Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,258 G→T 5.2% intergenic (+24/+19) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2580GT 5.2% 1397.2 / 37.8 387intergenic (+24/+19)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (173/193);  new base T (11/10);  total (184/203)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 393 total aligned reads displayed.

CTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCA  >  NC_000913/993109‑993407
                                                                                                                                                     |                                                                                                                                                     
    ttCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACTGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgcc                                                                                                                                                   <  1:2548403/150‑1 (MQ=255)
    ttCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgcc                                                                                                                                                   <  2:2380288/150‑1 (MQ=255)
    gtCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgcc                                                                                                                                                   <  1:6045580/149‑1 (MQ=255)
     tCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGcgccg                                                                                                                                                  >  2:5128515/1‑150 (MQ=255)
       gCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCgccgcc                                                                                                                                                >  1:910661/1‑150 (MQ=255)
        ccTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCa                                                                                                                                               >  1:1905840/1‑150 (MQ=255)
          tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCAtt                                                                                                                                             <  1:2307275/150‑1 (MQ=255)
           gAAACGTTACGATGCCAAACGTCTGGAGAAATTGCGGGGGGCGCTGGAACTGTTGAATGGGCTGGAAAATCTCTCTGGCTATCTGTACGAGATTATAACTAATGCACTGGATTGATAAATAACGGATTGGCGGCGCTATTGCCGCCATTc                                                                                                                                            <  1:1133194/150‑1 (MQ=255)
           gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                            <  2:2081748/150‑1 (MQ=255)
            aaaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCg                                                                                                                                           >  2:3031308/1‑150 (MQ=255)
             aaCGTTACGATGCCACACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCgg                                                                                                                                          <  2:1518003/150‑1 (MQ=255)
             aaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCgg                                                                                                                                          <  2:1452802/150‑1 (MQ=255)
              aCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGACTGGCGGCAATAGCGCCGCCATTCggg                                                                                                                                         <  2:6382375/150‑1 (MQ=255)
                gTTCCGAGGCCCAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCCGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGa                                                                                                                                       <  2:5378273/150‑1 (MQ=255)
                  tACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAAt                                                                                                                                     >  1:805851/1‑150 (MQ=255)
                      aTGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAc                                                                                                                                 <  2:699673/150‑1 (MQ=255)
                      aTGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAc                                                                                                                                 <  2:2635413/150‑1 (MQ=255)
                       tGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcc                                                                                                                                <  2:2797906/150‑1 (MQ=255)
                        gCCAAACGTCAGGAGAAATTGCGCGCGGCGCTGGAAAAGTTGAATGGGCTGGAAAATCTCGCTGGCGATCTGTACTAGAAGATAACTAAACCAGTGGCTTGATAAATAACCGAATGGCGGAATTAGCGCCGCCATTCGGGGAATTTAccc                                                                                                                               <  1:3252968/150‑1 (MQ=255)
                        gCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAccc                                                                                                                               <  2:6554245/150‑1 (MQ=255)
                        gCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAccc                                                                                                                               >  1:2162198/1‑150 (MQ=255)
                         ccAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTAcccc                                                                                                                              >  2:2833625/1‑150 (MQ=255)
                          cAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCt                                                                                                                             >  2:2713504/1‑150 (MQ=255)
                                   ggAGAAAATGCGCGCGGCGCTGGAACTGTTGAATGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                    <  1:5051106/150‑1 (MQ=255)
                                   ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                    >  1:2708193/1‑150 (MQ=255)
                                   ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                    <  2:2782257/150‑1 (MQ=255)
                                    gagaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtctctg                                                                                                                   >  1:4199970/1‑148 (MQ=255)
                                    gagaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAg                                                                                                                   >  2:6093072/1‑150 (MQ=255)
                                     agaAATTGCGCGCGGCTCTGGAACAGTTGAAAGGGCTGTAAAATCTCTCTGGCGATCTTTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAgg                                                                                                                  <  1:349953/150‑1 (MQ=255)
                                      gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                                 <  2:337778/150‑1 (MQ=255)
                                      gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                                 >  2:4995045/1‑150 (MQ=255)
                                       aaaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCg                                                                                                                >  2:926587/1‑150 (MQ=255)
                                       aaaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCg                                                                                                                >  1:2690831/1‑150 (MQ=255)
                                        aaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGa                                                                                                               >  1:4375304/1‑150 (MQ=255)
                                            gcgcgcGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGttt                                                                                                           <  1:5600194/150‑1 (MQ=255)
                                                gcggcgCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAg                                                                                                        >  1:4893841/1‑149 (MQ=255)
                                                gcggcgCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGa                                                                                                       <  1:5206718/150‑1 (MQ=255)
                                                   gcgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTc                                                                                                    <  1:4159076/150‑1 (MQ=255)
                                                    cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                                   <  2:1890859/150‑1 (MQ=255)
                                                       tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGACTTTACCCCTGTTTTCTCAGGCGAGTGTCAGATTcacca                                                                                                >  2:57210/1‑150 (MQ=255)
                                                           aCAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGtt                                                                                            <  1:3136202/150‑1 (MQ=255)
                                                             aGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGc                                                                                          <  1:6159907/150‑1 (MQ=255)
                                                              gTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCa                                                                                         <  2:1603813/150‑1 (MQ=255)
                                                               ttGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCAt                                                                                        >  2:4578944/1‑150 (MQ=255)
                                                                 gAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCa                                                                                      >  1:1320210/1‑150 (MQ=255)
                                                                  aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGACGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAc                                                                                     <  2:4753343/150‑1 (MQ=255)
                                                                  aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                                   <  1:1060783/136‑1 (MQ=255)
                                                                  aaaGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                                   >  2:1060783/1‑136 (MQ=255)
                                                                    aGGGCTGGAAAATCTCTCTGGCGATCTGTATGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCAccc                                                                                   >  2:5944097/1‑150 (MQ=255)
                                                                       gCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                                                                                <  2:1201679/150‑1 (MQ=255)
                                                                       gCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                                                                                <  2:4248330/150‑1 (MQ=255)
                                                                         tGGAAAATCTCTCTGGCGATCTGTACGATAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGACTTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                              >  2:5849364/1‑150 (MQ=255)
                                                                         tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                              >  1:2886177/1‑150 (MQ=255)
                                                                           gAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCac                                                                            >  1:5831532/1‑149 (MQ=255)
                                                                           gAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCaa                                                                            >  1:446765/1‑150 (MQ=255)
                                                                           gAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCaa                                                                            <  1:4679657/150‑1 (MQ=255)
                                                                                tctctcTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCt                                                                       >  1:3822812/1‑150 (MQ=255)
                                                                                  tctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc                                                                     <  2:4355401/150‑1 (MQ=255)
                                                                                  tctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc                                                                     <  1:5436661/150‑1 (MQ=255)
                                                                                    tctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGc                                                                   >  2:1393161/1‑150 (MQ=255)
                                                                                    tctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGc                                                                   >  1:6327350/1‑150 (MQ=255)
                                                                                                tACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGc                                                       >  2:1008424/1‑150 (MQ=255)
                                                                                                     gaagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATc                                                  <  2:6040711/150‑1 (MQ=255)
                                                                                                      aagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCg                                                 <  1:1008424/150‑1 (MQ=255)
                                                                                                            aCTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGa                                           <  1:5268545/150‑1 (MQ=255)
                                                                                                              tAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAcc                                         >  1:4259837/1‑150 (MQ=255)
                                                                                                              tAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAcc                                         >  2:1841033/1‑150 (MQ=255)
                                                                                                                 aGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTa                                      <  2:214982/150‑1 (MQ=255)
                                                                                                                    aCTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGTAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATc                                   <  1:1793793/150‑1 (MQ=255)
                                                                                                                    aCTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATc                                   <  1:3604937/150‑1 (MQ=255)
                                                                                                                      tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                                 >  2:3676483/1‑150 (MQ=255)
                                                                                                                      tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                                 >  1:3207388/1‑150 (MQ=255)
                                                                                                                      tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                                 >  1:6272705/1‑150 (MQ=255)
                                                                                                                      tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                                 <  2:2346552/150‑1 (MQ=255)
                                                                                                                      tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                                 <  1:1393161/150‑1 (MQ=255)
                                                                                                                      tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTGTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                                 >  2:4866336/1‑150 (MQ=255)
                                                                                                                       ggCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAc                                <  2:2180343/150‑1 (MQ=255)
                                                                                                                          ttGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                    >  2:3450588/1‑63 (MQ=255)
                                                                                                                          ttGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                                    <  1:3450588/63‑1 (MQ=255)
                                                                                                                             ataaataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGAc                          >  2:5072902/1‑150 (MQ=255)
                                                                                                                                 ataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACgggg                      <  2:4394732/150‑1 (MQ=255)
                                                                                                                                 ataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACgggg                      >  1:148536/1‑150 (MQ=255)
                                                                                                                                      cGAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGatat                 >  2:2928174/1‑150 (MQ=255)
                                                                                                                                      cGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGatat                 <  1:480310/150‑1 (MQ=255)
                                                                                                                                      cGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGatat                 >  2:3911801/1‑150 (MQ=255)
                                                                                                                                       gAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCAGTTTTCTCAGGCGAGTTTCAGATTAACCACCTTGCATCACCCCTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc                >  1:2660648/1‑150 (MQ=255)
                                                                                                                                       gAATGGCGGCGCTATTGCCGCCATTCGGGGAATTTAACCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACCGGGGATATc                >  1:641574/1‑150 (MQ=255)
                                                                                                                                       gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc                >  2:4690459/1‑150 (MQ=255)
                                                                                                                                       gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc                >  2:3247997/1‑150 (MQ=255)
                                                                                                                                        aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc               >  2:3773740/1‑150 (MQ=255)
                                                                                                                                        aaTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATcc               >  2:2434284/1‑150 (MQ=255)
                                                                                                                                         aTGGCGGCGCTATTGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCa              >  2:5506150/1‑150 (MQ=255)
                                                                                                                                           ggcggcAATCGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACt            >  2:63627/1‑150 (MQ=255)
                                                                                                                                            gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTg           >  2:527260/1‑150 (MQ=255)
                                                                                                                                            gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTg           >  1:2482333/1‑150 (MQ=255)
                                                                                                                                               gcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCa        >  1:6673411/1‑150 (MQ=255)
                                                                                                                                                 aaTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCaaa      <  2:2011711/150‑1 (MQ=255)
                                                                                                                                                  aTAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAAt     <  1:2411028/150‑1 (MQ=255)
                                                                                                                                                   tAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTAAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATc    <  1:3989088/150‑1 (MQ=255)
                                                                                                                                                     gcgcCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCa  <  2:2558833/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTCAAATCCA  >  NC_000913/993109‑993407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: