Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 645,069 G→A 5.3% intergenic (‑66/+48) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913645,0690GA 5.3% 532.6 / 11.4 154intergenic (‑66/+48)rna/citTribonuclease I/citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base G (72/72);  new base A (6/2);  total (78/76)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 156 total aligned reads displayed.

TGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCA  >  NC_000913/644920‑645217
                                                                                                                                                     |                                                                                                                                                       
tGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTg                                                                                                                                                         <  1:2345366/150‑1 (MQ=255)
 gCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGACGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTgg                                                                                                                                                        <  1:2000127/150‑1 (MQ=255)
     gCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCtt                                                                                                                                                    >  2:747820/1‑150 (MQ=255)
       aaCGCTAAGGCGTTGGCAGAATAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTAc                                                                                                                                                  >  1:1827133/1‑150 (MQ=255)
         cGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCt                                                                                                                                                <  1:303780/150‑1 (MQ=255)
             aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                            >  2:2275173/1‑150 (MQ=255)
             aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                            >  2:1558988/1‑150 (MQ=255)
             aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                            <  2:6273/150‑1 (MQ=255)
             aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                            <  2:929871/150‑1 (MQ=255)
                  gTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCATCGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACTCTGAATACTATCAATTAAATCATAAAATAGCCCGCCAGGTGGGCTTACGTGGGGGGC‑‑‑Gt                                                                                                                                    <  1:1444714/150‑1 (MQ=255)
                     ggCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtgat                                                                                                                                 >  2:1661994/1‑150 (MQ=255)
                       cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtgatga                                                                                                                               >  1:1091289/1‑150 (MQ=255)
                        agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTgatgat                                                                                                                              >  1:2100137/1‑150 (MQ=255)
                         gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                             >  2:866295/1‑150 (MQ=255)
                         gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                             >  2:840342/1‑150 (MQ=255)
                          aagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAttt                                                                                                                            >  1:266125/1‑150 (MQ=255)
                            gagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTAt                                                                                                                          <  1:148844/150‑1 (MQ=255)
                                       cAGAGAAACCGCGAGCTACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTgg                                                                                                               <  1:203706/150‑1 (MQ=255)
                                       cAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTgg                                                                                                               >  1:133037/1‑150 (MQ=255)
                                        agagAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGc                                                                                                              >  1:1297185/1‑150 (MQ=255)
                                          agaAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGa                                                                                                            >  1:2395543/1‑150 (MQ=255)
                                          agaAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGa                                                                                                            >  1:2089430/1‑150 (MQ=255)
                                            aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                          <  2:180839/150‑1 (MQ=255)
                                            aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                          <  2:1813035/150‑1 (MQ=255)
                                            aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                          <  2:1813317/150‑1 (MQ=255)
                                            aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                          <  2:2337557/150‑1 (MQ=255)
                                              aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                        >  1:1070047/1‑150 (MQ=255)
                                              aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                        >  1:1471356/1‑150 (MQ=255)
                                              aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                        >  1:1158840/1‑150 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGACCGTAtt                                                                                                     >  2:1526577/1‑150 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTagt                                                                                                     >  2:1687662/1‑148 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                     >  2:798380/1‑150 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                     >  2:1469099/1‑150 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                     >  2:2253555/1‑150 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                     >  2:562626/1‑150 (MQ=255)
                                                 gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGCGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                     >  2:888237/1‑150 (MQ=255)
                                                  cgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTa                                                                                                    <  1:1746945/150‑1 (MQ=255)
                                                      cAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTc                                                                                                <  1:111564/150‑1 (MQ=255)
                                                              cgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGc                                                                                        <  1:1032022/150‑1 (MQ=255)
                                                                ttACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                      <  1:996108/150‑1 (MQ=255)
                                                                ttACGCCAGAATGCTTTCATAATGGGCGTGGAACTCATACATACACAGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                      <  1:888237/150‑1 (MQ=255)
                                                                   cGCCAGAATGCTTTCATACTGGGTGTGGAACACATACATACACTGAATACTATCTAATAAATCCTAAAACAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                   <  1:1059763/150‑1 (MQ=255)
                                                                   cGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                   <  1:1227847/150‑1 (MQ=255)
                                                                       aGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCgg                                                                               <  1:2304976/150‑1 (MQ=255)
                                                                          aTGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgcca                                                                            <  1:89297/150‑1 (MQ=255)
                                                                           tGCTTTCATACTGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                           <  2:1538551/150‑1 (MQ=255)
                                                                              tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                        >  1:2157589/1‑150 (MQ=255)
                                                                              tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                        >  2:631051/1‑150 (MQ=255)
                                                                              tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                        >  1:1845953/1‑150 (MQ=255)
                                                                               ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                       >  1:1004997/1‑150 (MQ=255)
                                                                               ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                       >  2:1729177/1‑150 (MQ=255)
                                                                                   tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                   >  2:1623905/1‑150 (MQ=255)
                                                                                   tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                   >  2:792262/1‑150 (MQ=255)
                                                                                   tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                   >  2:411200/1‑150 (MQ=255)
                                                                                              gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                        >  1:567606/1‑150 (MQ=255)
                                                                                              gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                        >  1:567582/1‑150 (MQ=255)
                                                                                               aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                       <  1:1126682/150‑1 (MQ=255)
                                                                                               aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                       <  2:2228879/150‑1 (MQ=255)
                                                                                               aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                       <  2:41788/150‑1 (MQ=255)
                                                                                               aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                       <  1:773890/150‑1 (MQ=255)
                                                                                               aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                       <  1:1073567/150‑1 (MQ=255)
                                                                                                  tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                    >  1:549586/1‑150 (MQ=255)
                                                                                                  tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                    <  2:1091289/150‑1 (MQ=255)
                                                                                                  tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                    >  1:1645325/1‑150 (MQ=255)
                                                                                                    atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                  >  1:152309/1‑150 (MQ=255)
                                                                                                    atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                  >  1:152314/1‑150 (MQ=255)
                                                                                                         tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                             >  1:541481/1‑150 (MQ=255)
                                                                                                          acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                            <  1:1558966/150‑1 (MQ=255)
                                                                                                          acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                            >  1:1801814/1‑150 (MQ=255)
                                                                                                          acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                            <  1:1558988/150‑1 (MQ=255)
                                                                                                              tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                        >  2:1701488/1‑150 (MQ=255)
                                                                                                              tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                        >  2:1541775/1‑150 (MQ=255)
                                                                                                              tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                        >  1:1059013/1‑150 (MQ=255)
                                                                                                                     tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                 <  1:1325225/150‑1 (MQ=255)
                                                                                                                       tctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgc                               >  1:172537/1‑150 (MQ=255)
                                                                                                                            tAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCaa                          <  1:1801406/150‑1 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACCTAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGccg                   >  1:1517650/1‑149 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                   >  1:1517658/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                   <  2:152309/150‑1 (MQ=255)
                                                                                                                                   tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                   <  2:152296/150‑1 (MQ=255)
                                                                                                                                     aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                 >  2:1031915/1‑150 (MQ=255)
                                                                                                                                         cgCCCGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATc             >  1:1315784/2‑150 (MQ=255)
                                                                                                                                           cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt           <  1:2074683/150‑1 (MQ=255)
                                                                                                                                           cccGCCAGGTGGGCTTACCGGGCGGGC‑‑‑GTGCTGATTTATTCCGCGTTTGGCGCACGTATTAGGTCCACATGGCGAGAATCGGCCAGCCACCCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt           <  2:2079090/150‑1 (MQ=255)
                                                                                                                                            ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCACCCAACAGCACCATCGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt          >  2:1031249/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt          >  2:180389/1‑150 (MQ=255)
                                                                                                                                            ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt          >  2:1386378/1‑150 (MQ=255)
                                                                                                                                             cGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt         <  1:2188420/150‑1 (MQ=255)
                                                                                                                                                cAGGTTGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt         <  2:1671060/150‑1 (MQ=255)
                                                                                                                                                cAGGTGGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt         <  2:1275300/150‑1 (MQ=255)
                                                                                                                                                  tgTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACCTCGAGATGTAACTCTCCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt    <  2:596522/149‑1 (MQ=255)
                                                                                                                                                  ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt    <  1:742526/150‑1 (MQ=255)
                                                                                                                                                  ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt    <  1:995738/150‑1 (MQ=255)
                                                                                                                                                  ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt    <  1:742301/150‑1 (MQ=255)
                                                                                                                                                   gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc   <  2:1471356/150‑1 (MQ=255)
                                                                                                                                                   gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc   <  2:1158737/150‑1 (MQ=255)
                                                                                                                                                   gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc   <  2:266125/150‑1 (MQ=255)
                                                                                                                                                   gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGACGAGAATCGGCCAGCCAACCAACAGCACCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc   <  2:1070047/150‑1 (MQ=255)
                                                                                                                                                    tGGGCTTACCTGGCGGGC‑‑‑GTGTGGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACGAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAtttga  <  2:668112/150‑3 (MQ=255)
                                                                                                                                                    tGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTca  <  2:390733/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TGCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCA  >  NC_000913/644920‑645217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: