Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,042,418 A→T 5.5% intergenic (+125/+71) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,042,4180AT 5.5% 706.3 / 22.3 199intergenic (+125/+71)ygfZ/yqfAiron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (95/93);  new base T (3/8);  total (98/101)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 199 total aligned reads displayed.

TGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGC  >  NC_000913/3042271‑3042566
                                                                                                                                                   |                                                                                                                                                    
   cGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCg                                                                                                                                                 <  2:2035722/150‑1 (MQ=255)
    gTATTCGCTCGAAGATTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCga                                                                                                                                                <  1:650346/150‑2 (MQ=38)
    gTATTCGCTCGAAGAGTAACTCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCga                                                                                                                                                <  1:2397440/150‑2 (MQ=38)
    gTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCga                                                                                                                                                <  1:2326964/150‑2 (MQ=39)
    gTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCga                                                                                                                                                <  1:2184285/150‑2 (MQ=39)
    gTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCga                                                                                                                                                <  2:1363988/150‑2 (MQ=39)
    gTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATTCGGCGTGAACGCCTTATCCga                                                                                                                                                <  2:1675939/150‑2 (MQ=39)
    gTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCgg                                                                                                                                                <  2:388538/150‑1 (MQ=255)
    gTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCgg                                                                                                                                                <  1:771867/150‑1 (MQ=255)
     tATTCGCTCGAAGATTAAATCTCTTTATCGCATCAGGCATTTAGCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGc                                                                                                                                               <  1:721921/150‑1 (MQ=255)
         cGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGtt                                                                                                                                           <  2:2143546/150‑1 (MQ=255)
         cGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGtt                                                                                                                                           <  2:222248/150‑1 (MQ=255)
              gAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCt                                                                                                                                      >  2:798678/1‑150 (MQ=255)
              gAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCt                                                                                                                                      <  2:1081067/150‑1 (MQ=255)
                  agTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCTGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGa                                                                                                                                  >  1:912961/1‑150 (MQ=255)
                  agTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGa                                                                                                                                  >  1:478618/1‑150 (MQ=255)
                      aaTCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGcc                                                                                                                              <  2:381283/150‑1 (MQ=255)
                       aTCTCTTTATCGCATCAGGCTTTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCt                                                                                                                             <  1:2086084/150‑1 (MQ=255)
                        tctcTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTg                                                                                                                            >  2:358774/1‑150 (MQ=255)
                          tctTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGAt                                                                                                                          >  1:1880164/1‑150 (MQ=255)
                           ctTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCt                                                                                                                             >  1:682297/1‑146 (MQ=255)
                           ctTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCt                                                                                                                             <  2:682297/146‑1 (MQ=255)
                                  gCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt                                                                                                                  <  2:1547918/150‑1 (MQ=255)
                                      cAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcg                                                                                                              >  1:2187715/1‑150 (MQ=255)
                                       aGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcgc                                                                                                             <  1:84656/150‑1 (MQ=255)
                                       aGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcgc                                                                                                             <  1:1224723/150‑1 (MQ=255)
                                       aGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGgcgc                                                                                                             >  2:2257734/1‑150 (MQ=255)
                                        ggCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGcgcg                                                                                                            >  1:1479714/1‑150 (MQ=255)
                                         gCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGAGAAGGCGTTCACGCCGACTCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGt                                                                                                           >  1:2381050/1‑150 (MQ=255)
                                          cATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                                                                          <  2:920202/150‑1 (MQ=255)
                                          cATTTATCGCCTGATGCGACGCTGGCGCGTATTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTc                                                                                                          <  1:556747/150‑1 (MQ=255)
                                              tATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                      <  1:1548040/150‑1 (MQ=255)
                                              tATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTGATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                      <  2:1775557/150‑1 (MQ=255)
                                               aTCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                     >  2:1294065/1‑150 (MQ=255)
                                               aTCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                     >  2:157556/1‑150 (MQ=255)
                                               aTCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                     <  1:598998/150‑1 (MQ=255)
                                                tCGCCTGTTGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                    <  2:1312740/150‑1 (MQ=255)
                                                tCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                    >  1:1537611/1‑150 (MQ=255)
                                                tCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                                                    >  1:1662076/1‑150 (MQ=255)
                                                 cGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACGACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                   <  1:2048044/150‑1 (MQ=255)
                                                 cGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                   <  1:947502/150‑1 (MQ=255)
                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt                                                                                                                  >  1:585688/1‑133 (MQ=255)
                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCt                                                                                                                  <  2:585688/133‑1 (MQ=255)
                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                 >  2:1952508/1‑150 (MQ=255)
                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                 <  2:1127954/150‑1 (MQ=255)
                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                 >  2:297064/1‑150 (MQ=255)
                                                   ccTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                 <  2:1618088/150‑1 (MQ=255)
                                                   ccTGAGGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGc                                                                                                 <  1:2309540/150‑1 (MQ=255)
                                                        tGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa                                                                                            >  2:490256/1‑150 (MQ=255)
                                                         gCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaa                                                                                           >  2:2074678/1‑150 (MQ=255)
                                                          cGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaaa                                                                                          <  2:1527200/150‑1 (MQ=255)
                                                          cGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaaa                                                                                          <  1:1091628/150‑1 (MQ=255)
                                                          cGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACaaa                                                                                          <  1:395050/150‑1 (MQ=255)
                                                           gACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAg                                                                                         >  1:2337758/1‑150 (MQ=255)
                                                              gCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATCAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCa                                                                                      <  2:2388106/150‑1 (MQ=255)
                                                              gCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCa                                                                                      >  2:1933717/1‑150 (MQ=255)
                                                               cTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCAt                                                                                     >  2:2291282/1‑150 (MQ=255)
                                                                 ggCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAc                                                                                   <  2:2200884/150‑1 (MQ=255)
                                                                 ggCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAc                                                                                   <  2:2178669/150‑1 (MQ=255)
                                                                  gcgcGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATAcc                                                                                  >  1:1043872/1‑150 (MQ=255)
                                                                    gcgTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCa                                                                                >  1:1529167/1‑150 (MQ=255)
                                                                      gTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAtt                                                                              <  1:2156479/150‑1 (MQ=255)
                                                                      gTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAtt                                                                              <  1:2252777/150‑1 (MQ=255)
                                                                      gTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCAtt                                                                              <  1:1629050/150‑1 (MQ=255)
                                                                      gTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGAAGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACACAtt                                                                              <  2:1118302/150‑1 (MQ=255)
                                                                           aTCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGcc                                                                         >  1:1430442/1‑150 (MQ=255)
                                                                            tCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCt                                                                        >  2:474481/1‑150 (MQ=255)
                                                                                   tGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAat                                                                 >  2:1985233/1‑150 (MQ=255)
                                                                                     ggATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAatat                                                               >  1:1804210/1‑150 (MQ=255)
                                                                                       aTTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATAc                                                             <  2:121060/150‑1 (MQ=255)
                                                                                                 tttATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCg                                                   >  1:1959385/1‑150 (MQ=255)
                                                                                                  ttATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGc                                                  >  2:858010/1‑150 (MQ=255)
                                                                                                   tATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGcc                                                 <  2:1430887/150‑1 (MQ=255)
                                                                                                     tCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAg                                               >  1:349782/1‑150 (MQ=255)
                                                                                                       acaacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAATGGCATACCCATTACGCCTGCCCAATATTCTAATAGATCGCCAGaa                                             >  2:1969633/1‑150 (MQ=255)
                                                                                                       acaacaGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGaa                                             >  2:394520/1‑150 (MQ=255)
                                                                                                              ggACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGca                                      <  1:2257734/150‑1 (MQ=255)
                                                                                                                 cGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGcacac                                   <  2:2010653/150‑1 (MQ=255)
                                                                                                                  gAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGcacaca                                  <  1:994967/150‑1 (MQ=255)
                                                                                                                  gAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGcacaca                                  <  1:65920/150‑1 (MQ=255)
                                                                                                                  gAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGcacaca                                  <  1:358818/150‑1 (MQ=255)
                                                                                                                   aGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCacacac                                 <  2:1537611/150‑1 (MQ=255)
                                                                                                                     gCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTa                               >  2:830483/1‑150 (MQ=255)
                                                                                                                     gCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTa                               <  2:727187/150‑1 (MQ=255)
                                                                                                                         gTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCACTATACAAATAGATCGCCAGAAAGTGGCACACACTAccgc                           >  1:388831/1‑150 (MQ=255)
                                                                                                                            tgccGGATTCGGCGTGAACGCCTTATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGa                        >  2:1742404/4‑150 (MQ=37)
                                                                                                                            gATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGa                        <  1:798678/150‑1 (MQ=255)
                                                                                                                             gccGGATTCGGCGTGAACGCCTTATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAg                       >  2:818002/3‑150 (MQ=38)
                                                                                                                             aTCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAg                       >  1:559593/1‑150 (MQ=255)
                                                                                                                              tCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGc                      >  1:513904/1‑150 (MQ=255)
                                                                                                                              tCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGc                      >  2:1877993/1‑150 (MQ=255)
                                                                                                                              tCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGc                      >  1:579578/1‑150 (MQ=255)
                                                                                                                                     aGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGc               >  1:1085538/1‑150 (MQ=255)
                                                                                                                                       gCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCg             >  1:1891204/1‑150 (MQ=255)
                                                                                                                                       gCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCg             >  1:1463150/1‑150 (MQ=255)
                                                                                                                                       gCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCg             >  1:1296088/1‑150 (MQ=255)
                                                                                                                                       gCGTTCACACCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCg             >  2:2328827/1‑150 (MQ=255)
                                                                                                                                             aCGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAg       >  1:81218/1‑150 (MQ=255)
                                                                                                                                              cGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGa      <  2:655453/150‑1 (MQ=255)
                                                                                                                                                  gAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGc  <  2:1633800/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGCCGTATTCGCTCGAAGAGTAAATCTCTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTCACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGCACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGC  >  NC_000913/3042271‑3042566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: