Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,117,473	A→G	5.5%	R206R (CGT→CGC)	glpF ←	glycerol facilitator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,117,473	0	A	G	5.5%	192.8 / 3.1	73	R206R (CGT→CGC)	glpF	glycerol facilitator
Reads supporting (aligned to +/- strand):  ref base A (33/35);  new base G (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

TCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAT  >  NC_000913/4117328‑4117619                                                                                                                                                  |                                                                                                                                                   tCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCAcgcg                                                                                                                                                <  1:993542/150‑1 (MQ=255) tCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCAcgcg                                                                                                                                                <  1:366105/150‑1 (MQ=255)   aGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCAAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACgcgcg                                                                                                                                              >  1:1115610/1‑149 (MQ=255)      tttcGGTAGTCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTggg                                                                                                                                           <  2:1030756/147‑1 (MQ=255)         cGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTc                                                                                                                                        <  2:688325/150‑1 (MQ=255)           gTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGCCTTTCGGCCCGAAGTCGCGCGCTGGGTTCAt                                                                                                                                      >  1:1280617/1‑150 (MQ=255)           gTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCGCGCGCTGGGTTCAt                                                                                                                                      >  1:1285952/1‑150 (MQ=255)             aGGCAAATGCACCTACAATCGCGCAAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGGCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATgg                                                                                                                                    <  1:1344655/150‑1 (MQ=255)              ggCAAATGCACCTACAATCGCGCCAACGATAGCGCCGAAAAGCGGCACCAGCAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCCGCCCGCCAGCCAGGCAAAGACTTTCGGACAGAAGTCACGCGCTGGGTTCATgga                                                                                                                                   >  2:78731/1‑149 (MQ=255)               gCAAATGCACCTACACTCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGCCTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCa                                                                                                                                  >  1:742146/1‑150 (MQ=255)                    tGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAAcc                                                                                                                             <  1:1013240/150‑1 (MQ=255)                    tGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAAcc                                                                                                                             <  1:1206071/150‑1 (MQ=255)                     gCACCTACAATCGCGACAACCATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGCTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCt                                                                                                                            >  2:853244/1‑150 (MQ=255)                       aCCTACAATCGCGCCACCGTTAGGGCCGAAAAGCGGCACCAGGAAGTCAGGAATGGCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGt                                                                                                                          <  1:250021/150‑1 (MQ=255)                        ccTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTc                                                                                                                         <  1:147616/150‑1 (MQ=255)                        ccTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTc                                                                                                                         >  2:371333/1‑150 (MQ=255)                        ccTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTc                                                                                                                         >  1:1134771/1‑150 (MQ=255)                        ccTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTc                                                                                                                         >  2:804680/1‑150 (MQ=255)                               tCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGc                                                                                                                  <  1:664383/150‑1 (MQ=255)                                cgcgCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGcc                                                                                                                 <  2:1040053/150‑1 (MQ=255)                                    ccAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATa                                                                                                             <  2:852294/150‑1 (MQ=255)                                      aaCGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGa                                                                                                           >  1:1341360/1‑150 (MQ=255)                                           tAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAAAGGGCCCATAGATgcgc                                                                                                      >  2:511649/1‑150 (MQ=255)                                             gggCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAACCCTGTCAATGGGCCCATAGATGCGCCa                                                                                                    >  2:1092165/1‑150 (MQ=255)                                             gggCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCa                                                                                                    >  2:701800/1‑150 (MQ=255)                                              ggCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCaa                                                                                                   >  1:912675/1‑150 (MQ=255)                                               gCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCCCGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAAt                                                                                                  >  1:1173005/1‑150 (MQ=255)                                               gCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAAt                                                                                                  >  2:1102160/1‑150 (MQ=255)                                                  gAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTAACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCAAAGATGCGCCAATGAc                                                                                               >  2:255016/1‑150 (MQ=255)                                                   aaaaGCGGCACCAGGCAGTAAGGAAGGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTATCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGAcc                                                                                              <  2:976200/150‑1 (MQ=255)                                                    aaaGCGGCACCAGGTAGTAAGGAATGTCTCAGCCGCCTGTAAAGGCGACATTGCCCCAGCCCGCCTGCCAGGCAAAGTCTTTCGGACCGAAGTCACGCGCTGGGCTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCg                                                                                             <  2:682078/150‑1 (MQ=255)                                                    aaaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCg                                                                                             <  2:466683/150‑1 (MQ=255)                                                     aaGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGc                                                                                            <  2:995813/150‑1 (MQ=255)                                                         ggCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc                                                                                        <  1:633508/150‑1 (MQ=255)                                                         ggCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc                                                                                        >  2:772689/1‑150 (MQ=255)                                                            aCCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGt                                                                                     <  1:1346866/150‑1 (MQ=255)                                                             ccAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTa                                                                                    <  1:888359/150‑1 (MQ=255)                                                              cAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACATTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGCCCGCAATCAGTAg                                                                                   >  1:129686/1‑150 (MQ=255)                                                                 gAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAcc                                                                                <  2:385279/150‑1 (MQ=255)                                                                  aaGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCACAATCAGTAGACCa                                                                               <  2:1173021/150‑1 (MQ=255)                                                                    gTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAACACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAAt                                                                             >  2:1050158/1‑150 (MQ=255)                                                                             gTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCACAATCAGTAGACCAATCAGCAAggg                                                                    <  2:1115610/150‑1 (MQ=255)                                                                                tctGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGc                                                                 <  1:232105/150‑1 (MQ=255)                                                                                      gccgGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGCCCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAgg                                                           >  1:378266/1‑150 (MQ=255)                                                                                      gccgGTAAACGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCACTGGGCCCATAGATGCTCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAgg                                                           <  2:874653/150‑1 (MQ=255)                                                                                               ggCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGCCCGCAATCAGTAGCCCAATCAGCAAGGGAGCCAAAGGGCGGCGTgg                                                  >  2:638312/1‑150 (MQ=255)                                                                                               ggCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTgg                                                  >  1:1296727/1‑150 (MQ=255)                                                                                               ggCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTgg                                                  >  1:1204426/1‑150 (MQ=255)                                                                                                        gCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACAACGtt                                         >  1:897003/1‑150 (MQ=255)                                                                                                        accccAGCCCGCCAGCCAGGCAAAGACTTTCAGAACGAAATCACGCGCTGGGTTCATAGCCAAACATGACAATTGGCCCATAGATGCGCCAAGGACCGCAAACACAAGACCCGTCCGCCAGGGCGCCCAAGGGCCGCGGGGTACACCggg                                         >  2:161212/2‑148 (MQ=255)                                                                                                         ccccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTg                                        >  2:377772/1‑150 (MQ=255)                                                                                                          cccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGc                                       >  1:1040218/1‑150 (MQ=255)                                                                                                           ccAGCCCGGCTGACACGCAATGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAAGCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGTTACACCGTTGcc                                      <  1:853244/150‑1 (MQ=255)                                                                                                              gCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGACAATGACCGCAATCAGTAGACCAATCAGCAAGTGAACCAAATGCCCGCGTGGTACCCCGTTGCCATc                                   >  2:209147/1‑150 (MQ=255)                                                                                                              gCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGAGCCAATGACCGCAATCAGTAGACCAAACAGCAAGTGAGCCAAATGGCCGCGTGGTACACCGTTGCCATc                                   >  2:169261/1‑150 (MQ=255)                                                                                                                   ccagccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCg                              <  1:914960/150‑1 (MQ=255)                                                                                                                   ccagccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCg                              <  2:1341360/150‑1 (MQ=255)                                                                                                                     agccagGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGtt                            <  1:968115/150‑1 (MQ=255)                                                                                                                        cagGCAAAGACTTTCTGACAGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGTGCCCATAGATGCGCCAATGACCGCATTCATTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAAc                         <  2:129686/150‑1 (MQ=255)                                                                                                                        cagGCAAAGACTTTCGGACCTAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAAc                         <  2:1195331/150‑1 (MQ=255)                                                                                                                         agGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACg                        <  1:1091666/150‑1 (MQ=255)                                                                                                                          ggCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGc                       >  2:739075/1‑150 (MQ=255)                                                                                                                           gCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGCAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGcc                      >  2:897872/1‑150 (MQ=255)                                                                                                                                  cTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCAAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCa               <  1:663809/150‑1 (MQ=255)                                                                                                                                  cTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAAGCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCa               >  1:174167/1‑150 (MQ=255)                                                                                                                                      cGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGccc           <  1:23285/150‑1 (MQ=255)                                                                                                                                      cGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGccc           >  2:1115822/1‑150 (MQ=255)                                                                                                                                        gACCGAAGTCGCGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCa         <  2:1285952/150‑1 (MQ=255)                                                                                                                                        gACCGAAGTCGCGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGAGCAGCCCCa         <  2:1280617/150‑1 (MQ=255)                                                                                                                                        gACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCC‑AAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCAt        >  1:407029/1‑150 (MQ=255)                                                                                                                                           cGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCa      <  1:1102160/150‑1 (MQ=255)                                                                                                                                               gTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAt  <  1:772689/150‑1 (MQ=255)                                                                                                                                               gTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAt  >  1:677542/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TCAGTTTGCGGTAGGCAAATGCACCTACAATCGCGCCAACGATAGGGCCGAAAAGCGGCACCAGGAAGTAAGGAATGTCTCTGCCGCCGGTAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCTGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGGGCCGCGTGGTACACCGTTGCCATCGTCCGTTAACGCCAGGATCAGCCCCATCAGAAT  >  NC_000913/4117328‑4117619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑