Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,229 G→T 5.3% intergenic (‑25/+283) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2290GT 5.3% 925.9 / 3.0 307intergenic (‑25/+283)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (152/125);  new base T (3/26);  total (155/152)
Fisher's exact test for biased strand distribution p-value = 2.56e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

TGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGA  >  NC_000913/2177080‑2177378
                                                                                                                                                     |                                                                                                                                                     
tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCg                                                                                                                                                       >  2:4278526/1‑150 (MQ=255)
tGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTCg                                                                                                                                                       >  1:4683380/1‑150 (MQ=255)
 gCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGa                                                                                                                                                      <  2:6117787/150‑1 (MQ=255)
   aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                    <  1:2560581/150‑1 (MQ=255)
   aGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAtt                                                                                                                                                    >  2:4197226/1‑150 (MQ=255)
     gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTc                                                                                                                                                  >  1:4815929/1‑150 (MQ=255)
      ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                 <  1:5271768/150‑1 (MQ=255)
       tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                <  1:5641430/150‑1 (MQ=255)
           aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAata                                                                                                                                            >  2:5917308/1‑150 (MQ=255)
            gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                           <  2:5551656/150‑1 (MQ=255)
             cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                          <  1:1865599/150‑1 (MQ=255)
                      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                 <  2:454884/150‑1 (MQ=255)
                         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                              >  1:4410335/1‑150 (MQ=255)
                           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                            <  2:17145/150‑1 (MQ=255)
                           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                            <  2:4318170/150‑1 (MQ=255)
                           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTAAAAATATAATGAAAATAttttt                                                                                                                            >  1:2481274/1‑148 (MQ=255)
                            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                           <  1:4900720/150‑1 (MQ=255)
                             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGAAAATATtttttt                                                                                                                          >  1:1351905/1‑150 (MQ=255)
                              tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                         <  1:6083541/150‑1 (MQ=255)
                               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                        <  1:4197226/150‑1 (MQ=255)
                                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAAAGAAAATATTTTTTTTTa                                                                                                                       >  1:129545/1‑150 (MQ=255)
                                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                       <  1:2574847/150‑1 (MQ=255)
                                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTaa                                                                                                                      >  1:3860544/1‑150 (MQ=255)
                                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                      >  1:4451214/1‑150 (MQ=255)
                                  tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTtaa                                                                                                                     >  2:4675363/1‑150 (MQ=255)
                                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTATATTTAAAAATAAAATTAAATTATTTTTTTTTAAatata                                                                                                                 >  2:6292188/1‑149 (MQ=255)
                                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                               <  2:1237953/150‑1 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                              >  2:408901/1‑150 (MQ=255)
                                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                              >  2:3585753/1‑150 (MQ=255)
                                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATGAAAATATTTTTTTTTTAATATagagt                                                                                                             >  1:5852851/1‑149 (MQ=255)
                                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                          <  1:5445000/150‑1 (MQ=255)
                                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                          <  1:5522888/150‑1 (MQ=255)
                                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATAGAGCTaaag                                                                                                        >  1:5427504/1‑148 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTAAAAATATAAATAAAATATTTGTTTTTAAATATAGAGTTAACGAt                                                                                                      >  1:2065311/1‑150 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATATAGATAAAGAt                                                                                                      >  1:1217327/1‑150 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                      <  2:788068/150‑1 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAACGAt                                                                                                      >  1:6410603/1‑150 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTAAATATAGAGATAAAGAt                                                                                                      >  1:5745850/1‑150 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAATAATGAAAATAATTTTTTTTAAATAAAGAGATAAAGAt                                                                                                      >  1:3142156/1‑150 (MQ=255)
                                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTTAATATAGAGGTAACGAt                                                                                                      >  1:4731813/1‑150 (MQ=255)
                                                 gAAAATAGAATGCCGGAACCGAATAACCGCCCCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAAAAAATAAAAAAATTTTTTTAAATATAGAGaaaaatat                                                                                                      >  2:2998343/1‑146 (MQ=255)
                                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTTTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                     <  1:6774367/150‑1 (MQ=255)
                                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                     <  2:1008454/150‑1 (MQ=255)
                                                      ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAAAATAAAGAAAATATTTGTTTTTTAATATAGAGATAACGATCACaa                                                                                                 >  1:1764723/1‑150 (MQ=255)
                                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGTAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                              <  2:2797913/150‑1 (MQ=255)
                                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGATTGTTTTCGATTTCAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                              <  1:891556/150‑1 (MQ=255)
                                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCGTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCTATATCAAAATAAAATGAAAAAATTAGTTTTTAAATAAATAGATAACCATCACaaaaa                                                                                              >  1:6756254/1‑150 (MQ=255)
                                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                             <  1:1708152/150‑1 (MQ=255)
                                                          aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                             <  2:1591421/150‑1 (MQ=255)
                                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATCAAAAAAAAGACa                                                                                         >  2:3984108/1‑150 (MQ=255)
                                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACa                                                                                         >  1:6113823/1‑150 (MQ=255)
                                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTTAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACaa                                                                                        >  1:4400930/1‑150 (MQ=255)
                                                                    cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGa                                                                                   <  2:3268428/150‑1 (MQ=255)
                                                                     gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                  >  2:5091447/1‑150 (MQ=255)
                                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                 >  2:3428593/1‑150 (MQ=255)
                                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATTAAATTATTTGTTTTTAAAAATAGAGATAACGATCACAAAAAAGACAATATGAAAATTATTc                                                                          >  2:3731470/1‑150 (MQ=255)
                                                                             gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAAAATGTAAATTATTc                                                                          >  2:1826677/1‑150 (MQ=255)
                                                                               cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGGTTTCAAAAAATAAATAAAATATTTTTTTTTAAATATACAGATAAACATAACAAAAAAAACAAAATGTAAATTATTCgg                                                                        >  1:2840876/1‑149 (MQ=255)
                                                                               cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCga                                                                        >  1:5015196/1‑150 (MQ=255)
                                                                                gcgcTGTGCGTTGTTCAGCATGTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCgag                                                                       <  2:5721158/150‑1 (MQ=255)
                                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                               >  2:3810158/1‑150 (MQ=255)
                                                                                        cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTCTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                               <  2:2231285/150‑1 (MQ=255)
                                                                                            gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATAACAAAAAAGAAAATATGAAAATTATTCGAGGAGTTAAAGGCa                                                           >  2:3560938/1‑150 (MQ=255)
                                                                                              accgcttCTGGTTTGTCTATACCACTTACTTTTTCATATCGTGTGGTTTTTTTTCTTTTACAAAATATAATTAATTTTTTTTTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                         <  1:3731470/144‑1 (MQ=255)
                                                                                                  aaTCTGCTTTTTCGATACCACTTAATTTTACATATCGTGTCTTTTGTTTTCTTTTTAAAAATATATTGAATTTATTTTTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                     <  1:3426791/149‑1 (MQ=255)
                                                                                                   aTCTGCTTTGTCTAAACCACCTAAATTTACATCACCTGTAGTTTTTTTTCTATTAAAAAATATAATTAATTTATTTTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  1:3984108/150‑1 (MQ=255)
                                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                   <  2:1470924/150‑1 (MQ=255)
                                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGATATAACGATCACAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACg                                                   >  2:3219365/1‑150 (MQ=255)
                                                                                                     cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                  <  2:2555460/150‑1 (MQ=255)
                                                                                                         tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTCAAAATAAAAAGAAAGTATTTGTTTTTAAATATAGTGATAAAGATCAAAAAAACGACAATATGTAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTc                                              >  2:1955357/1‑150 (MQ=255)
                                                                                                             tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                          <  2:6610278/150‑1 (MQ=255)
                                                                                                              cGATACCACGTACATTTTCATATCCTTTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                         <  2:5151434/150‑1 (MQ=255)
                                                                                                                   ccACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAAGAAAATATTTGTTTTTAAATATAGAGATAACGATCACAAAAACCACAATATGAAAAATATTCGCGGAGTGTAAGGCAAAAAAACGGCCACCCCAGAgggc                                    >  2:3176457/1‑149 (MQ=255)
                                                                                                                     aCGTACATTTTCTTATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                  <  2:1381269/150‑1 (MQ=255)
                                                                                                                     aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                  <  2:523296/150‑1 (MQ=255)
                                                                                                                     aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                  >  2:6768184/1‑150 (MQ=255)
                                                                                                                       taaCATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                <  2:6390094/148‑1 (MQ=255)
                                                                                                                       gTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATAGAGATAAAGATCCCAAAAATGACAATATGAAAATTATTAGCGGAGTGTAAGGCCAAAAAACGGCCTCCCGCTAGGGAAGcc                                >  2:1816711/1‑150 (MQ=255)
                                                                                                                        tACATTTTCCTATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCg                               <  2:2481274/150‑1 (MQ=255)
                                                                                                                        tACATTTTCATAGCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                               <  2:5305706/150‑1 (MQ=255)
                                                                                                                        tACATTTTAATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCg                               <  1:4295519/150‑1 (MQ=255)
                                                                                                                        tACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                               <  2:2167924/150‑1 (MQ=255)
                                                                                                                         aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATCGCGATAACGATCACAAAAAAGACAATATGAAAATGATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGGAGCCGt                              >  2:3493331/1‑150 (MQ=255)
                                                                                                                         aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                              <  2:4451214/150‑1 (MQ=255)
                                                                                                                          cATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                             <  2:619738/150‑1 (MQ=255)
                                                                                                                          cATTTACATATCCTTTCGTTTGTTTTCGATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                             <  2:6726575/150‑1 (MQ=255)
                                                                                                                          aaTTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                             <  1:6620710/149‑1 (MQ=255)
                                                                                                                           aTTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                            <  2:5690343/150‑1 (MQ=255)
                                                                                                                            ttttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAGTTATTCGGGGAGTGTAAGGCAAAAAATCGGCCTCCCGCGAGGGAAGCCGTAGc                           >  2:4335640/1‑150 (MQ=255)
                                                                                                                                 atatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                      <  2:4882739/150‑1 (MQ=255)
                                                                                                                                     ccTGTCTTTTTTTTTCTATTAAAAAATATTCTCAAATTATTTGTTTTTAAATTTCTATATACCGATCAAAAAAACGAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                  <  1:6254387/150‑1 (MQ=255)
                                                                                                                                       tGTCTTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                <  2:1262057/150‑1 (MQ=255)
                                                                                                                                         tCGTTTGTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt              <  2:129545/150‑1 (MQ=255)
                                                                                                                                           gtttgtttTCGATTTCAAAATATACTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACACACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt            <  2:401055/150‑1 (MQ=255)
                                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt            >  1:154907/1‑150 (MQ=255)
                                                                                                                                           gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt            <  2:4815929/150‑1 (MQ=255)
                                                                                                                                              tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa         <  2:931512/150‑1 (MQ=255)
                                                                                                                                                   ttgATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg    >  1:3365901/3‑150 (MQ=255)
                                                                                                                                                   tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg    >  2:6542150/1‑150 (MQ=255)
                                                                                                                                                   tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTGTTATGCCg    >  2:5054606/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGA  >  NC_000913/2177080‑2177378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: