BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,907,998	A→T	7.2%	intergenic (‑57/+31)	eno ← / ← pyrG	enolase/CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,907,998	0	A	T	7.2%	408.4 / 17.7	134	intergenic (‑57/+31)	eno/pyrG	enolase/CTP synthetase
Reads supporting (aligned to +/- strand): ref base A (64/58); new base T (5/5); total (69/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 134 total aligned reads displayed.

AAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAA  >  NC_000913/2907850‑2908142

acaccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCAAGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:1828092/148‑1 (MQ=255)

aaaCCACCCTCCAGATGTACTTCGGCTTCAACCGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:923753/150‑1 (MQ=255)

aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:923884/150‑1 (MQ=255)

aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:1879137/150‑1 (MQ=255)

aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:473406/150‑1 (MQ=255)

aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:1084124/150‑1 (MQ=255)

aaaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAg                                                                                                                                                 <  2:1084126/150‑1 (MQ=255)

 aaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg                                                                                                                                                <  2:1065309/150‑1 (MQ=255)

 aaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg                                                                                                                                                >  1:1143793/1‑150 (MQ=255)

 aaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg                                                                                                                                                >  1:785094/1‑150 (MQ=255)

 aaCCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAgg                                                                                                                                                <  2:291546/150‑1 (MQ=255)

    caccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTa                                                                                                                                             >  1:259589/1‑150 (MQ=255)

     accCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAc                                                                                                                                            >  1:675067/1‑150 (MQ=255)

     accCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAc                                                                                                                                            >  2:310291/1‑150 (MQ=255)

       ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgc                                                                                                                                          <  2:1229810/150‑1 (MQ=255)

       ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgc                                                                                                                                          <  2:1572703/150‑1 (MQ=255)

       ccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgc                                                                                                                                          <  2:1229633/150‑1 (MQ=255)

       ccTCCAGATGTACTTCGGCTTAAACAGTCGGGTTACCACGTGAGTCGATGATTTCACGACCGATGATTTTTACTATTTTGGACATTAGGTTTTCATCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgc                                                                                                                                          <  1:1343311/150‑1 (MQ=255)

          ccAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt                                                                                                                                       <  2:652494/150‑1 (MQ=255)

          ccAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGtt                                                                                                                                       <  2:1842727/150‑1 (MQ=255)

           cAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTg                                                                                                                                      >  1:525234/1‑150 (MQ=255)

            aGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGc                                                                                                                                     <  2:2329068/150‑1 (MQ=255)

                gTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACACCGCGGACCCAGGGTACGCGTTGCCGct                                                                                                                                 <  2:553825/150‑1 (MQ=255)

                gTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGct                                                                                                                                 <  2:194837/150‑1 (MQ=255)

                     tCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAAc                                                                                                                            >  2:2342475/1‑150 (MQ=255)

                     tCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAAc                                                                                                                            >  1:1567338/1‑150 (MQ=255)

                      cGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACt                                                                                                                           >  2:276512/1‑150 (MQ=255)

                          ttCACCAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttttt                                                                                                                       <  2:2023471/150‑1 (MQ=255)

                          ttCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttttt                                                                                                                       <  2:2023474/150‑1 (MQ=255)

                          ttCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttttt                                                                                                                       <  2:1521816/150‑1 (MQ=255)

                           aaaattgTCGGTTTACCAGGTGTGTCTATTTTTTCACGACCTTTTATTTTTACTATTTTGGAAATTTGTTTTTCATAATGTAAATTTTTAAAATTAAACTACAGAAAAACACCGGGTACCCTGGTTACGCGTTGCCGCTCTAACtttttt                                                                                                                      <  1:2046333/144‑1 (MQ=255)

                             aaCAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACACCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCGCTAACTTTTttac                                                                                                                    >  1:2141953/1‑150 (MQ=255)

                                 gTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTttacttac                                                                                                                <  2:2124621/150‑1 (MQ=255)

                                  tCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTGTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTCACTTTTTtacttact                                                                                                               >  2:1939290/1‑150 (MQ=255)

                                       ttACCACGGTTGTCTATGATTTCACGACGGATGATTTTTACGTTTTTGTACTTTAGTTTTTCCTCATTTCACTTGTTAAACTTAAACTCCAGAAAAACACCGCGTACCCAGGGTAGGCGTTGCCGCTCTAACTTTTTTACTTACTTCGcc                                                                                                          <  1:921890/150‑1 (MQ=255)

                                        taagaCGGGAGTCGATGATTTCACGACGGATGATTTTTACGATTTTGGTCATTAGGTTTTCCTCAAGTCACTAGTTAATCTGAAACTCCTGTCAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTTCTTACTTCGCCt                                                                                                         <  2:1451567/146‑1 (MQ=255)

                                         aCCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTg                                                                                                        <  2:1392756/150‑1 (MQ=255)

                                           cACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGAc                                                                                                      <  2:346328/150‑1 (MQ=255)

                                            aCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACg                                                                                                     >  2:2245895/1‑150 (MQ=255)

                                            aCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACg                                                                                                     >  2:152000/1‑150 (MQ=255)

                                            aCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCGCTAACTTTTTTACTTACTTCGCCTGACg                                                                                                     >  2:2245902/1‑150 (MQ=255)

                                                  gTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGTTTTTCCTCAAGTCACTTTTTAAACTGAAACTCCAGACAAACAACGCGTACCCCGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg                                                                                               <  1:737521/150‑1 (MQ=255)

                                                     gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGATGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGaa                                                                                            >  2:2204851/1‑150 (MQ=255)

                                                          tttCACGACCGATGATTTTTACGATTTTGGACATAAGGTTTTCCTCAAGTCACTAGTTAAACTGAAAATCCAGACAAACAACGAGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAAATCGc                                                                                       >  1:1806526/1‑150 (MQ=255)

                                                           ttCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCt                                                                                      <  1:1523122/150‑1 (MQ=255)

                                                               cGACCGTTGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcg                                                                                  <  1:1134579/150‑1 (MQ=255)

                                                               cGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcg                                                                                  <  2:32201/150‑1 (MQ=255)

                                                               cGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcg                                                                                  <  1:2352349/150‑1 (MQ=255)

                                                               cGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcg                                                                                  <  1:2352323/150‑1 (MQ=255)

                                                                  ccGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt                                                                               <  2:2159731/150‑1 (MQ=255)

                                                                  ccGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt                                                                               >  2:1178973/1‑150 (MQ=255)

                                                                    gatgatTTTTACGATTTTCGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGCTACGCGAAGCCGCACTAACTATATAACTAACATCGCCTGACGTTTCTGGAACTCGCTGGCGGCtat                                                                             >  2:1707789/1‑148 (MQ=255)

                                                                            ttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACACCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGc                                                                     <  2:526258/150‑1 (MQ=255)

                                                                            ttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCCGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGc                                                                     <  1:1089276/150‑1 (MQ=255)

                                                                             tACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGcc                                                                    >  2:1055736/1‑150 (MQ=255)

                                                                             tACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGcc                                                                    >  1:1119240/1‑150 (MQ=255)

                                                                             tACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGcc                                                                    >  1:2138698/1‑150 (MQ=255)

                                                                             tACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGcc                                                                    >  1:780153/1‑150 (MQ=255)

                                                                              aCGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCt                                                                   <  1:1125303/150‑1 (MQ=255)

                                                                               cGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg                                                                  <  1:2342475/150‑1 (MQ=255)

                                                                                  ttttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCaa                                                               >  1:2152249/1‑150 (MQ=255)

                                                                                   tttGGACATTAGGTGTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCaaa                                                              <  1:2279141/150‑1 (MQ=255)

                                                                                         cATTCGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg                                                        <  1:310291/150‑1 (MQ=255)

                                                                                             aGGTTTTCCTCAAGTCACTATTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGa                                                    <  1:1855439/150‑1 (MQ=255)

                                                                                             aGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGGACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGa                                                    <  1:1963664/150‑1 (MQ=255)

                                                                                                ttttCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCa                                                 >  2:1796130/1‑150 (MQ=255)

                                                                                                      tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGt                                           >  1:128991/1‑150 (MQ=255)

                                                                                                      tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGt                                           >  1:1919726/1‑150 (MQ=255)

                                                                                                      tCAAGTCACGAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGt                                           <  1:1245340/150‑1 (MQ=255)

                                                                                                           tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGt                                      >  2:1316092/1‑150 (MQ=255)

                                                                                                               tAGTTCAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACGTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGaa                                  <  2:1600874/150‑1 (MQ=255)

                                                                                                                 gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt                                >  1:2280356/1‑150 (MQ=255)

                                                                                                                 gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGt                                >  1:2280344/1‑150 (MQ=255)

                                                                                                                     aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAAc                            <  1:2283905/150‑1 (MQ=255)

                                                                                                                     aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAAc                            <  1:2283857/150‑1 (MQ=255)

                                                                                                                     aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAAc                            <  1:685977/150‑1 (MQ=255)

                                                                                                                        tGAAACTCCAGACAAACAACGCGTACCCAGGGTCCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTcc                         >  2:178002/1‑150 (MQ=255)

                                                                                                                        tGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTcc                         >  2:1608251/1‑150 (MQ=255)

                                                                                                                           aaCTCCAGACACCCACCGCGTACCCAGGGCACGCGACGCCGCCCTAACTAATTCACTCACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa                      <  1:2091257/150‑1 (MQ=255)

                                                                                                                           aaCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa                      >  2:248479/1‑150 (MQ=255)

                                                                                                                             cTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATg                    <  2:675067/150‑1 (MQ=255)

                                                                                                                              tCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATgg                   >  1:1993804/1‑150 (MQ=255)

                                                                                                                               ccAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGa                  >  1:2317573/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  1:1781362/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  1:1165707/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                <  2:2146428/150‑1 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  2:1874147/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  1:710796/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  1:439930/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  1:664046/1‑150 (MQ=255)

                                                                                                                                 aGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGGTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACCGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAAc                >  2:1874140/1‑150 (MQ=255)

                                                                                                                                  gACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACt               >  1:1306420/1‑150 (MQ=255)

                                                                                                                                   acaaccaaCGCGTACCCAGGGGACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTg              <  2:1778296/150‑1 (MQ=255)

                                                                                                                                       gcaacGCGTACCCTGGGTACGCGTTGTCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCaa          >  2:675197/2‑150 (MQ=255)

                                                                                                                                       acaacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCaa          >  2:289654/1‑150 (MQ=255)

                                                                                                                                          acGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGcc       >  1:381546/1‑150 (MQ=255)

                                                                                                                                            gcgTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCAc     >  2:200315/1‑150 (MQ=255)

                                                                                                                                            gcgTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCAc     >  2:1992350/1‑150 (MQ=255)

                                                                                                                                              gTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGa   >  2:1426718/1‑150 (MQ=255)

                                                                                                                                               tACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGaa  <  1:1923687/150‑1 (MQ=255)

AAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAA  >  NC_000913/2907850‑2908142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`