Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,081 G→T 5.5% intergenic (+479/+135) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,0810GT 5.5% 365.9 / 11.5 127intergenic (+479/+135)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base G (58/60);  new base T (3/4);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 132 total aligned reads displayed.

TTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269932‑3270228
                                                                                                                                                     |                                                                                                                                                   
ttcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                                                                     >  1:671222/1‑150 (MQ=255)
      tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  2:1981806/150‑1 (MQ=37)
      tttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  1:474228/150‑1 (MQ=37)
      tttCTGCCACTCCAAAAGCAAAAACCCGCCGTATCGGGTTTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                                                                               <  2:983777/150‑1 (MQ=11)
          tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:1090426/150‑1 (MQ=37)
          tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  2:657652/150‑1 (MQ=37)
          tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  2:313613/150‑1 (MQ=37)
          tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:2115827/150‑1 (MQ=37)
          tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:1873739/150‑1 (MQ=37)
          tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                                                                           <  1:1387279/150‑1 (MQ=37)
           gCCCCTCCAAAATTAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                                                                          <  1:619358/150‑1 (MQ=11)
           gCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                                                                          <  1:619368/150‑1 (MQ=255)
            ccccTCCACAAGTAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATCAGCCGCTTTCTTTTGGGGATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                                         <  2:2392636/150‑1 (MQ=255)
            ccccTCCACAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                                                                         <  1:1098223/150‑1 (MQ=37)
             cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                                                                        <  1:507159/150‑1 (MQ=37)
             cccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGGCGGGGAAAGTCAGTCAAGGTGGTGCACCGACAAAAGGAAAAACCCGCCGCAGCTGGTTTTTa                                                                                                                                        >  1:175104/1‑150 (MQ=11)
                tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                     >  1:1000685/1‑150 (MQ=37)
                tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                     >  1:2191823/1‑150 (MQ=37)
                tCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                                                                     >  2:169120/1‑150 (MQ=37)
                  cAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGtttttgctttt                                                                                                                                   <  2:609057/150‑7 (MQ=11)
                    aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                 >  1:1255740/1‑150 (MQ=38)
                              cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                                                       <  1:2094497/150‑1 (MQ=37)
                                 gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                                                                    >  1:1983933/1‑150 (MQ=17)
                                 gccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCACCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                                                    <  2:1357485/150‑1 (MQ=37)
                                  ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  1:340165/150‑1 (MQ=37)
                                  ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                   <  1:1301928/150‑1 (MQ=37)
                                   cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATATTTATGCCACTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                                  >  1:1714691/1‑150 (MQ=255)
                                       gCGGTTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  1:1245706/150‑1 (MQ=255)
                                       gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                              <  1:2343298/150‑1 (MQ=37)
                                          ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                           >  2:267874/1‑150 (MQ=17)
                                          ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                           >  2:542317/1‑150 (MQ=17)
                                             ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                        <  2:940547/150‑1 (MQ=38)
                                             ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                        <  2:446859/150‑1 (MQ=38)
                                                 aCGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcgttg                                                                                                    >  1:537867/1‑149 (MQ=255)
                                                    tAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttcttt                                                                                                 >  2:1629184/1‑150 (MQ=40)
                                                      aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                               <  1:1382029/150‑1 (MQ=39)
                                                       aaCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                              <  2:1541446/150‑1 (MQ=255)
                                                          aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGta                                                                                           >  1:1725520/1‑150 (MQ=17)
                                                            gtaaaaCTGACCGATAAGCCGCCTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                         <  1:1925468/147‑1 (MQ=17)
                                                            gTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCACTCCAAAATCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                         <  1:81230/150‑1 (MQ=11)
                                                            gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                         >  2:544021/1‑150 (MQ=37)
                                                            gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGCGTTGTTTGGGGtata                                                                                         >  2:934749/1‑150 (MQ=255)
                                                            gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTggggaga                                                                                         >  2:1411234/1‑146 (MQ=12)
                                                            gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGAACGATAATCAGCTTTCTTTTGGGtata                                                                                         >  2:2017963/1‑150 (MQ=11)
                                                             taaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                        >  2:150019/3‑118 (MQ=18)
                                                             taaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                        <  1:150019/116‑1 (MQ=18)
                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTAATCTTTATGCCCCACCAAAATCAAAAAACCCCCCCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTGTAGcgtcgt                                                                                  >  1:713581/1‑150 (MQ=11)
                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgg                                                                                  >  2:1325788/1‑149 (MQ=37)
                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGCAGCGGGTTTTTTCGTAAACCAGGGGAAACAGACCGAAAAGCCGCGTTAGTTTGGGTATAGcgtcgg                                                                                  >  1:1159022/1‑149 (MQ=11)
                                                                   tGACCGATAAGCCGCTTTCGTTTGGGTATAGTGTCGTGGACAGTCCATCATCGTTCTGCCCCACCCAAAGCAAAAAACCCCCCAAGCCGGTTTTTACCTAAACCCCGTGAAACTGACCCAAAAGCCGCGTTCGTTTGGGGATAGagtcgg                                                                                  >  1:2191325/1‑149 (MQ=2)
                                                                   tGACCGATAAGCCGCTTGCTTTTGGGTTTCGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                  <  1:761767/150‑1 (MQ=255)
                                                                   tGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGGAAACAAGGTGAAACTGACCGATAAGCCCGGTTCTTTTGGGGTGAGcgtcgt                                                                                  >  1:3606/1‑150 (MQ=11)
                                                                    gcccGATAACCAGCTTTCTTGTGGGTATTGCGCCGTGGTCAGCCATTCATCTCTCTGCCCCTCCAAAATCTAAAACCCGCCGCAGCGGGTTTTTACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                 <  2:886163/148‑1 (MQ=11)
                                                                    gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                 <  1:865248/150‑1 (MQ=37)
                                                                    gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                 <  1:2029990/150‑1 (MQ=37)
                                                                    gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGACGTg                                                                                 >  2:388388/1‑150 (MQ=37)
                                                                    gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTg                                                                                 >  2:388405/1‑150 (MQ=255)
                                                                        taTAAGCCGCTTTCTTTTGGGCATAGTGTCGCGGACAGTCACTCTTATTTATGCCCCTCCACAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                             <  2:1199242/149‑1 (MQ=11)
                                                                           aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                                                          <  1:2310346/150‑1 (MQ=37)
                                                                              ctgCTTTCTTTTTTGTTCTGTGTCGGGGACTGTGTATATTCTTTATGGCCCTCCAAAAGCTTAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  1:1227762/148‑1 (MQ=2)
                                                                              ccGCATTCTTTTTGGAATATTGACGTGGACAGTCTTTCATCCTGCTGCCCCTCCAGATGATTAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                       <  2:1926579/150‑1 (MQ=11)
                                                                                        ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTg                                                             >  2:1308815/1‑150 (MQ=255)
                                                                                        ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCAAGGCATTCATCTTTCtc                                                             >  2:1308574/1‑149 (MQ=11)
                                                                                         tGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTGTGGGTAGAGCGTCGTGGCCAGGCCTTCATCGTTCTGc                                                            >  2:1728246/1‑150 (MQ=11)
                                                                                             tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATATTTCAGcccca                                                        >  2:534607/1‑149 (MQ=255)
                                                                                                      gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCAATCATCATTCTGCCCCTCCAAAATCa                                               >  2:1618889/1‑150 (MQ=11)
                                                                                                      gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATAGTTATGCCCCACCAAAAGCa                                               >  2:1447041/1‑150 (MQ=11)
                                                                                                            aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAAATAATGCCCCACCAAAAGCAAAAAcc                                         >  1:153719/1‑150 (MQ=11)
                                                                                                            aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGTGTATAGCGTAGTGGCAAGTCATTAATATTCCAGCACCACCAAAATCAAAAAcc                                         >  1:309105/1‑150 (MQ=11)
                                                                                                              tctttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                       <  2:2191823/147‑1 (MQ=37)
                                                                                                              tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                               <  1:1560344/94‑1 (MQ=37)
                                                                                                              tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                       <  1:169120/150‑1 (MQ=37)
                                                                                                              tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                       <  2:1000685/150‑1 (MQ=37)
                                                                                                                attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                     >  1:2137035/1‑150 (MQ=37)
                                                                                                                attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                     >  1:1401532/1‑150 (MQ=37)
                                                                                                                     tCTTTCTGCCCCTCCAACAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAGc                                <  1:1831900/150‑1 (MQ=255)
                                                                                                                           tGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                          <  2:1776816/150‑1 (MQ=255)
                                                                                                                           tGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAATCCCGCCGAAGCGGGttt                          <  2:2260783/150‑1 (MQ=255)
                                                                                                                            gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  1:1436324/1‑150 (MQ=37)
                                                                                                                            gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  2:2101577/1‑150 (MQ=37)
                                                                                                                            gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                         >  1:384237/1‑150 (MQ=37)
                                                                                                                            gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTAGGCCCCACCAAAAGCAAAAACCCCCCGAAGCGGGtttt                         >  2:1652711/1‑150 (MQ=11)
                                                                                                                            gCCCCGCCATCAGCTAAAACCCGCCGCAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                         <  1:1754039/150‑1 (MQ=11)
                                                                                                                              cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGGCCTTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTGTTa                       >  2:1722843/1‑150 (MQ=11)
                                                                                                                               ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                      <  1:284838/150‑1 (MQ=37)
                                                                                                                               ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                      <  1:489067/150‑1 (MQ=37)
                                                                                                                                 tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                    >  2:1072067/1‑150 (MQ=37)
                                                                                                                                    aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGGTTACGTcaa                 >  2:737846/1‑150 (MQ=255)
                                                                                                                                         taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            >  2:1610356/2‑150 (MQ=255)
                                                                                                                                         taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAAACCCCCGAAGCGGGGTTTTACGTAAATCAgg            >  2:1038988/2‑150 (MQ=11)
                                                                                                                                         taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCCgg            >  1:1361088/2‑150 (MQ=11)
                                                                                                                                         ccgccccccGCCGGAGCGGGTTTGTACGTACCCCCGGTGACACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            <  2:2187261/144‑1 (MQ=11)
                                                                                                                                         cacaaaCCCGCCGTAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            <  1:2241420/147‑1 (MQ=255)
                                                                                                                                         cacaaaCCCGCCGCATCGGGTTATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            <  2:1371107/147‑1 (MQ=11)
                                                                                                                                         cAAAAACCCGCCGACGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg            <  1:2087667/150‑1 (MQ=38)
                                                                                                                                            aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa         >  1:310173/1‑150 (MQ=37)
                                                                                                                                                ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt     >  2:612813/1‑150 (MQ=37)
                                                                                                                                                ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt     >  2:1799526/1‑150 (MQ=37)
                                                                                                                                                ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt     >  2:1213357/1‑150 (MQ=37)
                                                                                                                                                   ccgAAGCGGGTTTTTACGTAACCCAGGTGACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc  <  2:714383/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAC  >  NC_000913/3269932‑3270228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: