Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,187,314 C→G 5.8% intergenic (+16/+66) rcnB → / ← yehA periplasmic modulator of Ni and Co efflux/putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,187,3140CG 5.8% 1286.1 / 57.2 361intergenic (+16/+66)rcnB/yehAperiplasmic modulator of Ni and Co efflux/putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (151/189);  new base G (14/7);  total (165/196)
Fisher's exact test for biased strand distribution p-value = 6.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

Only 100 of 375 total aligned reads displayed.

GGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACTGG  >  NC_000913/2187165‑2187462
                                                                                                                                                     |                                                                                                                                                    
ggCAGTTGCGTAACCTGCCCGCGCCTGTTGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCtcatc                                                                                                                                                      <  1:6241471/150‑1 (MQ=255)
ggCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCtcatc                                                                                                                                                      <  2:3123539/150‑1 (MQ=255)
ggCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCtcatc                                                                                                                                                      <  1:1665902/150‑1 (MQ=255)
    gTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGa                                                                                                                                                  >  2:1808990/1‑150 (MQ=255)
     ttGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAg                                                                                                                                                 >  1:6562384/1‑150 (MQ=255)
      tGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgg                                                                                                                                                <  1:5079465/150‑1 (MQ=255)
      ggCGTCACCGGCCCGCGCCGGATGCCGGGACGCACGGGACCTATCGGGGGGGCGCGTCCGTGTTGATCAGCGCCCCCGACGGTAAAATCATTAAAGCCTCCGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgg                                                                                                                                                <  2:3521228/149‑1 (MQ=255)
       gCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAACTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAggg                                                                                                                                               <  2:5338910/150‑1 (MQ=255)
       gCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAggg                                                                                                                                               <  1:213693/150‑1 (MQ=255)
        cGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAgggg                                                                                                                                              <  1:5166792/150‑1 (MQ=255)
         ttAACCGGCCCGCGCCTGATGCCGGGACGCACTGGACCTATCTGGGTGGCGCGTACGTGTTGATCAGCGAATCCGACGGTAAACTAATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAATGCCCCCTCATGATGAggggg                                                                                                                                             <  2:4372596/149‑1 (MQ=255)
         gTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGCCCGATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTCCGACGGTGAGATTTTTTATCATCGCGAAAAAAAGCCCCCACATCCTGAggggg                                                                                                                                             <  2:3404239/150‑1 (MQ=255)
          tAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATGATGAgggggc                                                                                                                                            >  1:2812689/1‑149 (MQ=255)
          tAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGa                                                                                                                                            <  1:97381/150‑1 (MQ=255)
            aCCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGaaa                                                                                                                                          >  1:4129204/1‑150 (MQ=255)
              cTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATg                                                                                                                                        >  1:2020928/1‑150 (MQ=255)
              cTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATg                                                                                                                                        >  1:186414/1‑150 (MQ=255)
              cTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATg                                                                                                                                        <  1:2830101/150‑1 (MQ=255)
               tGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTATGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGACATGc                                                                                                                                       >  2:4426828/1‑150 (MQ=255)
                 cccGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAg                                                                                                                                     <  2:2206865/150‑1 (MQ=255)
                   cgcgCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGac                                                                                                                                   >  2:4194666/1‑150 (MQ=255)
                     cgcCTGATGCCGGGACGCACTGGACCTATCTGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGacac                                                                                                                                 <  2:2120676/150‑1 (MQ=255)
                        cTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCtt                                                                                                                              <  2:4977031/150‑1 (MQ=255)
                         tGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTg                                                                                                                             <  1:2201044/150‑1 (MQ=255)
                               cGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAttt                                                                                                                       >  2:4651441/1‑150 (MQ=255)
                                gggACGCACTGGTCCTATCTGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTAAtttt                                                                                                                      <  2:3724896/150‑1 (MQ=255)
                                gggACGCACTGGACCTATATGGGTGGCGCGTCCGTTTTGATCAGCGACACCGTCGGTAAAATCAGTAAAGGCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtttt                                                                                                                      <  2:3910269/150‑1 (MQ=255)
                                gggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtttt                                                                                                                      <  1:3503365/150‑1 (MQ=255)
                                gggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAtttt                                                                                                                      <  1:5412529/150‑1 (MQ=255)
                                 ggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGTCGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAttttt                                                                                                                     <  1:2504528/150‑1 (MQ=255)
                                 ggACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTAttttt                                                                                                                     <  1:3218932/150‑1 (MQ=255)
                                   aCGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTtta                                                                                                                   <  1:3319253/150‑1 (MQ=255)
                                    cGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttat                                                                                                                  >  2:2635244/1‑150 (MQ=255)
                                        cTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatt                                                                                                              >  1:5878513/1‑150 (MQ=255)
                                        cTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTttattatt                                                                                                              >  1:124592/1‑150 (MQ=255)
                                           gACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGc                                                                                                           <  1:2612335/150‑1 (MQ=255)
                                           gACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGc                                                                                                           <  1:1144290/150‑1 (MQ=255)
                                             cctcgatGGGTGGCGCGTACGTGTTGAACCGCGACACCGACGGTAAAATCATCACAGCCTACGACGGTGAGATCTTTTCTCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTCGCCa                                                                                                         <  1:4547024/145‑1 (MQ=255)
                                               tataTGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTTGCCACt                                                                                                       <  1:4059896/150‑1 (MQ=255)
                                                      gTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGt                                                                                                >  1:2939125/1‑150 (MQ=255)
                                                       tGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTc                                                                                               >  2:2938589/1‑150 (MQ=255)
                                                       tGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTc                                                                                               >  1:1394512/1‑150 (MQ=255)
                                                         gcgcGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTctt                                                                                             >  1:3724870/1‑150 (MQ=255)
                                                         gcgcGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTctt                                                                                             <  1:4422783/150‑1 (MQ=255)
                                                           gcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttgc                                                                                           <  1:2685215/150‑1 (MQ=255)
                                                           gcgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttgc                                                                                           >  2:6578940/1‑150 (MQ=255)
                                                            cgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGATATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttgct                                                                                          >  1:4186679/1‑150 (MQ=255)
                                                            cgTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTcttgct                                                                                          >  2:3847199/1‑150 (MQ=255)
                                                               aCGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGt                                                                                       <  1:5756821/150‑1 (MQ=255)
                                                                cGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGtt                                                                                      <  1:6052796/150‑1 (MQ=255)
                                                                 gtgtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTGGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGtta                                                                                     >  1:2234657/1‑150 (MQ=255)
                                                                   gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatt                                                                                   >  2:3350437/1‑150 (MQ=255)
                                                                   gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatt                                                                                   >  1:1547175/1‑150 (MQ=255)
                                                                   gtTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatt                                                                                   >  1:1757163/1‑150 (MQ=255)
                                                                    ttGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatta                                                                                  <  1:5535219/150‑1 (MQ=255)
                                                                    ttGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatta                                                                                  <  2:5714215/150‑1 (MQ=255)
                                                                    ttGATCAGCCACACCGACGCTAAAATCAATAAAGCCTACGACCGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCACATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGttatta                                                                                  <  2:1277385/150‑1 (MQ=255)
                                                                       aTCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTc                                                                               <  1:241065/150‑1 (MQ=255)
                                                                        tCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCACCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTATCCACTTGCTCGTCTTGCTTGTTATTAGTCg                                                                              >  2:6278010/1‑150 (MQ=255)
                                                                            cGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTAtt                                                                          >  2:1124454/1‑150 (MQ=255)
                                                                            cGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTAtt                                                                          >  2:4036121/1‑150 (MQ=255)
                                                                                   gACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGtt                                                                   <  1:6411150/150‑1 (MQ=255)
                                                                                     cGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGa                                                                 <  1:6436354/150‑1 (MQ=255)
                                                                                      ggTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCCTCATGAGGGGGAAATGCAGACACCGTGTTATTTTTTATTATTAGCCACTTGCTCGTCGGGCTTGTTATTAGTCGTATTTCACGTTGAt                                                                >  2:359809/1‑150 (MQ=255)
                                                                                       gTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGAtt                                                               <  1:1023106/150‑1 (MQ=255)
                                                                                            aTCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGc                                                          >  1:2369467/1‑150 (MQ=255)
                                                                                               aTTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGt                                                       >  1:815256/1‑150 (MQ=255)
                                                                                                  aaaGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGc                                                    <  2:815256/150‑1 (MQ=255)
                                                                                                   aaGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGcc                                                   <  1:881911/150‑1 (MQ=255)
                                                                                                     gCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTGATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCCCGTTGATTAATGCGGTTGCCTc                                                 <  1:6727364/150‑1 (MQ=255)
                                                                                                       cTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCa                                               <  2:3774896/150‑1 (MQ=255)
                                                                                                        tACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAg                                              >  2:3214768/1‑150 (MQ=255)
                                                                                                           gacgGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTCTTATTAGCCACTTGCTCGTCTTGCTGGTTATTAGTCGTATTTCACGTTGAGTAATGCGGTTGCCTCCAGTgc                                           >  2:109201/1‑150 (MQ=255)
                                                                                                                  agaTTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGAt                                    <  2:6431698/150‑1 (MQ=255)
                                                                                                                   gaTTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCa                                               >  1:3895733/1‑138 (MQ=255)
                                                                                                                      tttttATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTaa                                <  2:6201904/150‑1 (MQ=255)
                                                                                                                             catcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgtt                         <  2:326616/150‑1 (MQ=255)
                                                                                                                               tcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgtttg                       >  1:3140921/1‑150 (MQ=255)
                                                                                                                               tcGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACtttgtttg                       >  1:4311953/1‑150 (MQ=255)
                                                                                                                                 gCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTa                     <  2:1662667/150‑1 (MQ=255)
                                                                                                                                 gCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTa                     <  1:1124275/150‑1 (MQ=255)
                                                                                                                                 gCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTa                     <  1:3434404/150‑1 (MQ=255)
                                                                                                                                  cTAAAAAAAGCCCCCTCATCATGAGTGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTAt                    >  2:5150092/1‑150 (MQ=255)
                                                                                                                                  cTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTAt                    >  2:5501909/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCa                                               <  2:2374194/122‑1 (MQ=255)
                                                                                                                                   tAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATc                   >  2:685176/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGt                 <  2:6111033/150‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGt                 >  1:1642600/1‑150 (MQ=255)
                                                                                                                                      aaaaaGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTa                <  2:1547175/150‑1 (MQ=255)
                                                                                                                                        atttcccccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGa              >  2:1710253/5‑150 (MQ=255)
                                                                                                                                        aaaGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGc                                                                                                    >  2:6633854/1‑64 (MQ=255)
                                                                                                                                           tcccccTCATGATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt           >  1:5346501/2‑150 (MQ=255)
                                                                                                                                           gCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTCTGAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt           <  2:5858128/150‑1 (MQ=255)
                                                                                                                                           gCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt           <  1:4884370/150‑1 (MQ=255)
                                                                                                                                           gCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACgt           >  1:1746402/1‑150 (MQ=255)
                                                                                                                                             ccccTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCATAGACgtag         >  2:5620554/1‑150 (MQ=255)
                                                                                                                                              cccTCATCATGAGGGGGATATGCAGACACCTTGTTATTTTTTATTATTAGCCTCTTGCTCGTCTTGCTTGTTATTAGTCGTTTTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATcgtagtcgtagt        <  1:4004737/150‑1 (MQ=255)
                                                                                                                                                 tcatcaTGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAAc     >  2:24629/1‑150 (MQ=255)
                                                                                                                                                  catcatGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACt    >  1:5936467/1‑150 (MQ=255)
                                                                                                                                                   atcatGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACTg   <  2:6072033/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCAGTTGCGTAACCTGCCCGCGCCTGATGCCGGGACGCACTGGACCTATATGGGTGGCGCGTACGTGTTGATCAGCGACACCGACGGTAAAATCATTAAAGCCTACGACGGTGAGATTTTTTATCATCGCTAAAAAAAGCCCCCTCATCATGAGGGGGAAATGCAGACACCTTGTTATTTTTTATTATTAGCCACTTGCTCGTCTTGCTTGTTATTAGTCGTATTTCACGTTGATTAATGCGGTTGCCTCCAGTGCGCCAGATTTAACTTTGTTTGTATCGTAGACGTAGTAACTGG  >  NC_000913/2187165‑2187462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: