Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,257,226 C→G 7.3% R38G (CGT→GGT) ‡ lexA → transcriptional repressor of SOS regulon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,257,2260CG 7.3% 243.6 / 8.6 79R38G (CGT→GGT) ‡lexAtranscriptional repressor of SOS regulon
Reads supporting (aligned to +/- strand):  ref base C (37/36);  new base G (4/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TACTCACAGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAACC  >  NC_000913/4257077‑4257374
                                                                                                                                                     |                                                                                                                                                     
taCTCACAGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttcc                                                                                                                                                       <  1:606999/150‑1 (MQ=255)
taCTCACAGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGAATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttcc                                                                                                                                                       >  1:111761/1‑150 (MQ=255)
    cacaGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttccgttc                                                                                                                                                   >  1:553050/1‑150 (MQ=255)
    cacaGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGgttccgttc                                                                                                                                                   >  2:1118883/1‑150 (MQ=255)
      caGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTccc                                                                                                                                                 <  2:1167856/150‑1 (MQ=255)
        gCATAACTGTATATACACCCAGGGGGCTGAATGAAAGCGTTAACGGCCAGGCAACAAGATGTGTTTGATCTCATCCGTGATAACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCa                                                                                                                                               <  2:1305395/150‑1 (MQ=255)
        gCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCa                                                                                                                                               <  2:1310501/150‑1 (MQ=255)
           tAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAc                                                                                                                                            >  1:841414/1‑150 (MQ=255)
           aaaCTGTATATACACCCAGGGGGCGTAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGAACGGAACCCCAAAc                                                                                                                                            <  1:387224/149‑1 (MQ=255)
             aCTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGATATCGCGCAGCGTTTGGGGTTCCGTTCCCCAACcgc                                                                                                                                          >  1:225466/1‑150 (MQ=255)
             aCTGTATAAACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAAcgc                                                                                                                                          >  2:493705/1‑150 (MQ=255)
               tGTATAAACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGAACGGAACCCCAAAcgctg                                                                                                                                        <  1:866449/150‑3 (MQ=255)
                gTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGc                                                                                                                                       <  1:624053/150‑1 (MQ=255)
                   aataCACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTga                                                                                                                                    <  1:929090/149‑1 (MQ=255)
                      acacCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTgaaga                                                                                                                                 >  1:708011/1‑150 (MQ=255)
                                  cGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCACACGCGGCTGAAGAACATCTGAAGgc                                                                                                                     >  2:895535/1‑150 (MQ=255)
                                     aaTGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCt                                                                                                                  <  2:722763/150‑1 (MQ=255)
                                       tGAAAGCTTTAACGGCCAGGCTACAAGAGGGGGTTGATCTCATCCGTTATCACAGCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGGTCCGTTCCCCAAACGCGGCTGAAGATCATCTGAAGGCGCTgg                                                                                                                <  1:708265/150‑1 (MQ=255)
                                           aGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACg                                                                                                            <  2:391133/150‑1 (MQ=255)
                                           aGCGTCAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACg                                                                                                            >  1:692533/1‑150 (MQ=255)
                                            gCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGc                                                                                                           >  1:1095567/1‑150 (MQ=255)
                                              gTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCCAACGCGGCTGAAGACCATCTGAAGGCGCTGGCACGCaa                                                                                                         >  2:967819/1‑150 (MQ=255)
                                                   cGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCg                                                                                                    <  1:9818/150‑1 (MQ=255)
                                                     gCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCACACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGtt                                                                                                  >  1:180817/1‑150 (MQ=255)
                                                     gCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACTCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGtt                                                                                                  >  2:994152/1‑150 (MQ=255)
                                                       cAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTAt                                                                                                >  1:86226/1‑150 (MQ=255)
                                                         ggCAACAAGAGGTGTTTGATCTCATCCGTTATCACATCAGCCAGACAGGAATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCACAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTg                                                                                              <  1:831142/150‑1 (MQ=255)
                                                         ggCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCAGAAGGCGCTGGCCCGCAAAGGCGTTATTg                                                                                              >  1:1240248/1‑150 (MQ=255)
                                                          gCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGa                                                                                             >  1:67091/1‑150 (MQ=255)
                                                           caacaaGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGaa                                                                                            >  2:125631/1‑150 (MQ=255)
                                                           caacaaGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCACAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGaa                                                                                            >  2:1051332/1‑150 (MQ=255)
                                                                agagGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGt                                                                                      >  1:417203/1‑150 (MQ=255)
                                                                    gtgtTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTcc                                                                                  <  2:553050/150‑1 (MQ=255)
                                                                    gtgtTTGATCTCATCCGTGATCAAATCAGCCAGACATGTTAGCCGCCGACGCGTGCTGAAATCGCGCATCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTcc                                                                                  <  2:368162/150‑1 (MQ=255)
                                                                      gtTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCgg                                                                                >  1:1103061/1‑150 (MQ=255)
                                                                      gtTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCgg                                                                                >  2:1324156/1‑150 (MQ=255)
                                                                      gtTTGATCTCATCCGTGATCACATCAGCCAAACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCgg                                                                                <  2:892835/150‑1 (MQ=255)
                                                                       tttGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGgc                                                                               <  2:1266329/150‑1 (MQ=255)
                                                                       tttGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGgc                                                                               <  2:121532/150‑1 (MQ=255)
                                                                         tGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTTCCGgcg                                                                              >  2:65004/1‑150 (MQ=255)
                                                                         tGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGgcgc                                                                             >  2:1123278/1‑150 (MQ=255)
                                                                            tctcATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATc                                                                          <  1:1118883/150‑1 (MQ=255)
                                                                                aTCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCAcgc                                                                      >  2:487170/1‑150 (MQ=255)
                                                                                  ccGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCgg                                                                    <  1:497362/150‑1 (MQ=255)
                                                                                   cGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCggg                                                                   <  2:52087/150‑1 (MQ=255)
                                                                                         cacaTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGt                                                             >  1:752780/1‑150 (MQ=255)
                                                                                          acaTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTc                                                            <  2:1103061/150‑1 (MQ=255)
                                                                                          acaTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTc                                                            >  2:469802/1‑150 (MQ=255)
                                                                                           caTCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTTTTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCt                                                           >  1:928723/1‑150 (MQ=255)
                                                                                            aTCAGCGAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCAGGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTg                                                          <  1:255218/150‑1 (MQ=255)
                                                                                             tCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGt                                                         <  2:441706/150‑1 (MQ=255)
                                                                                             tCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGt                                                         <  2:718836/150‑1 (MQ=255)
                                                                                               aGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTg                                                       <  2:493862/150‑1 (MQ=255)
                                                                                               aGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTg                                                       >  2:885464/1‑150 (MQ=255)
                                                                                                   aGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCagg                                                   <  2:559010/150‑1 (MQ=255)
                                                                                                    gACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCagga                                                  >  2:966404/1‑150 (MQ=255)
                                                                                                    gACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCagga                                                  >  2:153370/1‑150 (MQ=255)
                                                                                                      cAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCaggaag                                                >  1:554372/1‑150 (MQ=255)
                                                                                                          tATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCaggaagagga                                            <  1:1254871/150‑1 (MQ=255)
                                                                                                            tGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACTCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCaggaagaggaag                                          <  1:994152/150‑1 (MQ=255)
                                                                                                               cgccgACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACCTCTGAAGGCGCTGGCACGCAAAGGGGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGaagaag                                       >  2:1234257/1‑150 (MQ=255)
                                                                                                                     cgcgTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGc                                 <  1:446120/150‑1 (MQ=255)
                                                                                                                      gcgTGCGGAAATCGCGCGGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGcc                                >  1:1253791/1‑150 (MQ=255)
                                                                                                                       cgTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCg                               >  2:61000/1‑150 (MQ=255)
                                                                                                                       cgTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCg                               >  1:564307/1‑150 (MQ=255)
                                                                                                                           cGGAAATCGCTCAGCGTTTGGGTTTCCGTTCACCAAACGCGGCTGAAGAACATCTGAAGGCGCTTGCACGCAAAGGCGTTATTGAAATTGTTTCCTGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGATGAAGAAGGGTTGCAGCTgg                           <  2:1010934/150‑1 (MQ=255)
                                                                                                                             gAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCGGAAGAACAGCTGAAGGCGCTGGCGCGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATGCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTa                         >  1:175608/1‑150 (MQ=255)
                                                                                                                                aTCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGt                      <  1:125173/150‑1 (MQ=255)
                                                                                                                                aTCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGt                      <  1:889409/150‑1 (MQ=255)
                                                                                                                                  cgcgcgGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCg                    <  2:1253791/150‑1 (MQ=255)
                                                                                                                                   gcgcAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCGGAAGAACAGCTGAAGGTGCTGGCACGCAAAGGCGGTATTGAAATTGTTTCCGGCGCATCACGCGGGAGTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGGAGGTCgt                   >  1:121660/1‑150 (MQ=255)
                                                                                                                                    agccgCGTTTGGGGAACGGAACCCCAAACGCTGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCgtg                  >  1:339680/5‑150 (MQ=255)
                                                                                                                                     gccgCGTTTGGGGAACGGAACCCCAAACGCTGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCgtgt                 >  2:22645/4‑150 (MQ=255)
                                                                                                                                     gccgCGTTTGGGGAACGGAACCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCgtgt                 >  2:404515/4‑150 (MQ=255)
                                                                                                                                       aGCGGTTGGGGGTCCGTTCCCCAATCGGGGCGGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTCTTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTgg               <  1:126021/150‑1 (MQ=255)
                                                                                                                                        gCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGc              <  2:374766/150‑1 (MQ=255)
                                                                                                                                         cGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCt             <  2:708011/150‑1 (MQ=255)
                                                                                                                                         cGTTTGGGGAACGGAACCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCt             >  1:1343367/1‑150 (MQ=255)
                                                                                                                                           tttGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGc           <  2:180817/150‑1 (MQ=255)
                                                                                                                                            ttGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGcc          <  2:1341728/150‑1 (MQ=255)
                                                                                                                                             tGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAATTTGTTTCCGGCGCATCACGCGGGCTTCGTCTGTTGCAGGAAGAGGAAGAAGTGTTGCCGCTGGTAGGTCGTGTGGCTGCCg         >  2:643386/1‑150 (MQ=255)
                                                                                                                                             tGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCg         >  1:1282313/1‑150 (MQ=255)
                                                                                                                                               gggTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGCTTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGt       >  1:375534/1‑150 (MQ=255)
                                                                                                                                                 gttccgttacCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGACGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGa     >  1:190543/1‑150 (MQ=255)
                                                                                                                                                    ccgttccCCATACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTTTTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAAcc  <  2:1264735/150‑1 (MQ=255)
                                                                                                                                                    ccgttccCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAAcc  <  2:67091/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACTCACAGCATAACTGTATATACACCCAGGGGGCGGAATGAAAGCGTTAACGGCCAGGCAACAAGAGGTGTTTGATCTCATCCGTGATCACATCAGCCAGACAGGTATGCCGCCGACGCGTGCGGAAATCGCGCAGCGTTTGGGGTTCCGTTCCCCAAACGCGGCTGAAGAACATCTGAAGGCGCTGGCACGCAAAGGCGTTATTGAAATTGTTTCCGGCGCATCACGCGGGATTCGTCTGTTGCAGGAAGAGGAAGAAGGGTTGCCGCTGGTAGGTCGTGTGGCTGCCGGTGAACC  >  NC_000913/4257077‑4257374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: