Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 659,207 A→G 5.3% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913659,2070AG 5.3% 1274.3 / 38.0 378intergenic (+57/+44)tatE/lipATatABCE protein translocation system subunit/lipoate synthase
Reads supporting (aligned to +/- strand):  ref base A (195/162);  new base G (10/10);  total (205/173)
Fisher's exact test for biased strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01

Only 100 of 394 total aligned reads displayed.

GGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTC  >  NC_000913/659058‑659356
                                                                                                                                                     |                                                                                                                                                        
 ggTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCaaaa                                                                                                                                                         >  2:5993756/1‑150 (MQ=255)
 ggTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCaaaa                                                                                                                                                         >  2:1822411/1‑150 (MQ=255)
  gTTCAAGAAGGCGATGAATGATGACGATGCTGCGGAGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGTCGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCaaaaa                                                                                                                                                        <  2:5852521/150‑1 (MQ=255)
    tCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGc                                                                                                                                                      <  1:3680995/150‑1 (MQ=255)
      aagaagGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGAGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGTCGCTCCCTCAATATCTTGTTCGATACAAAAAAGCCGCTTCAAAAAGCgg                                                                                                                                                    >  1:5124670/1‑150 (MQ=255)
         aagGTGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGtt                                                                                                                                                 >  1:2732794/1‑150 (MQ=255)
          agGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATATAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                                                                                <  1:3374114/150‑1 (MQ=255)
          agGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATATAAAAAACCCGCTTTTTGAAGCGGGttt                                                                                                                                                <  1:3211429/150‑1 (MQ=255)
           ggCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGtttt                                                                                                                                               <  1:1134122/150‑1 (MQ=255)
             cGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGttttt‑‑t                                                                                                                                           <  1:5073395/150‑1 (MQ=255)
             cGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGttttt‑‑t                                                                                                                                           <  1:3795010/150‑1 (MQ=255)
               atgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TAt                                                                                                                                         >  1:2884880/1‑150 (MQ=255)
                tgaatgaTGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATc                                                                                                                                        <  1:237382/150‑1 (MQ=255)
                    tgatgaCGATTCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGAGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGAGGGTTTTT‑‑TATcagac                                                                                                                                    >  2:4118/1‑150 (MQ=255)
                     gatgaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATcagaca                                                                                                                                  <  1:1778344/150‑1 (MQ=255)
                     gatgaCGATGCTGCGGCGAAAAAAGGCGAAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCTATACAAAAAACCCGCTACAAAAAGCGGGTTTTT‑‑TATcagaca                                                                                                                                  >  2:4433100/1‑150 (MQ=255)
                      atgaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATcagacag                                                                                                                                 <  1:6363228/150‑1 (MQ=255)
                        gaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGAt                                                                                                                               >  1:5033830/1‑150 (MQ=255)
                        gaCGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGAt                                                                                                                               >  2:855510/1‑150 (MQ=255)
                                tgcGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAAACCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATgtaagtaa                                                                                                                       >  1:2432139/1‑150 (MQ=255)
                                  cggcgAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAatt                                                                                                                     >  2:4380598/1‑150 (MQ=255)
                                       aaaaaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTAc                                                                                                                >  2:6353922/1‑150 (MQ=255)
                                         aaaaGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAg                                                                                                              <  2:6729471/150‑1 (MQ=255)
                                            aGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGAt                                                                                                           >  1:905936/1‑150 (MQ=255)
                                               cgcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTAc                                                                                                        <  2:1123044/150‑1 (MQ=255)
                                                gcAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                                                                       <  1:3858576/150‑1 (MQ=255)
                                                  aGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTa                                                                                                     >  2:180364/1‑150 (MQ=255)
                                                      gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                                 <  1:1953414/150‑1 (MQ=255)
                                                      gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                                 >  2:3463023/1‑150 (MQ=255)
                                                      gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                                 <  1:3614627/150‑1 (MQ=255)
                                                      gTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACtt                                                                                                 <  2:4234396/150‑1 (MQ=255)
                                                        tGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTcc                                                                                               >  1:6591019/1‑150 (MQ=255)
                                                        tGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTcc                                                                                               <  1:6293476/150‑1 (MQ=255)
                                                         gATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCa                                                                                              >  1:1408498/1‑150 (MQ=255)
                                                            cTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCTATACAAAAAACCCGCTTCAAATAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGAtt                                                                                                          <  1:1809915/135‑1 (MQ=255)
                                                             ttCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATccc                                                                                          <  2:4875063/150‑1 (MQ=255)
                                                              tCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCt                                                                                         >  2:3143478/1‑150 (MQ=255)
                                                              tCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCt                                                                                         >  1:525119/1‑150 (MQ=255)
                                                                  gCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCg                                                                                     >  1:3308619/1‑150 (MQ=255)
                                                                  gCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCg                                                                                     >  1:4174641/1‑150 (MQ=255)
                                                                    tGAAAAGCTCTCTCATAAAGAGTGACGTCGCGAGCAGGACGCTCCATCAATATCATCTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTATTT‑‑TATCAGACAGATGTAAGTAAATAGTACAGGATTACTTAACTTCCATCCCTGTCGcc                                                                                   >  2:4159870/1‑150 (MQ=255)
                                                                     gAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCt                                                                                  >  1:4631316/1‑150 (MQ=255)
                                                                       aaaGATCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGc                                                                                >  1:2832103/1‑150 (MQ=255)
                                                                         aGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCaa                                                                              >  1:992357/1‑150 (MQ=255)
                                                                          gCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCaaa                                                                             >  2:2193988/1‑150 (MQ=255)
                                                                            tctctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATc                                                                           >  1:1026040/1‑150 (MQ=255)
                                                                            tctctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATc                                                                           <  2:256280/150‑1 (MQ=255)
                                                                              tctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCgg                                                                         >  1:5970447/1‑150 (MQ=255)
                                                                               ctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                                                                        <  1:443216/150‑1 (MQ=255)
                                                                               ctcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGc                                                                        <  2:1026040/150‑1 (MQ=255)
                                                                                tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                       >  2:4550664/1‑150 (MQ=255)
                                                                                tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                       >  2:3051904/1‑150 (MQ=255)
                                                                                tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                       >  1:4115648/1‑150 (MQ=255)
                                                                                tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                       >  1:571780/1‑150 (MQ=255)
                                                                                tcATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                       >  1:4297548/1‑150 (MQ=255)
                                                                                  aTAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTg                                                                     >  1:6501864/1‑150 (MQ=255)
                                                                                       gagTGACGTGGCGAGCAGGACGCTCCCTCAATCTCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaag                                                                <  2:3770478/150‑1 (MQ=255)
                                                                                         gTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTaagaa                                                              >  1:4684979/1‑150 (MQ=255)
                                                                                              gTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCg                                                         >  2:540136/1‑150 (MQ=255)
                                                                                               tGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCgg                                                        >  2:1388003/1‑150 (MQ=255)
                                                                                                ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                       >  1:3186484/1‑150 (MQ=255)
                                                                                                ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                       >  2:6556619/1‑150 (MQ=255)
                                                                                                ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                       >  1:1440633/1‑150 (MQ=255)
                                                                                                ggCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGa                                                       <  2:3288406/150‑1 (MQ=255)
                                                                                                      cAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACg                                                 <  2:2075932/150‑1 (MQ=255)
                                                                                                      cAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACg                                                 >  2:6460640/1‑150 (MQ=255)
                                                                                                         gACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTAATTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGAc                                              >  1:2077883/1‑150 (MQ=255)
                                                                                                          aCGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGAcc                                             <  2:5728202/150‑1 (MQ=255)
                                                                                                           cGCTCCCACAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGTTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCg                                            <  1:5882499/150‑1 (MQ=255)
                                                                                                            gCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGGGGTAAGAAGAGCGGACAAACGGACCGc                                           <  1:6310560/150‑1 (MQ=255)
                                                                                                              tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCAt                                         >  1:5491168/1‑150 (MQ=255)
                                                                                                              tCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCAt                                         >  1:2673410/1‑150 (MQ=255)
                                                                                                                 cTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgca                                      >  2:1589276/1‑150 (MQ=255)
                                                                                                                 cTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgca                                      >  1:4881199/1‑150 (MQ=255)
                                                                                                                  tCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgcag                                     >  1:3498983/1‑150 (MQ=255)
                                                                                                                    aaTATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGAGTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATgcagca                                   >  1:3662968/1‑150 (MQ=255)
                                                                                                                     atatCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCAt                                  <  2:1942466/150‑1 (MQ=255)
                                                                                                                       atCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATgg                                <  2:525119/150‑1 (MQ=255)
                                                                                                                           tGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCg                                                         <  2:2380090/121‑1 (MQ=255)
                                                                                                                           tGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTaa                            >  2:4807927/1‑150 (MQ=255)
                                                                                                                            gTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTaaa                           <  2:6303173/150‑1 (MQ=255)
                                                                                                                             ttCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTACTTCTTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACACACGGACCGCATGCAGCATGGGTAAAg                          >  2:5557564/1‑150 (MQ=255)
                                                                                                                                gATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACt                                                                                                       <  2:6190115/70‑1 (MQ=255)
                                                                                                                                gATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGccc                       >  2:3877715/1‑150 (MQ=255)
                                                                                                                                 aTACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCa                      >  1:4790678/1‑150 (MQ=255)
                                                                                                                                      aaaaaCCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCAt                                         <  2:6150776/126‑1 (MQ=255)
                                                                                                                                      aaaaaCCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGcc                 >  2:3219517/1‑150 (MQ=255)
                                                                                                                                       aaaaCCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCa                <  1:6106427/150‑1 (MQ=255)
                                                                                                                                        aaaCCCGCTTTTTGAAGCGGGTTTTTTGTATCGAACAAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGc                  >  2:5577825/1‑150 (MQ=37)
                                                                                                                                        aaaCCCGCTTTTTGAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCg                                                                       >  1:778178/1‑94 (MQ=38)
                                                                                                                                         aaCCCGCTTTTTGAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGc                                                                                <  2:5097858/84‑1 (MQ=38)
                                                                                                                                         aaCCCGCTTTTTGAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGc                                                                                >  1:5097858/1‑84 (MQ=38)
                                                                                                                                           cccGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAgcgc            <  1:4258939/150‑1 (MQ=255)
                                                                                                                                             cGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCtt          >  2:6068092/1‑150 (MQ=255)
                                                                                                                                              gCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTc         >  2:459348/1‑150 (MQ=255)
                                                                                                                                                ttCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCgg       <  2:4308692/150‑1 (MQ=255)
                                                                                                                                                   aaaaaGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCtt    >  1:1291871/1‑150 (MQ=255)
                                                                                                                                                   aaaaaGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCtt    >  1:3086521/1‑150 (MQ=255)
                                                                                                                                                    aaaaGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCttt   <  2:5437747/150‑1 (MQ=255)
                                                                                                                                                    aaaaGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCttt   >  2:2769644/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
GGGTTCAAGAAGGCGATGAATGATGACGATGCTGCGGCGAAAAAAGGCGCAGACGTTGATCTTCAGGCTGAAAAGCTCTCTCATAAAGAGTGACGTGGCGAGCAGGACGCTCCCTCAATATCTTGTTCGATACAAAAAACCCGCTTCAAAAAGCGGGTTTTT‑‑TATCAGACAGATGTAAGTAATTATTACAGGATTACTTAACTTCCATCCCTTTCGCCTGCAAATCGGCGTGGTAAGAAGAGCGGACAAACGGACCGCATGCAGCATGGGTAAAGCCCATCGCCAGCGCTTCGGCTTTC  >  NC_000913/659058‑659356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: