Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,080 C→T 5.5% intergenic (+478/+136) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,0800CT 5.5% 356.7 / 9.3 127intergenic (+478/+136)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (60/60);  new base T (3/4);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 134 total aligned reads displayed.

ATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269931‑3270228
                                                                                                                                                     |                                                                                                                                                    
tttcatCTTTCTGCCACTCCAAAATTAAAAACCCGCCGAAGGGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACTGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                                                                      <  1:1801366/149‑1 (MQ=11)
 ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                                                                     >  1:671222/1‑150 (MQ=255)
    atCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGAGAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAg                                                                                                                                                  >  2:706515/1‑150 (MQ=255)
       tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  1:474228/150‑1 (MQ=37)
       tttCTGCCACTCCAAAAGCAAAAACCCGCCGTATCGGGTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  2:983777/150‑1 (MQ=11)
           tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:1090426/150‑1 (MQ=37)
           tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  2:657652/150‑1 (MQ=37)
           tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  2:313613/150‑1 (MQ=37)
           tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:2115827/150‑1 (MQ=37)
           tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:1873688/150‑1 (MQ=37)
           tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:1387279/150‑1 (MQ=37)
            gCCCCTCCAAAATTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                                                                          <  1:619358/150‑1 (MQ=11)
            gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                                                                          <  1:619368/150‑1 (MQ=255)
             ccccTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                                         <  1:1098223/150‑1 (MQ=37)
              cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                        <  1:507159/150‑1 (MQ=37)
              cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGGCGGGGAAAGTCAGTCAAGGTGGTGCACCGACAAAAGGAAAAACCCGCCGCAGCTGGTTTTTa                                                                                                                                        >  1:175104/1‑150 (MQ=11)
                 tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                     >  1:1000685/1‑150 (MQ=37)
                 tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                     >  1:2191823/1‑150 (MQ=37)
                    aaaaGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGtttttgcttttg                                                                                                                                  <  2:104776/150‑8 (MQ=11)
                          aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGTGTCGGGGACAGGCCATCATCGTGCTGCCCCACCAAAAGCAATAACCCGCCGAAGCGGGGTGTTACGTCAACCAggg                                                                                                                            >  2:1232708/1‑149 (MQ=11)
                              aCCCGCCGAAGCGGGTTTTTACGTAAAACCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                        <  2:1724932/150‑1 (MQ=255)
                               cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                                                       <  1:2094497/150‑1 (MQ=37)
                                  gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                                                                    >  1:1983933/1‑150 (MQ=17)
                                  gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCACCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                                                    <  2:1357485/150‑1 (MQ=37)
                                   ccgCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  1:1301925/150‑1 (MQ=37)
                                   ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  1:340165/150‑1 (MQ=37)
                                   ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  1:1301928/150‑1 (MQ=37)
                                    cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATATTTATGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                                  >  1:1714691/1‑150 (MQ=255)
                                     gAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCg                                                                                                                 <  1:762632/150‑1 (MQ=37)
                                        gCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  1:1245706/150‑1 (MQ=255)
                                        gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  1:2343298/150‑1 (MQ=37)
                                          gggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACAGATAAg                                                                                                            >  1:1386934/1‑150 (MQ=37)
                                           ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                           >  2:542317/1‑150 (MQ=17)
                                           ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                           >  2:1585216/1‑150 (MQ=17)
                                           ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                           >  2:267874/1‑150 (MQ=17)
                                              ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                        <  2:940547/150‑1 (MQ=38)
                                              ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                        <  2:446859/150‑1 (MQ=38)
                                                  aCGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcgttg                                                                                                    >  1:537867/1‑149 (MQ=255)
                                                     tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                 >  2:1629184/1‑150 (MQ=40)
                                                        aaCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  2:1541446/150‑1 (MQ=255)
                                                           aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCCGGTGAAACAGACCGCTAAGCCGCTTTGGTTTGGGtg                                                                                           >  1:1725538/1‑149 (MQ=11)
                                                             gtaaaaCTGACCGATAAGCCGCCTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                         <  1:1925468/147‑1 (MQ=17)
                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCACTCCAAAATCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                         <  1:81230/150‑1 (MQ=11)
                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGTCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCGTTCTTGTGGGtaga                                                                                         >  2:1630750/1‑148 (MQ=11)
                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                         >  2:544021/1‑150 (MQ=37)
                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtcta                                                                                         >  2:1872166/1‑150 (MQ=17)
                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTggggaga                                                                                         >  2:1411234/1‑146 (MQ=12)
                                                             gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGAACGATAATCAGCTTTCTTTTGGGtata                                                                                         >  2:2017963/1‑150 (MQ=11)
                                                              taaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                        <  1:150019/116‑1 (MQ=18)
                                                                    tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTAATCTTTATGCCCCACCAAAATCAAAAAACCCCCCCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTGTAGcgtcgt                                                                                  >  1:713581/1‑150 (MQ=11)
                                                                    tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgg                                                                                  >  2:1325788/1‑149 (MQ=37)
                                                                    tGACCGATAAGCCGCTTTCGTTTGGGTATAGTGTCGTGGACAGTCCATCATCGTTCTGCCCCACCCAAAGCAAAAAACCCCCCAAGCCGGTTTTTACCTAAACCCCGTGAAACTGACCCAAAAGCCGCGTTCGTTTGGGGATAGagtcgg                                                                                  >  1:2191325/1‑149 (MQ=2)
                                                                    tGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGGAAACAAGGTGAAACTGACCGATAAGCCCGGTTCTTTTGGGGTGAGcgtcgt                                                                                  >  1:3606/1‑150 (MQ=11)
                                                                     gcccGATAACCAGCTTTCTTGTGGGTATTGCGCCGTGGTCAGCCATTCATCTCTCTGCCCCTCCAAAATCTAAAACCCGCCGCAGCGGGTTTTTACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                 <  2:886163/148‑1 (MQ=11)
                                                                     gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                 <  1:865248/150‑1 (MQ=37)
                                                                     gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTg                                                                                 >  2:388405/1‑150 (MQ=255)
                                                                            aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                                                          <  1:2310346/150‑1 (MQ=37)
                                                                               ctgCTTTCTTTTTTGTTCTGTGTCGGGGACTGTGTATATTCTTTATGGCCCTCCAAAAGCTTAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  1:1227762/148‑1 (MQ=2)
                                                                               ccGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  1:1227737/150‑1 (MQ=37)
                                                                               ccGCATTCTTTTTGGAATATTGACGTGGACAGTCTTTCATCCTGCTGCCCCTCCAGATGATTAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  2:1926579/150‑1 (MQ=11)
                                                                                         ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCAAGGCATTCATCTTTCtc                                                             >  2:1308574/1‑149 (MQ=11)
                                                                                          tGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTGTGGGTAGAGCGTCGTGGCCAGGCCTTCATCGTTCTGc                                                            >  2:1728246/1‑150 (MQ=11)
                                                                                              tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGAGGAAAGTCAATCATCGTTCTGccccg                                                        >  2:510905/1‑149 (MQ=255)
                                                                                                       gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATAGTTATGCCCCACCAAAAGCa                                               >  2:1447041/1‑150 (MQ=11)
                                                                                                             aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAAATAATGCCCCACCAAAAGCAAAAAcc                                         >  1:153719/1‑150 (MQ=11)
                                                                                                             aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGTGTATAGCGTAGTGGCAAGTCATTAATATTCCAGCACCACCAAAATCAAAAAcc                                         >  1:309105/1‑150 (MQ=11)
                                                                                                               tctttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                       <  2:2191823/147‑1 (MQ=37)
                                                                                                               tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                       <  1:169120/150‑1 (MQ=37)
                                                                                                                 attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                     >  1:1401532/1‑150 (MQ=37)
                                                                                                                 attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                     >  1:2137035/1‑150 (MQ=37)
                                                                                                                    cttCTTTCTGCCACTCCAACATCAAAAACCCGCCGAAGCGGTTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                  <  2:1333812/148‑1 (MQ=11)
                                                                                                                      tCTTTCTGCCCCTCCAACAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAGc                                <  1:1831900/150‑1 (MQ=255)
                                                                                                                            tGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAATCCCGCCGAAGCGGGttt                          <  2:2260783/150‑1 (MQ=255)
                                                                                                                            tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                          <  2:568586/150‑1 (MQ=37)
                                                                                                                            ggCCCCTCCAAAAGCACAAACCCGCCGTAGCGGTTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                          <  2:2260892/149‑1 (MQ=255)
                                                                                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  1:1442709/1‑150 (MQ=37)
                                                                                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  1:384237/1‑150 (MQ=37)
                                                                                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  2:2101577/1‑150 (MQ=37)
                                                                                                                             gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTAGGCCCCACCAAAAGCAAAAACCCCCCGAAGCGGGtttt                         >  2:1652711/1‑150 (MQ=11)
                                                                                                                             gCCCCGCCATCAGCTAAAACCCGCCGCAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                         <  1:1754039/150‑1 (MQ=11)
                                                                                                                               cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGGCCTTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTGTTa                       >  2:1722843/1‑150 (MQ=11)
                                                                                                                                ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                      <  1:489067/150‑1 (MQ=37)
                                                                                                                                ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                      <  1:284838/150‑1 (MQ=37)
                                                                                                                                  tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                    >  2:1072067/1‑150 (MQ=37)
                                                                                                                                     aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTgaa                 >  1:1576/1‑150 (MQ=37)
                                                                                                                                     aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGGTTACGTcaa                 >  2:737846/1‑150 (MQ=255)
                                                                                                                                         gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg             >  2:362818/1‑150 (MQ=38)
                                                                                                                                          taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            >  2:1610356/2‑150 (MQ=255)
                                                                                                                                          taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAAACCCCCGAAGCGGGGTTTTACGTAAATCAgg            >  2:1038988/2‑150 (MQ=11)
                                                                                                                                          taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCCgg            >  1:1361088/2‑150 (MQ=11)
                                                                                                                                          cacaaaCCCGCCGTAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            <  1:2241420/147‑1 (MQ=255)
                                                                                                                                          cAAAAACCCGCCGACGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            <  1:2087667/150‑1 (MQ=38)
                                                                                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt           >  1:2012675/1‑150 (MQ=37)
                                                                                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt           >  2:923897/1‑150 (MQ=37)
                                                                                                                                           aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCCAAAGCAAACACCCGCCGACGCGGGTGTGTACGGAAATCAggg           >  2:2162057/1‑149 (MQ=11)
                                                                                                                                            aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGAGGCCAGTCAATCAACGTTAAGCCCCTCCAAAAGCCAAAACCCGCCGAACCCGGGTGTTACGTAAATGAGTTg          >  2:1434522/1‑150 (MQ=11)
                                                                                                                                             aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa         >  1:310173/1‑150 (MQ=37)
                                                                                                                                                 ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt     >  2:1799526/1‑150 (MQ=37)
                                                                                                                                                 ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt     >  2:612813/1‑150 (MQ=37)
                                                                                                                                                 ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt     >  2:1213357/1‑150 (MQ=37)
                                                                                                                                                     |                                                                                                                                                    
ATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269931‑3270228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: