Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,392,190 T→C 5.4% intergenic (‑162/+268) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,392,1900TC 5.4% 542.7 / 15.6 188intergenic (‑162/+268)tldD/yhdPputative peptidase/DUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base T (90/86);  new base C (5/5);  total (96/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 189 total aligned reads displayed.

CGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCA  >  NC_000913/3392042‑3392339
                                                                                                                                                    |                                                                                                                                                      
cGTTAGTTACTGCCGTAGAGGATTTTTTACGGCTGACGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGCTAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTa                                                                                                                                                       >  1:742206/1‑150 (MQ=255)
  ttagttaCTGCAGTAGAGTATTTTTTCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                     <  2:1436568/150‑1 (MQ=255)
  ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                     >  1:1889176/1‑150 (MQ=255)
  ttagttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                     >  1:1217233/1‑150 (MQ=255)
     gttaCTGCAGTCGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGa                                                                                                                                                  <  2:1126984/150‑1 (MQ=255)
      ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGTTGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                                                 <  2:2125018/150‑1 (MQ=255)
      ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                                                                                                                                 <  2:277043/150‑1 (MQ=255)
      ttaCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATAAGGCGTTTACGCCGCATCCGAc                                                                                                                                                 <  2:1682484/150‑1 (MQ=18)
       taCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATAAGGCGTTTACGCCGCATCCGAca                                                                                                                                                <  2:449877/150‑2 (MQ=18)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAATACGAACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGAAAACGCCGTATCCGACCt                                                                                                                                               >  2:970399/1‑150 (MQ=18)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCt                                                                                                                                               >  1:1249478/1‑150 (MQ=255)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGGAAACGCGTTATCCGACCt                                                                                                                                               >  1:1288511/1‑150 (MQ=255)
        aCTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCATCTTAAGACGACCAGCGTCGAGTCAGCCACCGGCTGACGGATGCGCCGGAAACCCCTTCTCCGACCt                                                                                                                                               >  1:25316/1‑150 (MQ=18)
            cAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACgg                                                                                                                                           >  1:722005/1‑150 (MQ=255)
             cggagagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTGGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGt                                                                                                                                          <  2:1722797/147‑1 (MQ=255)
                agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                                                                       <  2:123811/150‑1 (MQ=255)
                agagGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                                                                                                                       <  2:134320/150‑1 (MQ=255)
                    gATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTAGCATCAGTCCACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAGGTTc                                                                                                                                   >  2:2302272/1‑150 (MQ=255)
                      ttttttCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCATACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCg                                                                                                                                 <  2:859034/150‑1 (MQ=255)
                       tttttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGt                                                                                                                               >  1:1278043/1‑150 (MQ=255)
                         tttCCGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                                             <  2:296810/150‑1 (MQ=255)
                         tttACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGttt                                                                                                                             <  2:427046/150‑1 (MQ=255)
                            ccggcggCCGGTGGCGGCGTGAACGCCCTATCCAGCCTACGGTTTTGTTCGGTTTTGTCGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTa                                                                                                                          <  1:2351204/149‑1 (MQ=14)
                                cTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                                                                                                                      >  2:349083/1‑150 (MQ=255)
                                 tGCCGGATGGGGCTTGACCGCCCTACCCACCATACGTTTTTGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                     <  1:1036752/150‑1 (MQ=14)
                                 tGCCGGATGCGGCGTTACCGCCCTACCCAGCCTACGTTTATGTTCCGGTTTGTTGGCCTTATAAGACGCACTGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                     <  1:2335898/150‑1 (MQ=14)
                                 tGCCGGATGCGGCGTTAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                     <  1:250280/150‑1 (MQ=255)
                                 tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCGGGTTTGTAGGCCGGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                     <  2:824562/150‑1 (MQ=255)
                                 tGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                     <  2:1279750/150‑1 (MQ=255)
                                 tGCCGGAGGCGGCGTGAACGCCCCACCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCCTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCt                                                                                                                     <  1:1150796/150‑1 (MQ=17)
                                          cggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACg                                                                                                            <  2:2169902/150‑1 (MQ=255)
                                           ggcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                                                                                                           >  1:1329102/1‑150 (MQ=255)
                                            gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                                                                          <  1:349083/150‑1 (MQ=255)
                                            gcgTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGca                                                                                                          <  1:349093/150‑1 (MQ=255)
                                                      ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATCAGACGCACAGCGTCGCa                                                                                                >  2:1610258/1‑150 (MQ=255)
                                                      ccTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCa                                                                                                >  2:1049628/1‑150 (MQ=255)
                                                       cTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt                                                                                               >  1:1764090/1‑150 (MQ=255)
                                                         acccACCCTAGGGTTATGGTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAACAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                             <  1:1397870/148‑1 (MQ=255)
                                                         aTCCAGCCTCCGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                             <  1:765925/150‑1 (MQ=255)
                                                         aTCCAGCCTACGGTTATGTTCCGTTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                             <  1:2177329/150‑1 (MQ=255)
                                                         aTCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCa                                                                                             <  1:1834319/150‑1 (MQ=255)
                                                                 caCGGTTAGGTTCCGGCTGGTAGGCCTGATAAGCCGCACCGCGTCGCATCTGGAAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTTATAAGACGCACAGCGTCGCATCAGGCAACgg                                                                                     <  2:220038/149‑1 (MQ=11)
                                                                    ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                  >  2:1618102/1‑150 (MQ=37)
                                                                    ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                  >  1:1495201/1‑150 (MQ=37)
                                                                    ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                  >  2:84203/1‑150 (MQ=37)
                                                                    ggTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTg                                                                                  >  1:917440/1‑150 (MQ=37)
                                                                    ggTTATGTTCCGGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGATCCGGGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCACCGGCTg                                                                                  >  2:2374669/1‑150 (MQ=11)
                                                                           ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                           >  1:1000698/1‑150 (MQ=35)
                                                                           ttCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATg                                                                           >  1:1000569/1‑150 (MQ=35)
                                                                              cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                                        <  2:1773126/150‑1 (MQ=33)
                                                                              cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcgg                                                                        >  1:1165594/1‑150 (MQ=18)
                                                                              cGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGATGTTGGCCTGTTAAGACGCACAGCGAAGCTTCAGGCCACGCCTGACGCAAgcgg                                                                        >  1:2168731/1‑150 (MQ=11)
                                                                               ggTTTGTGGGCCTGATACGACGCACAGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                       <  2:1156137/150‑1 (MQ=255)
                                                                               ggTTTGTAGGCCTGATAAGACGCACAGCGTCGCCTCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTCGGCCTGTTAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggc                                                                       <  2:1583552/150‑1 (MQ=255)
                                                                                gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                                                      >  1:1585725/1‑150 (MQ=32)
                                                                                gTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATgcggcg                                                                      >  1:1642347/1‑150 (MQ=17)
                                                                                       ggCCTGATACGCCGCACAGCGGCGCATCTGGCAACGGCGGGCGGAGGCGGCGTAAACGCCTTAGCCGACCTACGGTTATTTACCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                               <  2:1538305/150‑1 (MQ=11)
                                                                                       ggCCTGATAAGCCGCACCGGGGCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                               <  2:722005/150‑1 (MQ=11)
                                                                                       ggCCTGATAAGACGCACCGCGTCGCATACGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTACCCGACCTCCGTTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                               <  2:1848056/150‑1 (MQ=11)
                                                                                       ggCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGc                                                               <  2:2093083/150‑1 (MQ=34)
                                                                                        gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGcc                                                              >  2:2056040/1‑150 (MQ=34)
                                                                                        gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGcc                                                              >  2:2089913/1‑150 (MQ=34)
                                                                                        gCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTTAACGcc                                                              >  2:1396368/1‑150 (MQ=17)
                                                                                                ctccGCACTGCGGCGCAACGGGAAACGCCTGTCGTATGCGGCTTAAACGCCTTACCCGACATCCGTTTATGTTCCTTTTGTAGGCCTGATAAGACGCACCGCTTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                      <  2:617486/147‑1 (MQ=11)
                                                                                                acaCGCACCGCGTCGCATCTGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGTTTTTTTTCCTTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                      <  2:1050834/148‑1 (MQ=11)
                                                                                                aGACGCTCAGGTGCGCATCTGGCAACGGGTGTGGTATGCGGCGTAAACGCCTTATCCGACATAGGTTTATGTTCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                      <  2:93108/150‑1 (MQ=11)
                                                                                                aGACGCTCAGCTTCGCTTCTGTCAACGGATGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGAAACGTCTGCCGGATGCGGCGTAAACGCCTTTTCCGa                                                      <  2:2401204/150‑1 (MQ=11)
                                                                                                aGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGa                                                      >  1:2218557/1‑150 (MQ=37)
                                                                                                aGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCGGCCGGATGCGGCGTAAACGCCTTATCCGa                                                      >  1:324207/1‑150 (MQ=37)
                                                                                                 gACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                                                     <  2:1978801/150‑1 (MQ=37)
                                                                                                 gACGCACAGCGTCGCATCAGGCAACGGCGGTCGGATGCGGCGTAAACGCCTTATCCGACCTAGGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAc                                                     <  2:1978626/150‑1 (MQ=255)
                                                                                                  aCGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGAcc                                                    >  2:569688/1‑150 (MQ=37)
                                                                                                           gTCGCATCTGTCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATTAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                           <  2:1365802/150‑1 (MQ=255)
                                                                                                           gTCGCATCTGGCAAGGGGTGTGGGATGCGGCTTAAACGCATTACCCTCCATCGGGTTTTGTACCGTTTTTTGGCCTGATAAGCCGCACAGCGTCGAATATGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                           <  1:1417139/150‑1 (MQ=2)
                                                                                                           gTCGCATCGGGAACGGGCTGGCGGTGGCGGCTTAACCGCATTATCCGACCTCGGTTTTTGTTCGTTTTGTTGGCCTTATAAGACCCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTAt                                           <  1:544855/150‑1 (MQ=2)
                                                                                                            tCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATg                                          >  2:488330/1‑150 (MQ=37)
                                                                                                               cATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGGTATGTTc                                       >  1:584972/1‑150 (MQ=37)
                                                                                                                ttctgccAAGGGCTGTCGGTTGCGGCTTAAACGCCTTATCCTACCTAGGTTTTTGTCCCTTTTTTCGGCCTTATAAGACGCACCGCGTCGAATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                      <  1:463166/144‑1 (MQ=11)
                                                                                                                aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                      >  1:657465/1‑150 (MQ=38)
                                                                                                                aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTcc                                      >  1:953324/1‑150 (MQ=38)
                                                                                                                aTCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCAACGGGTATGTTcc                                      >  1:1925246/1‑150 (MQ=255)
                                                                                                                   aGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGGTCCGtg                                   >  1:1318988/1‑149 (MQ=255)
                                                                                                                          ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                 <  2:1584385/81‑1 (MQ=33)
                                                                                                                          ggCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc                                                                                                 >  1:1584385/1‑81 (MQ=33)
                                                                                                                           gCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGcc                           <  2:1733898/150‑1 (MQ=255)
                                                                                                                             tGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGGTTGTAGGCCTg                         >  1:1380817/1‑150 (MQ=255)
                                                                                                                              gccGGAGGCGGCGTAAACGCCTTACCCGACCTCCGGTTATGGACCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGa                        <  2:332182/148‑1 (MQ=14)
                                                                                                                               tCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCGTATCCGACCTACGGTTATGTTCCGTGTCTAGGCCTGAt                       >  2:1310399/1‑150 (MQ=255)
                                                                                                                               ggggttGCGGCGTAACCGCCTTATCCGCCGTTCGGTTATGTTGCTTTTTTCGGCCTGTTACGACGCCGAGCGGCGCATCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGAt                       <  2:411913/145‑1 (MQ=14)
                                                                                                                                cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTTGGCCTGATa                      >  1:1205927/1‑150 (MQ=255)
                                                                                                                                cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATa                      >  1:2195052/1‑150 (MQ=255)
                                                                                                                                cGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTGTGTAGGCCTGATa                      >  1:2057607/1‑150 (MQ=255)
                                                                                                                                  gATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTGATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAg                    >  1:2288848/1‑150 (MQ=255)
                                                                                                                                    taaggcgTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCGTTATCCGACCTCCGGTTAGGTTCCGTGTGTAGGCCTGATAAGAc                  >  2:1662842/4‑150 (MQ=14)
                                                                                                                                    taaggcgTTTACGCCGCATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGACGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGAc                  >  2:1683388/4‑150 (MQ=11)
                                                                                                                                      cggcgTAAACGCCTTATCCGTCCTCCGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGc                <  2:2380417/150‑1 (MQ=255)
                                                                                                                                           tAAACGCCTTATCCGACCTCGGTTTATGTTCGGTTTGTAGGCCTGATAAGACGCACCGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGc           <  1:874897/150‑1 (MQ=14)
                                                                                                                                                gCCTTATCCGACCTCGGTTTATTTCCGTTTTGTCGGCCTGATAAGACGCACAGCGTCGCTTCTGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc      <  1:2196376/150‑1 (MQ=11)
                                                                                                                                                cccTTATCCTACCTTCGTTTTTGTTCCTTTTGTTGGCCTGATAAGACGCCCCGCGTCGAATCAGCCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGc      <  1:416920/149‑1 (MQ=14)
                                                                                                                                                  cTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCAt    >  1:1472627/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CGTTAGTTACTGCAGTAGAGGATTTTTTACGGCTGCCGGATGCGGCGTGAACGCCCTATCCAGCCTACGGTTATGTTCCGGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCAGGCAACGGCTGCCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTATGTTCCGTTTGTAGGCCTGATAAGACGCACAGCGTCGCATCA  >  NC_000913/3392042‑3392339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: