Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 151,688 C→G 9.0% V182L (GTA→CTA)  yadL ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913151,6880CG 9.0% 1146.4 / 12.5 356V182L (GTA→CTA) yadLputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (193/124);  new base G (2/37);  total (195/161)
Fisher's exact test for biased strand distribution p-value = 3.95e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

AAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGA  >  NC_000913/151539‑151837
                                                                                                                                                     |                                                                                                                                                      
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                        <  2:1160728/150‑1 (MQ=255)
aaaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTAc                                                                                                                                                        >  1:1696183/1‑150 (MQ=255)
 aaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACg                                                                                                                                                       >  2:848011/1‑150 (MQ=255)
 aaaCAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACg                                                                                                                                                       >  2:736942/1‑150 (MQ=255)
            acagaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttt                                                                                                                                            >  1:1148198/1‑150 (MQ=255)
              agaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTAttttttt                                                                                                                                          >  2:4527684/1‑150 (MQ=255)
               gaaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTc                                                                                                                                        <  2:2078310/150‑1 (MQ=255)
                aaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATATTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTTCAGAAAACTTACCAGCATTGAAATTGTTTACGGTTTTATTTTTTTcc                                                                                                                                       <  2:4252618/150‑1 (MQ=255)
                aaacagaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTcc                                                                                                                                       >  1:5521492/1‑150 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   <  2:5369677/150‑1 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   <  1:3361102/150‑1 (MQ=255)
                    agaaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa                                                                                                                                   >  2:1509722/1‑150 (MQ=255)
                      aaTTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGAcc                                                                                                                                 <  1:5469467/150‑1 (MQ=255)
                       attattCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCa                                                                                                                                >  2:2993217/1‑150 (MQ=255)
                       attattCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCa                                                                                                                                <  2:1732857/150‑1 (MQ=255)
                          attCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGc                                                                                                                             <  2:5400514/150‑1 (MQ=255)
                            tctcTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGt                                                                                                                           <  2:5827304/150‑1 (MQ=255)
                             ctctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTg                                                                                                                          <  1:2454706/150‑1 (MQ=255)
                               ctTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCa                                                                                                                        <  1:5937107/150‑1 (MQ=255)
                                ttCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCAc                                                                                                                       >  2:1959796/1‑150 (MQ=255)
                                   atcaGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa                                                                                                                    >  2:6308303/1‑150 (MQ=255)
                                   atcaGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa                                                                                                                    >  1:4307430/1‑150 (MQ=255)
                                    tcaGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCAt                                                                                                                   <  1:2994442/150‑1 (MQ=255)
                                     caGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATc                                                                                                                  <  1:468251/150‑1 (MQ=255)
                                       gCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCgg                                                                                                                >  2:4815628/1‑150 (MQ=255)
                                       gCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCgg                                                                                                                >  2:5662335/1‑150 (MQ=255)
                                        cTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGa                                                                                                               <  2:3537090/150‑1 (MQ=255)
                                              ttGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                         <  1:2685068/150‑1 (MQ=255)
                                               tGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                        <  1:6714361/150‑1 (MQ=255)
                                                gAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAAc                                                                                                       <  1:1509722/150‑1 (MQ=255)
                                                 aGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACg                                                                                                      >  1:6599446/1‑150 (MQ=255)
                                                         gCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAtt                                                                                              >  1:6582008/1‑150 (MQ=255)
                                                           atatatTGTTCCTTAATTTTTATTTCTGTTTGTTATTGTTTACTTTAATTGTGGCTGGTGCATAAAACTTACCCGCTGTGACATTGTTTAGGTTTTTATTTTTTTCCACGACCCGGCGTGCACTCATCGTATAATAAACGACCGCATTgg                                                                                            <  2:3612494/150‑1 (MQ=255)
                                                           atatatTGTTCCTGAATCTTTAAGTGTTTTTGTTATTGGTTAGTTTAATTGTGGCTGGTGCATAAAACTTACCCGCTGTGACTTTTTTTAGGTTTTTTTTTTTTTCCACGACCAGGGGTGCACTCCTGGGATAATAAACGACCGCATTgg                                                                                            <  2:146950/150‑1 (MQ=255)
                                                           atatatAGTTCCTGAATATGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACGTTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTgg                                                                                            <  2:1684768/150‑1 (MQ=255)
                                                              tataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGt                                                                                                                           <  2:659520/116‑1 (MQ=255)
                                                                   ttCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTg                                                                                    >  2:379197/1‑150 (MQ=255)
                                                                      cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgag                                                                                 >  2:263392/1‑150 (MQ=255)
                                                                       tgtaTCTGTAAGTCTGTTAGTTTTTGGTTTCTTTAATTGTGGGTGTTGCAGAAAACTTACCCGCAGTGACATTGTTTAGGTTTTTATTTTTTTCCACGACCCGGGGTGAAATCATCGTATAATAAACGACCGCATTGGCTGCAGTGgaga                                                                                <  2:4770646/147‑1 (MQ=255)
                                                                       tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                         <  2:2577216/125‑1 (MQ=255)
                                                                       tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGAtaataa                                                                                                         >  1:2577216/1‑125 (MQ=255)
                                                                       tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgaga                                                                                >  1:2066892/1‑150 (MQ=255)
                                                                        gAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAt                                                                               >  2:1060966/1‑150 (MQ=255)
                                                                             tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                          >  2:3242359/1‑150 (MQ=255)
                                                                             tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                          >  1:2206486/1‑150 (MQ=255)
                                                                             tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                          >  2:6020370/1‑150 (MQ=255)
                                                                              gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTAc                                                                         >  1:4544170/1‑150 (MQ=255)
                                                                               tAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTAcc                                                                        >  1:3102404/1‑150 (MQ=255)
                                                                                 aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACctc                                                                      >  2:4927800/1‑150 (MQ=255)
                                                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                    >  1:5095/1‑150 (MQ=255)
                                                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                    >  2:4474344/1‑150 (MQ=255)
                                                                                   tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCtt                                                                    >  1:6052050/1‑150 (MQ=255)
                                                                                     tGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTg                                                                                                                          >  2:2060740/1‑94 (MQ=255)
                                                                                     tGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgt                                                                  >  1:3836602/1‑150 (MQ=255)
                                                                                      gttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTtgtg                                                                 >  2:2749883/1‑150 (MQ=255)
                                                                                        tagttaTTGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCTGTGACATTGTTTAGGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGAt                                                               <  2:5611690/150‑1 (MQ=255)
                                                                                         agttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATg                                                              >  1:3511286/1‑150 (MQ=255)
                                                                                            tatgGGTTACTTTATTTGGGGTTGGTGCAAAAAACTCACCCGCTTTTACTTTTTTTCGTTTTTTTTTTTTTCCCCCCCCCGGGCGTGCACTCTTCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTg                                                           <  1:1270137/150‑1 (MQ=255)
                                                                                              tctgTTACTTTATTTGTGGCTTGTCAACAAAACTAACCCGCTGTTAAATTTTTTCGTTTTTTTTTTTTTCCCACCACCCGGCGTGCACTCATGGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgt                                                         <  1:839460/147‑1 (MQ=255)
                                                                                                 gTTACTTAAATTGTGGCTGGTCAATAAAACTAACCCGCTGTCACTTTGTTTAGGTTTTTTTTTTTTACCACGACCAGGGGGCCACTCATGTTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGtt                                                      <  1:5333877/150‑1 (MQ=255)
                                                                                                 gTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGtt                                                      >  2:1227052/1‑150 (MQ=255)
                                                                                                  tatctttAATTGTGGCTGGTGAATAAATCTTCCCCGCCTTGAAATTGTTTCGGTTTTTATTTTTTCCCACGACCTGGCGTGCACTCACCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTa                                                     <  1:4051676/144‑1 (MQ=255)
                                                                                                   tACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                    >  1:4518064/1‑102 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgt                                                         >  1:4805218/1‑142 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                 >  2:5549968/1‑150 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                 >  2:348841/1‑150 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                 >  2:3784591/1‑150 (MQ=255)
                                                                                                      tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                 >  2:5358458/1‑150 (MQ=255)
                                                                                                          aaTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTa                                             >  1:2300923/1‑150 (MQ=255)
                                                                                                           ttgtgGTTGGTGATTAAATCTTACCCGCATTGACTTTTTTTCGGTTTTTTTTTTTTTCCACTACCTGGCTTGCACTCTTGTTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTaa                                            <  1:6219201/149‑1 (MQ=255)
                                                                                                             gtgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAt                                          >  1:4828589/1‑150 (MQ=255)
                                                                                                              tgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAAtt                                         >  2:6773584/1‑150 (MQ=255)
                                                                                                                gCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATtgt                                       >  1:1061949/1‑150 (MQ=255)
                                                                                                                   ggTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGtt                                    >  1:5777532/1‑150 (MQ=255)
                                                                                                                    gTGCATAAAACTTACCCGCTGTTACATTGTTTAGGTTTTTTTTTTTTTCCACGACCCGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTg                                   <  2:1316097/150‑1 (MQ=255)
                                                                                                                      gCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCCCGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCa                                 <  2:6696449/150‑1 (MQ=255)
                                                                                                                      gCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCa                                 >  1:5609160/1‑150 (MQ=255)
                                                                                                                         gAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTc                              >  1:3316927/1‑150 (MQ=255)
                                                                                                                          aaaaCTTCCCCGCTGTGAATTTTTTTAGGTTTTTTTTTTTTACCCCGACCCGGCGTGCACTCAGCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                             <  1:3613266/150‑1 (MQ=255)
                                                                                                                          aaaaCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                             >  2:507329/1‑150 (MQ=255)
                                                                                                                          aaaaCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTGCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                             <  2:1635982/150‑1 (MQ=255)
                                                                                                                          aaaaATTCCCCGCTGTGACTTTGTTTAGGTTTTTATTTTTTTCCACGACCCGGCGTGCACTCATGGGATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                             <  2:4086933/150‑1 (MQ=255)
                                                                                                                               ttACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGtt                        >  2:3320921/1‑150 (MQ=255)
                                                                                                                               ttACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGtt                        >  1:2794022/1‑150 (MQ=255)
                                                                                                                                   ccGCTGTGACCTGGTTTCGGGTTTTATTTTTTTCCCCGCCCCGGCGTGCCCGCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    <  1:4432942/150‑1 (MQ=255)
                                                                                                                                   ccGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                    >  2:5000185/1‑150 (MQ=255)
                                                                                                                                     gCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                  >  2:2948705/1‑150 (MQ=255)
                                                                                                                                     gCAGTCACTTTGTTTAGGGTTTTATTTTTTCCCCCGCCCAGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                  <  1:6238088/150‑1 (MQ=255)
                                                                                                                                           actttGTTTCCGGTTTTATTTTTTTCCACGACCCGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa            <  1:981095/147‑1 (MQ=255)
                                                                                                                                           aCATTGTTTAGGGTTTTATTTTTTTCCCCGACCAGGCGTGCACACATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACATAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa            <  1:895940/150‑1 (MQ=255)
                                                                                                                                              ttGTTTCGGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTg         <  1:1902532/150‑1 (MQ=255)
                                                                                                                                              ttGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTg         <  1:3902760/150‑1 (MQ=255)
                                                                                                                                               tGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAg                                                >  1:6521640/1‑110 (MQ=255)
                                                                                                                                                gTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCt       >  2:1800775/1‑150 (MQ=255)
                                                                                                                                                 tttCCGGTTTTATTTTTTCCCCCGCCCCGGCGTGCCCTCAGCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTc      <  1:5298373/150‑1 (MQ=255)
                                                                                                                                                  ttACGGTTTTATTTTTTTCCACGACCAGGCGGGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTg                                                                             <  2:2257535/78‑1 (MQ=255)
                                                                                                                                                   tACGTTTTTTTTTTTTCCCCCGACCAGGCGTGCACTCAGCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCa    <  1:1418310/150‑1 (MQ=255)
                                                                                                                                                   tACGTTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCa    <  1:701935/150‑1 (MQ=255)
                                                                                                                                                     cGGTTTTATTTTTTTCCACGACCTGGCGTGCACTCATCGTATAATACACGACCGCATTGGCCGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGa  <  2:654839/150‑1 (MQ=255)
                                                                                                                                                     cGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGa  >  1:5242227/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGA  >  NC_000913/151539‑151837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: