| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,581 | A→C | 22.6% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,581 | 0 | A | C | 22.6% | 203.9 / 34.3 | 142 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base A (46/62); new base C (2/32); total (48/94) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 147 total aligned reads displayed.
CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164432‑164730 | cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:2617888/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:2614896/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGACTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:4011043/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGAGATATCCCAAACATGTCTGGCCGGACGACACGGCAAATACTGCACCGACGAGACGGACGAAAAAGAATTCGTAAGGTTGTTATACTCCCACTCCCTGTGGGAGAGGGCCTGGGAGAGGGCaa > 1:3701325/1‑149 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCCCCCCGGTCGGGCCGGACGACCCGGCAAATACTGCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 2:6098997/150‑1 (MQ=38) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa < 2:6627968/150‑1 (MQ=255) tggTGCAAAAATATATGAATGGGCGTTATCCCAAACTTGTGTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCTTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGTGGGCATCAgc < 2:3990575/149‑1 (MQ=37) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc > 1:1100840/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 1:2872497/1‑150 (MQ=255) aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:5104648/1‑150 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACgg > 1:6518762/1‑149 (MQ=255) agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGttg > 2:732680/1‑149 (MQ=255) gtgaTGAACGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtca < 2:1038553/148‑1 (MQ=255) gagaTGAATGGGCGTTATCCCAAACAGGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTTAGGTTGGTTTTGTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtca < 2:4448866/150‑1 (MQ=38) gagaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCCCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtca < 2:3142659/150‑1 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGtggcccc > 1:241871/1‑144 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTGTTCTCCCCCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCCGCGCGCCCGtgggccc > 2:6369418/1‑144 (MQ=38) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggcccc > 2:6397875/1‑143 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacccac > 2:4242859/1‑148 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGATGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccaca > 2:4924802/1‑150 (MQ=255) atgggCGTTATCCCAAACATGTCTGGCCGTACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac < 2:5638487/148‑1 (MQ=255) gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccaccccc > 2:6153194/1‑150 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTaa < 2:2812880/150‑1 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCaaaac > 2:3431119/1‑148 (MQ=255) tCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCGAAcccac > 1:6779710/1‑148 (MQ=255) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCCCCCCCCAACCcact > 2:4911979/1‑150 (MQ=38) ttttCTGGCCGGACGACCGGGCAAATCCTGCACCTACGCTAGGTACGAAAATTTTTTGGTATGTTTGTTTTTCTCCCTGTCCCTGTGGGTGTGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:2209790/147‑1 (MQ=255) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCGCTCCCGCaa > 2:2804900/1‑150 (MQ=38) ggTCGGGCCGGACGACCCGGCAACTCCGGCACCGCCGCGACGGCCGAAAAAGTATTGGTACGGTGGGTTTTCTCCCTCGCCGTGTGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3813977/149‑1 (MQ=255) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:5051218/150‑1 (MQ=255) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCAAACACCCACCCACaaacggcag > 1:1295927/1‑143 (MQ=25) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCGAACCCGCTCCCCCCCGGGagag > 2:4297485/1‑150 (MQ=37) cccGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTACTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 2:5613542/149‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGGGCCCCCGCCCCCAAACCCCCACCCCCACGGGCGCggg > 1:6681851/1‑150 (MQ=25) cGGACGACCCGGAAAACACTCCACCGACGCGACGGACTAAAATGTATTCTTAAGGTTGTTTCTCTCCCTCTCCCTGGGGTAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg < 1:4964440/150‑1 (MQ=255) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCACCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 2:3765842/150‑1 (MQ=255) gacgacCCGGAAAATACTGCACCGACGCGAGGGACTAAAATTTATTCGTATGTTTGTTTTACTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:1524928/150‑1 (MQ=255) ccgccccGGCAAATACGCCCCCCCCGCGACGCCCGACACAGTACTCGTAAGGTGGGTTGTCGCCCCCCCCCGGGGGGCGCGGGCCGGGGTGCGGGCCTCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc < 2:6398420/146‑1 (MQ=255) ccgcaaaaTCCTGCACCGCCGCGACGGACTAAAAAGTTTTCTTATGGTTGGTTTTCTCCCTCTCCCTGGGGGCGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCCATc < 1:4381672/145‑1 (MQ=255) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTCTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCAAACCCACACCCACCAGGGCGAggggggccatc > 2:3113986/1‑143 (MQ=25) ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGGACCCCCACCCCCACCCACGCCCGACAGGGGGGGGGGGCCgcgc > 1:912170/1‑147 (MQ=21) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGAACCGCACCCCAACCCTCAACCGCCAGGGGGGGGGGACCCAtag > 1:6177423/1‑148 (MQ=25) tGCACCGACGCGATGGACGAAAAAGTATACGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCGCCCAAACCCAAAAACACTCCCACAAGTGGGGGGGGACCCCTCGAGccccaaat > 2:368846/1‑143 (MQ=16) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCCCCCCAACCCCCGCCCGCAAGGGTGAGGGGGCCCAGGCAGCACAAAtt > 2:556607/1‑150 (MQ=25) ccccGACGCGCGGGCCGAAAATGTATTCGTATGGTTGGTTTTCTCCCTCTCGGTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:4176319/148‑1 (MQ=255) cccGACGCGCGGGACTAAAATGTTTCGGTAGGGTGGGTTTTCCCCCTCCCCGGGGGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTg < 2:1225123/149‑1 (MQ=255) ccgccgCGCCGGCCGAAAATTTATTCGTATGGTTGTTTTTCTCCCTCGCCCTGTGGGAGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2708679/150‑1 (MQ=255) ccgccgCGACGGACGAAAAAGTTTTCGTATGGTTGGTTTCCCCCCCCTCCCGGTGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2708689/150‑1 (MQ=255) ccGACGCGCGGGACTAAAATTTATTGGTTGGGTTGGTTTTCTCCCTCCCCCGGGGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:5281446/150‑1 (MQ=255) ccGACGCGCCGGAGGAAACAGTTGTCGTAGGGTTGTTTTTCTCCCGCTCCCTGTGGTTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3447454/150‑1 (MQ=255) ccGACGCGAGGGACGAAAAATTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2708831/150‑1 (MQ=255) ccGACGCGACGGACTAAAATTTTTTCGTATGGTTGGTTTTCGCCCTCTCCCTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:6621754/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:1092879/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCGCCCTCTCCCGGTGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:907964/150‑1 (MQ=255) cgAAAACGTATCGGTCCGGTTGGTTTTCTCCCTCGCCGTGTGGGAGCGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCCCCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt < 1:3102623/150‑1 (MQ=38) gAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCAACCCCCAAACCACACCCCAAAGGGAGAGGGGACCCAACGAGCGCCAAATTTGATAGaaataataaac > 2:1119815/1‑143 (MQ=16) aaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCAACCACACCCCCACCCACACCCACCCCGGCGGGGGGCCCCAGCGAGCCCAAAAATTGATAGCTAtcttcatagg > 1:6134513/1‑145 (MQ=16) atgTATTCGTACGGTTGGTTTCCCCCCTCCCCCGGGGGGTGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:3637765/148‑1 (MQ=255) tttttCTTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:3431119/147‑1 (MQ=38) gTATTCGTAAGGTTGGTTTTCCCCCGCGCCCGGTGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:4813732/150‑1 (MQ=255) tATTCGTATGGTTGGTTTTCTCCCACTCCCGGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:1393055/150‑1 (MQ=255) aTTCGTACGTTTGTTTTTCCCCCCCTCCCTGTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 1:5298138/150‑1 (MQ=255) cGTACGGTGTGTTTTCACCGTCTCCCTGTGTTTGCGGGCCGGGTTGTGGGCCTCAGCGCGCTCGTTCACCCTCACCCTATCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTacc < 2:2925239/150‑3 (MQ=255) tAAGGTTGGTTTTCTCCCTCTCCGTGTGGGTGCGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGTGGTCCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag < 2:2057805/150‑1 (MQ=255) tAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGAACCACACCCAAAACCAAAACCACCAGGGGGAGGGGGCCGAAAGAGCACACAATTTTAGAGATATCATATTCGGTGATGTAACag > 2:6753391/1‑150 (MQ=12) tcggttggttTTCCCCCTCTCCGTGTGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:5773294/148‑1 (MQ=255) ctggttggttTTCACCCTCGCCGTGTGGGCGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:5773315/148‑1 (MQ=255) gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 2:6710808/150‑1 (MQ=255) ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGACATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 1:6449257/150‑1 (MQ=255) tggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 2:3359819/150‑1 (MQ=255) ggttTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:3191513/150‑1 (MQ=255) ttgcccccctctccgtGGGGGTGTGGGCGGGGTTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑Ga < 1:2516105/144‑1 (MQ=255) tctccctctcccTGTGGGAGAGGGCCGGGGTGAGGGCATCGGCGCGCACGTGCACCCCCACCCCAAACCACGACCCCCAGGGAGAGGGGGCCGCTCGCGCACAAAATTTGGGTGATATGGTATTCGGTCTTGTAACGGAAGAACA‑Gagaa > 2:1568859/1‑150 (MQ=16) tccctctccctGGGGTTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATc < 1:5473923/150‑1 (MQ=255) cctctccctGTGGGAGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCgg < 1:5026851/150‑1 (MQ=255) cctctcccaCAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTccc < 2:4983338/71‑1 (MQ=25) tctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCTCTCCCGCAAGGGCGAGGGGCCCGATCGAGCACAAAATTTGGGAGATATATTATTATGTCTTGTAAAAGAAGAACA‑GAAAAACCGGc > 2:1602668/1‑150 (MQ=25) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACAACCACAAGGGGGGGGGGCCCCATCGAGCACAAAATTTGGGAGATATATTATTAAGTATTGTTACACAAGAAAA‑GAAAAACGGGcgt > 2:3835433/1‑148 (MQ=16) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCCACCCAAAACCCCACCCCCCAGGGCGGGGGGCCCCCGCGAGCCCAAATTTTGGGTGATAAGTTCTTATGTGGTGTAACAGAAGAACACGaaaaagggcgt > 2:3268650/1‑143 (MQ=16) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGCCCCCCACCCGAAACCACACCCACCACGGGGGGGGGGGCGAGCGAGCACAAATTTTGTGTGATGTATTATTATGTCTGGTAACAGAAGAACA‑GAAAAAAGGGcgt > 2:3364224/1‑148 (MQ=16) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACTCCCACAAGGGAGAGGGGGCCCATCGAGCACAAATTTTTAGAGATAAATTCTTATGTGTTTTAACAGAATAACA‑GAAAATCGGGCtttttc > 2:3988397/1‑148 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCGCCCGCACCCGAAACCACGCCCACAAGGGGGGGGGGACCCATAGAGCCCAAAATTTGTGGTGTATGATAATGTGTGGTGTGACACAAGAAAA‑GAAAAACGGGCGTTTgggc > 2:474988/1‑150 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTgcgc < 2:6626478/150‑1 (MQ=255) gggtgtgggCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGcc < 1:1249053/144‑1 (MQ=255) ggtgtgggCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCt < 1:4138803/145‑1 (MQ=255) ggAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCt < 2:4213785/150‑1 (MQ=255) ggggCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAat < 1:4242859/149‑1 (MQ=255) cgggggggCGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTg < 2:5712369/148‑1 (MQ=255) cGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:2593539/150‑1 (MQ=255) cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:6570135/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:2022569/150‑1 (MQ=255) ggggTGCGGGCATCCGCGCGCACGGTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:6074169/150‑1 (MQ=255) ggggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGCTATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4699597/150‑1 (MQ=255) ggTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:5753495/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:5753335/150‑1 (MQ=255) gTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGgag < 2:6270103/150‑1 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:2033530/150‑1 (MQ=255) ggCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:1999485/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:726673/150‑1 (MQ=255) aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa < 2:434100/150‑1 (MQ=255) | CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACA‑GAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA > NC_000913/164432‑164730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |