Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,004,963 T→A 7.1% E275V (GAG→GTG)  ygeV ← putative sigma‑54‑interacting transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,004,9630TA 7.1% 1039.8 / 6.7 342E275V (GAG→GTG) ygeVputative sigma‑54‑interacting transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (114/188);  new base A (32/2);  total (152/190)
Fisher's exact test for biased strand distribution p-value = 6.91e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 346 total aligned reads displayed.

TAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGAA  >  NC_000913/3004814‑3005112
                                                                                                                                                      |                                                                                                                                                      
  aaGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTTATTTGCGGATTACACGGGACtgg                                                                                                                                                      >  1:1986828/1‑148 (MQ=255)
      gTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGccaa                                                                                                                                                 >  2:5573087/1‑150 (MQ=255)
        ttATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGccaacc                                                                                                                                               <  2:6722713/150‑1 (MQ=255)
            tCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAtt                                                                                                                                           <  1:6192764/150‑1 (MQ=255)
            tCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAtt                                                                                                                                           <  2:5951785/150‑1 (MQ=255)
               gTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCCACCAATTGtt                                                                                                                                        >  1:3486949/1‑150 (MQ=255)
               gTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGtt                                                                                                                                        <  2:4889393/150‑1 (MQ=255)
               gTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACACCCCCACCAAATGtt                                                                                                                                        >  2:1970277/1‑150 (MQ=255)
                 cTTCCGCACAACATATGGATTGCACGGGCGACGACTACATTACCCCTGCCGCTTACACCAACCACCAAAACCCTGCATGGGCTGCGTGCAATACGGCTAATGAGTCGATTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCa                                                                                                                                      <  2:3646328/150‑1 (MQ=255)
                  ttCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAGCACGGCACTCGCCGACCAATTGTTCaa                                                                                                                                     <  1:1102531/150‑1 (MQ=255)
                  ttCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCaa                                                                                                                                     <  2:3151051/150‑1 (MQ=255)
                  ttCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCaa                                                                                                                                     <  2:5789909/150‑1 (MQ=255)
                     cGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCAAAACACGGCACTCGCCAACCAAATGTTCAatat                                                                                                                                  >  2:2223552/1‑150 (MQ=255)
                        tCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCg                                                                                                                               <  1:3073944/150‑1 (MQ=255)
                           aCTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGtt                                                                                                                            <  2:6655279/150‑1 (MQ=255)
                             ttATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGccaac                                                                                                                                                <  1:6678351/128‑1 (MQ=255)
                               aTGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTtcatc                                                                                                                        <  1:1803928/150‑1 (MQ=255)
                                tGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTtcatca                                                                                                                       <  2:2445648/150‑1 (MQ=255)
                                 ggATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAg                                                                                                                      <  1:3245235/150‑1 (MQ=255)
                                        aCGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCAAAAAACGGCACACGCCAACCAAATTTTCAAAATGCGGGTAATCAGGTGCAtt                                                                                                               >  1:6753926/1‑150 (MQ=255)
                                            gCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCt                                                                                                           <  1:5279250/150‑1 (MQ=255)
                                             cgacgaCTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTa                                                                                                          <  2:4102855/150‑1 (MQ=255)
                                             cgacgaCTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGACGCATAACAACACACACGCCAAACAAAAGTTCAATATTCGGTTAATCAGGTGCATTTTCTa                                                                                                          >  1:6365173/1‑150 (MQ=255)
                                              gacgacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCATAAAAAGGCACACGCCAACCAAATGTTAAAAATTCGGTTCATCAGGTGCCTTTTCTAc                                                                                                         >  2:2903908/1‑150 (MQ=255)
                                              gacgacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCCACCAATTGTTCAATATGCGGTTCATCAGGTGCAGTTGCTAc                                                                                                         >  2:4416781/1‑150 (MQ=255)
                                               acgacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACa                                                                                                        <  2:6257702/150‑1 (MQ=255)
                                               acgacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACa                                                                                                        <  1:116441/150‑1 (MQ=255)
                                                cgacTTCTTTACCCGTGCCGCTTTCACCACCCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAg                                                                                                       <  2:6477956/150‑1 (MQ=255)
                                                cgacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAg                                                                                                       <  2:2815322/150‑1 (MQ=255)
                                                 gacTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCATAACAACGCACTCGCCAACCAAATGTTAAATATGCGGTTCATAAGGTGCAATTGCTACAGa                                                                                                      >  2:1286944/1‑150 (MQ=255)
                                                     tcttTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTTATTGCCGCATAAAACGGCACTCGCCAACCAAATGTTAAATATGCGGTTCATCAGGTTCATTTTCTACAGagaaa                                                                                                  >  2:743683/1‑150 (MQ=255)
                                                     tcttTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGaaaaa                                                                                                  <  2:5141447/150‑1 (MQ=255)
                                                       tttACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCAAAAAACCGCACACGCCCAACCAAATTTAAAAATTAGGGTAATCAGGTGCAGGTTATACAGaaaaaaa                                                                                                >  1:4005678/1‑148 (MQ=255)
                                                       tttACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACt                                                                                                <  2:4829396/150‑1 (MQ=255)
                                                        ttACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTg                                                                                               <  1:3794374/150‑1 (MQ=255)
                                                         tACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTgg                                                                                              >  2:3895193/1‑150 (MQ=255)
                                                           cccGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTTAATTGCGCAAAAAACCGCCAAAGCCAACCAAATGTTAAAAATTCGGTTGATAAAGTGGATTTTATACAaaaaaaaattta                                                                                            >  1:4620226/1‑144 (MQ=255)
                                                             cGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATg                                                                                          <  2:3020994/150‑1 (MQ=255)
                                                                  cGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGAtt                                                                                     <  2:32965/150‑1 (MQ=255)
                                                                  cGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTGTTTATTGCCGCCAAAAACCGCACGCGCCAACCAAAAGTTAAATATTCGGTTAATCAGGTGCAGTTTGTACAGAAAAAAAGGTATTCGttt                                                                                     >  1:1750610/1‑150 (MQ=255)
                                                                  cGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTGTTAATTGCCGCCTAACACGGCACGCGCCAACCCAATGTTCAATATGCGGTTCCACAGGTGCCTTTGCTACAGAAAAACAGGGATGCgctg                                                                                     >  2:5814877/1‑147 (MQ=255)
                                                                     tttCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGt                                                                                  <  2:2192504/150‑1 (MQ=255)
                                                                       tCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATATCACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGa                                                                                <  1:593268/150‑1 (MQ=255)
                                                                       tCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACACGCCCACCAAATGTTCAATATTCGGTTAATAAGGTGCAATTGCTAAAGAAAAAAAGGTATGCGATTGGTGa                                                                                >  1:5604112/1‑150 (MQ=255)
                                                                         accaaccaCCCTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGaaa                                                                              <  1:4276132/150‑1 (MQ=255)
                                                                         accaaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGaaa                                                                              <  2:6160624/150‑1 (MQ=255)
                                                                          ccaaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTGATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAAc                                                                             <  2:3590447/150‑1 (MQ=255)
                                                                           caaccaCCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACACGCCAACCAAATGTTCAATAAGCGGTTCATCAGGTGCAATTGCTACAGAAAAACTGGTATGCGATTGGTGaaaag                                                                            >  2:5459977/1‑148 (MQ=255)
                                                                             accaccATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGcc                                                                          <  1:2273402/150‑1 (MQ=255)
                                                                               caccaTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCAt                                                                        <  2:4521186/150‑1 (MQ=255)
                                                                               caccaTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCAt                                                                        <  2:2746503/150‑1 (MQ=255)
                                                                               caccaTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAATGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGccct                                                                        >  2:909911/1‑148 (MQ=255)
                                                                                 ccaTAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTa                                                                      <  2:4625862/150‑1 (MQ=255)
                                                                                    tAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaa                                                                   <  1:1026882/150‑1 (MQ=255)
                                                                                    tAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaa                                                                   <  2:3631676/150‑1 (MQ=255)
                                                                                     aaCGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTTCCGCAAAAAACCGCACTCCCCAACCAAATGTTCAATATGCGGTTAATAAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaaa                                                                  >  2:23643/1‑150 (MQ=255)
                                                                                     aaCGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTaaaaa                                                                  <  1:5786302/150‑1 (MQ=255)
                                                                                        gCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACAAGGCACTCCCCAACCAAATTTTCAATATTCGGGTAATAAGGTGCAATTTCTACAAAAAAACAGGTATTCGATTTGTGAAACACCATTaaaaaaaa                                                               >  1:3572850/1‑150 (MQ=255)
                                                                                            gATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAAAACGGCACTCGCCAAACAATTGTTCAATATGCGGTTCATCAGGTGCATTTTCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCAATAAAAATAAtttt                                                           >  2:1388811/1‑148 (MQ=255)
                                                                                                ggCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGc                                                       <  1:4063382/150‑1 (MQ=255)
                                                                                                ggCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAAATGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGc                                                       >  2:3488581/1‑150 (MQ=255)
                                                                                                 gCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGcc                                                      >  2:1547176/1‑150 (MQ=255)
                                                                                                 gCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGcc                                                      <  1:2759653/150‑1 (MQ=255)
                                                                                                   tGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCt                                                    <  2:1615999/150‑1 (MQ=255)
                                                                                                        gCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTTCTAAAGAAAAAATGGTATTCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAAtt                                               >  2:6541260/1‑149 (MQ=255)
                                                                                                         cAATACGGCTAATGAGTCGTTTTAATTTCCGCAAAAAACCGCACGCGCCAACCAAATGTTAAAAATGCGGTTAAAAAGGTGCAATTTCAAAAGAAAAAAAGGTATTCGATTGGTGTAAAGCCAATAAAAATAATTTTCGGCCCGGAAAGt                                              >  2:1316/1‑150 (MQ=255)
                                                                                                          aaTACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGtt                                             <  2:2262255/150‑1 (MQ=255)
                                                                                                            tACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTAt                                           <  1:2789251/150‑1 (MQ=255)
                                                                                                             aCGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATg                                          <  1:974124/150‑1 (MQ=255)
                                                                                                                gCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCaa                                       <  2:1475433/150‑1 (MQ=255)
                                                                                                                    aTGAGTCGTTTTAATTTCCGCATAACACGGCACACCCCAACCAAATGTTCAATATGCGGTTCATCAGGTGCAATTTCTACAGAAAAACTGGTATGCGATTTGTGAAACCCCATTAAAAATAAATGTCGGCCCAGAATGTTATGCAAttta                                   >  2:1808994/1‑148 (MQ=255)
                                                                                                                     tGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGAc                                  <  2:2266610/150‑1 (MQ=255)
                                                                                                                     tGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACAGGTATGCGATTGGTGAAACGCCATTAAAAAAAAATGTCGGCCCAGAATGTTATGCAAATGAc                                  >  2:6124088/1‑150 (MQ=255)
                                                                                                                       aGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCa                                <  2:3825376/150‑1 (MQ=255)
                                                                                                                        gTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCaa                               >  2:5192021/1‑150 (MQ=255)
                                                                                                                           gTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGc                                                                                                                                >  1:1525594/1‑50 (MQ=255)
                                                                                                                              tttAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGAtt                          <  2:2014376/150‑1 (MQ=255)
                                                                                                                               ttAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTa                         >  1:2417896/1‑150 (MQ=255)
                                                                                                                                tAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTaa                        <  2:908518/150‑1 (MQ=255)
                                                                                                                                tAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTaa                        <  1:6596215/150‑1 (MQ=255)
                                                                                                                                  aTTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAAtt                      >  2:6609433/1‑150 (MQ=255)
                                                                                                                                    tGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCa                    <  2:4966558/150‑1 (MQ=255)
                                                                                                                                    tGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCa                    >  1:2229269/1‑150 (MQ=255)
                                                                                                                                    tGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCa                    <  1:3008273/150‑1 (MQ=255)
                                                                                                                                       cGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACtt                 >  2:4701491/1‑150 (MQ=255)
                                                                                                                                       cGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACtt                 >  1:3340084/1‑150 (MQ=255)
                                                                                                                                       cGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACtt                 <  2:638922/150‑1 (MQ=255)
                                                                                                                                          aTAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTa              <  2:4228737/150‑1 (MQ=255)
                                                                                                                                           tAACACGGCACACGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTAt             <  1:4090983/150‑1 (MQ=255)
                                                                                                                                            aaCACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc            <  1:4923606/150‑1 (MQ=255)
                                                                                                                                            aaCACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc            <  1:747393/150‑1 (MQ=255)
                                                                                                                                            aaCACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc            <  1:5405940/150‑1 (MQ=255)
                                                                                                                                            aaCACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc            <  1:497466/150‑1 (MQ=255)
                                                                                                                                               acGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTc         <  1:259840/150‑1 (MQ=255)
                                                                                                                                                 ggCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTccc       <  1:895553/150‑1 (MQ=255)
                                                                                                                                                   cACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCAt     >  1:6114914/1‑150 (MQ=255)
                                                                                                                                                    aCTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGt                                                                                  <  1:1382763/72‑1 (MQ=255)
                                                                                                                                                    aCTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCACTTGACCAATGATTCATTCACTTTTATCGTCCCATg    >  2:3534327/1‑150 (MQ=255)
                                                                                                                                                     cTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGa   >  1:4342705/1‑150 (MQ=255)
                                                                                                                                                     cTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAAGTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGa   >  2:1147672/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGAA  >  NC_000913/3004814‑3005112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: