Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,427 C→A 10.2% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4270CA10.2% 789.7 / 18.0 262Q433K (CAA→AAA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (80/148);  new base A (32/2);  total (112/150)
Fisher's exact test for biased strand distribution p-value = 2.01e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

TTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470278‑2470576
                                                                                                                                                     |                                                                                                                                                        
   tagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTaaaa                                                                                                                                                       >  1:2306579/1‑150 (MQ=255)
   tagtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaaa                                                                                                                                                       >  2:5368712/1‑150 (MQ=255)
    agtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTaaaat                                                                                                                                                      >  2:1011422/1‑149 (MQ=255)
     gtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGa                                                                                                                                                     >  2:5627540/1‑150 (MQ=255)
      taTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                    <  1:4768056/150‑1 (MQ=255)
           ctTTTTGGAATATCTCAATTTTCTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                               <  1:3791809/150‑1 (MQ=255)
           ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                               <  1:5364255/150‑1 (MQ=255)
           attttttGAATATCTCAATTTTATGTGTCATTTTTCGGGGTAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGAGCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCTAAGAAATCaa                                                                                                                                               <  2:3610119/149‑1 (MQ=255)
                  gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                    >  1:6592794/1‑138 (MQ=255)
                  gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGaa                                                                                                                                                    <  2:6592794/138‑1 (MQ=255)
                      atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                    <  1:3661110/150‑1 (MQ=255)
                       tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                   <  2:2113761/150‑1 (MQ=255)
                        ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAATTCAAAAAAAAAAAAACAAAAACGaag                                                                                                                                  >  1:5226630/1‑148 (MQ=255)
                         tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCaaa                                                                                                                                                       <  1:1646829/128‑1 (MQ=255)
                         tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAATAAAATAGCGATGt                                                                                                                                 >  2:1137058/1‑150 (MQ=255)
                         tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAAACAAAATCGATGt                                                                                                                                 >  1:1602448/1‑150 (MQ=255)
                          cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                                                                                                <  1:4452365/150‑1 (MQ=255)
                             ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                             <  2:1684481/150‑1 (MQ=255)
                             ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACATTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAATAAAACAAGACAAAAGCGAATTaaaa                                                                                                                             >  2:969964/1‑149 (MQ=255)
                             gtttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAAATAAAAAAAAAAAAAAAAATATCGATTTAAAg                                                                                                                             >  1:1276768/2‑150 (MQ=255)
                               ttATGTGTCATTTTTCGGGGAAGGATGTAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                           <  2:1503032/150‑1 (MQ=255)
                               ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                           <  2:2725673/150‑1 (MQ=255)
                                 aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                                                                                                         <  2:1932878/150‑1 (MQ=255)
                                    tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAATAAAAAAAGAAAAAATCGATGTTAATCACAAAg                                                                                                                      >  1:5644875/1‑150 (MQ=255)
                                      tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATGTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGAAAATATCGATTTAAATCaaaaaatt                                                                                                                    >  1:2192532/1‑143 (MQ=255)
                                         tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                 <  1:6564580/150‑1 (MQ=255)
                                          ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAGAGCACTAAGTTTaaa                                                                                                                <  2:6729071/150‑1 (MQ=255)
                                          ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                <  2:2011431/150‑1 (MQ=255)
                                               ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAACGATGTAAAGACCGAAGTTTAAATTtcg                                                                                                           >  1:1488805/1‑150 (MQ=255)
                                                gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                          <  1:672185/150‑1 (MQ=255)
                                                gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                          <  1:5165350/150‑1 (MQ=255)
                                                    aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                                                                                                      <  1:1137058/150‑1 (MQ=255)
                                                      gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATTTAAATCACTAAGTTTAAATTTCGCGCCAAt                                                                                                    >  2:375032/1‑150 (MQ=255)
                                                        tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAATACAATAGCGATGTAAAGCAATAAGTTTAAATTTCGCGCCAAACa                                                                                                  >  2:3269655/1‑150 (MQ=255)
                                                         ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                                 <  2:530135/150‑1 (MQ=255)
                                                          taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAATTCAAATAAAACAATACAAAAACGAAGTAAATCACGAAGTTTAAAATTCGCGCCAAACATg                                                                                                >  1:967651/1‑150 (MQ=255)
                                                           agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATCAAGGCAAAATCGATGTAAAGCCCTAGGTGTTTATTGGGCGGCAAGCCTgg                                                                                               >  2:5806741/1‑150 (MQ=255)
                                                           agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAATAAATAAAAGAAAACAAAACAATATCGATTTGAAGCACAAAATTTTAATTGCGCGCCacacaggg                                                                                               >  1:6753948/1‑143 (MQ=255)
                                                            gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTAAAATAAATAAAATAAAAAAAGACAATAGCGATTGAAATCACTAAGTTTAAATTGAGCGCCCAtaaaggg                                                                                              >  2:2519168/1‑144 (MQ=255)
                                                            gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                              <  1:879670/150‑1 (MQ=255)
                                                               aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAATCGATGTAAAGCACTAATTTTAAATTTCGCGCCAAACAATgcgcg                                                                                           >  2:4860678/1‑150 (MQ=255)
                                                                    agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                      <  2:4782429/150‑1 (MQ=255)
                                                                      caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAATAAATAAAAAATAGATTTAAAGCACAAAATTTAAATTGAGCGCCAAAAAAGGCGCGCACaaac                                                                                    >  2:2367761/1‑148 (MQ=255)
                                                                      caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAATAAAAAAAAAAAAAAGAGATGTAAAGCACAAAGATTAAAATTCGAGCCAATCAAGGCGCGCACaacc                                                                                    >  2:1307408/1‑148 (MQ=255)
                                                                      caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTTTTAAAAAAATAAAAAAAAAAAAGACAAAAACGCTGTAAATCACTAAATTTAAAATTCGCGCCCAACAATGCGCGCCCaaaa                                                                                    >  1:6258954/1‑148 (MQ=255)
                                                                       aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaatttaa                                                                                   <  2:230715/150‑7 (MQ=255)
                                                                       aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                                   >  2:1046663/1‑150 (MQ=255)
                                                                       aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAAAAAAAAAAAAAAAAACCATGTAAATCACAAAGTTTAAATTTCGCGCCCATAAGGGCGCGCACaaaca                                                                                   >  1:3801374/1‑147 (MQ=255)
                                                                        agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAAAAAATAAAAAAGAGATTTAAAGCACAAAATTTAAATTTAGCGCCACACAAGGCGCGCACaaacaa                                                                                  >  1:1010276/1‑146 (MQ=255)
                                                                           atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataa                                                                               <  2:4488549/150‑1 (MQ=255)
                                                                            tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAGAAAAAATCGATGTAAAGCACAAAGTTTAAAATTCGCGCCAATAAATGCCCGCAAAACCAataaa                                                                              >  2:5932191/1‑149 (MQ=255)
                                                                             ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                             <  2:1217388/150‑1 (MQ=255)
                                                                             ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                             <  2:1217652/150‑1 (MQ=255)
                                                                               atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAAACATGGCGCGCACAAGCAataataac                                                                           >  2:6212706/1‑148 (MQ=255)
                                                                                tttGGAAGTTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCa                                                                          <  2:5702392/150‑1 (MQ=255)
                                                                                         tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAATCACTAAGTTTAAATTGAGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                                 >  2:4275491/1‑150 (MQ=255)
                                                                                           tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACAAAGTTTAAATTGAGCGCCAATCATGGCGCGCAAAAGCTATAATAACAACCTAATTt‑‑‑ct                                                            >  2:6089812/1‑150 (MQ=255)
                                                                                            ttttCGTTCTACCTTTGATTATACATAACAGTCTTTTTTTTAATTTAAAAAATAATTAAAATAAATCAAGACAATAGCTATGTAAAGCAATATTTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTt‑‑‑ctc                                                           <  1:5759287/150‑1 (MQ=255)
                                                                                             tttCGTTCTACCTTTGCTTATACATAACCGTCTTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑ctcc                                                          <  1:1383551/150‑1 (MQ=255)
                                                                                                  ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑ctcctcctc                                                     >  2:3884314/1‑150 (MQ=255)
                                                                                                    cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCtt                                                   <  2:3418041/150‑1 (MQ=255)
                                                                                                        cTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATAAAAAATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAACAACCAAATTT‑‑‑ATCCTCCGCTTagat                                               >  2:4366455/1‑149 (MQ=255)
                                                                                                         tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGt                                              <  1:5688199/150‑1 (MQ=255)
                                                                                                         tttGATTAGAAATAACAGTCGTTTTTTTAAAGTATACAATAATTCAAAGAAATCATGACCATAGAGATGTAAAGCACTAAGTTTCAATTGATCGCCACTCATGTCGCGAACATGCTATAATACCACCCTAATTT‑‑‑CTCCTCCTCTTAGAGt                                              <  2:4662867/150‑1 (MQ=255)
                                                                                                           tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGa                                            <  1:3107980/150‑1 (MQ=255)
                                                                                                              ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAATACAAAAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAAACATGGCGCGCACAAGCTATAATACCAACCAAAATT‑‑‑CTACTCCTCTTAGAGTGACta                                         >  2:1040985/1‑150 (MQ=255)
                                                                                                               tataCATAACATTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACtat                                        <  2:948382/150‑1 (MQ=255)
                                                                                                                    aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATAtctc                                   <  1:3439834/150‑1 (MQ=255)
                                                                                                                      aaCAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCt                                 <  1:6364487/150‑1 (MQ=255)
                                                                                                                          gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATa                             <  1:1046663/150‑1 (MQ=255)
                                                                                                                           tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTAAATCTCCTGATAg                            >  2:2474899/1‑150 (MQ=255)
                                                                                                                               ttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAtt                        <  1:2554608/150‑1 (MQ=255)
                                                                                                                                tttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGAACAAGCTATAATACCAAACTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATCTCTCCTGATAGAATTg                       >  2:6589223/1‑150 (MQ=255)
                                                                                                                                 ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACACGCTATCATACCAACCTAAATT‑‑‑CTCCTCCGCTTAGAGTGACTATATCTCCTGATAGAATTGc                      >  2:5319067/1‑150 (MQ=255)
                                                                                                                                 ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                      <  2:3134267/150‑1 (MQ=255)
                                                                                                                                  tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  2:1241125/150‑1 (MQ=255)
                                                                                                                                  tttAATTTATAAAATAATACAAAGAAATCAAGACAATAGCTATTTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                     <  1:2379595/150‑1 (MQ=255)
                                                                                                                                   ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                    <  2:1316513/150‑1 (MQ=255)
                                                                                                                                   ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                    <  2:551567/150‑1 (MQ=255)
                                                                                                                                    tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                   >  1:2378123/1‑150 (MQ=255)
                                                                                                                                         tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa              >  1:3320291/1‑150 (MQ=255)
                                                                                                                                         tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa              <  2:5726409/150‑1 (MQ=255)
                                                                                                                                          ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc             >  1:2065773/1‑150 (MQ=255)
                                                                                                                                           taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCACGCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt            >  1:3370283/1‑150 (MQ=255)
                                                                                                                                           taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt            >  2:5573518/1‑150 (MQ=255)
                                                                                                                                           taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt            >  2:3422798/1‑150 (MQ=255)
                                                                                                                                             aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAACGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAACTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt          <  2:1829435/150‑1 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc         >  1:3961289/1‑150 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc         >  2:5026120/1‑150 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc         >  1:3007658/1‑150 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc         <  2:1638950/150‑1 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc         <  2:3115728/150‑1 (MQ=255)
                                                                                                                                              aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCATGATAGAATTGCGGTATTGACTATc         >  1:6013243/1‑150 (MQ=255)
                                                                                                                                               ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa           >  2:839422/1‑150 (MQ=255)
                                                                                                                                               ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa        >  1:7061/1‑150 (MQ=255)
                                                                                                                                               ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa        >  1:1755206/1‑150 (MQ=255)
                                                                                                                                                taatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaa       <  1:4255274/150‑1 (MQ=255)
                                                                                                                                                 aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa      <  2:1755132/150‑1 (MQ=255)
                                                                                                                                                    tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc   <  1:5932191/150‑1 (MQ=255)
                                                                                                                                                     cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc  <  2:875580/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTT‑‑‑CTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCC  >  NC_000913/2470278‑2470576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: