Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 843,216 G→T 14.6% M295I (ATG→ATT rlmF → 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913843,2160GT14.6% 697.0 / 25.6 252M295I (ATG→ATTrlmF23S rRNA m(6)A1618 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (79/129);  new base T (41/2);  total (121/131)
Fisher's exact test for biased strand distribution p-value = 3.65e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

CAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTT  >  NC_000913/843067‑843365
                                                                                                                                                     |                                                                                                                                                      
cAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATg                                                                                                                                                        >  2:3384916/1‑150 (MQ=255)
  ggTGATTTGGTTTACATCACTGGTATCTCGTGGTGAAATATTACCGCCGTTGTATCGTGCCCTGACGGTCGTGGGCGCGGTGAAGGTGGTTAAAAAATAGATTGCCCAGTGTCAAAAGCAGAGTCGCTTTATTTCCTGGACCTTTATGaa                                                                                                                                                      <  1:6044343/150‑1 (MQ=255)
        gtgGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAAcgacga                                                                                                                                                <  2:2846696/150‑1 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTTTTTCCTGGGCCGTTATGAAAGACGagca                                                                                                                                             >  2:1755217/1‑150 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagca                                                                                                                                             >  2:407614/1‑150 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagca                                                                                                                                             <  2:3925967/150‑1 (MQ=255)
           gTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCATTATTAACGACGagca                                                                                                                                             >  2:3784152/1‑150 (MQ=255)
            tttACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCTGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagcag                                                                                                                                            >  2:3589882/1‑150 (MQ=255)
             ttACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagcagc                                                                                                                                           <  1:1404272/150‑1 (MQ=255)
             gtACATCACTGGTATCTCGTGGTGAACACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGagcagc                                                                                                                                           <  2:5596875/149‑1 (MQ=255)
                 aTCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGGCTGGACCGTTATTTACGAAGAGCAGcgccg                                                                                                                                       >  1:6654079/1‑150 (MQ=255)
                  tCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGTTGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGcgccgc                                                                                                                                      <  1:3769037/150‑1 (MQ=255)
                  tCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCAGGAGCTTTTTTAAAGACGAGCAGcgccgc                                                                                                                                      >  1:4856860/1‑150 (MQ=255)
                   cACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGAGGTGGGCGCGGTGAAGGTGTTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCgccgcc                                                                                                                                     <  1:4690242/150‑1 (MQ=255)
                      tGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCAGGACCGTTATGAACGACGAGCAGCGCCGCCCtt                                                                                                                                  >  1:572269/1‑150 (MQ=255)
                       ggTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGttt                                                                                                                                 <  2:5953241/150‑1 (MQ=255)
                        ggctctcGTGGGGACCACTTCCCGCCGTTGTATCGGGCCCGGACGGACGTGGGCGCGGTGAAGGTGGTTAAACAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGtttt                                                                                                                                <  2:4256561/147‑1 (MQ=255)
                        gTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGTACCTTTATTAACGACGAGCAGCGCCGCCGtttt                                                                                                                                <  1:3921720/150‑1 (MQ=255)
                        gTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGtttt                                                                                                                                <  1:2107309/150‑1 (MQ=255)
                        gTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGtttt                                                                                                                                <  1:6531911/150‑1 (MQ=255)
                        gTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGtttt                                                                                                                                <  2:1551274/150‑1 (MQ=255)
                          aTCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGTGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGt                                                                                                                              <  1:4742981/150‑1 (MQ=255)
                             tcGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCaa                                                                                                                           <  1:2807277/150‑1 (MQ=255)
                             tcGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCaa                                                                                                                           <  1:5063743/150‑1 (MQ=255)
                              cGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAAt                                                                                                                          <  1:3411853/150‑1 (MQ=255)
                              cGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCGGTAATAACGACCAGCAGCGCCGCCGGTTTGTCacg                                                                                                                          >  2:3265751/1‑148 (MQ=255)
                               gtggtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTAATTGACCGTTATTAAAAACGAGAAGCGCCCCCCTTTTGTCaaaa                                                                                                                         >  1:1210657/1‑148 (MQ=255)
                               gtggtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATc                                                                                                                         <  2:4313373/150‑1 (MQ=255)
                                  gtgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCTGGACCATTATAAAAGACGATCAGCGCCGCCCTTTTTTAAATCGcc                                                                                                                      >  2:5643807/1‑150 (MQ=255)
                                   tgAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCGTTATGAAAGACGAGCAGCGCCGCCGTTTTGTCAATCGccc                                                                                                                     >  2:2694926/1‑149 (MQ=255)
                                      aaaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTCCTGGGCCGTTATTTAACACCAGCAGCCCCCCCCGTTTTTCAAACGCCACCg                                                                                                                  >  1:5504719/1‑150 (MQ=255)
                                       aaCTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGTCCATTATGAACCACGAGCAGCGCCGCCGGTTTGTCAATAGCCAGCgg                                                                                                                 >  2:2558697/1‑149 (MQ=255)
                                         cTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCGGGACCATTATTAAAGACGAGCACCGCCGCCGGTTTTTAAAAAGCCACCGTTa                                                                                                               >  2:1408602/1‑150 (MQ=255)
                                           tACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTaaa                                                                                                             <  2:2157995/150‑1 (MQ=255)
                                            aCCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCCGGACCATTATGAACGCCGCGCAGCGCCGCCCGTTTGTCAAACGCCAGCGGTAAAg                                                                                                            >  2:2722807/1‑150 (MQ=255)
                                             ccgccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGc                                                                                                           <  1:2586727/150‑1 (MQ=255)
                                              cgccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCGGGACCATTTTTTAAGACCAGCACCGCCGCCCGTTTGTCAAACGCCCGCCGTaaaaag                                                                                                          >  1:5918695/1‑147 (MQ=255)
                                               gccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGAAGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCAGGACCAGTATGAACCAAGAGCACCGCACCCGCTTTGACAATCCCCAACGTTAAAGCGt                                                                                                        >  2:6773418/1‑150 (MQ=255)
                                                ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                                       <  1:725956/150‑1 (MQ=255)
                                                ccgTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGtt                                                                                                       <  2:1086260/150‑1 (MQ=255)
                                                  gTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTTTTGCCTGGACCTTTAATAACGACGAGCAGCCCCGCCGGTTTGTCAATCGCCAGCGGTAAAGCGTTgg                                                                                                     >  2:1453977/1‑150 (MQ=255)
                                                         cGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTccccc                                                                                              <  1:1560194/150‑1 (MQ=255)
                                                           tGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcgg                                                                                            <  2:5757211/150‑1 (MQ=255)
                                                           tGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcgg                                                                                            <  1:1408602/150‑1 (MQ=255)
                                                           tGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcgg                                                                                            <  2:5278158/150‑1 (MQ=255)
                                                              ccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGGCCTTTATGAACGACGAGCCGCGCCGCCGGTTTGTCAAACGCCAGCGGTAAATCGTTGGTTCCCCccgcgg                                                                                         >  1:4975917/1‑150 (MQ=255)
                                                              ccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCcggcgg                                                                                         >  2:6171845/1‑150 (MQ=255)
                                                              ccTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGGTTTTTGCTGGGCCGTTATTAAAGACCAGCACCCCCCCCCGTTTTTGAATAGCCCCCGGTAAAGCGGTGGTTGCCCccgcgg                                                                                         >  1:3184412/1‑150 (MQ=255)
                                                                tGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCTGGACCGTTATTAAAGACGAGCAGCGCCGCCGTTTTTTGAATAGCCACCGGTAAAGCGTTGGTTGCCCccgcggga                                                                                       >  1:1637652/1‑148 (MQ=255)
                                                                 gacggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCGGGGCCATTATTAAAGACCAGCAGCGCCGCCCGGTTTTGAAACGCCACCGGTTAAGCGTTTGTTTCCCCcgccggaa                                                                                       >  1:6539084/1‑148 (MQ=255)
                                                                   cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACg                                                                                    <  1:1025269/150‑1 (MQ=255)
                                                                   cggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACg                                                                                    <  1:2205874/150‑1 (MQ=255)
                                                                    ggacgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACgg                                                                                   <  1:5020080/150‑1 (MQ=255)
                                                                      acgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGtt                                                                                 <  1:2438788/150‑1 (MQ=255)
                                                                      acgTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGtt                                                                                 <  2:4746789/150‑1 (MQ=255)
                                                                        gTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCa                                                                               <  1:3514351/150‑1 (MQ=255)
                                                                             cgcgGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCgg                                                                          <  1:1821427/150‑1 (MQ=255)
                                                                             cgcgGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCgg                                                                          <  1:3100841/150‑1 (MQ=255)
                                                                              gcgGTGAAGGTTGTTAAAAAAGAGAGGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGCCCGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTGGGTTCCCCCGGCGGTAACGGTTCAGCCggg                                                                         <  2:4030279/150‑1 (MQ=255)
                                                                                      ggtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTTTTTGCGGGGCCATTATTAAAGACGAGCAGCCCCCCCCGTTTTTGAAAAGCCACCGGTAAAACGTTGGTTCCCCCCGCGGGAAACGTTCAACCGGGGTGGCGcc                                                                 >  1:4461309/1‑150 (MQ=255)
                                                                                       gtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCGTTATTAAAGACGAGCAGCGCCGCCGTTTTGTCAAAAGCCAGCGTTAAATCGTTTGTTACCCCCGCGGTAAACGTTCAGCCGGGGTCGCGccc                                                                >  2:848680/1‑150 (MQ=255)
                                                                                       gtggtTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCGGGACCATTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGccc                                                                >  2:4533973/1‑150 (MQ=255)
                                                                                            tAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgc                                                           <  2:5918695/150‑1 (MQ=255)
                                                                                             aaaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgca                                                          <  2:500924/150‑1 (MQ=255)
                                                                                             aaaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCGTTATTTGCGGGACCGTTATTAAAGAAGAGCAGCGCCGCCCGTTTGTGAAAAGCCACCCGTTAAGCGGTGGTTGCCCCCGCCGGAAAGGTTCAGCCGGGGTGGCGCCCGGAgca                                                          >  2:2352271/1‑150 (MQ=255)
                                                                                              aaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAgcag                                                         <  2:637245/150‑1 (MQ=255)
                                                                                              aaaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGGTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCCGGGTCGCGCCCGGAgcag                                                         >  2:1692308/1‑150 (MQ=255)
                                                                                               aaaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTCCTGGACCTTTATGAAAGACGAGCAGCGCCGCCCTTTTGTCAAAAGCCACCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTAAGCCGGGGTCGCGCCCGGAgcagg                                                        >  2:2770419/1‑150 (MQ=255)
                                                                                                aaaGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTTGCGGGACCTTTATTAAAGACGAGCAGCGCCCCCGTTTTTTAAAAAGCCAGCGTTAAAGCGTTGGTTCCCCCCGCGGTAACCGTTCAGCCGGGGTCGCGCCCCGAgcaggc                                                       >  2:2983116/1‑150 (MQ=255)
                                                                                                      aTGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCAcc                                                 <  1:900978/150‑1 (MQ=255)
                                                                                                      aTGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCAcc                                                 <  2:5729729/150‑1 (MQ=255)
                                                                                                       tGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGGTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCt                                                <  2:3811293/150‑1 (MQ=255)
                                                                                                          cccAGGGGCAAAAGCAGAGTCGCTTTTTTGCCTGGACCTTTTTGAACGACGAGCCGCGCCGCCCTTTTGTCCATCGCCAGCGGTAAAGCGTTTGGTCCCCCGGCGGTAACGGGTCAGCCGGGGTCGCGCCCGGCGCAGGCCGCACCGGAt                                             >  2:2636541/1‑150 (MQ=255)
                                                                                                           ccAGGGGCAAAAGCAGAGTCGCTTTTTTGCCGGGACCTTTATGAACGCCGCGCAGCGCCGCCGGTGTGTCAATCGCCAGCGGTAAAGCGTTGGTTCCCCCGGCGGGAACGGTGCCGCCGGGGGCGCGCCCGGGGCCGGCGGccccggcta                                            >  2:3690185/1‑145 (MQ=255)
                                                                                                            cAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                                           <  2:1637652/150‑1 (MQ=255)
                                                                                                            cAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATaa                                           <  2:5471761/150‑1 (MQ=255)
                                                                                                                 gCAAACGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTg                                      <  2:6689651/150‑1 (MQ=255)
                                                                                                                   aaaaGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCa                                    <  1:5127318/150‑1 (MQ=255)
                                                                                                                    aaaGCAGAGTCGCTTTATTGGCGGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAAACTCCACCGTTAAATCGCAGGTTAACCCCGCGGTAACGGTTAAGCCCGGGTCGCGCCCCGAGCAGGCAGCACCAGATAATTATGCaa                                   >  2:1945429/1‑149 (MQ=255)
                                                                                                                      aGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTg                                 <  2:1809475/150‑1 (MQ=255)
                                                                                                                        cAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGgc                               <  2:5093855/150‑1 (MQ=255)
                                                                                                                             tCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGGTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGcgcgcg                          >  1:6753870/1‑150 (MQ=255)
                                                                                                                                cTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAc                       >  1:6227574/1‑150 (MQ=255)
                                                                                                                                 tttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcg                      >  2:3064167/1‑150 (MQ=255)
                                                                                                                                  ttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCTGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgc                     >  1:3686999/1‑150 (MQ=255)
                                                                                                                                  ttATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgc                     <  2:4547574/150‑1 (MQ=255)
                                                                                                                                    aTTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTTTAAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTTATATGTCTGCACTGGCGCGCGAAcgccc                   <  1:407614/150‑1 (MQ=255)
                                                                                                                                    aTTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGTTTCAGCCGGGGTCGCGCCCGGAGCTGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgccc                   <  1:5633045/150‑1 (MQ=255)
                                                                                                                                    aTTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgccc                   <  1:6085032/150‑1 (MQ=255)
                                                                                                                                     ttGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGa                                                             >  2:203977/1‑107 (MQ=255)
                                                                                                                                     ttGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAAcgcccg                  >  1:3867958/1‑150 (MQ=255)
                                                                                                                                        ccTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCa               <  2:3184412/150‑1 (MQ=255)
                                                                                                                                         cTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGATAAAGCGTTGGTTCCCCCGGAGGTAACGGTTCATCCGTGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCTCGCGAACGCCCGCCAg              >  2:4160858/1‑150 (MQ=255)
                                                                                                                                           ggACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGGGCGTTCGCGCGCCAgtg            <  2:2263075/150‑3 (MQ=255)
                                                                                                                                               cTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGaa        >  2:4707165/1‑150 (MQ=255)
                                                                                                                                               cTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGaa        >  2:4444588/1‑150 (MQ=255)
                                                                                                                                                  tATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATg     >  1:994334/1‑150 (MQ=255)
                                                                                                                                                   aTGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGt    >  1:3314955/1‑150 (MQ=255)
                                                                                                                                                     gAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGttt  <  1:6171845/150‑1 (MQ=255)
                                                                                                                                                     gAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGttt  >  2:609201/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CAGGTGATGTGGTTTACATCACTGGTATCTCGTGGTGAAAACTTACCGCCGTTGTATCGTGCCCTGACGGACGTGGGCGCGGTGAAGGTGGTTAAAAAAGAGATGGCCCAGGGGCAAAAGCAGAGTCGCTTTATTGCCTGGACCTTTATGAACGACGAGCAGCGCCGCCGTTTTGTCAATCGCCAGCGTTAAAGCGTTGGTTCCCCCGGCGGTAACGGTTCAGCCGGGGTCGCGCCCGGAGCAGGCAGCACCTGATAAGTCTGCACTGGCGCGCGAACGCCCGCCAGGTCGAAATGTTT  >  NC_000913/843067‑843365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: