Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,517,430 C→G 5.0% W96C (TGG→TGC fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,517,4300CG 5.0% 737.0 / 24.4 199W96C (TGG→TGCfecRanti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
Reads supporting (aligned to +/- strand):  ref base C (84/105);  new base G (5/5);  total (89/110)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 204 total aligned reads displayed.

CGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTG  >  NC_000913/4517281‑4517579
                                                                                                                                                     |                                                                                                                                                     
cgcTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGc                                                                                                                                                       <  1:1114519/150‑1 (MQ=255)
cgcTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGc                                                                                                                                                       <  1:1230902/150‑1 (MQ=255)
    gATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGc                                                                                                                                                   <  2:2031811/150‑1 (MQ=255)
    gATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGc                                                                                                                                                   <  1:517538/150‑1 (MQ=255)
      tGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCt                                                                                                                                                 <  2:426641/150‑1 (MQ=255)
       gcgcATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTc                                                                                                                                                <  1:1671093/150‑1 (MQ=255)
        cgcATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTcc                                                                                                                                               <  1:2074585/150‑1 (MQ=255)
        cgcATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTcc                                                                                                                                               <  1:1910783/150‑1 (MQ=255)
        cgcATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTcc                                                                                                                                               <  1:9716/150‑1 (MQ=255)
              aaaaCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGGCAGCCTCCGCCAgc                                                                                                                                         >  1:856290/1‑150 (MQ=255)
              aaaaCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGGCAGCCTCCGCCAgc                                                                                                                                         >  1:1829050/1‑150 (MQ=255)
              aaaaCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGGCAGCCTCCGCCAgc                                                                                                                                         >  2:232270/1‑150 (MQ=255)
              aaaaCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAgc                                                                                                                                         >  2:1120191/1‑150 (MQ=255)
              aaaaCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAgc                                                                                                                                         >  2:2076420/1‑150 (MQ=255)
               aaaCGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAgcg                                                                                                                                        >  2:1301172/1‑150 (MQ=255)
               aaaCGCACATCCGCCGCGCTGTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTGTCCGACTGCCAGAGCTGCCAGCCTCCGCCAgcg                                                                                                                                        >  2:1147440/1‑150 (MQ=255)
                   gCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGa                                                                                                                                    <  1:686227/150‑1 (MQ=255)
                   gCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGa                                                                                                                                    >  2:1639093/1‑150 (MQ=255)
                     acaTCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTACCAGCCTCCGCCAGCGCCGAGc                                                                                                                                  >  1:612616/1‑150 (MQ=255)
                      caTCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCa                                                                                                                                 <  1:1733120/150‑1 (MQ=255)
                      caTCCGCCGCGCTTTGGGTAATCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCa                                                                                                                                 <  1:658184/150‑1 (MQ=255)
                       aTCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCaa                                                                                                                                <  1:1006403/150‑1 (MQ=255)
                           gccgcGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAAcagc                                                                                                                            >  1:919640/1‑150 (MQ=255)
                           gccgcGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAAcagc                                                                                                                            >  1:2245768/1‑150 (MQ=255)
                              gcgcTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGt                                                                                                                         <  1:339954/150‑1 (MQ=255)
                              gcgcTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGt                                                                                                                         <  1:1259276/150‑1 (MQ=255)
                              gcgcTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTACCAGCCTCCGCCAGCGCCGAGCAACAGCAGt                                                                                                                         <  2:2206337/150‑1 (MQ=255)
                              gcgcTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTACCAGCCTCCGCCAGCGCCGAGCAACAGCAGt                                                                                                                         <  2:1379585/150‑1 (MQ=255)
                                  tttGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATc                                                                                                                     >  2:2172814/1‑150 (MQ=255)
                                    tGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCt                                                                                                                   <  1:1635443/150‑1 (MQ=255)
                                     gggTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCtt                                                                                                                  <  1:1544068/150‑1 (MQ=255)
                                      ggTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCttt                                                                                                                 >  2:702934/1‑150 (MQ=255)
                                      ggTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCttt                                                                                                                 <  2:2380179/150‑1 (MQ=255)
                                      ggTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCttt                                                                                                                 <  2:1836166/150‑1 (MQ=255)
                                       gTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTtc                                                                                                                <  2:237074/150‑1 (MQ=255)
                                       gTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTtc                                                                                                                <  2:623414/150‑1 (MQ=255)
                                       gTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTtc                                                                                                                <  1:418242/150‑1 (MQ=255)
                                       gTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTtc                                                                                                                <  1:475339/150‑1 (MQ=255)
                                         aTTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTtcat                                                                                                              >  1:2183009/1‑150 (MQ=255)
                                              gCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTGTCATCACGt                                                                                                         >  2:1508095/1‑150 (MQ=255)
                                               cGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTg                                                                                                        >  2:1108204/1‑150 (MQ=255)
                                                 tGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGCCTGCCAGAGCTGCCAGCCTCCGCCGGCGCCGCGCGACAGCGGGACTGCGTTCATCTCGTgtg                                                                                                      >  1:1356503/1‑148 (MQ=255)
                                                 tGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGAc                                                                                                      >  1:890976/1‑150 (MQ=255)
                                                    gCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGAcggc                                                                                                   <  2:2387051/150‑1 (MQ=255)
                                                     cAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGAcggcg                                                                                                  >  2:1760028/1‑150 (MQ=255)
                                                       gggAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCggg                                                                                                <  2:2310087/150‑1 (MQ=255)
                                                              gataaccAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCg                                                                                         >  2:1681326/6‑150 (MQ=255)
                                                              gataaccAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCg                                                                                         >  2:592598/6‑150 (MQ=255)
                                                              aTCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCg                                                                                         >  1:365614/1‑150 (MQ=255)
                                                               tCTTCCAGGTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCgg                                                                                        <  1:2300204/150‑1 (MQ=255)
                                                                cTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCggg                                                                                       <  1:1030911/150‑1 (MQ=255)
                                                                      gTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGt                                                                                 <  2:815511/150‑1 (MQ=255)
                                                                           tGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAAc                                                                            >  1:1476380/1‑150 (MQ=255)
                                                                           tGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAAc                                                                            >  1:1985620/1‑150 (MQ=255)
                                                                               ggCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGccc                                                                        >  1:776753/1‑150 (MQ=255)
                                                                               ggCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGccc                                                                        >  2:1256155/1‑150 (MQ=255)
                                                                               ggCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGccc                                                                        >  2:773507/1‑150 (MQ=255)
                                                                                 cTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCgg                                                                      >  2:1206050/1‑150 (MQ=255)
                                                                                    aCTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTACCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTg                                                                   <  2:612616/150‑1 (MQ=255)
                                                                                      tgtgCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGc                                                                 >  2:395653/1‑150 (MQ=255)
                                                                                       gtgCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCa                                                                >  2:478680/1‑150 (MQ=255)
                                                                                          ccTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACg                                                             >  1:897950/1‑150 (MQ=255)
                                                                                             ttGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCg                                                          >  2:1928977/1‑150 (MQ=255)
                                                                                               ggCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGcc                                                        <  1:1224177/150‑1 (MQ=255)
                                                                                                   gtgcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGc                                                    <  1:94570/150‑1 (MQ=255)
                                                                                                   gtgcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGc                                                    <  1:716891/150‑1 (MQ=255)
                                                                                                   gtgcggtAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGc                                                    <  1:2340071/150‑1 (MQ=255)
                                                                                                             cTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCaa                                          >  2:327440/1‑150 (MQ=255)
                                                                                                               gCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGc                                        <  1:18414/150‑1 (MQ=255)
                                                                                                                 ccGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTg                                      <  1:1206050/150‑1 (MQ=255)
                                                                                                                 ccGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTg                                      >  1:2102990/1‑150 (MQ=255)
                                                                                                                   gCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGt                                    >  1:45894/1‑150 (MQ=255)
                                                                                                                    cAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGtt                                   <  2:700695/150‑1 (MQ=255)
                                                                                                                    cAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGtt                                   <  2:2316303/150‑1 (MQ=255)
                                                                                                                     aGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTg                                  >  2:231894/1‑150 (MQ=255)
                                                                                                                         cTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCa                              <  2:1863068/150‑1 (MQ=255)
                                                                                                                         cTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCa                              <  2:550113/150‑1 (MQ=255)
                                                                                                                            cGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGt                           <  2:6483/150‑1 (MQ=255)
                                                                                                                              ccGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGTTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGttt                         >  2:1586836/1‑150 (MQ=255)
                                                                                                                               cGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGtttt                        >  1:1020554/1‑150 (MQ=255)
                                                                                                                               cGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGtttt                        <  1:1301172/150‑1 (MQ=255)
                                                                                                                               cGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGtttt                        <  1:1731374/150‑1 (MQ=255)
                                                                                                                               cGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGtttt                        >  1:1843375/1‑150 (MQ=255)
                                                                                                                               cGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGtttt                        >  1:2239504/1‑150 (MQ=255)
                                                                                                                                  tttCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCaa                     >  1:801223/1‑150 (MQ=255)
                                                                                                                                   ttCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAAc                    <  1:1098734/150‑1 (MQ=255)
                                                                                                                                      cGACTGCCAGAGCTGGCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctg                 <  2:1829050/150‑1 (MQ=255)
                                                                                                                                      cGACTGCCAGAGCTGGCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTGAAAACctg                 <  1:232073/150‑1 (MQ=255)
                                                                                                                                            ccAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGTTGCCa           >  1:1863779/1‑150 (MQ=255)
                                                                                                                                            ccAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCa           <  1:216906/150‑1 (MQ=255)
                                                                                                                                            ccAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCa           >  1:2248618/1‑150 (MQ=255)
                                                                                                                                              agagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAgg         >  2:521794/1‑150 (MQ=255)
                                                                                                                                              agagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAgg         <  2:846033/150‑1 (MQ=255)
                                                                                                                                               gagCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGc        >  2:1806607/1‑150 (MQ=255)
                                                                                                                                                  cTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCa     <  1:587007/150‑1 (MQ=255)
                                                                                                                                                    gCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACt   <  1:2059632/150‑1 (MQ=255)
                                                                                                                                                    gCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACt   <  1:2022322/150‑1 (MQ=255)
                                                                                                                                                    gCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACt   <  1:1984361/150‑1 (MQ=255)
                                                                                                                                                    gCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACt   <  1:393741/150‑1 (MQ=255)
                                                                                                                                                     ccAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTg  >  2:2221578/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCTGATGCGCATCAAAACGCACATCCGCCGCGCTTTGGGTATTCAGCGTGAGCAGGGAGCCATCTTCCAGTTGCTGACGGCTGACTGTGCCTTTGGCGGTGCGGTAATCTGCCCGCAGACCTTCGCCGGTTTCCGACTGCCAGAGCTGCCAGCCTCCGCCAGCGCCGAGCAACAGCAGTAATCCTTTCATCACGTGACGGCGGGTGAGGCGGGTATCGTGCAACGCCCGGCTGGCAACGTCGCCAGGCACACCGCCAAGCTGGTTGCGCAGGTTTTCAACCTGCTGCCAGGCCCACTG  >  NC_000913/4517281‑4517579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: