Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,283,652 C→T 5.7% D170D (GAC→GAT kbaY → tagatose 6‑phosphate aldolase 1, kbaY subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,283,6520CT 5.7% 488.6 / 11.1 141D170D (GAC→GATkbaYtagatose 6‑phosphate aldolase 1, kbaY subunit
Reads supporting (aligned to +/- strand):  ref base C (53/80);  new base T (4/4);  total (57/84)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 142 total aligned reads displayed.

TTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCC  >  NC_000913/3283504‑3283797
                                                                                                                                                    |                                                                                                                                                 
ttgACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACa                                                                                                                                                  <  1:1230458/150‑1 (MQ=255)
  gACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGc                                                                                                                                                >  1:1900333/1‑150 (MQ=255)
   aCTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTTGCGTCGACAGcc                                                                                                                                               <  2:220985/150‑1 (MQ=255)
   aCTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGcc                                                                                                                                               <  2:1865235/150‑1 (MQ=255)
     ttCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTg                                                                                                                                             <  1:1131993/150‑1 (MQ=255)
     ttCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTg                                                                                                                                             <  2:35942/150‑1 (MQ=255)
       cTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGc                                                                                                                                           <  2:1814225/150‑1 (MQ=255)
       cTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGc                                                                                                                                           <  1:197438/150‑1 (MQ=255)
        tGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCg                                                                                                                                          >  1:2237520/1‑150 (MQ=255)
        tGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCg                                                                                                                                          >  2:2265802/1‑150 (MQ=255)
          ccACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGt                                                                                                                                        >  1:1682157/1‑150 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACTAGAAGCTAAACGCTTTGTCGAACTGACTGGCGACGACAGCCTGGCGGTAGCGa                                                                                                                                   >  2:2219616/1‑150 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGa                                                                                                                                   >  2:492202/1‑150 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGa                                                                                                                                   >  2:793578/1‑150 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGa                                                                                                                                   >  2:793645/1‑150 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGa                                                                                                                                   >  2:492245/1‑150 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGa                                                                                                                                   <  2:2191723/150‑1 (MQ=255)
               cacaAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGa                                                                                                                                   >  1:883089/1‑150 (MQ=255)
                 caAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGAtt                                                                                                                                 <  1:1862294/150‑1 (MQ=255)
                  aaGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTg                                                                                                                                <  2:1984382/150‑1 (MQ=255)
                  aaGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTg                                                                                                                                <  2:2182806/150‑1 (MQ=255)
                  aaGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTg                                                                                                                                <  2:1984409/150‑1 (MQ=255)
                  aaGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTg                                                                                                                                <  2:1995940/150‑1 (MQ=255)
                  aaGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTg                                                                                                                                >  1:237766/1‑150 (MQ=255)
                        gCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGGGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACgg                                                                                                                          <  1:2344857/150‑1 (MQ=255)
                           gCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGgcgc                                                                                                                       >  1:2043204/1‑150 (MQ=255)
                            cGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCa                                                                                                                      >  2:2390841/1‑150 (MQ=255)
                            cGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCa                                                                                                                      <  2:1198369/150‑1 (MQ=255)
                            cGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTATGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCa                                                                                                                      >  2:2390811/1‑150 (MQ=255)
                             gTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCAc                                                                                                                     <  2:428280/150‑1 (MQ=255)
                                gAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGc                                                                                                                  >  2:1630687/1‑150 (MQ=255)
                                gAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGATAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGc                                                                                                                  >  2:950507/1‑150 (MQ=255)
                                 aaGCAGAACTGGGTCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCt                                                                                                                 >  1:1951975/1‑150 (MQ=255)
                                 aaGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCt                                                                                                                 <  1:480551/150‑1 (MQ=255)
                                     aGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTtata                                                                                                             >  1:271039/1‑150 (MQ=255)
                                       aaCTGGGTCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACa                                                                                                           >  2:338788/1‑150 (MQ=255)
                                       aaCTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACa                                                                                                           >  1:1947191/1‑150 (MQ=255)
                                       aaCTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACa                                                                                                           >  1:1382300/1‑150 (MQ=255)
                                       aaCTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACa                                                                                                           >  1:1382322/1‑150 (MQ=255)
                                       aaCTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACa                                                                                                           <  1:2296335/150‑1 (MQ=255)
                                       aaCTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACa                                                                                                           >  2:694015/1‑150 (MQ=255)
                                              ccgccTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAAc                                                                                                    >  2:413744/1‑150 (MQ=255)
                                               cgccTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACg                                                                                                   >  1:646330/1‑150 (MQ=255)
                                                gccTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGc                                                                                                  <  2:356815/150‑1 (MQ=255)
                                                   tGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGa                                                                                               >  2:1263751/1‑150 (MQ=255)
                                                    gggCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGaa                                                                                              <  1:1222496/150‑1 (MQ=255)
                                                       cGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAgat                                                                                           <  2:526346/150‑1 (MQ=255)
                                                        ggTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGGGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAgatt                                                                                          <  1:752171/150‑1 (MQ=255)
                                                          tgtTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAgattga                                                                                        <  2:173383/150‑1 (MQ=255)
                                                              gAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTc                                                                                    <  2:134143/150‑1 (MQ=255)
                                                                  aTGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGc                                                                                >  1:1168170/1‑150 (MQ=255)
                                                                  aTGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGc                                                                                >  1:314826/1‑150 (MQ=255)
                                                                     aCATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGc                                                                             >  2:904605/1‑150 (MQ=255)
                                                                     aCATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGc                                                                             <  1:2290593/150‑1 (MQ=255)
                                                                        tGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTgg                                                                          <  1:1606933/150‑1 (MQ=255)
                                                                          aGCGTTGACGCCTAAAGTGAATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCg                                                                        <  1:984245/150‑1 (MQ=255)
                                                                          aGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCg                                                                        <  1:1125932/150‑1 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTTAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    <  1:1160689/150‑1 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    <  1:518077/150‑1 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    <  1:518075/150‑1 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    <  1:518049/150‑1 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    >  2:2259745/1‑150 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    >  2:580255/1‑150 (MQ=255)
                                                                                              aTTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGa                                                    <  1:1359616/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCTAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:868789/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:1079638/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:2346112/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:2271892/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:963466/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:905968/150‑1 (MQ=255)
                                                                                                 ccTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGt                                                 <  2:1079671/150‑1 (MQ=255)
                                                                                                     aCCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCct                                             >  1:1525889/1‑150 (MQ=255)
                                                                                                       cGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCctct                                           <  1:1193729/150‑1 (MQ=255)
                                                                                                       cGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCctct                                           <  2:1739216/150‑1 (MQ=255)
                                                                                                       cGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCctct                                           <  2:1739185/150‑1 (MQ=255)
                                                                                                       cGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCctct                                           >  1:421178/1‑150 (MQ=255)
                                                                                                        gATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTg                                          >  1:2053297/1‑150 (MQ=255)
                                                                                                         aTCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTgg                                         >  1:1700582/1‑150 (MQ=255)
                                                                                                         aTCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTgg                                         >  1:2114035/1‑150 (MQ=255)
                                                                                                          tCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGt                                        <  1:1130804/150‑1 (MQ=255)
                                                                                                            cacaAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGtgc                                      >  2:1444871/1‑150 (MQ=255)
                                                                                                             acaAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGtgct                                     >  2:1431003/1‑150 (MQ=255)
                                                                                                              caAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGtgctg                                    >  1:2355375/1‑150 (MQ=255)
                                                                                                                 gaagCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCAt                                 >  2:490373/1‑150 (MQ=255)
                                                                                                                  aagCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATg                                <  2:656879/150‑1 (MQ=255)
                                                                                                                    gCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGt                              <  1:1417691/150‑1 (MQ=255)
                                                                                                                        aaCGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCa                          >  1:2280805/1‑150 (MQ=255)
                                                                                                                         aCGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAg                         <  1:793645/150‑1 (MQ=255)
                                                                                                                         aCGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAg                         <  1:793578/150‑1 (MQ=255)
                                                                                                                         aCGCTTTGTCGAACTGACTGGCGTCGATAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAg                         <  1:492202/150‑1 (MQ=255)
                                                                                                                                   gAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCg               <  2:2237520/150‑1 (MQ=255)
                                                                                                                                   gAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCg               <  1:2265802/150‑1 (MQ=255)
                                                                                                                                    aaCTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATTTTCCgg              <  2:1539102/150‑1 (MQ=255)
                                                                                                                                     actgactgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGa             <  1:250176/150‑1 (MQ=255)
                                                                                                                                          ctgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAAt        <  1:1598979/150‑1 (MQ=255)
                                                                                                                                          ctgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAAt        <  1:832584/150‑1 (MQ=255)
                                                                                                                                          ctgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAAt        <  1:1295398/150‑1 (MQ=255)
                                                                                                                                          ctgGCGGCGACAGCCAGGCGGTAGCGATCGGCACGGCGCACGGCTTATACAGCAAAACGCCGAAGAATGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAAt        <  1:1484630/150‑1 (MQ=255)
                                                                                                                                             gCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTg     >  1:2319033/1‑150 (MQ=255)
                                                                                                                                             gCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTg     >  1:1813897/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTGACTTCTGCCACTCACAAGATTGCAGCGTGGAAGCAGAACTGGGCCGCCTGGGCGGTGTTGAAGATGACATGAGCGTTGACGCCGAAAGTGCATTCCTGACCGATCCACAAGAAGCTAAACGCTTTGTCGAACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCC  >  NC_000913/3283504‑3283797

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: