Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,501 T→A 8.4% intergenic (+56/+31) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5010TA 8.4% 896.9 / 9.6 291intergenic (+56/+31)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (81/174);  new base A (29/2);  total (112/179)
Fisher's exact test for biased strand distribution p-value = 1.56e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

AACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCT  >  NC_000913/3959353‑3959650
                                                                                                                                                    |                                                                                                                                                     
aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                                                                      >  1:6491093/1‑150 (MQ=255)
    caGCGATTCGCAGCCATGCGACTGAGCAGGTCGGTAAGAAACGGCGCGGCTCTATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACTCTCAAgg                                                                                                                                                  <  2:6722308/149‑1 (MQ=38)
    aaGCGATTCACAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                                                                  <  1:3521407/150‑1 (MQ=255)
     aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                                                 <  1:3079864/150‑1 (MQ=255)
     aGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                                                                 <  2:2759227/150‑1 (MQ=255)
         aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGCAAAAACTCAAGGCCTtc                                                                                                                                             >  1:3779153/1‑150 (MQ=255)
         aTTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtc                                                                                                                                             <  2:2172598/150‑1 (MQ=255)
           tCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTtctc                                                                                                                                           <  1:6042692/150‑1 (MQ=255)
             gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                         <  1:4956554/150‑1 (MQ=255)
              cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        >  2:1629346/1‑150 (MQ=255)
              cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        <  1:888908/150‑1 (MQ=255)
              cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        <  1:2606153/150‑1 (MQ=255)
              cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        <  2:6204051/150‑1 (MQ=255)
              cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACACCCACCGGGGGCGGGCGGAAACACACAAGGCCATCTccgg                                                                                                                                        >  1:5486531/1‑148 (MQ=255)
                 ccATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGgag                                                                                                                                     <  1:2660290/150‑1 (MQ=255)
                   aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                                                                   <  1:5300600/150‑1 (MQ=255)
                    tGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCAACCCCACCCACACCCACAGGGGGGGGGAGAAAAAACAAAAGGCCCTCTCCAggcggag                                                                                                                                  >  1:1736239/1‑145 (MQ=255)
                       gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                                                               <  1:2046371/150‑1 (MQ=255)
                        aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                              <  2:6053456/150‑1 (MQ=255)
                          tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGCGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                                            <  2:5160692/150‑1 (MQ=255)
                          tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                                            <  1:3466823/150‑1 (MQ=255)
                           gAGCAGGTAGGGAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGc                                                                                                                           <  1:2901496/150‑1 (MQ=255)
                             gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                                                                         <  1:2952176/150‑1 (MQ=255)
                             gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTa                                                                                                                         <  1:1418876/150‑1 (MQ=255)
                                  taggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACTCCCACAGGGAGAGGGAGAAAAAACTCAAGGCCATATACTGGAGAAGGCCTTGCGATTAGt                                                                                                                    >  2:768099/1‑150 (MQ=38)
                                     gtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACACCCACAGGGCGAGGGTGAAAACACTCAAAGCCATAAACAGGAGAAAGCCAGGCTATTAGTTgg                                                                                                                 >  1:568154/1‑149 (MQ=255)
                                      taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCTCTCCCACAGGGAGCGGGAGAAAACACTCAAGGCCATCTCCTGGAGAAGGCCGTGCTATTAGTTGCg                                                                                                                >  2:4517727/1‑150 (MQ=255)
                                       aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCGTCTCCTGGAGAACGCCGTGCTATTAGTTGCgg                                                                                                               >  2:3870766/1‑150 (MQ=255)
                                         gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGCCCCTCGACCCCACGGGGGGGGGGGAAACAAACAAGGCCGTGAGCCGGCGAAGGGCGTGCGATTAGGTTCgggt                                                                                                             >  2:2566537/1‑148 (MQ=255)
                                          aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTAc                                                                                                            <  2:4719497/150‑1 (MQ=255)
                                           aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                                                                           <  2:6677381/150‑1 (MQ=255)
                                           aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                                                                           >  2:1145037/1‑150 (MQ=255)
                                              tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACAACCCCACCCACAACCACACGGAGAGGGAGAAAAAACAAAAGGCCGTCTACAGGAGAACGCCTTGCTATTAGTTTAGGTACAGCa                                                                                                        >  1:4241438/1‑150 (MQ=255)
                                                 gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCTCTCCCACAGGGAGTGGGCGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCGTGCTATTAGTTGCGGTACAGCACCt                                                                                                     >  2:6139251/1‑150 (MQ=255)
                                                   ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCGACCCACACCCACACGCAGAGGGAGAAAAAACACAAAGGCATAAACAGGCGAAGGCCTTGGTATTAGTTGCGGTAAAGCACCATa                                                                                                   >  1:4020788/1‑150 (MQ=255)
                                                     cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCCACCCACTACCACAGGGAGAGGGAGAAAAAACGCAAGGCCATATACAGGAGAAGGCCGTGCTATTAGTTTCGGTGAAGCACCATaaa                                                                                                 >  2:3668867/1‑149 (MQ=25)
                                                      tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCGCAACCACAGGGGGAGGGAGAAAAAACACAAGGCCATATCCAGGAGAAGGCCGGGCTAATAGTTTAGGTACACCACCATaaat                                                                                                >  2:911447/1‑148 (MQ=255)
                                                         atGACAGATATGACACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                             <  1:923111/150‑1 (MQ=255)
                                                          tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                            <  1:1558841/150‑1 (MQ=255)
                                                          tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                            <  1:3176420/150‑1 (MQ=255)
                                                          tGACAGATATGAAACGTATTGCTGTTGCGGGGTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                                                                            <  2:3586915/150‑1 (MQ=255)
                                                           gACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCGCACCCCCACCCACACCCCCAGGGGGTGGGAGAAAAAAAACAAGGCCTTATCCTGGAGGAGGCCGTGCTATTAGTTTGGGTTCAGCACCATAATTAtttg                                                                                           >  2:2035305/1‑150 (MQ=255)
                                                                atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                      <  1:6289274/150‑1 (MQ=255)
                                                                atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATc                                                                                      <  1:635196/150‑1 (MQ=255)
                                                                  atGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCGACCCACAACCCCAGGGGGGGGGTGAAAAAACTCAAGGCCGTCAACCGGGGGAGGCCGTGCGATTAGTTGCGGTACAGCCCCATAATTAATTGATATCCg                                                                                    >  1:5523326/1‑150 (MQ=255)
                                                                    gAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCAACCAAAGGGAGAGGGAGAAAACAATCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGTTGTGATATCCGaa                                                                                  >  2:3423264/1‑150 (MQ=38)
                                                                        cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGCTGCCCTCACCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATGAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                              <  2:2873347/150‑1 (MQ=255)
                                                                        cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                                                              <  1:5762509/150‑1 (MQ=255)
                                                                        cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCACAGGGCGAGGGAGAAAAAACACAAGGCCATCTACAGGAGAAAGCCGTTCTAATATTTGCGGTACAACACCAAAAAGATGTGATATCCCaaaggg                                                                              >  2:5217656/1‑146 (MQ=255)
                                                                          tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                                                                            <  2:1406059/150‑1 (MQ=255)
                                                                            ttGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgtgt                                                                          <  1:1320624/150‑1 (MQ=255)
                                                                               cTGGTGCGGGTGAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCa                                                                       <  1:1021191/150‑1 (MQ=255)
                                                                                 gTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACTGGGAGAGGGAGAAATCACTCTAGGCCTTCTCCTGGATTAGGCCTTGCTATTATTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGc                                                                     <  1:3388546/150‑1 (MQ=38)
                                                                                  ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                                                                    <  2:5457663/150‑1 (MQ=255)
                                                                                  ttGCGGGTTAAGTGCGCGCTGATGCCCGAACCCCCACCCACACCCACCGGGGGCGGGAGAAAAAACAAAAGGCCATCTCCGGGAGAATGCCGTGCTATTAGTTGCGGTTCAGCACCATAATTTTGGGTTAAACGAAATGGGTGTGCcgcg                                                                    >  2:5790897/1‑150 (MQ=255)
                                                                                         ttAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGt                                                             <  1:2420727/150‑1 (MQ=255)
                                                                                            aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                          <  1:458688/150‑1 (MQ=255)
                                                                                              tGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCt                                                        <  2:1076407/150‑1 (MQ=255)
                                                                                                 gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                     <  1:1092037/150‑1 (MQ=255)
                                                                                                 gcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCa                                                     <  1:5152766/150‑1 (MQ=255)
                                                                                                  cgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCACGGCCGTCTCCTGGAGAAGGCCTTGCTATGAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAg                                                    <  2:1273487/150‑1 (MQ=255)
                                                                                                   gcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGt                                                   <  1:2281082/150‑1 (MQ=255)
                                                                                                      gATGCCCTCACCCCGACCCTCTCCCACCGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                <  2:411668/150‑1 (MQ=255)
                                                                                                            cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa                                          <  2:5052825/150‑1 (MQ=255)
                                                                                                             tCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGAACCGGACAag                                         >  2:1870415/1‑150 (MQ=255)
                                                                                                              cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                        <  2:1301380/150‑1 (MQ=255)
                                                                                                               aCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAagag                                       <  2:5269761/150‑1 (MQ=255)
                                                                                                                   cGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaaa                                   >  2:3390059/1‑150 (MQ=255)
                                                                                                                     aCCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAAcc                                 <  1:1384146/150‑1 (MQ=255)
                                                                                                                     aCCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTGGCTATTAGTTGCGGTACAGCACCTTAATGATGGGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGGACCGGACAAGAGAAAAcc                                 >  1:4101234/1‑150 (MQ=255)
                                                                                                                       ccTCTCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCTAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCAc                               >  1:609824/1‑150 (MQ=255)
                                                                                                                        ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                              >  1:324813/1‑150 (MQ=255)
                                                                                                                        ctctCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACt                              <  2:1719346/150‑1 (MQ=255)
                                                                                                                         tctcCCACAGGGAGAGGGAGAAAACACTCACGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                             <  2:3161528/150‑1 (MQ=255)
                                                                                                                         tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                             >  1:6424781/1‑150 (MQ=255)
                                                                                                                         tctcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACtt                             <  2:4325668/150‑1 (MQ=255)
                                                                                                                           tcCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTa                           <  2:801975/150‑1 (MQ=255)
                                                                                                                             ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         <  2:568154/150‑1 (MQ=255)
                                                                                                                             ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         >  2:1761754/1‑150 (MQ=255)
                                                                                                                             ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         <  2:2406676/150‑1 (MQ=255)
                                                                                                                             ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         <  2:110747/150‑1 (MQ=255)
                                                                                                                             ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         >  1:1876304/1‑150 (MQ=255)
                                                                                                                               acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa                       >  2:5249928/1‑150 (MQ=255)
                                                                                                                                   ggagagggagaAAACACACAAGGCCTTCTCCTGGAGAAGGCCTTGATATTAGTTGCGTTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGc                   <  1:3961219/150‑1 (MQ=255)
                                                                                                                                         ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTCTCGAACGCCGGAAc             >  2:4872725/1‑150 (MQ=255)
                                                                                                                                          gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAcc            >  2:1657906/1‑150 (MQ=255)
                                                                                                                                          gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACAGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAcc            >  1:6236960/1‑150 (MQ=255)
                                                                                                                                            gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                          >  2:1210431/1‑70 (MQ=255)
                                                                                                                                            gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                          <  1:1210431/70‑1 (MQ=255)
                                                                                                                                            gaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt          >  1:2760774/1‑150 (MQ=255)
                                                                                                                                             aaaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGACAACCACTGGATCGACCGCCGGAACCCTc         >  2:5985538/1‑150 (MQ=255)
                                                                                                                                              aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt        >  2:3081373/1‑150 (MQ=255)
                                                                                                                                              aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGGCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGACCCATCt        >  2:459107/1‑150 (MQ=255)
                                                                                                                                              aaaCACTCAAGGCCTTCGCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt        <  1:2171155/150‑1 (MQ=255)
                                                                                                                                               aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg       >  2:5556315/1‑150 (MQ=255)
                                                                                                                                               aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGAGATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACACGAGACAACCACTTTATCGAACGCCGGAACCATCTg       >  2:6424326/1‑150 (MQ=255)
                                                                                                                                                acacTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGa      <  1:3651570/150‑1 (MQ=255)
                                                                                                                                                    tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  2:4174599/1‑150 (MQ=255)
                                                                                                                                                    tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  2:3919618/1‑150 (MQ=255)
                                                                                                                                                    tCAAGGCCTTCTCCTGGAGAAGGCCATGCTATTAGTTGCGGTACAGCACCTTAATGATGTTATATCCGAACAGGGTGAGCAGCGGCCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  2:5530315/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCT  >  NC_000913/3959353‑3959650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: