Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 645,070 G→A 5.2% intergenic (‑67/+47) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913645,0700GA 5.2% 538.4 / 11.6 154intergenic (‑67/+47)rna/citTribonuclease I/citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base G (72/74);  new base A (6/2);  total (78/76)
Fisher's exact test for biased strand distribution p-value = 2.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 158 total aligned reads displayed.

GCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACA  >  NC_000913/644921‑645219
                                                                                                                                                     |                                                                                                                                                        
   agCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCt                                                                                                                                                       >  2:1625254/2‑150 (MQ=255)
            aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                              >  2:1558966/1‑150 (MQ=255)
            aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                              >  2:2275173/1‑150 (MQ=255)
            aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                              <  2:6273/150‑1 (MQ=255)
            aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                              <  2:929871/150‑1 (MQ=255)
            aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                              <  2:392807/150‑1 (MQ=255)
               gCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgggc                                                                                                                                           >  2:1801406/1‑150 (MQ=255)
                 gTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCATCGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACTCTGAATACTATCAATTAAATCATAAAATAGCCCGCCAGGTGGGCTTACGTGGGGGGC‑‑‑Gt                                                                                                                                      <  1:1444714/150‑1 (MQ=255)
                     gCAGAAGAGAAGGGAAGCAGAGAAAACGCTAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATAAAAACATAAAAAAGCCCGCCAGGTGGGCTTACATGTCGGCC‑‑‑Gtgatg                                                                                                                                  >  2:1127759/1‑150 (MQ=255)
                      cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtgatga                                                                                                                                 >  1:1091289/1‑150 (MQ=255)
                       agacgagaagGGAAGCAGAGAAGCCGCGAGCAACGCGGCGTTACGCCAGACTGCATTCATAATGGGTGTGGACCTCATACCAACACAGAATCCGCTCGATTAACTCCTCAAAAAGCCCGCCCCGTGGGCTGACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                >  2:1539447/1‑150 (MQ=255)
                       agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                >  1:2100137/1‑150 (MQ=255)
                       agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                >  1:2100210/1‑150 (MQ=255)
                        gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                               >  2:840342/1‑150 (MQ=255)
                        gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                               >  2:866295/1‑150 (MQ=255)
                         aagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAttt                                                                                                                              >  1:266125/1‑150 (MQ=255)
                                      cAGAGAAACCGCGAGCTACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTgg                                                                                                                 <  1:203706/150‑1 (MQ=255)
                                       agagAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGc                                                                                                                >  1:1297185/1‑150 (MQ=255)
                                           aaaCCGCGAGCCACGCGGCGTTACGCCAGAATGCTTTCATACTGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                            <  2:180845/150‑1 (MQ=255)
                                           aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                            <  2:180839/150‑1 (MQ=255)
                                           aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                            <  2:1813317/150‑1 (MQ=255)
                                           aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                            <  2:2337557/150‑1 (MQ=255)
                                             aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                          >  1:1070047/1‑150 (MQ=255)
                                             aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                          >  1:1471356/1‑150 (MQ=255)
                                             aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                          >  1:1158840/1‑150 (MQ=255)
                                                gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                       >  2:798380/1‑150 (MQ=255)
                                                gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                       >  2:1469099/1‑150 (MQ=255)
                                                gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                       >  2:562626/1‑150 (MQ=255)
                                                gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGCGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                       >  2:888237/1‑150 (MQ=255)
                                                 cgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTa                                                                                                      <  1:1746945/150‑1 (MQ=255)
                                                             cgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGc                                                                                          <  1:1032022/150‑1 (MQ=255)
                                                               ttACGCCAGAATGCTTTCATAATGGGCGTGGAACTCATACATACACAGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                        <  1:888237/150‑1 (MQ=255)
                                                                  cGCCAGAATGCTTTCATACTGGGTGTGGAACACATACATACACTGAATACTATCTAATAAATCCTAAAACAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                     <  1:1059763/150‑1 (MQ=255)
                                                                  cGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                     <  1:1227847/150‑1 (MQ=255)
                                                                      aGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCgg                                                                                 <  1:2304976/150‑1 (MQ=255)
                                                                       gAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgc                                                                                <  1:1125145/150‑1 (MQ=255)
                                                                         aTGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgcca                                                                              <  1:89297/150‑1 (MQ=255)
                                                                          tGCTTTCATACTGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                             <  2:1538551/150‑1 (MQ=255)
                                                                          tGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                             <  2:1708765/150‑1 (MQ=255)
                                                                          tGCTTTCATAATGGGTGTGGAACTCATACATAAACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                             <  2:1436583/150‑1 (MQ=255)
                                                                             tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                          >  1:1845953/1‑150 (MQ=255)
                                                                             tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                          >  2:631051/1‑150 (MQ=255)
                                                                              ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                         >  1:1004997/1‑150 (MQ=255)
                                                                              ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                         >  1:181570/1‑150 (MQ=255)
                                                                                  tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                     >  2:1623905/1‑150 (MQ=255)
                                                                                  tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                     >  2:792262/1‑150 (MQ=255)
                                                                                             gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                          >  1:567582/1‑150 (MQ=255)
                                                                                             gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                          >  1:567606/1‑150 (MQ=255)
                                                                                              aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                         <  1:1126682/150‑1 (MQ=255)
                                                                                              aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                         <  1:1975114/150‑1 (MQ=255)
                                                                                              aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                         <  2:41788/150‑1 (MQ=255)
                                                                                              aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                         <  1:773890/150‑1 (MQ=255)
                                                                                              aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                         <  2:2228879/150‑1 (MQ=255)
                                                                                              aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                         <  1:1073567/150‑1 (MQ=255)
                                                                                                 tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                      <  2:1091289/150‑1 (MQ=255)
                                                                                                   atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                    >  1:152296/1‑150 (MQ=255)
                                                                                                   atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                    >  1:152314/1‑150 (MQ=255)
                                                                                                     acatacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTaa                                                  <  1:2355571/150‑1 (MQ=255)
                                                                                                        tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                               >  1:541481/1‑150 (MQ=255)
                                                                                                         acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                              >  1:1801814/1‑150 (MQ=255)
                                                                                                         acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                              >  1:390733/1‑150 (MQ=255)
                                                                                                         acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                              <  1:1558988/150‑1 (MQ=255)
                                                                                                             tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                          >  2:1701488/1‑150 (MQ=255)
                                                                                                             tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                          >  1:1059013/1‑150 (MQ=255)
                                                                                                             tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                          >  1:1756948/1‑150 (MQ=255)
                                                                                                                    tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                   <  1:1325225/150‑1 (MQ=255)
                                                                                                                    tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                   <  1:1371381/150‑1 (MQ=255)
                                                                                                                       ctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgcg                                <  1:1646059/150‑1 (MQ=255)
                                                                                                                       ctagTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgct                                <  2:2252568/150‑2 (MQ=255)
                                                                                                                           tAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCaa                            <  2:1043747/150‑1 (MQ=255)
                                                                                                                                  tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCACCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                     >  1:1517617/1‑150 (MQ=255)
                                                                                                                                  tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                     <  2:152314/150‑1 (MQ=255)
                                                                                                                                  tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                     >  1:1517658/1‑150 (MQ=255)
                                                                                                                                  tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                     >  1:963756/1‑150 (MQ=255)
                                                                                                                                  tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                     <  2:152309/150‑1 (MQ=255)
                                                                                                                                    aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                   >  1:294318/1‑150 (MQ=255)
                                                                                                                                    aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                   >  2:2394938/1‑150 (MQ=255)
                                                                                                                                     aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCACCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTa                  <  2:1983049/150‑1 (MQ=255)
                                                                                                                                     aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTa                  <  2:732189/150‑1 (MQ=255)
                                                                                                                                        cgCCCGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATc               >  1:1554532/2‑150 (MQ=255)
                                                                                                                                        cgCCCGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATc               >  1:1315784/2‑150 (MQ=255)
                                                                                                                                          cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt             <  1:1488117/150‑1 (MQ=255)
                                                                                                                                          cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt             <  1:2074683/150‑1 (MQ=255)
                                                                                                                                          cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt             <  1:2074692/150‑1 (MQ=255)
                                                                                                                                           ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCACCCAACAGCACCATCGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt            >  2:1031249/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTACATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt            >  2:1003095/1‑150 (MQ=255)
                                                                                                                                            cGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt           <  1:2188420/150‑1 (MQ=255)
                                                                                                                                               cAGGTTGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGTTTGCGCCACGACGCCAGTAATCtttt           <  2:1671112/150‑1 (MQ=255)
                                                                                                                                               cAGGTTGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt           <  2:1671060/150‑1 (MQ=255)
                                                                                                                                               cAGGTGGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt           <  2:1520560/150‑1 (MQ=255)
                                                                                                                                               cAGGTGGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt           <  2:1275300/150‑1 (MQ=255)
                                                                                                                                                 tgTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACCTCGAGATGTAACTCTCCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt      <  2:596522/149‑1 (MQ=255)
                                                                                                                                                 ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt      <  1:1605280/150‑1 (MQ=255)
                                                                                                                                                 ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt      <  1:995738/150‑1 (MQ=255)
                                                                                                                                                 ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt      <  1:742526/150‑1 (MQ=255)
                                                                                                                                                 ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt      <  1:742301/150‑1 (MQ=255)
                                                                                                                                                  gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc     <  2:1158840/150‑1 (MQ=255)
                                                                                                                                                   tGGGCTTACCTGGCGGGC‑‑‑GTGTGGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACGAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAtttga    <  2:668112/150‑3 (MQ=255)
                                                                                                                                                   tGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTca    <  2:390733/150‑1 (MQ=255)
                                                                                                                                                     ggCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTcaca  <  1:562626/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
GCCTGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACA  >  NC_000913/644921‑645219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: