Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,229,177 A→C 13.7% intergenic (+112/+261) yohP → / ← dusC uncharacterized protein/tRNA‑dihydrouridine synthase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,229,1770AC13.7% 409.5 / 10.9 234intergenic (+112/+261)yohP/dusCuncharacterized protein/tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base A (56/112);  new base C (21/9);  total (90/144)
Fisher's exact test for biased strand distribution p-value = 2.14e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

ACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCA  >  NC_000913/2229029‑2229324
                                                                                                                                                     |                                                                                                                                                    
aCTGGTGGTGACTTGCGTATTTAAGATGATAATTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAg                                                                                                                                                     <  1:1235928/150‑1 (MQ=255)
 cTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAgg                                                                                                                                                    <  2:6037053/150‑1 (MQ=255)
  tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCggg                                                                                                                                                   >  1:6748884/1‑150 (MQ=255)
  tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCggg                                                                                                                                                   >  1:4762321/1‑150 (MQ=255)
  tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                   <  1:5224562/150‑1 (MQ=255)
  tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggg                                                                                                                                                   <  1:2964806/150‑1 (MQ=255)
    gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCgggggggg                                                                                                                                                 >  2:1870340/1‑150 (MQ=255)
    gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggg                                                                                                                                                 >  1:1032513/1‑150 (MQ=255)
    gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATAACTGGCGCAAGGAGCGCGGAggggg                                                                                                                                                 >  1:1019837/1‑150 (MQ=255)
    gcggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAggggg                                                                                                                                                 <  1:5438654/148‑1 (MQ=255)
     tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggc                                                                                                                                                <  1:5142486/150‑1 (MQ=255)
     tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCGAGGAGCGCGCggggggg                                                                                                                                                >  1:5639095/1‑149 (MQ=255)
      ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                               <  2:5839476/150‑1 (MQ=255)
      ggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcg                                                                                                                                               <  2:3583306/150‑1 (MQ=255)
       gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                              <  2:2727434/150‑1 (MQ=255)
       gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                              <  2:1032513/150‑1 (MQ=255)
       gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcgg                                                                                                                                              <  1:1272829/150‑1 (MQ=255)
        tgACTGGATTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcggc                                                                                                                                             <  2:3749054/150‑1 (MQ=255)
         gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGCATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCgggggggggc                                                                                                                                            >  1:275602/1‑148 (MQ=255)
         gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGcc                                                                                                                                            >  1:3919615/1‑150 (MQ=255)
         gACTGGCGTATTTAAGATGATATTTTAAAATTAAGTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCTGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGGCgggggggggc                                                                                                                                            >  1:276874/1‑148 (MQ=255)
          aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGccc                                                                                                                                           >  2:6365867/1‑149 (MQ=255)
           cTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaa                                                                                                                                          <  1:1157308/150‑1 (MQ=255)
              gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATCTAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGcccaacg                                                                                                                                       >  1:3600521/1‑145 (MQ=255)
              gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCaaaac                                                                                                                                       >  1:3600200/1‑147 (MQ=255)
                 tATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATcgccg                                                                                                                                    <  2:2717997/150‑1 (MQ=255)
                  aTTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGCGGCCCCTcgccgc                                                                                                                                   >  2:4651587/1‑150 (MQ=255)
                   tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCACTccccccc                                                                                                                                  >  1:1571545/1‑150 (MQ=255)
                        gatgatATTTTAAAATTAATTAATGTCATCTGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACGGGCGCACGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGcccc                                                                                                                             <  1:2832823/150‑1 (MQ=39)
                           gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGGCCATCCCCGccccccccag                                                                                                                          >  2:3811876/1‑148 (MQ=255)
                           gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGCGCGGGGGGGGGCCAAAAGCcccccccccccc                                                                                                                          >  1:1685193/1‑148 (MQ=255)
                               ttttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGCGGCCCAAAGCCCCCCCCCCCCGCAcc                                                                                                                      >  2:3279533/1‑150 (MQ=255)
                                    aaaTTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCCAAAccccccccccccccccccccccc                                                                                                                  >  1:2578195/1‑149 (MQ=255)
                                     aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcggc                                                                                                                                             >  2:3497136/1‑121 (MQ=255)
                                     aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGggcggc                                                                                                                                             >  1:309445/1‑121 (MQ=255)
                                        taattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAACCCGCCCCCCCCAGCACCCCCCGGcgc                                                                                                             >  2:498081/1‑148 (MQ=37)
                                         aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGctct                                                                                                            <  2:948198/150‑1 (MQ=255)
                                            taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATAGAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCCAACGCCCCCGCCCCCCCCACCCCCCCGCGCTGGc                                                                                                         >  1:4139115/1‑150 (MQ=37)
                                               tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGaa                                                                                                     <  2:5879911/150‑1 (MQ=255)
                                                   atcaGGTCCGAACATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaa                                                                                                 <  2:2117012/150‑1 (MQ=255)
                                                   atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCCTGTGCATTGCGTCATATAATCACTGGCGCAAGGAGCGCGCCCGGGGCGGCCCAACGCCCCCCCCCCCAGCCCCCCCCCGCGCGGGCGcacaca                                                                                                 >  2:364047/1‑148 (MQ=255)
                                                   atcaGGCCCGAACATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCGCCGGTCATGTGCATTGCTTCTTATAATCCCTGGCGCACGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACaaa                                                                                                 <  2:1204055/150‑1 (MQ=25)
                                                    tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCCAACGCCGCCCCCCCCCGCCCCCCCCGGCGCGGGCGGAACaaa                                                                                                 >  2:2919044/1‑150 (MQ=25)
                                                      aGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGGGGGGGGGGGCCCACCCCCCCCCCCCCCCCCCCCCCCCGCGCAGGCGCACaaaaaa                                                                                              >  1:952233/1‑148 (MQ=255)
                                                        gtcataaaaTAACGATAATTTTTCAGTCTCTCTTCGTGTTGCGCTCCTGTCATGTGCTTTGCTACATATAACCCCGGGCGCAAGGTGGGCGCTGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                            <  1:6020367/145‑1 (MQ=17)
                                                        caccGAAAATAACGAGAAAAATACACACACACATCCTGTTGCGCTCCTGACATCTGCATTGCTTCAAATAATCACTCGCGCAAGGACCGCGCAGGGGGCGCCCAAACCCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                            <  2:4681803/148‑1 (MQ=18)
                                                              aaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTgc                                                                                      <  2:658840/150‑1 (MQ=255)
                                                                    cGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTg                                                                                <  2:3335973/150‑1 (MQ=255)
                                                                        aaTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAACCCGCCCCCCCCAGCAACCCCCGGCACAGGCGAAAAAAAAAGACCCAGCGCCGTGccca                                                                            >  2:396190/1‑149 (MQ=21)
                                                                         atatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCATGGAGGGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                           <  2:2322034/150‑1 (MQ=255)
                                                                         atatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                           <  2:4942773/150‑1 (MQ=255)
                                                                         atatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTAATAAAAACACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGATCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc                                                                           <  2:4146339/150‑1 (MQ=255)
                                                                           atTTCCGTCTCTCTCCGTTTGGGGCTCCTGTCTTGGGCTTGGCTACATATAACCCCGGGCGCACGGCGCGCGCGGGGGGCGCCCAAGCGCCGCCGCCCCCGCCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                         <  1:4815780/150‑1 (MQ=2)
                                                                           atTTCAGTCTCTCATCCGGTGGCGCTCCTGTCCTGTGCTTTGCTTCTTATAATCACGGGCGCACGGCGCGCGCTGGGGGCGGCCAAGCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCgg                                                                         <  1:3532500/150‑1 (MQ=18)
                                                                             ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                       <  2:3231276/150‑1 (MQ=255)
                                                                                aGTCTCTAATCCGTTTGCGCTCCTGTCATGTGATTTGCTTATTATAATCACTGGCGCACGGTGCGGGCCGGGGGGGGCCTCTCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCTAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt                                                                    <  1:5852708/150‑1 (MQ=17)
                                                                                  tctctcATCCTGTTGCGCTCCTGTAAGGTGCATTGCTTCATATAATCACTGGCGCACGGTGCGCGCTGGGGGCGGCCAACCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                  <  1:942416/150‑1 (MQ=18)
                                                                                  tctctcAGCCTGTTGCGCTCCTGTCATGTGAATTGCTTCATATACTCCCGGGCGCACGGCGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATcc                                                                  <  1:5276250/150‑1 (MQ=18)
                                                                                    tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATAGCCGCGGCGCGGTGCCCAACGCATATccca                                                                >  2:5096038/1‑149 (MQ=18)
                                                                                     ctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCCCCCCCGGGCACAGGCGACCAAAATCGCCGCGGCGCGGGGCCCGCGGCtttaaccat                                                               >  2:4328852/1‑143 (MQ=18)
                                                                                      tcATCGTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                              <  2:5230424/150‑1 (MQ=255)
                                                                                         tCCTGTTGCGCTCCTGTCATGTGCTTTGCTTCATATAATCACTGGCGCACGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                           <  2:3066877/150‑1 (MQ=255)
                                                                                         tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAACGCCCCCCCCCCCCGCACCCCCCCGCGCTGGCGCACAAAAACGCCCCCGCGCGGTGCCCCCCGCTTATCCCATACgg                                                           >  2:2785685/1‑150 (MQ=18)
                                                                                            tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCCGGGGGCGGCCAAACGCCGCCCCCCCCAGCACCCCCCGGCGCAGGCGAACAAAATAGCCCCAGCGCGGTGCCCTCGGCTTATACCATACGGCTa                                                        >  2:6032968/1‑150 (MQ=17)
                                                                                             gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCCCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc                                                       <  1:4991623/150‑1 (MQ=255)
                                                                                              ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCACGGGGCGGCCAAGCCCCCCCCCCCCCACCACCCCCCGGCGCGGGCGAACAAAAACGCCCCCGCGCCGGGCCCACCGCGTAACCCGTACCGCAAcc                                                      >  2:2229908/1‑150 (MQ=12)
                                                                                               tGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCg                                                     <  1:4511016/150‑1 (MQ=255)
                                                                                                gcgcTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCgg                                                    <  2:5460235/150‑1 (MQ=255)
                                                                                                gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGATTATCCCTTACGGCTACCgg                                                    <  2:6205117/150‑1 (MQ=255)
                                                                                                     ccTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCgg                                               <  2:136084/150‑1 (MQ=255)
                                                                                                           aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCCAAACCCCCCCCCCCCCGCACCCCCCCGCGCGGGCGCACCAAAACCCCCCCGCCCGGGGCCCCCCGCGTATCCCCAACGGCGACCCGGGCGGGcgcgc                                         >  2:4885741/1‑149 (MQ=12)
                                                                                                           aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                         <  2:4726392/150‑1 (MQ=255)
                                                                                                            tgtgCATTGCTTCATATAATCCCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                        <  1:498069/150‑1 (MQ=255)
                                                                                                            tgtgCATTGCTTCATATAATCCCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                        <  1:4805611/150‑1 (MQ=255)
                                                                                                            tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                        <  1:3649820/150‑1 (MQ=255)
                                                                                                             gtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCCGCACAGGCGAACAAAAACGCCCCGGCGCGGGGCCCGCGGCCGATACCATACCGCGACCCGGGCGGGCGCGCgg                                       >  2:5587730/1‑150 (MQ=9)
                                                                                                              tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCgccgccgcc                                                                                                                               >  2:6517718/1‑62 (MQ=255)
                                                                                                               gCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTa                                     <  1:187315/150‑1 (MQ=255)
                                                                                                                cATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                    <  2:6396923/150‑1 (MQ=255)
                                                                                                                   cgCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                 <  2:2565101/149‑1 (MQ=255)
                                                                                                                    gCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATc                                <  2:597087/150‑1 (MQ=255)
                                                                                                                       tCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                             <  1:5662223/150‑1 (MQ=39)
                                                                                                                       tCATATAATCACGGGCGCAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt                             <  2:6230363/150‑1 (MQ=255)
                                                                                                                             aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGCTCGGGCGAACAAAATCGCCGCTGCGCGGTGCCCGCGGCGTATACCGTACGGCTACCCGGGAGGGCGCGAGGTAACAAACCCGTaaaa                       >  2:524924/1‑150 (MQ=11)
                                                                                                                             aaTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCAATGGCGAACAAAATAGCCCCAGCGCGGTGGCCACGGCGTATCCCATAACGCAACCGGGTCGGGCGCGCGGTAACAAACCAGTaaaa                       >  2:4531870/1‑150 (MQ=2)
                                                                                                                              aTCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAc                      <  1:2474346/150‑1 (MQ=33)
                                                                                                                                 ccTGGCGCACGGAGCGCGCCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                   <  1:2900899/149‑1 (MQ=17)
                                                                                                                                 aCTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgcg                   <  1:4329205/150‑1 (MQ=32)
                                                                                                                                   tGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGcc                 <  2:4456689/150‑1 (MQ=32)
                                                                                                                                      cgcAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTc              <  1:3446857/150‑1 (MQ=17)
                                                                                                                                        cAAGGAGCGCGCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg            <  1:4658517/150‑1 (MQ=17)
                                                                                                                                           ggAGCGCGCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGccc         <  1:612379/150‑1 (MQ=17)
                                                                                                                                             aGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcac       <  2:4762321/150‑1 (MQ=32)
                                                                                                                                               cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt     <  1:2145975/150‑1 (MQ=32)
                                                                                                                                               cgcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATACCTTACGGCTACCGGGTCGGGCGCGCGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAt     >  2:4944281/1‑150 (MQ=12)
                                                                                                                                                gcgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc    >  2:6718372/1‑150 (MQ=32)
                                                                                                                                                 cgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAtgg   <  2:759879/150‑3 (MQ=17)
                                                                                                                                                 cgcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc   <  2:910514/150‑1 (MQ=32)
                                                                                                                                                   gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa  <  2:565012/150‑1 (MQ=32)
                                                                                                                                                   gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCa  <  2:4568041/150‑1 (MQ=32)
                                                                                                                                                     |                                                                                                                                                    
ACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCA  >  NC_000913/2229029‑2229324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: