Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,244,626 T→A 7.3% K150N (AAA→AAT malF ← maltose transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,244,6260TA 7.3% 428.1 / 2.2 134K150N (AAA→AATmalFmaltose transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (52/69);  new base A (11/2);  total (63/71)
Fisher's exact test for biased strand distribution p-value = 6.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGT  >  NC_000913/4244477‑4244775
                                                                                                                                                     |                                                                                                                                                     
gAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAAt                                                                                                                                                       <  1:634759/150‑1 (MQ=255)
gAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAAt                                                                                                                                                       <  2:2052600/150‑1 (MQ=255)
gAGCTCATCATCACTTTGGTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAAt                                                                                                                                                       <  2:978507/150‑1 (MQ=255)
    tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTGAGTTGCAGTTTTTGGTAGCCGCCAAATTTaa                                                                                                                                                   >  1:622572/1‑150 (MQ=255)
    tcatcatcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTGCAGTTTTTGCTCGCCGCCAAATTTaa                                                                                                                                                   >  1:1120835/1‑150 (MQ=255)
          tcaCTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTATTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGAGt                                                                                                                                             >  1:1351698/1‑150 (MQ=255)
             cTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCgg                                                                                                                                          >  2:30965/1‑150 (MQ=255)
              tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGGTTTTGCTCGCCGCCAAATTTAAAAGCGTCGga                                                                                                                                         >  2:406085/1‑150 (MQ=255)
              tttGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCGTTCAGTTGCAGTTTTTGCTCGCCGCCCAATTTAAAAGCGGCGga                                                                                                                                         >  2:1947589/1‑150 (MQ=255)
                tgttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGgaga                                                                                                                                       <  1:962301/150‑1 (MQ=255)
                 gttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTGAGTTGGAGTTTTTTCTGGGCGGCCAATTTAAAAGAGTGGGggag                                                                                                                                      >  1:1031496/1‑150 (MQ=255)
                  ttgCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAgg                                                                                                                                     <  1:1757675/150‑1 (MQ=255)
                    gCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTCAGTTGCAGTTTTTTGTCGCCGCCAAAATTAAAATCGTCGGTGAGGTa                                                                                                                                   >  1:1673162/1‑150 (MQ=255)
                     ccATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTCAGTTGCAGGTTTTTGTCGCCGCCCAAATTAAAATCGTCGGAGAGGTaa                                                                                                                                  >  1:2241178/1‑150 (MQ=255)
                        tCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAttt                                                                                                                               >  2:1114147/1‑150 (MQ=255)
                          cGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTCTTTAAGTTGCCGTTTTTGCTCGCCGCCCAATTTAAAAGCGTCGGAGAGGTAAttttt                                                                                                                             >  1:2278761/1‑150 (MQ=255)
                           ggCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTg                                                                                                                            <  1:461998/150‑1 (MQ=255)
                            gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                                                           <  1:1357705/150‑1 (MQ=255)
                            gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                                                           <  1:558411/150‑1 (MQ=255)
                            gCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCGTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGc                                                                                                                           >  1:1752291/1‑150 (MQ=255)
                             cAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGcc                                                                                                                          <  2:2146092/150‑1 (MQ=255)
                                   ggCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTc                                                                                                                    <  1:1620687/150‑1 (MQ=255)
                                    gCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGGGAGGTAATTTTTGCCGGTTTCg                                                                                                                   >  2:1693310/1‑150 (MQ=255)
                                    gCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCg                                                                                                                   >  2:571721/1‑150 (MQ=255)
                                            gTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTAAGTTTCAGTTTTTTGTAGCCGCCAAATTTAAAATAGTCGGAGAGGTAATTTTTTGAGGTTTGGCCGGCGCt                                                                                                           >  1:445811/1‑150 (MQ=255)
                                                  cAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAgcgc                                                                                                     <  1:345055/150‑1 (MQ=255)
                                                  cAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGATTTTGCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAgcgc                                                                                                     <  1:2224643/150‑1 (MQ=255)
                                                   aGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTGTTTGATTTGCGGTTTTTGGTCGCCGCCAAAATTAAAAACGTCGGAGAGGTAATTTTTTCCGGTTTAGCCCGCGCGGAGCGcc                                                                                                    >  1:2136508/1‑150 (MQ=255)
                                                        cTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGGTGGACTTTTTTCTAGCCGCCAAAAATAAAAAACAGGGAGTGGTAAATTTTTGCGGTTTATCCGGCCCGGAGCGccccgtg                                                                                               >  1:323640/1‑145 (MQ=255)
                                                        cTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTGTGTTTGAGTTTACGTTTTTTGTAGCCCCCCAAATTAAAAGAGGCGGAGAGGTAAATTTTTACGGTTTAGCCGGCGCGGAGCGCCacatg                                                                                               >  1:323504/1‑146 (MQ=255)
                                                               ttCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCa                                                                                        <  1:1332611/150‑1 (MQ=255)
                                                               gtCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCa                                                                                        <  2:1054261/149‑1 (MQ=255)
                                                               gtCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCa                                                                                        <  2:1054302/149‑1 (MQ=255)
                                                                     gTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgccc                                                                                  <  1:758397/150‑1 (MQ=255)
                                                                      tAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTAAGTTTGAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTAGCTGAGCGGCAGTTGCCACTCATagcccg                                                                                 >  2:1973493/1‑150 (MQ=255)
                                                                        aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgcc                                                                               <  1:84514/150‑1 (MQ=255)
                                                                        aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgcc                                                                               <  1:84479/150‑1 (MQ=255)
                                                                        aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgcc                                                                               <  2:1311560/150‑1 (MQ=255)
                                                                        aTCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATcgcccgcc                                                                               <  2:1561793/150‑1 (MQ=255)
                                                                         tCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCccgccg                                                                              <  2:555040/150‑1 (MQ=255)
                                                                           aCGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAAATTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCggg                                                                            >  2:2119654/1‑150 (MQ=255)
                                                                           aCGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTGCTTTGCGGTGCAGTTTTTTCGCCCCGCCAAAATTAAAAGCGTCGGAGCTGTAAGTTTTGCCGGTTTCGCCGGCGCTGAGCGCCAGTTTGCACTCAACGCCCGCCggg                                                                            >  2:1722825/1‑150 (MQ=255)
                                                                            cgcgCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGt                                                                           <  2:307108/150‑1 (MQ=255)
                                                                                 aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTTGAGTTTTTTCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTTATTTTTTCCGGTTTAGCCGTCGCTGAGCGCCCGTTGCCACTAATCGCCCCCCGGGTAAAGa                                                                      >  2:648512/1‑150 (MQ=255)
                                                                                 aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGa                                                                      <  2:2340504/150‑1 (MQ=255)
                                                                                 aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGa                                                                      >  2:1983582/1‑150 (MQ=255)
                                                                                 aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGa                                                                      <  2:439608/150‑1 (MQ=255)
                                                                                 aGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGa                                                                      <  2:1149121/150‑1 (MQ=255)
                                                                                   aTTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGAcc                                                                    <  2:1572683/150‑1 (MQ=255)
                                                                                          cgTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTta                                                             <  2:323640/150‑1 (MQ=255)
                                                                                            ttCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTtata                                                           <  1:1747861/150‑1 (MQ=255)
                                                                                               gCCTTCGGGCTGGGCGGTCGTTTCGTTCAGTTGCCGTTTTTGCTAGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTTCCGGTTTCGCCCGCGCTGAGCGCCCGTTGCCCCGCATCGCCCCCCCGGTAAAGACCAAAGTTATAGGt                                                        >  2:614420/1‑150 (MQ=255)
                                                                                                  ttCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTTCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACACATCGCCCGCCGGGTAAAGACCAAAGTTATAGGtttt                                                     >  2:2340532/1‑150 (MQ=255)
                                                                                                    cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGc                                                   <  2:1670505/150‑1 (MQ=255)
                                                                                                    cGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGc                                                   <  1:248886/150‑1 (MQ=255)
                                                                                                    cGGGCTGGGCGGTCGTTTCTTTCAGTTGCACTTTTTTCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAAATTTTGCCGGTTTAGCCGACGCTGAGCGCCAGTTTCCACACAACGCCCGCCGGGTAAATAACAAAGTTATAGGtttttc                                                   >  2:1198815/1‑148 (MQ=255)
                                                                                                     gggCTGGGCGGTCGTTTGTTTGAGTTGGAGTTTTTTCTCGCCGCCAAATTTAAAATGGTGGGAGAGGTAATTTTTTGCGGTTTAGCCGTGGCTTTGCGCCATTTGGCACTCATAGCCCGCCGGGTAAATAGCAAAGTTATTGGtttttga                                                  >  2:985049/1‑147 (MQ=255)
                                                                                                                 cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  2:2363470/150‑1 (MQ=255)
                                                                                                                 cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  1:652955/150‑1 (MQ=255)
                                                                                                                 cGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGa                                      <  1:2088095/150‑1 (MQ=255)
                                                                                                                     tctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGa                                  >  1:1056955/1‑150 (MQ=255)
                                                                                                                     tctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGa                                  >  1:1848971/1‑150 (MQ=255)
                                                                                                                      ctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGAt                                 <  1:556895/150‑1 (MQ=255)
                                                                                                                      ctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGAt                                 <  2:2241178/150‑1 (MQ=255)
                                                                                                                      ctttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGAt                                 <  2:1923909/150‑1 (MQ=255)
                                                                                                                      ctttcAGTTGCAGTTTGTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGAt                                 <  1:1278412/150‑1 (MQ=255)
                                                                                                                       tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  1:832540/1‑150 (MQ=255)
                                                                                                                       tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  2:72555/1‑150 (MQ=255)
                                                                                                                       tttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATc                                >  2:1140990/1‑150 (MQ=255)
                                                                                                                        ttcAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCt                               <  1:1973493/150‑1 (MQ=255)
                                                                                                                          cAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaa                             <  1:1755480/150‑1 (MQ=255)
                                                                                                                           aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaac                            <  1:2353662/150‑1 (MQ=255)
                                                                                                                           aGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaac                            <  1:2078463/150‑1 (MQ=255)
                                                                                                                            gTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaaca                           >  2:748686/1‑150 (MQ=255)
                                                                                                                            gTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaaca                           <  2:498275/150‑1 (MQ=255)
                                                                                                                            gTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaaca                           <  2:1673162/150‑1 (MQ=255)
                                                                                                                            gTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaaca                           >  1:1517329/1‑150 (MQ=255)
                                                                                                                            gTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaaca                           >  1:179240/1‑150 (MQ=255)
                                                                                                                            gTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaaca                           >  2:1615212/1‑150 (MQ=255)
                                                                                                                             ttGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa                          <  2:847011/150‑1 (MQ=255)
                                                                                                                             ttGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaa                          <  2:1713354/150‑1 (MQ=255)
                                                                                                                              tGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTaacaac                         <  1:2185570/150‑1 (MQ=255)
                                                                                                                               gCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAacaaca                        >  1:1868872/1‑150 (MQ=255)
                                                                                                                               gCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAacaaca                        >  1:1066178/1‑150 (MQ=255)
                                                                                                                                   tttttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGATAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTc                    >  1:1677956/1‑150 (MQ=255)
                                                                                                                                     tttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGATCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCt                  <  2:2017589/150‑1 (MQ=255)
                                                                                                                                      ttGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTg                 <  2:2036747/150‑1 (MQ=255)
                                                                                                                                      tagCTCGCCGCCAAAATTAAAAGCGTCGGAGAGGTAATTATTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACACATCGCCCGCCGGGAAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTg                 <  2:1474612/148‑1 (MQ=255)
                                                                                                                                        gCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgcg               <  2:1530746/150‑1 (MQ=255)
                                                                                                                                        gCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTgcg               <  2:34315/150‑1 (MQ=255)
                                                                                                                                          tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCa             >  1:1562131/1‑150 (MQ=255)
                                                                                                                                          tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCa             >  2:1395392/1‑150 (MQ=255)
                                                                                                                                          tCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCa             >  1:2256472/1‑150 (MQ=255)
                                                                                                                                            gccgccAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACg           >  1:2385289/1‑150 (MQ=255)
                                                                                                                                                 cAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaa      <  2:747893/150‑1 (MQ=255)
                                                                                                                                                  aaaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaa     >  1:1489413/1‑150 (MQ=255)
                                                                                                                                                  aaaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaa     >  1:1096935/1‑150 (MQ=255)
                                                                                                                                                  aaaTTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCaaa     >  1:1096907/1‑150 (MQ=255)
                                                                                                                                                     tttAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGt  <  1:2159282/150‑1 (MQ=255)
                                                                                                                                                     tttAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGt  <  1:481936/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAGCTCATCATCACTTTGTTGCCATCCGGCAGAATGGCGGTAATGTCACTCAGCGCCTGACGATTCTGGGTAATCACGCGCAGATTCGCGCGTTCGCCTTCGGGCTGGGCGGTCGTTTCTTTCAGTTGCAGTTTTTGCTCGCCGCCAAATTTAAAAGCGTCGGAGAGGTAATTTTTGCCGGTTTCGCCGTCGCTGAGCGCCAGTTGCCACTCATCGCCCGCCGGGTAAAGACCAAAGTTATAGGTTTTGCCTGCTTGCCAGGAGCGATCTAACAACACTTCCTGCGCACGTTCAAAAGT  >  NC_000913/4244477‑4244775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: