Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,630 A→T 11.9% intergenic (+85/‑314) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6300AT11.9% 669.5 / 19.7 257intergenic (+85/‑314)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base A (167/44);  new base T (2/34);  total (170/87)
Fisher's exact test for biased strand distribution p-value = 8.25e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

TGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGT  >  NC_000913/3239481‑3239776
                                                                                                                                                     |                                                                                                                                                  
     atgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                                                                                                               >  2:5186344/1‑150 (MQ=255)
       gaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACGCTGTCATTTTCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAAt                                                                                                                                             >  2:2544224/1‑150 (MQ=255)
          gcttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                          >  2:940248/3‑150 (MQ=255)
          gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                          >  2:1138438/1‑150 (MQ=255)
          gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                                          >  2:2855393/1‑150 (MQ=255)
           tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                                         >  1:3757692/1‑150 (MQ=255)
            tttttatCAACGCCTGGGTGAATTATCGGCTTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTCGGGGTTAGGGTAATTTTTc                                                                                                                                        <  2:2252946/147‑1 (MQ=255)
             tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                                       >  1:3080311/1‑150 (MQ=255)
              attatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGc                                                                                                                                      >  1:1622370/1‑150 (MQ=255)
                tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                                    >  2:2477987/1‑150 (MQ=255)
                       gCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGcg                                                                                                                             >  1:3237479/1‑149 (MQ=255)
                       gCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCt                                                                                                                             >  2:1595945/1‑150 (MQ=255)
                        cgtGGGTGATTTTTGGGATTTATAAGCGGGGGGGTGTATTTTTTTTATTCTGCCTATGCGGTTTACCCTTTAATTAACTTGCGCACGTTTTTCCCCCTCGCCCTCCCCGGGTGTGGGTGGGGGTGTGGTTAATTTTTCGCACCGATGCTg                                                                                                                            <  2:273707/148‑1 (MQ=255)
                          ggggTTAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTACGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGTTAATTTTTCGCACCGATGCTGGc                                                                                                                          <  2:6678514/149‑1 (MQ=255)
                            gtttatttATCGGCATTATAAGCAGGGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGACTTTAACATGAGCACCGTTTTCTCCCCCTCCCTTCCCGGGTGCGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCt                                                                                                                        <  2:3161139/144‑1 (MQ=255)
                                  ttATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                                                                                                                  >  2:6600834/1‑150 (MQ=255)
                                   tATCGGCTTGATAAGCTGGGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                                                 <  2:4992107/150‑1 (MQ=255)
                                    aTCGGCATTATAAGCAGGGGGGTGGATATTTTTTAATCTGCATAAGCCGTGTACCCGGTCTTTAACATGAGCACCGTTTTCTCCCCCTCCCTCCCCGGGAGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                                                <  2:5604306/150‑1 (MQ=255)
                                       ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                                             <  2:5039202/150‑1 (MQ=255)
                                       ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTGTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                                             >  2:4928188/1‑150 (MQ=255)
                                        gCATGTTATGCTGGGGGGTGTATATTTTTTAATGTGCCTACGCGGTGTACCCTGTCTTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGTGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                                            <  2:3702121/150‑1 (MQ=255)
                                        gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                                            >  2:1747802/1‑150 (MQ=255)
                                        gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                                            >  1:2601991/1‑150 (MQ=255)
                                        gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                                            >  1:1187457/1‑150 (MQ=255)
                                           tGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTa                                                                                                         >  1:3338527/1‑150 (MQ=255)
                                            taTAAGCCGGGGGGTTTATAATTTTTAATCTGCAAAAGCCGTGTACCCTCTCATTAACTTGAGCACCTTTTTCACCCTCCCCCTCCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                                        <  2:6358597/149‑1 (MQ=25)
                                               aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCGTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                                     >  2:1032782/1‑150 (MQ=255)
                                                  cagcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                                                  >  1:6389988/1‑150 (MQ=255)
                                                           ggATAATTTTTAATCGGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCGCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACg                                                                                         <  2:4388250/150‑1 (MQ=255)
                                                             aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACAGATGCTGGCGTGTTCCCCTCACCCTAACCCTGTCCCCAAACggg                                                                                       >  2:5856620/1‑150 (MQ=255)
                                                                 tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                                   >  2:1422351/1‑150 (MQ=255)
                                                                 tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCTCCCCAAACGGGGCGa                                                                                   >  2:775053/1‑150 (MQ=255)
                                                                  ttttAATCTCCCTCAGCCTGGTACCCTTTCATTAACCTGCGCCCGTTTTTCGCCCCCGCCCTGCCGGGGTGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                                  <  1:1007463/150‑1 (MQ=18)
                                                                   tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCACACGGGGCGAgg                                                                                 >  2:6460543/1‑150 (MQ=255)
                                                                   tttAATCTGCATACGCGGTTTACCCTTTAATTAACATGAGCACGGTTTTCTCCCTCTCCCTTCCTGGGAGTGGTTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                                                 <  2:1134689/150‑1 (MQ=25)
                                                                         cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGTCGAGGGGCGGGGTGGGGTTATTTTTACCAACCGAGGCGGCCCGGTTCCCCGCACCCTACCCCACACCCCACACGGGGCggggggccgg                                                                           >  2:3580479/1‑146 (MQ=255)
                                                                          tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                                          >  2:3439700/1‑150 (MQ=255)
                                                                            ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCCCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                                        >  1:5351315/1‑150 (MQ=255)
                                                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                                       >  1:2329984/1‑150 (MQ=255)
                                                                             cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCACACCCCAAACCGGGCGAGGGGACTGACCg                                                                       >  1:5220299/1‑150 (MQ=255)
                                                                              tccgcggtggACCCTGTCATTACCATGCGCCCGGTTTGCGCCCCCCCCCGTCCGGGGTGGGGGGGGGGGTGCGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                      <  1:3032879/144‑1 (MQ=18)
                                                                              tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                                      >  2:4525306/1‑150 (MQ=255)
                                                                                     tgtACCCTGTCATTAACTGGCGCCCCGTTTCCCCCCTCCCCCTGCCTGGTTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttt                                                               <  2:809952/150‑1 (MQ=21)
                                                                                     tgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCACACGGGGCGAGGGGACTGACCGAGTCCttt                                                               >  1:461309/1‑150 (MQ=255)
                                                                                       tACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACACCCCAAACGGGGCGCGGGGACTGACCGAGtagatcgg                                                             >  1:2957245/1‑143 (MQ=255)
                                                                                        ccccggccATTAACCGGCGCACCGTTTTCGCCCGCCCCCTGCGGGGGAGTGGGGGGGGGTGTGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                            <  2:4734489/143‑1 (MQ=21)
                                                                                        ccccTGACTTTAACCTCCGCACGGTTTCCCCCCCCCCCCGCCCGGGGCGTGGGGGGGGGTGTGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                            <  1:5221661/149‑1 (MQ=16)
                                                                                        aCCCTGTCATTAACATGAGCACGGTGTGCGCCCTCGCCCTGCCCGGGAGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                            <  2:6104100/150‑1 (MQ=25)
                                                                                        aCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                                            >  2:3488237/1‑150 (MQ=255)
                                                                                          ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                                          >  2:5108034/1‑150 (MQ=255)
                                                                                          ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                                          >  2:6512088/1‑150 (MQ=255)
                                                                                           cTGTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGTGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                                         <  1:6198422/150‑1 (MQ=37)
                                                                                             gTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCATTTTTgtttt                                                       >  2:6272866/1‑146 (MQ=255)
                                                                                              tCATTACCTTGTGCCCGGTTTTCTCCCTCACGATTCCCGGGTGTGGGTGGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgtt                                                      <  1:1061539/150‑1 (MQ=21)
                                                                                                aTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                                    <  2:4250995/150‑1 (MQ=255)
                                                                                                 ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAGGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGCTGTTGtc                                                   >  2:1321181/1‑150 (MQ=255)
                                                                                                 ttAACATGAGCACCGTTTTCTCCCTCTACCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTAGCACCGATGCTGGCATGTTCCCCTAACCCAAACCCTCTCCCCAAACGGGGCGACGGGCCTGACCGAGACCTTTTTTGAtgttggc                                                   >  2:3091962/1‑148 (MQ=255)
                                                                                                 taaaCATGAGCACGTTTTTCCCCCTCCCCCTTCCCGGGTGTGGGTGGGGGTGTGGGCAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCTAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                                   <  1:490773/148‑1 (MQ=21)
                                                                                                     cATGCGCCCCGTTTTCCCCCGCTCCCTCCCGGGGAGCGGGTGGGGTTGCGTGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatca                                               <  1:2493365/150‑1 (MQ=25)
                                                                                                      aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                                              >  1:5782461/1‑150 (MQ=255)
                                                                                                        gAGCACCGTTTTCTCCCTCTCCCTTCCTGGTTGTGGGTGGGGTTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTc                                            <  2:565750/150‑1 (MQ=37)
                                                                                                         aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                                           >  2:2928362/1‑150 (MQ=255)
                                                                                                          gCACCTTTTTCTCCCTCCCCCTTCCTGGGTGTGGGTGGGGTTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                                          <  2:5276793/150‑1 (MQ=25)
                                                                                                           cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                                         >  1:882691/1‑150 (MQ=255)
                                                                                                             ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTATCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATAAGTCTGGaa                                       >  1:916509/1‑150 (MQ=255)
                                                                                                             ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                                       >  1:3821656/1‑150 (MQ=255)
                                                                                                             ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCACACCCAAAACCACTCCCCAAACGGGGCGAGGGGACAGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                                       >  1:1659680/1‑150 (MQ=255)
                                                                                                              cGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCTCACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAg                                      >  1:5968766/1‑150 (MQ=255)
                                                                                                                 tttCTCCCTCTCCACACCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                                   <  1:4826877/150‑1 (MQ=255)
                                                                                                                  ccgaccctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCCAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGATGTTGTCATCAGTCTGGAAGCCGc                                  >  2:4894668/5‑150 (MQ=25)
                                                                                                                   tctccctctcccTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa                                 >  2:4466488/1‑150 (MQ=255)
                                                                                                                   tctccctctcccTGCCAGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa                                 <  2:6160096/150‑1 (MQ=255)
                                                                                                                     tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCGGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg                               >  1:2712408/1‑150 (MQ=255)
                                                                                                                      ccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                              >  2:4841343/1‑150 (MQ=255)
                                                                                                                       cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGTCATCAGTCTGGAAGCCGCACggt                             >  1:4801143/1‑148 (MQ=255)
                                                                                                                       cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCGGGCCTGTTCCCCTCAACCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                             >  2:1966116/1‑150 (MQ=255)
                                                                                                                        ctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTAGGGAAGCCGCACGTTg                            >  1:2543112/1‑150 (MQ=37)
                                                                                                                         tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg                           >  2:4296352/1‑150 (MQ=255)
                                                                                                                         tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTACCCAAACGGGGCGAGGGGACTGAACGAGTCCTTTTTTTATGTTGTAATCAGTCTGGAATCCGCACGtttg                           >  1:1998860/1‑148 (MQ=25)
                                                                                                                          ctccctTCCCGGGAGAGGGTCGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc                          <  1:4535426/150‑1 (MQ=255)
                                                                                                                           tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                                         >  2:333867/1‑118 (MQ=255)
                                                                                                                           tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGaa                                       >  1:6709712/1‑136 (MQ=255)
                                                                                                                              ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATAAGTCTGGAAGCCGCACGTTGGCTTTa                      >  1:3458053/1‑150 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                                                <  1:4119537/123‑1 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                     >  2:14907/1‑150 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                     >  1:4222353/1‑150 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                     >  1:4349774/1‑150 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                     >  2:6728126/1‑150 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                     >  2:1801688/1‑150 (MQ=255)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCGCTCCCCCAACGGGGCGAGGGGCCTGACCGAGTCCTTGTTTGATGTTGTCCTCAGTCTGGAAGCCCCCCGGTGGCTTTAt                     >  1:2379683/1‑150 (MQ=37)
                                                                                                                               ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCCAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt                     >  1:1459749/1‑150 (MQ=255)
                                                                                                                                tCCTGGGAGTGGGTCGGGTTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtt                    <  2:4066649/150‑1 (MQ=255)
                                                                                                                                    ggttgagGGGGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                <  2:3559433/146‑1 (MQ=38)
                                                                                                                                    ggtatagGGTGGGGGTGTGTTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTa                <  2:4613155/145‑1 (MQ=38)
                                                                                                                                     ggAGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTAt               <  2:3826877/150‑1 (MQ=255)
                                                                                                                                        gagGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACCTTGGCTTTATTTTTAttta            >  1:1366438/1‑147 (MQ=255)
                                                                                                                                           ggTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCaaa         >  2:11504/1‑150 (MQ=255)
                                                                                                                                            gTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGTTGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATTTGaaat        >  1:4663294/1‑149 (MQ=255)
                                                                                                                                               ggggTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGaaa     >  2:5245813/1‑150 (MQ=255)
                                                                                                                                                  gTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                                                <  1:2838958/72‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTATGTCAAAGAAATGT  >  NC_000913/3239481‑3239776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: