Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,728,125 T→G 5.2% Y416S (TAC→TCC)  xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,728,1250TG 5.2% 880.8 / 6.9 306Y416S (TAC→TCC) xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base T (81/201);  new base G (20/2);  total (101/205)
Fisher's exact test for biased strand distribution p-value = 8.30e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  NC_000913/3727976‑3728274
                                                                                                                                                      |                                                                                                                                                     
gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGt                                                                                                                                                       >  2:1588886/1‑150 (MQ=255)
    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                   <  2:3865944/150‑1 (MQ=255)
    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                   <  2:4978416/150‑1 (MQ=255)
    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATc                                                                                                                                                   <  1:2579581/150‑1 (MQ=255)
     gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCGCCGCCGTGGGGTGATCg                                                                                                                                                  >  1:5045577/1‑150 (MQ=255)
     gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCGCCCCCGCCGGGCGGtgggcg                                                                                                                                                  >  1:1802863/1‑145 (MQ=255)
      cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGa                                                                                                                                                 <  2:5896890/150‑1 (MQ=255)
        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                               <  2:507508/150‑1 (MQ=255)
        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgc                                                                                                                                               <  2:519010/150‑1 (MQ=255)
        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAAGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCGCCCGTAGGGGAAGCGTgc                                                                                                                                               >  1:113198/1‑150 (MQ=255)
          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                             <  2:1368826/150‑1 (MQ=255)
          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAgctg                                                                                                                                             <  2:5556681/150‑1 (MQ=255)
              cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                                         <  2:3249886/150‑1 (MQ=255)
                cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGAcc                                                                                                                                       <  1:3387291/150‑1 (MQ=255)
                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                                      <  1:3958600/150‑1 (MQ=255)
                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                                      <  2:1164116/150‑1 (MQ=255)
                  gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAAGCGGGctgctgcgcgc                                                                                                                                     >  2:4149682/1‑145 (MQ=255)
                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                                    <  1:3094189/150‑1 (MQ=255)
                           gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTTCGGGCGTCGGGCGGCTGACCGCTGAGAGCCg                                                                                                                            >  2:6358407/1‑150 (MQ=255)
                            tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                           <  1:3806525/150‑1 (MQ=255)
                              gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                         <  1:1588886/150‑1 (MQ=255)
                               aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                        <  1:1329535/150‑1 (MQ=255)
                                tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCa                                                                                                                      >  1:5392532/1‑150 (MQ=255)
                                  cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                     <  2:3607313/150‑1 (MQ=255)
                                  cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                                     <  2:166761/150‑1 (MQ=255)
                                       gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGAc                                                                                                                <  2:5709533/150‑1 (MQ=255)
                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                                              <  1:482165/150‑1 (MQ=255)
                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                                              <  1:2219550/150‑1 (MQ=255)
                                           tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCTAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                            <  1:4041982/150‑1 (MQ=255)
                                           tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                                            <  2:2309820/150‑1 (MQ=255)
                                                gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                                                       <  2:5718617/150‑1 (MQ=255)
                                                   gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                    <  1:6395155/150‑1 (MQ=255)
                                                   gTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCAc                                                                                                    <  1:6559772/150‑1 (MQ=255)
                                                    ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTCCTCGAGCTGCTGACCGCTGGTGTCCGCCCGCCTCTGCCGCCCGTGCTCGCt                                                                                                   >  2:2369762/1‑150 (MQ=255)
                                                     tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                                  <  2:2679163/150‑1 (MQ=255)
                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGTACTCACTAc                                                                                                 >  2:336048/1‑150 (MQ=255)
                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACgcgc                                                                                             <  1:1006373/150‑1 (MQ=255)
                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAGCCCCCCCCGGGCGGTCCTGGGGCTGCTGGGGGCGGGTGGCCGCCCGCAGCGGACGCCGGGGCTCGCTCCgcgc                                                                                             >  1:2376901/1‑150 (MQ=255)
                                                           aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                                            <  2:6745526/150‑1 (MQ=255)
                                                            acaaTTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                                                           <  2:2504101/150‑1 (MQ=255)
                                                              aaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                                                         <  2:6067919/150‑1 (MQ=255)
                                                                ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                       <  1:1209007/150‑1 (MQ=255)
                                                                ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                                                      <  1:5589032/150‑1 (MQ=255)
                                                                  cAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                     <  2:1431410/150‑1 (MQ=255)
                                                                  cAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                     <  2:7234/150‑1 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGTCCCCGCCCGGACGGGGGGCGGGCTGCGGGCCGCTGGTCGCCGCCCGGGGCTGGCGCGCGTGCTCGCTCCGCGCCCCGccccc                                                                                    >  1:2781720/1‑150 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATGCCCCGCCGTGCGGGGTGCGTGCTGGTGACCGCTGTTGTCGGCCGGGGGCTGGCGCCAGGGGTCTCTGcgcgccccgcgccc                                                                                    >  1:5175119/1‑150 (MQ=255)
                                                                   aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGccccc                                                                                    <  1:6344987/150‑1 (MQ=255)
                                                                    aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGTCATCGCGCTGGTGACCGCTGGTCGCCGCCAGCGTCTGGGGCCCGTGCTCACTACGCGCCCCgccgccg                                                                                   >  2:2358071/1‑149 (MQ=255)
                                                                     tGAGCGATTTCTCTGGATTCGCCGCGAACTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGGACGGTAAGCGAGCTGCTGACCGCTGAGATCCCCCCCCCTCTGACGCCAGTACGCACGACGCGCCCCGccccccc                                                                                  >  2:6395569/1‑148 (MQ=255)
                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGGCAGCGGGCTGCTGGCCGCGGATGTCCGCCAGGCTCTGGCGCCAGTACTCTCTAGGCGCCCCGCGCCCAGTc                                                                                >  1:3459718/1‑150 (MQ=255)
                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                          <  1:1725462/138‑1 (MQ=255)
                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                          >  2:1725462/1‑138 (MQ=255)
                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCaa                                                                              <  1:359877/150‑1 (MQ=255)
                                                                              tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                                                         <  1:3274474/150‑1 (MQ=255)
                                                                                tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGGGCTGCTGACCGCTGATATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaca                                                                       >  2:4839811/1‑150 (MQ=255)
                                                                                tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAaca                                                                       <  1:4334150/150‑1 (MQ=255)
                                                                                  tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACt                                                                     <  2:1299642/150‑1 (MQ=255)
                                                                                       tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                                <  1:2664733/150‑1 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                             <  1:3666130/150‑1 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                             <  1:4513714/150‑1 (MQ=255)
                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                             <  1:4547623/150‑1 (MQ=255)
                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                                            <  1:983138/150‑1 (MQ=255)
                                                                                            gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTACGGGGGGCGCGGTGCTGACCGCTGATATCCGCCAGCCTCGGACGCCTGTACTCACTACGCGCCCCGCCCCCGCTCAACGTAACACGGTGCGGGTTa                                                           >  2:442517/1‑150 (MQ=255)
                                                                                             cgATCTGCGCCATCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGAGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                                                          <  1:153256/150‑1 (MQ=255)
                                                                                                tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCTCCCCCCCCGTGCGGTAATCGAGCTGCTGGCCGCTGCTCGCCGCCCGCGTCGGACGCCGGTCCGCGCTACGCGCCCCGCCCCCACTCGACGGACCCCGTTGCGGttgagtgc                                                       >  2:3229399/1‑144 (MQ=255)
                                                                                                tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                                                        >  2:6400864/1‑101 (MQ=255)
                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTTCGGTCATCGGGCTGCTGACCGCGGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTACCACTTTGCGGTGTAATGCCg                                                     >  2:3299622/1‑150 (MQ=255)
                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                                     <  1:6467768/150‑1 (MQ=255)
                                                                                                    cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGCAAACGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACCACGCGCCCCGCCCCCAATCAACCTAACACTTTGCGGTTTAATACCGCa                                                   <  2:2078964/150‑1 (MQ=255)
                                                                                                      ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAgg                                                 <  2:4334836/150‑1 (MQ=255)
                                                                                                      ccAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaa                                                 <  1:5312839/150‑3 (MQ=255)
                                                                                                           cTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                            <  2:2457283/150‑1 (MQ=255)
                                                                                                               ctgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa                                        <  2:4253100/150‑1 (MQ=255)
                                                                                                                tgcGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTCCCACTTTGCGGTTTAATACCGCAGGCATGCACGAc                                       >  2:4517596/1‑150 (MQ=255)
                                                                                                                 gcgcCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                                      <  2:6609500/150‑1 (MQ=255)
                                                                                                                    cccAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                                   >  2:5511415/1‑150 (MQ=255)
                                                                                                                      cAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                                 <  2:1978816/150‑1 (MQ=255)
                                                                                                                        gTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGc                               <  1:6643462/150‑1 (MQ=255)
                                                                                                                          gCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa                             <  2:2789761/150‑1 (MQ=255)
                                                                                                                           cTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt                            >  2:6675067/1‑150 (MQ=255)
                                                                                                                            tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                           >  2:2096264/1‑150 (MQ=255)
                                                                                                                            tGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTCGCGGTTTAATACCGCAGGCATGCACGACCTCCATGCCATc                           >  1:209816/1‑150 (MQ=255)
                                                                                                                              gCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg                         >  1:5677609/1‑150 (MQ=255)
                                                                                                                               ccccACATCCCCCCCCGTACGGTAATCGTGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGTCATGCACGTCATCCATGCCATCTGc                        <  2:4033535/150‑1 (MQ=255)
                                                                                                                                cccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACGCCATCCATGCCCTCTGcc                       >  2:1517194/1‑150 (MQ=255)
                                                                                                                                 ccACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATATGACGCCAg                                                                                                           >  2:3439594/1‑66 (MQ=255)
                                                                                                                                 ccACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGTTTAATACCGCAGGCATGCACGACATCCAAGCCATCTGCCa                      >  1:3715980/1‑150 (MQ=255)
                                                                                                                                  cacaTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGcccg                     >  2:6463155/1‑148 (MQ=255)
                                                                                                                                    caTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                   <  2:3718677/150‑1 (MQ=255)
                                                                                                                                    caTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg                   <  1:2347699/150‑1 (MQ=255)
                                                                                                                                     aTCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc                  <  1:4689146/150‑1 (MQ=255)
                                                                                                                                     aTCCCCCCCCGGACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcgc                  <  2:5259824/150‑1 (MQ=255)
                                                                                                                                         cccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATa               <  1:444942/150‑1 (MQ=255)
                                                                                                                                          ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              >  1:6199679/1‑150 (MQ=255)
                                                                                                                                          ccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa              <  2:6244798/150‑1 (MQ=255)
                                                                                                                                           cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc             >  1:1996540/1‑150 (MQ=255)
                                                                                                                                                gTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACg        <  2:1837414/150‑1 (MQ=255)
                                                                                                                                                     gTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTc   <  1:2393442/150‑1 (MQ=255)
                                                                                                                                                      tAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTcc  >  1:5908317/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGCCTTCC  >  NC_000913/3727976‑3728274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: