGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGc                                                                                                                                                  <  2:543426/150‑1 (MQ=255)
    ttGTAGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAg                                                                                                                                                   <  2:463715/150‑1 (MQ=255)
     tGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGc                                                                                                                                                  <  1:1154326/150‑1 (MQ=255)
        cGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAAc                                                                                                                                               <  2:927841/150‑1 (MQ=255)
        cGATCAGATAATTTTCCATAGGATTTTCTTATGCGGAGTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGATTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAAc                                                                                                                                               <  2:770260/150‑1 (MQ=255)
         gATCTGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATGATTAAGCAACa                                                                                                                                              <  2:257483/150‑1 (MQ=255)
         gATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACa                                                                                                                                              >  2:771782/1‑150 (MQ=255)
            cAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGa                                                                                                                                           <  1:562774/150‑1 (MQ=255)
             aGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTTCATGTTTGTCTGTTTTTGTAGCTTAATCATAAGCAACAGGAc                                                                                                                                          >  2:739302/1‑150 (MQ=255)
              gATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATGATTAAGCAACaaaaac                                                                                                                                         <  1:1048153/150‑6 (MQ=255)
              gATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACg                                                                                                                                         <  2:938742/150‑1 (MQ=255)
               aTAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTAAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATAATAAGCACCAGGACGc                                                                                                                                        >  2:255170/1‑150 (MQ=255)
                  aTTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAgg                                                                                                                                     >  1:95779/1‑150 (MQ=255)
                  aTTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCCTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAgg                                                                                                                                     <  2:809522/150‑1 (MQ=255)
                        cATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGtata                                                                                                                               >  2:909548/1‑150 (MQ=255)
                        cATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGtata                                                                                                                               >  2:150151/1‑150 (MQ=255)
                              aTTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAAt                                                                                                                         <  1:1019452/150‑1 (MQ=255)
                               ttttCTTATGCGGATTGATGATTCATTCTATTTTTGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATg                                                                                                                        <  1:48200/150‑1 (MQ=255)
                                  tCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGaaa                                                                                                                     <  1:142706/150‑1 (MQ=255)
                                   cTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGaaaa                                                                                                                    >  2:865121/1‑150 (MQ=255)
                                     tATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAAcc                                                                                                                  <  1:425584/150‑1 (MQ=255)
                                        gCGGATTTTTGATTCATTCTAATTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGAATGTGTGTCTGTGTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACAGCt                                                                                                               <  2:963365/150‑1 (MQ=255)
                                         cGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCtt                                                                                                              <  1:780142/150‑1 (MQ=255)
                                          ggATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTa                                                                                                             <  2:1293414/150‑1 (MQ=255)
                                                     tcattcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTGTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaac                                                                                                  <  1:1229711/150‑1 (MQ=255)
                                                      cattcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaaca                                                                                                 <  2:326406/150‑1 (MQ=255)
                                                      cattcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaaca                                                                                                 <  2:653748/150‑1 (MQ=255)
                                                         tcTATTTTTGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGATTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                              <  1:249001/150‑1 (MQ=255)
                                                         tcTATTTTAGCCTTCTTTTTTAATGATTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                              <  2:461649/150‑1 (MQ=255)
                                                         tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTATGCTTTTTATTGAATGATTATTGCATGTGTGTCGTTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                              <  2:494122/150‑1 (MQ=255)
                                                         tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAaacaact                                                                                              >  1:1177395/1‑149 (MQ=255)
                                                         tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                              <  2:311866/150‑1 (MQ=255)
                                                         tcTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                              >  1:1170238/1‑150 (MQ=255)
                                                         tcTATTTTAGCCTTCTTTATTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTCTTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACg                                                                                              <  2:304830/150‑1 (MQ=255)
                                                          cTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGt                                                                                             <  1:1020359/150‑1 (MQ=255)
                                                            atattAGCCTTCTTTTTAAATGAATCAAAAGTGAGTTAGGCTATTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCa                                                                                           <  2:1054368/147‑1 (MQ=255)
                                                                tAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAAtt                                                                                       >  2:680888/1‑150 (MQ=255)
                                                                 aGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTa                                                                                      <  1:734194/150‑1 (MQ=255)
                                                                  gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTTTTGCATGTGTGTCGGTTTTTGTTGATTAATCATAAGCAACAGGACGAAGGAGTATAAAAAATGATAACCGCTTACATTGCAAAACAACGTCAATTTAg                                                                                     >  2:11797/1‑150 (MQ=255)
                                                                  gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAg                                                                                     <  1:260728/150‑1 (MQ=255)
                                                                  gCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAg                                                                                     <  1:842929/150‑1 (MQ=255)
                                                                    cttcttTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACATTACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCt                                                                                   >  1:154884/1‑150 (MQ=255)
                                                                     ttcttTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCtt                                                                                  >  2:48875/1‑150 (MQ=255)
                                                                         tttttAATGAATCAAAAGTGAGTTATGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGTAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTg                                                                              <  2:335212/150‑1 (MQ=255)
                                                                         tttttAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTg                                                                              <  1:301921/150‑1 (MQ=255)
                                                                                tGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAAtctc                                                                       >  1:275257/1‑150 (MQ=255)
                                                                                 gAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAAtctcc                                                                      >  2:194540/1‑149 (MQ=255)
                                                                                 gAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGACAAACAAAGTCAAATTAGCTTCGTGAAATATCa                                                                      >  1:825054/1‑150 (MQ=255)
                                                                                   aTCAAAAGTGAGTTAGTCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACt                                                                    <  2:1029049/150‑1 (MQ=255)
                                                                                   aTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTATTCATAAGCAACAGGACGCACGAGTATAAAAAATTAAAACCGCTTACATTGCCAAACAACTTCAAATTAGCTTCGTCAAATCTCaat                                                                    >  2:344149/1‑148 (MQ=255)
                                                                                   aTCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACTTCAAATTATCTTAGTGAAATCACACt                                                                    >  1:528790/1‑150 (MQ=255)
                                                                                     cAAAAGTTAGTTAGTCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACttt                                                                  <  1:75457/150‑1 (MQ=255)
                                                                                      aaaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGCCAAATTAGCTTCGTGAAATCTCACtttt                                                                 >  1:468742/1‑150 (MQ=255)
                                                                                      aaaaGTGAGTTAGGATTTTTATTGAATGATTATTGAATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAAAAGGAAGCAGGAGTATAAAAAATGATAACCGCTTACATTGCGAAAAAACGGCAAATTAGCTTCGTGAAATCTCACtttt                                                                 >  2:569702/1‑150 (MQ=255)
                                                                                       aaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACGGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTtc                                                                <  2:467543/150‑1 (MQ=255)
                                                                                       aaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACGGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTtc                                                                <  2:1034818/150‑1 (MQ=255)
                                                                                       aaaGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTtc                                                                >  1:1289615/1‑150 (MQ=255)
                                                                                          gTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCg                                                             <  2:1026228/150‑1 (MQ=255)
                                                                                          gTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCg                                                             <  1:745279/150‑1 (MQ=255)
                                                                                           tGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAAAGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGt                                                            >  2:323384/1‑150 (MQ=255)
                                                                                            gAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTc                                                           <  2:275257/150‑1 (MQ=255)
                                                                                             aGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCa                                                          >  1:895185/1‑150 (MQ=255)
                                                                                              gTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAAACGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCaa                                                         >  2:849875/1‑150 (MQ=255)
                                                                                              gTTAGGCTTTTTATTGAATGATTATTGCATCTGTGTCGGTTTTTGTTGCTTAATCATAAGAAACAGGACGCAGGAGTTTAAAAAATGAAAACCGCTTACATTGCCAAACATCGTCAATTTAGCTTCGTGAAATCTCACTTTTCTCGTCaa                                                         >  2:909137/1‑150 (MQ=255)
                                                                                               ttAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTCCTTAATCATCAGCAACAGGACGCAGGAGTATTAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAAc                                                        >  2:819003/1‑150 (MQ=255)
                                                                                                tAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACt                                                       <  1:1307268/150‑1 (MQ=255)
                                                                                                  ggCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTgg                                                     >  1:957114/1‑150 (MQ=255)
                                                                                                  ggCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTgg                                                     <  2:361989/150‑1 (MQ=255)
                                                                                                       tttATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGgaagaa                                                <  1:830711/150‑1 (MQ=255)
                                                                                                        ttATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAc                                               >  2:417928/1‑150 (MQ=255)
                                                                                                         tATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCTCTTACATTGCAAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAca                                              >  1:37941/1‑149 (MQ=255)
                                                                                                          aTTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGACAAATTAGCTTCGTGAAATCTCCCTTTTCTCGTCAACTGGAAGAACGt                                             >  2:180789/1‑150 (MQ=255)
                                                                                                                tGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTgggg                                       <  1:509454/150‑1 (MQ=255)
                                                                                                                     attGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGAt                                  <  2:225788/150‑1 (MQ=255)
                                                                                                                      ttGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATc                                 <  2:196533/150‑1 (MQ=255)
                                                                                                                        gCATGTGTGTCGGTTTTAGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGa                               <  1:1259250/150‑1 (MQ=255)
                                                                                                                                gtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACACCGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGTCTGATCGAAGTCCAgg                       <  1:1336658/150‑1 (MQ=255)
                                                                                                                                gtCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCCgg                       >  1:201634/1‑150 (MQ=255)
                                                                                                                                gtCGGTGTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAgg                       <  2:413039/150‑1 (MQ=255)
                                                                                                                                   ggTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGgcgc                    >  1:359067/1‑150 (MQ=255)
                                                                                                                                    gTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGcc                   <  2:701816/150‑1 (MQ=255)
                                                                                                                                       tttGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTAACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGAt                >  1:574210/1‑150 (MQ=255)
                                                                                                                                        ctgttgCTTATGATTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGAtt               >  1:240622/2‑150 (MQ=255)
                                                                                                                                        ctgttgCTTATGATTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAAAGTCTGGGGCTGATCGAAGTCCAGGCGCCGAtt               >  1:73980/2‑150 (MQ=255)
                                                                                                                                         tgttgCTTATGATTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTc              >  1:1255109/1‑150 (MQ=255)
                                                                                                                                         tgttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCACCTGGAAGCACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTc              >  1:1238529/1‑150 (MQ=255)
                                                                                                                                          gttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCt             >  1:158491/1‑150 (MQ=255)
                                                                                                                                          gttgCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCt             >  1:1272481/1‑150 (MQ=255)
                                                                                                                                          gttgCTAAATCATAAGCAACAGGACGCAGGAGAATAAAAAATGAAAACCGCTTACATTGCAAAACAACGTCAAATTAGCTTCGGGAAAACTCACATTTCTCGTCAACTTGAAGAAAGTCTCGGGCTGATCGAAGTACAGGCGACTATTCt             >  2:801907/1‑150 (MQ=255)
                                                                                                                                           ttgCTTAATCATAAGCAACAGGACGCAGTAGTATAAAAATTGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCTTCATCTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCtt            <  1:961057/150‑1 (MQ=255)
                                                                                                                                              cTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGc         >  2:574331/1‑150 (MQ=255)
                                                                                                                                              cTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACCTCTGGGGCTGATCGAAGTCCAGGCGACGATTCTTAGc         >  1:129151/1‑150 (MQ=255)
                                                                                                                                               ttAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGcc        <  2:1275735/150‑1 (MQ=255)
                                                                                                                                                   tCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCgtgt    <  1:417928/150‑1 (MQ=255)
                                                                                                                                                   tCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTACAGCCGCCGATTCTTAGCCgtgt    >  1:195859/1‑150 (MQ=255)
                                                                                                                                                     aTAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCAGATTCTTAGCCGTGTgg  >  1:710004/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCGTGAAATCTCACTTTTCTCGTCAACTGGAAGAACGTCTGGGGCTGATCGAAGTCCAGGCGCCGATTCTTAGCCGTGTGG  >  NC_000913/3926977‑3927275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: