Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 5.1% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 5.1% 327.2 / 2.2 121intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (57/53);  new base T (2/4);  total (61/60)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGC  >  NC_000913/3269999‑3270287
                                                                                                                                                |                                                                                                                                                
tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTAATCTTTATGCCCCACCAAAATCAAAAAACCCCCCCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTGTAGcgtcgt                                                                                                                                             >  1:713581/1‑150 (MQ=11)
tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgg                                                                                                                                             >  2:1325788/1‑149 (MQ=37)
tGACCGATAAGCCGCTTTCGTTTGGGTATAGTGTCGTGGACAGTCCATCATCGTTCTGCCCCACCCAAAGCAAAAAACCCCCCAAGCCGGTTTTTACCTAAACCCCGTGAAACTGACCCAAAAGCCGCGTTCGTTTGGGGATAGagtcgg                                                                                                                                             >  1:2191325/1‑149 (MQ=2)
tGACCGATAAGCCGCTTGCTTTTGGGTTTCGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                             <  1:761767/150‑1 (MQ=255)
tGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGGAAACAAGGTGAAACTGACCGATAAGCCCGGTTCTTTTGGGGTGAGcgtcgt                                                                                                                                             >  1:3606/1‑150 (MQ=11)
 gcccGATAACCAGCTTTCTTGTGGGTATTGCGCCGTGGTCAGCCATTCATCTCTCTGCCCCTCCAAAATCTAAAACCCGCCGCAGCGGGTTTTTACGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                                                                            <  2:886163/148‑1 (MQ=11)
 gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGACGTg                                                                                                                                            >  2:388388/1‑150 (MQ=37)
 gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTg                                                                                                                                            >  2:388405/1‑150 (MQ=255)
     taTAAGCCGCTTTCTTTTGGGCATAGTGTCGCGGACAGTCACTCTTATTTATGCCCCTCCACAAGCAAAAACCCGCCGAATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                        <  2:1199242/149‑1 (MQ=11)
        aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtc                                                                                                                                     <  1:2310346/150‑1 (MQ=37)
           ctgCTTTCTTTTTTGTTCTGTGTCGGGGACTGTGTATATTCTTTATGGCCCTCCAAAAGCTTAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                  <  1:1227762/148‑1 (MQ=2)
           ccGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                  <  1:1227737/150‑1 (MQ=37)
           ccGCATTCTTTTTGGAATATTGACGTGGACAGTCTTTCATCCTGCTGCCCCTCCAGATGATTAAACCCGCCGTATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcatt                                                                                                                                  <  2:1926579/150‑1 (MQ=11)
                     ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTTTCTg                                                                                                                        >  2:1308815/1‑150 (MQ=255)
                     ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGCAAGGCATTCATCTTTCtc                                                                                                                        >  2:1308574/1‑149 (MQ=11)
                      tGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTGTGGGTAGAGCGTCGTGGCCAGGCCTTCATCGTTCTGc                                                                                                                       >  2:1728246/1‑150 (MQ=11)
                          tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATATTTCAGcccca                                                                                                                   >  2:534607/1‑149 (MQ=255)
                                   gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCAATCATCATTCTGCCCCTCCAAAATCa                                                                                                          >  2:1618889/1‑150 (MQ=11)
                                   gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATAGTTATGCCCCACCAAAAGCa                                                                                                          >  2:1447041/1‑150 (MQ=11)
                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAAATAATGCCCCACCAAAAGCAAAAAcc                                                                                                    >  1:153719/1‑150 (MQ=11)
                                         aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGTGTATAGCGTAGTGGCAAGTCATTAATATTCCAGCACCACCAAAATCAAAAAcc                                                                                                    >  1:309105/1‑150 (MQ=11)
                                           tctttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                  <  2:2191823/147‑1 (MQ=37)
                                           tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                  <  2:1000685/150‑1 (MQ=37)
                                             attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                                                                >  1:1401532/1‑150 (MQ=37)
                                                cttCTTTCTGCCACTCCAACATCAAAAACCCGCCGAAGCGGTTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                                                             <  2:1333812/148‑1 (MQ=11)
                                                  tCTTTCTGCCCCTCCAACAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGAAGc                                                                                           <  1:1831900/150‑1 (MQ=255)
                                                        tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                     <  2:568586/150‑1 (MQ=37)
                                                        ggCCCCTCCAAAAGCACAAACCCGCCGTAGCGGTTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                     <  2:2260892/149‑1 (MQ=255)
                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                    >  1:1436324/1‑150 (MQ=37)
                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                    >  1:384237/1‑150 (MQ=37)
                                                         gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                    >  2:2101577/1‑150 (MQ=37)
                                                         gCCCCGCCATCAGCTAAAACCCGCCGCAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt                                                                                    <  1:1754039/150‑1 (MQ=11)
                                                          ccacTCCAAAAGCAAAAACCCGCCGAATCTGGTTTTTACGTAAACCATGTGAATCTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTTTACAGTAATTAATCTTTCTGCCCCTCCAAAATAAAAAACCCGCTTCGGCGGGttttt                                                                                   <  1:451620/147‑1 (MQ=11)
                                                           cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGGCCTTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTGTTa                                                                                  >  2:1722843/1‑150 (MQ=11)
                                                            ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                 <  1:284838/150‑1 (MQ=37)
                                                            ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                 <  1:489067/150‑1 (MQ=37)
                                                              tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                               >  2:1072067/1‑150 (MQ=37)
                                                                 aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGGTTACGTcaa                                                                            >  2:737846/1‑150 (MQ=255)
                                                                      taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                       >  2:1610356/2‑150 (MQ=255)
                                                                      taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCCgg                                                                       >  1:1361088/2‑150 (MQ=11)
                                                                      ccgccccccGCCGGAGCGGGTTTGTACGTACCCCCGGTGACACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                       <  2:2187261/144‑1 (MQ=11)
                                                                      cacaaaCCCGCCGTAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                       <  1:2241420/147‑1 (MQ=255)
                                                                      cacaaaCCCGCCGCATCGGGTTATTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                       <  2:1371107/147‑1 (MQ=11)
                                                                      cAAAAACCCGCCGACGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                       <  1:2087667/150‑1 (MQ=38)
                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                      >  2:923897/1‑150 (MQ=37)
                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                      >  1:2012675/1‑150 (MQ=37)
                                                                       aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTGTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCCAAAGCAAACACCCGCCGACGCGGGTGTGTACGGAAATCAggg                                                                      >  2:2162057/1‑149 (MQ=11)
                                                                        aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGAGGCCAGTCAATCAACGTTAAGCCCCTCCAAAAGCCAAAACCCGCCGAACCCGGGTGTTACGTAAATGAGTTg                                                                     >  2:1434522/1‑150 (MQ=11)
                                                                             ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                >  2:1799526/1‑150 (MQ=37)
                                                                             ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                >  2:1213357/1‑150 (MQ=37)
                                                                                ccgAAGCGGGTTTTTACGTAACCCAGGTGACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc                                                             <  2:714383/150‑1 (MQ=255)
                                                                                      cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                       >  1:1625469/1‑150 (MQ=37)
                                                                                           ttttACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                  <  2:572948/150‑1 (MQ=2)
                                                                                           ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                  <  1:267874/150‑1 (MQ=38)
                                                                                           ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                  <  1:1610356/150‑1 (MQ=38)
                                                                                           ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                  <  1:1585216/150‑1 (MQ=38)
                                                                                           ttttACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                  <  1:542317/150‑1 (MQ=17)
                                                                                           tattACGTACACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                  <  2:1276769/148‑1 (MQ=255)
                                                                                            tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCggg                                                 >  2:975657/1‑150 (MQ=38)
                                                                                             ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAATGACCGATAAGCCGGGt                                                >  1:1022300/1‑150 (MQ=255)
                                                                                             ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGAACGATAATCCGGGt                                                >  1:1488612/1‑150 (MQ=255)
                                                                                             ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGt                                                >  1:1032256/1‑150 (MQ=255)
                                                                                                cGTACACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCt                                             <  1:382391/150‑1 (MQ=255)
                                                                                                cGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAGAGTGATTGATGTTTCTGCGCCTCCAAATGCAAATACCCGGCGGAGGGGGTTTTTTCGTAATTGAGGTGTATCTGTGCGATATGGGGGGttgt                                             >  2:2351289/1‑148 (MQ=2)
                                                                                                 gtacatcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGt                                           <  2:710051/144‑1 (MQ=11)
                                                                                                 gTAAACCAGGTGCAACTGACCGCTGATCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTg                                            <  2:1869632/150‑1 (MQ=14)
                                                                                                 gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTg                                            >  1:718372/1‑150 (MQ=255)
                                                                                                     atcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                        <  1:775972/148‑1 (MQ=255)
                                                                                                     aactggTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                        <  1:1252528/146‑1 (MQ=255)
                                                                                                     aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                        <  1:542930/150‑1 (MQ=255)
                                                                                                       cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                      >  2:442623/1‑150 (MQ=255)
                                                                                                        aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACTGACCGATAAGCCGGGTTgtgtggggta                                     >  1:1233859/1‑144 (MQ=11)
                                                                                                          gtaaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTTCCCCTCCAAATGAAAAAAACCGGCGAAGCGGGTTTTTTAGTAAATATTGTGTAAGTGACCGATAAGGCGGGTTGTGTTGTGGata                                   >  2:1685575/4‑148 (MQ=11)
                                                                                                          gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                   <  2:310173/150‑1 (MQ=255)
                                                                                                             aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCCGTCATTGATCTTTCTGCCCCGCCAAAATCAAAAAACCCCCGAAGCGGGTTTTTTCGGAAATCAGGTGTAACAGACCGATAAGCCGGGGTCTGTCGGGGGCCGtc                                >  1:1061361/1‑150 (MQ=14)
                                                                                                              caCTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAACTCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtca                               <  2:2191325/149‑1 (MQ=255)
                                                                                                                cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcatt                             <  1:996406/150‑1 (MQ=255)
                                                                                                                cTGACATATAAGCCGATTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCACCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcatt                             <  2:1714691/150‑1 (MQ=255)
                                                                                                                 tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTAATTCATAATTATGCCCCGCCAAAAACAAAAACCCCCCCAAGCGGGTTTTTACCTAAATAAAGTGGAACAGACCGATAAGCCGGGTTATTTCGTGGGCCGtcattg                            >  1:2218371/1‑149 (MQ=14)
                                                                                                                 tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                            >  2:1781119/1‑150 (MQ=255)
                                                                                                                 tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                            >  2:573001/1‑150 (MQ=255)
                                                                                                                 tGACCGATAAGCCGCTTTCTTTTGGGTATAGCCTCGTGGACAGTCAATAAACATTATTCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTAAGAAAATAAGGTGAAACAGACCCCTAAGCCCGGTTATGTCGTGGTAAGtaattc                            >  1:263386/1‑150 (MQ=9)
                                                                                                                 cgACCGAAAAGCCGCTTTCTTTTGGGTATAGCGTCGTTCACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                            <  1:1685575/149‑1 (MQ=255)
                                                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTg                                                                                                                        >  2:75618/1‑56 (MQ=35)
                                                                                                                   aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTg                                                                                                                        <  1:75618/56‑1 (MQ=35)
                                                                                                                      gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                       <  1:1072067/150‑1 (MQ=255)
                                                                                                                      gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                       <  1:923897/150‑1 (MQ=255)
                                                                                                                       aTAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                      <  1:749158/150‑1 (MQ=255)
                                                                                                                            ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCttt                                                                                                                           <  1:71376/44‑1 (MQ=38)
                                                                                                                            ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATATAGGCCAg                 >  2:2046308/1‑150 (MQ=255)
                                                                                                                             cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                   <  1:604670/83‑1 (MQ=33)
                                                                                                                             cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGatttt                                                                                   <  1:1487858/83‑1 (MQ=18)
                                                                                                                             cGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                <  1:2039189/150‑1 (MQ=255)
                                                                                                                              gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa               >  2:1543193/1‑150 (MQ=255)
                                                                                                                                  tctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg           <  1:2038429/150‑1 (MQ=255)
                                                                                                                                  tctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg           <  1:1864747/150‑1 (MQ=255)
                                                                                                                                      ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa       <  2:313225/150‑1 (MQ=255)
                                                                                                                                      ttGGGTATAGCGTCGTGGTCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa       <  1:1434522/150‑1 (MQ=255)
                                                                                                                                      ttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa       >  2:2097273/1‑150 (MQ=255)
                                                                                                                                           tataGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGTCCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATAGCTCACTGGc  >  2:1430/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGC  >  NC_000913/3269999‑3270287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: