Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,370,148 C→T 5.1% G89S (GGC→AGC)  yjeH ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,370,1480CT 5.1% 549.5 / 11.7 156G89S (GGC→AGC) yjeHputative transporter
Reads supporting (aligned to +/- strand):  ref base C (74/74);  new base T (4/4);  total (78/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 163 total aligned reads displayed.

TACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTA  >  NC_000913/4370001‑4370296
                                                                                                                                                   |                                                                                                                                                    
tACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCgg                                                                                                                                                    >  2:1650318/1‑150 (MQ=255)
 aCCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGt                                                                                                                                                   <  1:1268835/150‑1 (MQ=255)
   cGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGACTGACCGATAAAAACAGCCAGCCGGTGa                                                                                                                                                 >  2:417186/1‑150 (MQ=255)
        taCCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGc                                                                                                                                            >  2:2063812/1‑150 (MQ=255)
          ccACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGTCCGATAAAATCAGCCAGCCGGTGACTCGCTc                                                                                                                                          >  1:466548/1‑150 (MQ=255)
          ccACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTc                                                                                                                                          >  1:259744/1‑150 (MQ=255)
              acCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGc                                                                                                                                      <  2:1536741/150‑1 (MQ=255)
              acCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGc                                                                                                                                      <  2:1845930/150‑1 (MQ=255)
               ccagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGcc                                                                                                                                     >  1:1817897/1‑150 (MQ=255)
                cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGACTGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCcg                                                                                                                                    >  2:1317793/1‑150 (MQ=255)
                cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCcg                                                                                                                                    >  1:710738/1‑150 (MQ=255)
                cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCcg                                                                                                                                    >  2:1317968/1‑150 (MQ=255)
                cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCcg                                                                                                                                    >  1:1708626/1‑150 (MQ=255)
                cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCcg                                                                                                                                    >  1:713248/1‑150 (MQ=255)
                cagcgccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAAGTTGTAGTGCGGCAGGCACACCCACGGGAATGACCGATAAACACAGCCAGCCGGTGACTCGCTCAAGCcg                                                                                                                                    >  2:1462132/1‑150 (MQ=255)
                 agcgccagcgTACCGAGTTCTGCCAACAACAGGTGCCAGCTATGCCAGCCAACCATCGCCGGGCCGAACCCGGCGGCAATTTGGAGTGCGGCAGGCAAACCCACGGGAATGACCGAGAAACACAGCCAGCCGGTGACTCGCTCAAGCcgc                                                                                                                                   <  2:475791/150‑1 (MQ=255)
                    gccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGaa                                                                                                                                <  1:1960005/150‑1 (MQ=255)
                    gccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGaa                                                                                                                                <  1:1603645/150‑1 (MQ=255)
                    gccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGaa                                                                                                                                <  1:648833/150‑1 (MQ=255)
                    gccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGaa                                                                                                                                <  2:1852801/150‑1 (MQ=255)
                     ccagcgTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAAc                                                                                                                               <  1:542726/150‑1 (MQ=255)
                             ccGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGcc                                                                                                                       >  1:943947/1‑150 (MQ=255)
                              cGATTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCAGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAATCAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCa                                                                                                                      >  2:1711919/1‑150 (MQ=255)
                                  ttCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATAcc                                                                                                                  >  2:1569184/1‑150 (MQ=255)
                                  ttCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATAcc                                                                                                                  >  2:804485/1‑150 (MQ=255)
                                    cTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCTGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcga                                                                                                                >  1:1733022/1‑150 (MQ=255)
                                    cTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCTGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcga                                                                                                                >  1:1439199/1‑150 (MQ=255)
                                    cTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCTGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcga                                                                                                                >  1:2214940/1‑150 (MQ=255)
                                    cTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCTGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcga                                                                                                                >  1:2216713/1‑150 (MQ=255)
                                     tGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcgac                                                                                                               >  1:1416876/1‑150 (MQ=255)
                                       ccAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACcgacga                                                                                                             >  1:1503403/1‑150 (MQ=255)
                                            aacaGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgc                                                                                                        <  1:901844/150‑1 (MQ=255)
                                            aacaGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgc                                                                                                        <  2:1818541/150‑1 (MQ=255)
                                             acaGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgcg                                                                                                       <  2:921382/150‑1 (MQ=255)
                                             acaGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgcg                                                                                                       <  2:1915725/150‑1 (MQ=255)
                                              caGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgcgc                                                                                                      <  2:662640/150‑1 (MQ=255)
                                              caGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTgcgc                                                                                                      >  1:413751/1‑150 (MQ=255)
                                                 ttGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTGGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGGCGAAGTGCGCGAc                                                                                                   >  2:685576/1‑150 (MQ=255)
                                                 ttGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAc                                                                                                   >  2:1510676/1‑150 (MQ=255)
                                                 ttGCCAGCTATGCCAGCCAAACAACGCCTGGCCGAACCCGGAGGCAATTTGTTGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCAATCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGcgcgtc                                                                                                   >  2:1998562/1‑148 (MQ=255)
                                                    ccAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCACTTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGACCCAAACGCCATACCGACGAAGTGCGCGAcgcc                                                                                                >  1:269144/1‑150 (MQ=255)
                                                    ccAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgcc                                                                                                <  1:2014254/150‑1 (MQ=255)
                                                    ccAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgcc                                                                                                <  1:2276559/150‑1 (MQ=255)
                                                    ccAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgcc                                                                                                >  1:451211/1‑150 (MQ=255)
                                                    ccAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgcc                                                                                                <  1:1168525/150‑1 (MQ=255)
                                                    ccAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgcc                                                                                                >  1:829045/1‑150 (MQ=255)
                                                      aGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgccgc                                                                                              >  2:2370717/1‑150 (MQ=255)
                                                      aGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgccgc                                                                                              <  1:1185457/150‑1 (MQ=255)
                                                      aGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgccgc                                                                                              >  1:142254/1‑150 (MQ=255)
                                                      aGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGAcgccgc                                                                                              >  1:1560405/1‑150 (MQ=255)
                                                        cTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCt                                                                                            <  1:1757896/150‑1 (MQ=255)
                                                             ccagccaAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCt                                                                                       >  1:757337/1‑150 (MQ=255)
                                                             ccagccaAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCt                                                                                       >  1:2308750/1‑150 (MQ=255)
                                                              cagccaAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTg                                                                                      >  2:1224249/1‑150 (MQ=255)
                                                                  caAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGAt                                                                                  >  1:1007000/1‑150 (MQ=255)
                                                                  caAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGAt                                                                                  >  1:1007008/1‑150 (MQ=255)
                                                                  caAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGAt                                                                                  >  1:952906/1‑150 (MQ=255)
                                                                     aCATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATa                                                                                                                    >  1:925218/1‑113 (MQ=255)
                                                                     aCATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGt                                                                               <  2:1302861/150‑1 (MQ=255)
                                                                      cATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTg                                                                              <  1:1656937/150‑1 (MQ=255)
                                                                      cATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTg                                                                              <  1:1656929/150‑1 (MQ=255)
                                                                       aTCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTgg                                                                             <  1:50269/150‑1 (MQ=255)
                                                                         cGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTggg                                                                                    >  1:902705/1‑141 (MQ=255)
                                                                         cGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTggg                                                                                    <  2:902624/141‑1 (MQ=255)
                                                                           ccTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGAc                                                                         <  1:2029673/150‑1 (MQ=255)
                                                                           ccTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGAc                                                                         <  2:1356867/150‑1 (MQ=255)
                                                                            cTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGCCTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGAcc                                                                        >  1:1464309/1‑150 (MQ=255)
                                                                            cTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGAcc                                                                        >  1:1331022/1‑150 (MQ=255)
                                                                            cTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGAcc                                                                        >  1:1464308/1‑150 (MQ=255)
                                                                              ggCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCa                                                                      <  1:464667/150‑1 (MQ=255)
                                                                                 cGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGaa                                                                   <  1:123332/150‑1 (MQ=255)
                                                                                   aaCCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATc                                                                 <  1:2303584/150‑1 (MQ=255)
                                                                                     cccGGCGGCAATTTGTAGTGCAGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGc                                                               <  1:344567/150‑1 (MQ=255)
                                                                                      ccGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCa                                                              >  2:222721/1‑150 (MQ=255)
                                                                                       cggcggCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCaa                                                             <  2:515132/150‑1 (MQ=255)
                                                                                       cggcggCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCaa                                                             <  2:8622/150‑1 (MQ=255)
                                                                                       cggcggCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCaa                                                             <  2:197097/150‑1 (MQ=255)
                                                                                       cggcggCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCaa                                                             <  1:1935612/150‑1 (MQ=255)
                                                                                       cggcggCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCaa                                                             <  1:1293349/150‑1 (MQ=255)
                                                                                                  tGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACAcaatcgca                                                  <  2:1117494/150‑1 (MQ=255)
                                                                                                  tGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACAcaatcgca                                                  <  2:63056/150‑1 (MQ=255)
                                                                                                      gTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACAcaatcgcaatcg                                              >  2:1428340/1‑150 (MQ=255)
                                                                                                        gCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg                                            <  1:1243524/150‑1 (MQ=255)
                                                                                                        gCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg                                            <  1:1240614/150‑1 (MQ=255)
                                                                                                        gCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCggg                                            <  2:574853/150‑1 (MQ=255)
                                                                                                                 aaaCCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAAGCGGGAACACTAAg                                   >  2:1510431/1‑150 (MQ=255)
                                                                                                                   aCCCACGGGAATGACCGATAAAAACAGCCAGCTGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGAt                                 <  2:1733022/150‑1 (MQ=255)
                                                                                                                   aCCCACGGGAATGACCGATAAAAACAGCCAGCTGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGAt                                 <  2:2216713/150‑1 (MQ=255)
                                                                                                                     ccACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATaa                               <  1:194002/150‑1 (MQ=255)
                                                                                                                       aCGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATc                             <  1:377222/150‑1 (MQ=255)
                                                                                                                          ggAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCaaa                          <  1:411708/150‑1 (MQ=255)
                                                                                                                          ggAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCaaa                          >  1:348522/1‑150 (MQ=255)
                                                                                                                               gACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACggg                     <  1:2370717/150‑1 (MQ=255)
                                                                                                                                   gATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAAt                              <  2:142429/137‑1 (MQ=255)
                                                                                                                                     tAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGc               >  1:574669/1‑150 (MQ=255)
                                                                                                                                     tAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGc               >  1:981124/1‑150 (MQ=255)
                                                                                                                                              ccagccGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCt      <  1:374605/150‑1 (MQ=255)
                                                                                                                                              ccagccGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCt      >  1:344895/1‑150 (MQ=255)
                                                                                                                                              ccagccGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCt      >  2:2137714/1‑150 (MQ=255)
                                                                                                                                                 gccGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGtt   <  2:2280453/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TACCGATATACCACACCAGCGCCAGCGTACCGAGTTCTGCCAACAACAGTTGCCAGCTATGCCAGCCAAACATCGCCTGGCCGAACCCGGCGGCAATTTGTAGTGCGGCAGGCAAACCCACGGGAATGACCGATAAAAACAGCCAGCCGGTGACTCGCTCAAGCCGCGAACCAAACGCCATACCGACGAAGTGCGCGACGCCGCCTGCGCTGGGATAGTGGCGACCCAGAATCGCAAACACAATCGCAATCGGGAACACTAAGATAATCAAAACGGGCCACGCCCACAGGCTGTTA  >  NC_000913/4370001‑4370296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑