Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,189 T→G 5.2% intergenic (‑161/+269) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,1890TG 5.2% 544.6 / 16.0 188intergenic (‑161/+269)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base T (88/88);  new base G (7/4);  total (96/92)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAT  >  NC_000913/3392042‑3392337
                                                                                                                                                   |                                                                                                                                                     
cGTTAGTTACTGCCGTAGAGGATTTTTTACGGCTGACGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGCTAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTa                                                                                                                                                     >  1:742206/1‑150 (MQ=255)
  ttagttaCTGCAGTAGAGTATTTTTTCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                   <  2:1436568/150‑1 (MQ=255)
  ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                   >  1:1217233/1‑150 (MQ=255)
  ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                   >  1:1889176/1‑150 (MQ=255)
     gttaCTGCAGTCGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGa                                                                                                                                                <  2:1126984/150‑1 (MQ=255)
      ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGTTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                                               <  2:2125018/150‑1 (MQ=255)
      ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                                               <  2:277043/150‑1 (MQ=255)
      ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                               <  2:1683179/150‑1 (MQ=18)
       taCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATAAGGCGTTTACGCCGCATCCGAca                                                                                                                                              <  2:449877/150‑2 (MQ=18)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAATACGAACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGAAAACGCCGTATCCGACCt                                                                                                                                             >  2:970399/1‑150 (MQ=18)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                             >  1:1249478/1‑150 (MQ=255)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                             >  2:2015897/1‑150 (MQ=255)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                             >  1:1288502/1‑150 (MQ=255)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGAGGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                             >  1:2172257/1‑150 (MQ=255)
            cAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                         >  1:722005/1‑150 (MQ=255)
             cggagagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTGGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                        <  2:1722797/147‑1 (MQ=255)
                agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                                                                     <  2:123811/150‑1 (MQ=255)
                    gATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTAGCATCAGTCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAGGTTc                                                                                                                                 >  2:2302272/1‑150 (MQ=255)
                      ttttttCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCATACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCg                                                                                                                               <  2:859034/150‑1 (MQ=255)
                       tttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGt                                                                                                                             >  1:1278043/1‑150 (MQ=255)
                         tttCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                                           <  2:296810/150‑1 (MQ=255)
                         tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                                           <  2:427046/150‑1 (MQ=255)
                            ccggcggCCGGTGGCGGCGTGAACGCCCTATCCAGCCTACGGTTTTGTTCGGTTTTGTCGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                                                        <  1:2351204/149‑1 (MQ=14)
                                cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                                                                                    >  2:349083/1‑150 (MQ=255)
                                 tGCCGGATGGGGCTTGACCGCCCTACCCACCATACGTTTTTGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                   <  1:1036752/150‑1 (MQ=14)
                                 tGCCGGATGCGGCGTTACCGCCCTACCCAGCCTACGTTTATGTTCCGGTTTGTTGGCCTTATAAGACGCACTGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                   <  1:2335898/150‑1 (MQ=14)
                                 tGCCGGATGCGGCGTTAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                   <  1:250280/150‑1 (MQ=255)
                                 tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCGGGTTTGTAGGCCGGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                   <  2:824562/150‑1 (MQ=255)
                                 tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                   <  2:1279750/150‑1 (MQ=255)
                                 tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGAACAGCGACGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTGATGTTCCGTTTGTAGGCCt                                                                                                                   >  2:1048038/1‑150 (MQ=255)
                                 tGCCGGAGGCGGCGTGAACGCCCCACCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCCTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                   <  1:1150796/150‑1 (MQ=17)
                                     ggATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                                                                                                               <  2:1646859/150‑1 (MQ=255)
                                          cggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                                                                                                          <  2:2169902/150‑1 (MQ=255)
                                           ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                                                                                                         >  1:1329102/1‑150 (MQ=255)
                                            gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                                                                        <  1:349053/150‑1 (MQ=255)
                                            gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                                                                        <  1:349093/150‑1 (MQ=255)
                                             cgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGcac                                                                                                       >  1:919759/1‑150 (MQ=255)
                                                      ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATCAGACGCACAGCGTCGCa                                                                                              >  2:1610258/1‑150 (MQ=255)
                                                      ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                              >  2:1049656/1‑150 (MQ=255)
                                                       cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                             >  1:615436/1‑150 (MQ=255)
                                                       cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                             >  1:1764090/1‑150 (MQ=255)
                                                        tATCCAGCCTACGGTTATGTTCGGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                                            <  2:2089343/150‑1 (MQ=255)
                                                        tATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                                            >  1:252434/1‑150 (MQ=255)
                                                        tATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATc                                                                                            <  2:2090831/150‑1 (MQ=255)
                                                         acccACCCTAGGGTTATGGTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAACAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                           <  1:1397870/148‑1 (MQ=255)
                                                         aTCCAGCCTCCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                           <  1:765925/150‑1 (MQ=255)
                                                         aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                           <  1:318193/150‑1 (MQ=255)
                                                         aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                           <  1:1834319/150‑1 (MQ=255)
                                                                 caCGGTTAGGTTCCGGCTGGTAGGCCTGATAAGCCGCACCGCGTCGCATCTGGAAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACgg                                                                                   <  2:220038/149‑1 (MQ=11)
                                                                    ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                >  2:84203/1‑150 (MQ=37)
                                                                    ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                >  1:1495201/1‑150 (MQ=37)
                                                                    ggTTATGTTCCGGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGATCCGGGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTg                                                                                >  2:2374669/1‑150 (MQ=11)
                                                                           ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                         >  1:1000698/1‑150 (MQ=35)
                                                                           ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                         >  1:1000569/1‑150 (MQ=35)
                                                                            tCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTATGCCTGATAAGACGCACAGCGTCGCAGCAGGCAACGGCTGCCGGATgc                                                                        >  2:1073600/1‑150 (MQ=255)
                                                                              cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                                      <  2:1773126/150‑1 (MQ=33)
                                                                              cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                                      >  1:1165594/1‑150 (MQ=18)
                                                                              cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGATGTTGGCCTGTTAAGACGCACAGCGAAGCTTCAGGCCACGCCTGACGCAAgcgg                                                                      >  1:2168731/1‑150 (MQ=11)
                                                                               ggTTTGTGGGCCTGATACGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                     <  2:1156137/150‑1 (MQ=255)
                                                                               ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCCTCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGTTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                     <  2:1583552/150‑1 (MQ=255)
                                                                                gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                                                    >  1:1585124/1‑150 (MQ=32)
                                                                                gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                                                    >  1:1642347/1‑150 (MQ=17)
                                                                                       ggCCTGATACGCCGCACAGCGGCGCATCTGGCAACGGCGGGCGGAGGCGGCGTAAACGCCTTAGCCGACCTACGGTTATTTACCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                             <  2:1538305/150‑1 (MQ=11)
                                                                                       ggCCTGATAAGCCGCACCGGGGCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                             <  2:722005/150‑1 (MQ=11)
                                                                                       ggCCTGATAAGACGCACCGCGTCGCATACGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTACCCGACCTCCGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                             <  2:1848056/150‑1 (MQ=11)
                                                                                       ggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                             <  2:2093083/150‑1 (MQ=34)
                                                                                                ctccGCACTGCGGCGCAACGGGAAACGCCTGTCGTATGCGGCTTAAACGCCTTACCCGACATCCGTTTATGTTCCTTTTGTAGGCCTGATAAGACGCACCGCTTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                    <  2:617486/147‑1 (MQ=11)
                                                                                                aGACGCTCAGGTGCGCATCTGGCAACGGGTGTGGTATGCGGCGTAAACGCCTTATCCGACATAGGTTTATGTTCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                    <  2:93108/150‑1 (MQ=11)
                                                                                                aGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                    <  2:2382628/150‑1 (MQ=37)
                                                                                                aGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                    <  2:2401324/150‑1 (MQ=37)
                                                                                                aGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                    >  1:899133/1‑150 (MQ=37)
                                                                                                aGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                    >  1:2218557/1‑150 (MQ=37)
                                                                                                 gACGCACAGCGTCGCATCAGGCAACGGCGGTCGGATGCGGCGTAAACGCCTTATCCGACCTAGGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                                                   <  2:1978626/150‑1 (MQ=255)
                                                                                                  aCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAcc                                                  >  2:569688/1‑150 (MQ=37)
                                                                                                           gTCGCATCTGGCAAGGGGTGTGGGATGCGGCTTAAACGCATTACCCTCCATCGGGTTTTGTACCGTTTTTTGGCCTGATAAGCCGCACAGCGTCGAATATGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                         <  1:1417139/150‑1 (MQ=2)
                                                                                                           gTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                         <  2:137471/150‑1 (MQ=255)
                                                                                                           gTCGCATCGGGAACGGGCTGGCGGTGGCGGCTTAACCGCATTATCCGACCTCGGTTTTTGTTCGTTTTGTTGGCCTTATAAGACCCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                         <  1:544855/150‑1 (MQ=2)
                                                                                                                ttctgccAAGGGCTGTCGGTTGCGGCTTAAACGCCTTATCCTACCTAGGTTTTTGTCCCTTTTTTCGGCCTTATAAGACGCACCGCGTCGAATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                    <  1:463166/144‑1 (MQ=11)
                                                                                                                cccAGGCAACGGCTGCCGGAGGCGGCGTAAACGCCTCATCCGCCCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAACAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                    <  2:559071/148‑1 (MQ=11)
                                                                                                                aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                    >  1:657465/1‑150 (MQ=38)
                                                                                                                aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCAACGGGTATGTTcc                                    >  1:1925246/1‑150 (MQ=255)
                                                                                                                   aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGGTCCGtg                                 >  1:1318988/1‑149 (MQ=255)
                                                                                                                          ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGc                          >  1:1584374/1‑150 (MQ=255)
                                                                                                                          ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                               <  2:1584385/81‑1 (MQ=33)
                                                                                                                           gCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                         <  2:1733898/150‑1 (MQ=255)
                                                                                                                             tGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTg                       >  1:1380817/1‑150 (MQ=255)
                                                                                                                              gccGGAGGCGGCGTAAACGCCTTACCCGACCTCCGGTTATGGACCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                      <  2:332182/148‑1 (MQ=14)
                                                                                                                              gTCGGATGCGGCGAAACCGCCTTATCCGCCCTCGGGTTATGTTCGTTTTGTTGGCCTTATACCACCCACTGGGTCGCATCCGGCCACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                      <  1:218511/150‑1 (MQ=14)
                                                                                                                               ggggttGCGGCGTAACCGCCTTATCCGCCGTTCGGTTATGTTGCTTTTTTCGGCCTGTTACGACGCCGAGCGGCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                     <  2:411913/145‑1 (MQ=14)
                                                                                                                                cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGATa                    >  1:1205927/1‑150 (MQ=255)
                                                                                                                                cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATa                    >  1:2057607/1‑150 (MQ=255)
                                                                                                                                  gATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTGATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAg                  >  1:2288848/1‑150 (MQ=255)
                                                                                                                                   aTGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGa                 <  2:1363328/150‑1 (MQ=255)
                                                                                                                                    taaggcgTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                >  2:1662883/4‑150 (MQ=14)
                                                                                                                                    taaggcgTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                >  2:1683388/4‑150 (MQ=11)
                                                                                                                                     gcggcgTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg               <  2:1329102/150‑1 (MQ=255)
                                                                                                                                                gCCTTATCCGACCTCGGTTTATTTCCGTTTTGTCGGCCTGATAAGACGCACAGCGTCGCTTCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc    <  1:2196376/150‑1 (MQ=11)
                                                                                                                                                cccTTATCCTACCTTCGTTTTTGTTCCTTTTGTTGGCCTGATAAGACGCCCCGCGTCGAATCAGCCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc    <  1:416920/149‑1 (MQ=14)
                                                                                                                                                 ccTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa   <  2:1413062/150‑1 (MQ=255)
                                                                                                                                                  cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt  >  1:1472627/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAT  >  NC_000913/3392042‑3392337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: