Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,092,842 G→A 5.8% intergenic (+14/‑95) gshB → / → yqgE glutathione synthetase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,092,8420GA 5.8% 476.6 / 12.1 138intergenic (+14/‑95)gshB/yqgEglutathione synthetase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (70/60);  new base A (4/4);  total (74/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 140 total aligned reads displayed.

GATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGAT  >  NC_000913/3092694‑3092989
                                                                                                                                                    |                                                                                                                                                   
gATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCgaga                                                                                                                                                    >  1:927616/1‑150 (MQ=255)
      atcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtc                                                                                                                                              >  2:1943105/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtct                                                                                                                                             >  2:943341/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtct                                                                                                                                             >  2:1059554/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAAAAGGAtct                                                                                                                                             >  2:597258/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAAAAGGAtct                                                                                                                                             >  2:327309/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAAAAGGAtct                                                                                                                                             >  2:327330/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAAAAGGAtct                                                                                                                                             >  2:327289/1‑150 (MQ=255)
       tcGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGACATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtct                                                                                                                                             >  2:2236851/1‑150 (MQ=255)
        cGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtctc                                                                                                                                            <  2:1032766/150‑1 (MQ=255)
        cGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGAtctc                                                                                                                                            <  1:324410/150‑1 (MQ=255)
         ggCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCg                                                                                                                                           >  1:174557/1‑150 (MQ=255)
              ccGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTgag                                                                                                                                      <  1:689183/150‑1 (MQ=255)
               cGTCTGACTGAACTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTgaga                                                                                                                                     <  2:2283487/150‑1 (MQ=255)
               cGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTTGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTgaga                                                                                                                                     <  1:1594507/150‑1 (MQ=255)
               cGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTgaga                                                                                                                                     <  2:2283505/150‑1 (MQ=255)
               cGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTgaga                                                                                                                                     <  2:112327/150‑1 (MQ=255)
                 tCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGAct                                                                                                                                   >  1:254487/1‑150 (MQ=255)
                   tgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGActct                                                                                                                                 >  2:346681/1‑150 (MQ=255)
                   tgactgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTAAAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGActct                                                                                                                                 >  1:1333116/1‑150 (MQ=255)
                       tgaAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGt                                                                                                                             >  1:4157/1‑150 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATTTTAATGGATGCCATAGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          >  2:2390849/1‑150 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          >  1:412522/1‑150 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          >  1:782560/1‑150 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          >  1:731335/1‑150 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          <  1:1757272/150‑1 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          >  2:2055960/1‑150 (MQ=255)
                          aaTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGAc                                                                                                                          <  2:2004581/150‑1 (MQ=255)
                           aTTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACa                                                                                                                         >  1:1990426/1‑150 (MQ=255)
                            ttAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAg                                                                                                                        <  1:1569265/150‑1 (MQ=255)
                                cGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGcc                                                                                                                    <  2:2099268/150‑1 (MQ=255)
                                       aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                                                                                             <  2:497668/150‑1 (MQ=255)
                                       aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                                                                                             <  1:2351272/150‑1 (MQ=255)
                                       aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                                                                                             >  2:683306/1‑150 (MQ=255)
                                       aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                                                                                             >  2:1219630/1‑150 (MQ=255)
                                       aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                                                                                             <  2:1421052/150‑1 (MQ=255)
                                       aGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCttt                                                                                                             >  2:1641260/1‑150 (MQ=255)
                                        gCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTc                                                                                                            >  1:1261379/1‑150 (MQ=255)
                                        gCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTATACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTc                                                                                                            >  2:177069/1‑150 (MQ=255)
                                          ccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                                                                                                          >  2:2311509/1‑150 (MQ=255)
                                          ccAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCa                                                                                                          <  1:1128965/150‑1 (MQ=255)
                                            aaCCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACg                                                                                                        <  1:30335/150‑1 (MQ=255)
                                                    ttCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTgg                                                                                                <  1:1943105/150‑1 (MQ=255)
                                                        tGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGgcgc                                                                                            <  2:1231681/150‑1 (MQ=255)
                                                         gagaTTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCt                                                                                           <  1:1902661/150‑1 (MQ=255)
                                                          agaTTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTg                                                                                          >  2:427380/1‑150 (MQ=255)
                                                             ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTg                                                                                       >  2:7279/1‑150 (MQ=255)
                                                             ttGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTg                                                                                       >  1:1006189/1‑150 (MQ=255)
                                                                aaGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCtt                                                                                    <  2:2215884/150‑1 (MQ=255)
                                                                     gagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTg                                                                               <  2:842876/150‑1 (MQ=255)
                                                                     gagTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTg                                                                               <  2:944001/150‑1 (MQ=255)
                                                                       gTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGaa                                                                             <  2:2120540/150‑1 (MQ=255)
                                                                         ttCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAAcc                                                                           <  1:867237/150‑1 (MQ=255)
                                                                            cGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAgg                                                                        >  1:2343693/1‑150 (MQ=255)
                                                                             ggTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGa                                                                       <  1:940798/150‑1 (MQ=255)
                                                                                gtCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAAc                                                                    <  2:674939/150‑1 (MQ=255)
                                                                                  cGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg                                                                  >  2:555995/1‑150 (MQ=255)
                                                                                  cGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg                                                                  <  1:1001538/150‑1 (MQ=255)
                                                                                  cGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg                                                                  <  1:268113/150‑1 (MQ=255)
                                                                                  cGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg                                                                  <  1:284845/150‑1 (MQ=255)
                                                                                  cGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAg                                                                  >  2:1368023/1‑150 (MQ=255)
                                                                                    aTCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGaa                                                                >  2:374375/1‑150 (MQ=255)
                                                                                    aTCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGaa                                                                >  2:1491071/1‑150 (MQ=255)
                                                                                         cGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACctct                                                           >  1:1164627/1‑150 (MQ=255)
                                                                                         cGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACctct                                                           >  1:2093482/1‑150 (MQ=255)
                                                                                         cGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACctct                                                           >  2:286921/1‑150 (MQ=255)
                                                                                         cGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACctct                                                           >  2:2307885/1‑150 (MQ=255)
                                                                                         cGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACctct                                                           >  2:2272896/1‑150 (MQ=255)
                                                                                         cGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACctct                                                           >  1:941000/1‑150 (MQ=255)
                                                                                           gAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAGCCAGGAAACAGAACCTCTGa                                                         <  2:885497/150‑1 (MQ=255)
                                                                                              tGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACaa                                                      <  1:92353/150‑1 (MQ=255)
                                                                                              tGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACaa                                                      <  1:2199513/150‑1 (MQ=255)
                                                                                               gTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAAt                                                     >  2:248987/1‑150 (MQ=255)
                                                                                                ttAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATg                                                    >  2:2160400/1‑150 (MQ=255)
                                                                                                ttAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATg                                                    >  2:784639/1‑150 (MQ=255)
                                                                                                        tGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACa                                            >  1:944690/1‑150 (MQ=255)
                                                                                                         gCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAg                                           >  1:20460/1‑150 (MQ=255)
                                                                                                         gCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAg                                           >  2:1999672/1‑150 (MQ=255)
                                                                                                            aTCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCAt                                        >  2:2107627/1‑150 (MQ=255)
                                                                                                            aTCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCAt                                        >  1:292343/1‑150 (MQ=255)
                                                                                                            aTCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCAt                                        >  1:2360793/1‑150 (MQ=255)
                                                                                                            aTCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCAt                                        >  1:723498/1‑150 (MQ=255)
                                                                                                            aTCGAAGCACGTTTACAGCAGCAGTAACCCACCATAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCAGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCAt                                        >  2:1725118/1‑150 (MQ=255)
                                                                                                                         tACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTg                           <  1:1984369/150‑1 (MQ=255)
                                                                                                                         tACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTg                           <  2:4157/150‑1 (MQ=255)
                                                                                                                            agcagcagTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCa                        >  2:1300149/1‑150 (MQ=255)
                                                                                                                            agcagcagTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCa                        >  2:1614223/1‑150 (MQ=255)
                                                                                                                            agcagcagTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCa                        >  2:458489/1‑150 (MQ=255)
                                                                                                                             gcagcagTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCAt                       <  1:1754785/150‑1 (MQ=255)
                                                                                                                              cagcagTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATg                      <  2:1786712/150‑1 (MQ=255)
                                                                                                                                  agTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTg                  >  1:1429860/1‑150 (MQ=255)
                                                                                                                                     aaCCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGctc               >  2:693967/1‑150 (MQ=255)
                                                                                                                                         cACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCCTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCa           <  1:52244/150‑1 (MQ=255)
                                                                                                                                             ttAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGAt       >  2:1554763/1‑150 (MQ=255)
                                                                                                                                             ttAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGCAAACAGAACCTCTGACAATGAATTAACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGAt       >  2:7539/1‑150 (MQ=255)
                                                                                                                                              tAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATc      >  2:2389453/1‑150 (MQ=255)
                                                                                                                                                 cGAAAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGa   <  1:327330/150‑1 (MQ=255)
                                                                                                                                                 cGAAAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGa   <  1:327289/150‑1 (MQ=255)
                                                                                                                                                 cGAAAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGa   <  1:597258/150‑1 (MQ=255)
                                                                                                                                                  gagaAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGAt  >  1:1001453/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GATATCATCGGCGACCGTCTGACTGAAATTAACGTCACCAGCCCAACCTGTATTCGTGAGATTGAAGCAGAGTTTCCGGTGTCGATCACCGGAATGTTAATGGATGCCATCGAAGCACGTTTACAGCAGCAGTAACCCACCTTAGCGAGAAGGATCTCGTTGAGACTCTGAGTGACAGCGCCCTTCTTTCCACGCATACTGGGCGCTGTTGCTTTTTTGAACCAGGAAACAGAACCTCTGACAATGAATTTACAGCATCACTTTCTTATTGCCATGCCTGCTCTCCAGGATCCGAT  >  NC_000913/3092694‑3092989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: