Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 9.0% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 9.0% 269.4 / 4.8 117intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (28/76);  new base G (5/8);  total (33/84)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

ACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504707‑3505004
                                                                                                                                                    |                                                                                                                                                     
aCAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGtt                                                                                                                                                      <  2:930277/150‑1 (MQ=255)
aCAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGGTGACTTTTACATCCCGTACGtt                                                                                                                                                      >  2:2374156/1‑150 (MQ=255)
  aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                    >  1:867741/1‑150 (MQ=255)
     ccGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCt                                                                                                                                                 <  2:167072/150‑1 (MQ=255)
        gTGCTCGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCGCGTACGTTCCCCTCAc                                                                                                                                              <  2:1701042/150‑1 (MQ=255)
        gTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCAc                                                                                                                                              <  2:1701061/150‑1 (MQ=255)
               tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCGAAcc                                                                                                                                       >  1:1141995/1‑150 (MQ=255)
               tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACAACCaaaac                                                                                                                                       >  1:226373/1‑148 (MQ=255)
                 tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGGAGGCCGGAGAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAAcccg                                                                                                                                     >  1:1959368/1‑149 (MQ=255)
                     aaCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                 <  1:1191120/150‑1 (MQ=255)
                        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCGAAACctcaacccc                                                                                                                              >  1:430045/1‑144 (MQ=39)
                        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCGCCCGAACACACACCCCa                                                                                                                              >  2:2047394/1‑150 (MQ=39)
                        tcttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTGCCCCTCACCCTAACCCTCTccccc                                                                                                                              >  2:134153/1‑149 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCaaa                                                                                                                            >  1:2333096/1‑150 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaca                                                                                                                            >  1:613261/1‑148 (MQ=255)
                          ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCaaa                                                                                                                            >  1:613512/1‑150 (MQ=255)
                           tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTc                                                                                                                                                     >  2:1992556/1‑124 (MQ=255)
                           tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTc                                                                                                                                                     <  1:1992556/124‑1 (MQ=255)
                             tGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                         <  2:925081/150‑1 (MQ=255)
                             agCCGCGCCAATCGCATCAACTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAggg                                                                                                                         <  2:1131204/149‑1 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:320718/150‑1 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:758187/150‑1 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:759948/150‑1 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  1:760122/150‑1 (MQ=255)
                                 gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGa                                                                                                                     <  2:1643408/150‑1 (MQ=255)
                                   gcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  1:117538/150‑1 (MQ=39)
                                   gcCAATCGCATCACCTGAACTATTGATAATAAAAGACATTCACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  2:483392/150‑1 (MQ=38)
                                    ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAACGGGGCGAggg                                                                                                                  <  2:1412434/150‑1 (MQ=255)
                                    ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                  <  2:129724/150‑1 (MQ=255)
                                    ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                  <  1:2135747/150‑1 (MQ=255)
                                    ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCCCACCCCAACCCACTCCCCAAAGGGGCGAggg                                                                                                                  >  2:241985/1‑150 (MQ=255)
                                        tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAAACCACACCCCAAACGGGCGAGGGGAcc                                                                                                              >  1:1019178/1‑150 (MQ=38)
                                        tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAACCCACACCCCAAAGGGGCGAGGGGAcc                                                                                                              >  1:2207028/1‑150 (MQ=255)
                                        tCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACACCCAAACCCACAACCCCAAAGGGCGAGGGGAcc                                                                                                              >  1:1465844/1‑150 (MQ=38)
                                         cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCg                                                                                                             <  1:25012/150‑1 (MQ=255)
                                          gCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCTAACCCTCTCCCCCAAGGGGCGCGGGGACCGt                                                                                                            >  2:2215815/1‑150 (MQ=255)
                                          gCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCGCACCCGAACCCACTCCCCCAAGGGGCGAGGGGCCCgg                                                                                                            >  2:713118/1‑149 (MQ=38)
                                           cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                           <  1:694099/150‑1 (MQ=255)
                                           cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCGCCCCAAAGGGGCGAGGGGACCGtt                                                                                                           <  1:765197/150‑1 (MQ=255)
                                             tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  1:2167996/150‑1 (MQ=255)
                                               aCCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCCCACCCAAACCCACACCCCCAAAGGGCGCGGGGccccggccga                                                                                                       >  2:2049935/1‑143 (MQ=37)
                                               aCCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCGCCCCCAAACCCACAACCCCCACGGGCGCGGGGGcccgtccct                                                                                                       >  1:421874/1‑143 (MQ=25)
                                               aCCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATACGCCGATGATGGCGCGTTGAATTTTAAAAACCGTACGTTACCCACAAACTAAAACACAACCAAAACGGGGGTTGGGACCGTTAAGt                                                                                                       >  1:306646/1‑150 (MQ=255)
                                                ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTa                                                                                                      <  1:1377924/150‑1 (MQ=255)
                                                 cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                     <  1:1678252/150‑1 (MQ=255)
                                                 cTTAACGATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTAc                                                                                                     <  1:2369669/150‑1 (MQ=255)
                                                  ttAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCCAAGGGGCGGGGGGTCCCGTAAGTACg                                                                                                    >  1:1516640/1‑150 (MQ=38)
                                                     ccTATTGAGAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGaa                                                                                                 <  2:2415512/149‑1 (MQ=255)
                                                         ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTa                                                                                             <  1:235352/150‑1 (MQ=255)
                                                           gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAACCCACTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                           >  2:2299044/1‑150 (MQ=39)
                                                               ataaAAGACATTAACCGTAGGCCTGATAAGATGCGCCAGCTTCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:853100/150‑1 (MQ=255)
                                                               ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:853111/150‑1 (MQ=255)
                                                               ataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGa                                                                                       <  1:853113/150‑1 (MQ=255)
                                                                taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                      <  2:564865/150‑1 (MQ=255)
                                                                taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                      <  2:565151/150‑1 (MQ=255)
                                                                taaAAGACAGTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                      <  2:1750045/150‑1 (MQ=255)
                                                                    agtcATTAACCGTAGGCCGGATTTGATGCGCCAGCATAGAATCCGGCGATGCTGGCGCGTTTAATTTTTGATCCGGTACTTTCCACTCACCCTAACGCTCTCACCTAAGGGGCGAGGGGACCGTTCAGTACTGTATTTAGCTTGAGGGTg                                                                                  <  2:946240/147‑1 (MQ=255)
                                                                    aGACCTTACCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCCCCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  2:2284135/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  2:2392122/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:1256374/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:2187648/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:651781/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:1510943/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:775560/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:795110/150‑1 (MQ=255)
                                                                    aGACATTAACCGTAGGCCGGATAAGATGAGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:2187597/150‑1 (MQ=255)
                                                                    aGACATGAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:241908/150‑1 (MQ=255)
                                                                         ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                             <  2:221345/150‑1 (MQ=255)
                                                                         ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                             <  2:2360061/150‑1 (MQ=255)
                                                                         ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCACCGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTacc                                                                             <  1:782958/150‑3 (MQ=255)
                                                                              cGTCGGCCGGATAAGAGGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGCATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCCCCGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                        <  2:371678/150‑1 (MQ=255)
                                                                              cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCAGGCGCGTTGAATATTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                        <  2:1117739/150‑1 (MQ=255)
                                                                                tAGGCCGGATAAGATGCGCCAGCATCGCCTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTTTCCAGCAtt                                                                      <  2:2402677/150‑1 (MQ=255)
                                                                                tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  2:1051889/150‑1 (MQ=255)
                                                                                  ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaa                                                                    <  2:1369309/150‑1 (MQ=255)
                                                                                       gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                               <  2:2225634/150‑1 (MQ=255)
                                                                                       gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                               <  2:398613/150‑1 (MQ=255)
                                                                                                   cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCTAACCCAAACCCCAAAGGGGCGAGGGGACCGGTCAGTAAGGAAATTATCTTGAAGGTGGTATCCAGCAATAAATAATGGCCAAACtctc                                                   >  2:1653674/1‑150 (MQ=255)
                                                                                                   cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACTGGGAATACtctc                                                   >  2:1462912/1‑150 (MQ=255)
                                                                                                   cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAAACCACTACCCCAAGGGGCGCGGGGACCGTTCACTACGGGATTTAGCTTGTCGGTGCGATCCCGCACTAAATAAAGGCAATActcgc                                                   >  2:916103/1‑148 (MQ=255)
                                                                                                    aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  <  1:152066/150‑1 (MQ=255)
                                                                                                    aGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCa                                                  <  1:1219797/150‑1 (MQ=255)
                                                                                                           cttgcGGCGATGCTGGCGAGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGTGCGAGGGGACAGTTCAGTAAGGAATTTAGCTTGACGGTGCTATCCATCATTAAATACTGGCAATACTCTCACGCaaaa                                           <  2:1461932/146‑1 (MQ=39)
                                                                                                           catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaaa                                           <  2:1461960/150‑1 (MQ=255)
                                                                                                               cGGCGATGGTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGATTTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCTATACTCTCACGCAAAATCCg                                       <  2:446147/150‑1 (MQ=255)
                                                                                                               cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  2:1925731/150‑1 (MQ=255)
                                                                                                               cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  2:1082545/150‑1 (MQ=255)
                                                                                                               cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  2:476551/150‑1 (MQ=255)
                                                                                                                ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgc                                      <  2:2231929/150‑1 (MQ=255)
                                                                                                                  cGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                                    <  2:1608452/150‑1 (MQ=255)
                                                                                                                  cGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcag                                    <  2:1486245/150‑1 (MQ=255)
                                                                                                                         ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                             <  1:2332052/150‑1 (MQ=255)
                                                                                                                         ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                             <  2:2174745/150‑1 (MQ=255)
                                                                                                                         ggCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGtc                             <  1:1709185/150‑1 (MQ=255)
                                                                                                                               ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCa                       <  1:1351452/150‑1 (MQ=255)
                                                                                                                                            cccGTACGTTCCCCACACCCAAAACCACAAACCAAAGGGGCGAGGGGACCCTTAAGTAACGAATATAGCTTGACGGTGCTATCCAGCAATAAATAATTGCAAAAATATCACCCAAAATACGCACCAACGTCTCGTCACCCCTGCGCttgg          >  2:1133864/1‑148 (MQ=255)
                                                                                                                                               gTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGt       <  1:2071942/150‑1 (MQ=255)
                                                                                                                                                   gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTCACCGCTGCGATTCGGGTTAATg  >  2:1768641/1‑150 (MQ=255)
                                                                                                                                                    ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg  >  2:824758/1‑150 (MQ=255)
                                                                                                                                                    ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg  >  2:1651335/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504707‑3505004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: