Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,523 G→T 5.9% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5230GT 5.9% 720.1 / 2.3 240L72I (CTT→ATT) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (129/87);  new base T (4/19);  total (133/107)
Fisher's exact test for biased strand distribution p-value = 1.10e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238379‑238672
                                                                                                                                                |                                                                                                                                                     
tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                                  <  1:1753910/150‑1 (MQ=255)
 gACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAt                                                                                                                                                 <  1:4676524/150‑1 (MQ=255)
 gACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAt                                                                                                                                                 <  2:5649943/150‑1 (MQ=255)
  aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaag                                                                                                                                                       >  2:4268666/1‑143 (MQ=255)
    atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              <  2:1935549/150‑1 (MQ=255)
     tatCCTGTGACAACAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                             <  2:4319521/150‑1 (MQ=255)
      aaccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGATAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAATTAAAATTACAt                                                                                                                                            >  1:4051419/3‑150 (MQ=255)
          tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                        >  1:5458976/1‑150 (MQ=255)
                   aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:1332362/150‑1 (MQ=255)
                   aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                                               <  1:5488978/150‑1 (MQ=255)
                    ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  2:6021709/1‑150 (MQ=255)
                    ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                              >  2:4970826/1‑150 (MQ=255)
                      aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                            >  2:1648840/1‑150 (MQ=255)
                      aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                            <  2:105169/150‑1 (MQ=255)
                       gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                           <  1:1220095/150‑1 (MQ=255)
                          aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGGTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGaaaaa                                                                                                                        >  1:2954018/1‑149 (MQ=255)
                          aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        >  2:1091956/1‑150 (MQ=255)
                          aTTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAc                                                                                                                        <  1:4111935/150‑1 (MQ=255)
                             aaaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGAc                                                                                                                     <  2:1406004/150‑1 (MQ=255)
                               aGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGatat                                                                                                                   >  1:3855133/1‑150 (MQ=255)
                                ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                  >  2:803207/1‑150 (MQ=255)
                                ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                  >  1:1959479/1‑150 (MQ=255)
                                ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATAGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAATGAAAACTAAATg                                                                                                                  >  1:4754447/1‑150 (MQ=255)
                                 gTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGa                                                                                                                 >  2:5986851/1‑150 (MQ=255)
                                   gCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                                               <  2:3084214/150‑1 (MQ=255)
                                      aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                                            >  2:1795999/1‑150 (MQ=255)
                                       ttACGATAGCTATTAGTAAAAATATAAGTGTTAGCTGTATTGTTAGGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATGGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                                           <  2:750760/150‑1 (MQ=255)
                                       ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                                           >  1:5655116/1‑150 (MQ=255)
                                          cGATAGATATTAGTAAAAATATAAGAGTTAGCTGTTTTGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGc                                                                                                        <  2:617876/150‑1 (MQ=255)
                                            aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                      >  1:5632815/1‑150 (MQ=255)
                                                cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                  <  2:1105736/150‑1 (MQ=255)
                                                 tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaaa                                                                                                 >  2:5150034/1‑149 (MQ=255)
                                                 tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAAt                                                                                                 >  1:6278014/1‑150 (MQ=255)
                                                  tttattaAAATTATAATTTTTTGCTTTTTTTTTTTGTCTGTGGATAATTTGACTACATTCTTTTTTTTTTTTATTATTTTTTTTTTTATCTTAATTAAATTTAATTTAATTTTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATc                                                                                                <  1:5062722/149‑1 (MQ=255)
                                                  aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAATTAAAAAGACATGAAACCGAAGCACAGAATc                                                                                                >  1:227043/1‑150 (MQ=255)
                                                     tttAAAAATATAATTTTTAGTTGTTTTGTTTTTTCTGTGGCTATATTTACTACCTTCTTTTTTTTTATTAATAATTTTTTTTTTATCGTATTAAAAATTAAATTAATTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                             <  1:4934834/148‑1 (MQ=255)
                                                     cgTAAAAATATAAGTTTTCGGTTTTTTTTTTTGTGTGTGGCTAATTTGACTCCATTGTTTTTTTTGTTTAATATTTTTTTTTTTATCTTAATTAAAATTACATGATTATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                                             <  1:1869704/149‑1 (MQ=255)
                                                       tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGCAAAGAATCAACaa                                                                                           >  1:2770119/1‑149 (MQ=255)
                                                       tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACAt                                                                                           >  2:6239182/1‑150 (MQ=255)
                                                        aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                          >  2:1199525/1‑150 (MQ=255)
                                                         aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGGAAGTAAAAAAAAATTAATATTTAAAAATGCAAAAGACATGAAAACCAAGCACAGAAACAACAtta                                                                                         >  1:2551440/1‑149 (MQ=255)
                                                         aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACAAGAAAACGAAGCACAGAATAAACAtta                                                                                         >  1:6107567/1‑149 (MQ=255)
                                                         aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATTAATATTTAAAAAGGAAAAAGACAATAAACAGAAGCACAGAATCAACATtc                                                                                         >  2:2312051/1‑150 (MQ=255)
                                                               taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAt                                                                                   >  2:4149752/1‑150 (MQ=255)
                                                                aaGTGTTTGCTTTTTTGTTTTTTTTGTGGCGAAATTGACTACCTTCTTTTTTTTTATTAATATTTATTTTATTTTCTTAAGTAAAATTACATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                                  <  1:6078983/150‑1 (MQ=255)
                                                                aaGAGTTAGCTTTTTTTTTATGTCTGTGGCTAAATTTACTACCTTATTTTTTTTTATTAATATTTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATc                                                                                  <  1:3590715/150‑1 (MQ=255)
                                                                   agTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACATa                                                                               >  2:2577842/1‑150 (MQ=255)
                                                                     ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                             >  2:4682987/1‑150 (MQ=255)
                                                                     ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATaaa                                                                             >  1:1024946/1‑150 (MQ=255)
                                                                          tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAataat                                                                        >  2:3397677/1‑148 (MQ=255)
                                                                          tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATGAATATTTAAAAATGAAAACGACAAGAAACCGAAGCACAGAAACAACATTCTCCAAAAAAAAAAAAtt                                                                        >  1:1047923/1‑150 (MQ=255)
                                                                           ttATTTTTTTTTCTGTGGCTAATTTGAATACCTTCTTTTTTTTTATTAATATTTTTTTTATTATCTTAATTAAAATTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       <  1:5760068/149‑1 (MQ=255)
                                                                           gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAttt                                                                       <  1:3556132/150‑1 (MQ=255)
                                                                            tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATAGTTAGTAAAATTACATTAATATTTAAAAATGAAAACGACATGAAAACGAAGCACAGAATAAAAATTATCCAAAAATAAAATAttta                                                                      >  1:708737/1‑149 (MQ=255)
                                                                               tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                   <  2:3449977/150‑1 (MQ=255)
                                                                                gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                  >  2:1301680/1‑150 (MQ=255)
                                                                                 ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              >  2:1536743/1‑73 (MQ=255)
                                                                                 ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                              <  1:1536743/73‑1 (MQ=255)
                                                                                   aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGTTTTTATTATAGTAAGTAAAATTAAAAGAATATTTAAAAAGGAAAAAGACATGAAAACGAAGCACAGAATCAACAATCTCCAATCATAAAATATTTACGTGGAg                                                               >  1:3941578/1‑150 (MQ=255)
                                                                                     gTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTACGTGGAGCa                                                             >  2:6291249/1‑150 (MQ=255)
                                                                                        tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGAAAACGAAGCACAGAATAAAAAATATCCAAAAATAAAAAAATTAACTGGGGCAttt                                                          >  1:1460916/1‑150 (MQ=255)
                                                                                        tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                                          >  2:5721350/1‑150 (MQ=255)
                                                                                          tgGCTAAATTTACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        <  2:2770119/150‑1 (MQ=255)
                                                                                          tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                        >  2:173549/1‑150 (MQ=255)
                                                                                              gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                                    <  2:5903376/150‑1 (MQ=255)
                                                                                               aaaTTGACTACCTTCTTTTTTTTGATTAATAATGATTTTATTATCGTATGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  2:4567377/150‑1 (MQ=255)
                                                                                               aaaTTGAATACCTTCTTTTTTTTTATTAATAATGATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                   <  1:1730106/150‑1 (MQ=255)
                                                                                                 aTTGACTACCATCGTTTTTTTTATTAAGAATGATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                                 <  1:3695884/150‑1 (MQ=255)
                                                                                                  tttaCTAACTTCTTTTATTTGATTAATCATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                <  2:4051419/147‑1 (MQ=255)
                                                                                                       tACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATag                                           >  2:4426835/1‑150 (MQ=255)
                                                                                                         ccTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  2:3745958/150‑1 (MQ=255)
                                                                                                         ccTTCGTTTTTTTGATTAATAATTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  1:5849681/150‑1 (MQ=255)
                                                                                                         ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                         <  2:227043/150‑1 (MQ=255)
                                                                                                           ttCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATACATGAATATTTAAAAAGGAAAACCACAAGAAACCGAAGCACAGAAACAACAATCTCCCAACAAAAAATAATTCCCTGGAGCATTTTATTATTGAAAATAGAGGt                                       >  1:6652984/1‑150 (MQ=255)
                                                                                                            tCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGtt                                      >  2:1918283/1‑150 (MQ=255)
                                                                                                                ttttttTATTAAGAATGATTTTATTATCTTAAGTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  2:2681041/150‑1 (MQ=255)
                                                                                                                ttttttGATTAAGAATGATTTTTTTTTCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAAc                                  <  2:2058872/150‑1 (MQ=255)
                                                                                                                 tttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACt                                 >  2:6706471/1‑150 (MQ=255)
                                                                                                                  ttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTc                                >  2:5468255/1‑150 (MQ=255)
                                                                                                                   tttGATTAAGAATGATTTTATTATCGTAAGTAATATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               <  2:1592739/150‑1 (MQ=255)
                                                                                                                   tttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                               >  1:6153054/1‑150 (MQ=255)
                                                                                                                    tttaTTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                              <  2:4118376/147‑1 (MQ=255)
                                                                                                                       aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                           >  1:2878875/1‑150 (MQ=255)
                                                                                                                        ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa                          <  2:5732612/150‑1 (MQ=255)
                                                                                                                           aGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaa                       >  2:5420589/1‑150 (MQ=255)
                                                                                                                             aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                     >  2:6429930/1‑150 (MQ=255)
                                                                                                                             aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                     >  1:228956/1‑150 (MQ=255)
                                                                                                                               tGATTTTATTATCTTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa                   <  2:5215130/150‑1 (MQ=255)
                                                                                                                                gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                  <  2:4802185/150‑1 (MQ=255)
                                                                                                                                 aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                 >  2:6491779/1‑150 (MQ=255)
                                                                                                                                  ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                >  1:6545013/1‑150 (MQ=255)
                                                                                                                                  ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                >  1:6755663/1‑150 (MQ=255)
                                                                                                                                  ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                >  1:4388335/1‑150 (MQ=255)
                                                                                                                                  ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                >  1:903569/1‑150 (MQ=255)
                                                                                                                                       ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           >  1:1112819/1‑150 (MQ=255)
                                                                                                                                       ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAt           >  1:5266411/1‑150 (MQ=255)
                                                                                                                                        tatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCAtt          >  2:880553/1‑150 (MQ=255)
                                                                                                                                          tCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGa        <  1:4923126/150‑1 (MQ=255)
                                                                                                                                               agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa   >  2:1259803/1‑150 (MQ=255)
                                                                                                                                                gtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAg  <  2:3176176/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAG  >  NC_000913/238379‑238672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: