Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 645,073 T→C 5.1% intergenic (‑70/+44) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913645,0730TC 5.1% 525.8 / 12.2 157intergenic (‑70/+44)rna/citTribonuclease I/citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base T (70/79);  new base C (6/2);  total (76/81)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 159 total aligned reads displayed.

TGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAT  >  NC_000913/644924‑645220
                                                                                                                                                     |                                                                                                                                                      
agCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCt                                                                                                                                                        >  2:1625254/2‑150 (MQ=255)
   aaCGCTAAGGCGTTGGCAGAATAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTAc                                                                                                                                                     >  1:1827133/1‑150 (MQ=255)
         aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                               <  2:6273/150‑1 (MQ=255)
         aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                               >  2:1558966/1‑150 (MQ=255)
         aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                               >  2:2275173/1‑150 (MQ=255)
         aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                               <  2:929871/150‑1 (MQ=255)
         aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                               <  2:392807/150‑1 (MQ=255)
            gCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgggc                                                                                                                                            >  2:1801406/1‑150 (MQ=255)
              gTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCATCGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACTCTGAATACTATCAATTAAATCATAAAATAGCCCGCCAGGTGGGCTTACGTGGGGGGC‑‑‑Gt                                                                                                                                       <  1:1444714/150‑1 (MQ=255)
               ttGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtg                                                                                                                                      >  1:79648/1‑150 (MQ=255)
                  gCAGAAGAGAAGGGAAGCAGAGAAAACGCTAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATAAAAACATAAAAAAGCCCGCCAGGTGGGCTTACATGTCGGCC‑‑‑Gtgatg                                                                                                                                   >  2:1127759/1‑150 (MQ=255)
                   cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtgatga                                                                                                                                  >  1:1091289/1‑150 (MQ=255)
                    agacgagaagGGAAGCAGAGAAGCCGCGAGCAACGCGGCGTTACGCCAGACTGCATTCATAATGGGTGTGGACCTCATACCAACACAGAATCCGCTCGATTAACTCCTCAAAAAGCCCGCCCCGTGGGCTGACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                 >  2:1539447/1‑150 (MQ=255)
                    agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                 >  1:2100137/1‑150 (MQ=255)
                    agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                 >  1:2100210/1‑150 (MQ=255)
                     gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                                >  2:840342/1‑150 (MQ=255)
                     gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                                >  2:866295/1‑150 (MQ=255)
                      aagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAttt                                                                                                                               >  1:266125/1‑150 (MQ=255)
                        gagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTAt                                                                                                                             <  1:148844/150‑1 (MQ=255)
                                   cAGAGAAACCGCGAGCTACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTgg                                                                                                                  <  1:203706/150‑1 (MQ=255)
                                   cAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTgg                                                                                                                  <  1:203743/150‑1 (MQ=255)
                                    agagAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGc                                                                                                                 >  1:1297185/1‑150 (MQ=255)
                                        aaaCCGCGAGCCACGCGGCGTTACGCCAGAATGCTTTCATACTGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:180845/150‑1 (MQ=255)
                                        aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:180839/150‑1 (MQ=255)
                                        aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:1813317/150‑1 (MQ=255)
                                          aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                           >  1:1471356/1‑150 (MQ=255)
                                          aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                           >  1:1158840/1‑150 (MQ=255)
                                          aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                           >  1:1070047/1‑150 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:1469099/1‑150 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:798380/1‑150 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:476516/1‑150 (MQ=255)
                                             gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGCGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:888237/1‑150 (MQ=255)
                                              cgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTa                                                                                                       <  1:1746945/150‑1 (MQ=255)
                                                          cgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGc                                                                                           <  1:1032022/150‑1 (MQ=255)
                                                            ttACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                         <  2:2313114/150‑1 (MQ=255)
                                                               cGCCAGAATGCTTTCATACTGGGTGTGGAACACATACATACACTGAATACTATCTAATAAATCCTAAAACAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                      <  1:1059763/150‑1 (MQ=255)
                                                               cGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                      <  1:1227847/150‑1 (MQ=255)
                                                                    gAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgc                                                                                 <  1:1125145/150‑1 (MQ=255)
                                                                      aTGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgcca                                                                               <  1:89297/150‑1 (MQ=255)
                                                                       tGCTTTCATACTGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                              <  2:1538551/150‑1 (MQ=255)
                                                                       tGCTTTCATAATGGGTGTGGAACTCATACATAAACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccag                                                                              <  2:1436583/150‑1 (MQ=255)
                                                                          tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                           >  1:1845953/1‑150 (MQ=255)
                                                                          tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                           >  2:631051/1‑150 (MQ=255)
                                                                           ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                          >  1:1004997/1‑150 (MQ=255)
                                                                           ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                          >  2:1729177/1‑150 (MQ=255)
                                                                               tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                      >  2:1623905/1‑150 (MQ=255)
                                                                               tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                      >  2:792262/1‑150 (MQ=255)
                                                                                          gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                           >  1:567606/1‑150 (MQ=255)
                                                                                          gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                           >  1:567413/1‑150 (MQ=255)
                                                                                           aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  1:1126682/150‑1 (MQ=255)
                                                                                           aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  1:773890/150‑1 (MQ=255)
                                                                                           aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  2:2228879/150‑1 (MQ=255)
                                                                                           aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  1:1073567/150‑1 (MQ=255)
                                                                                           aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  2:41788/150‑1 (MQ=255)
                                                                                              tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                       >  1:1645325/1‑150 (MQ=255)
                                                                                              tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                       <  2:1091289/150‑1 (MQ=255)
                                                                                                atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                     >  1:152314/1‑150 (MQ=255)
                                                                                                atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                     >  1:152296/1‑150 (MQ=255)
                                                                                                  acatacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTaa                                                   <  1:2355571/150‑1 (MQ=255)
                                                                                                     tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                                >  1:541481/1‑150 (MQ=255)
                                                                                                      acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               >  1:390733/1‑150 (MQ=255)
                                                                                                      acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               >  1:1801814/1‑150 (MQ=255)
                                                                                                      acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               <  1:1558988/150‑1 (MQ=255)
                                                                                                      acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               <  1:2275173/150‑1 (MQ=255)
                                                                                                          tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  1:1756948/1‑150 (MQ=255)
                                                                                                          tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  2:1701488/1‑150 (MQ=255)
                                                                                                          tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  1:1059013/1‑150 (MQ=255)
                                                                                                                 tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                    <  1:1371381/150‑1 (MQ=255)
                                                                                                                 tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                    <  1:1325225/150‑1 (MQ=255)
                                                                                                                    ctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgcg                                 <  1:1646059/150‑1 (MQ=255)
                                                                                                                    ctagTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgct                                 <  2:2252568/150‑2 (MQ=255)
                                                                                                                        tAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCaa                             <  2:1043747/150‑1 (MQ=255)
                                                                                                                               tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCACCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      >  1:1517617/1‑150 (MQ=255)
                                                                                                                               tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      <  2:152314/150‑1 (MQ=255)
                                                                                                                               tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      <  1:1324383/150‑1 (MQ=255)
                                                                                                                               tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      <  2:152309/150‑1 (MQ=255)
                                                                                                                               tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      >  1:1517658/1‑150 (MQ=255)
                                                                                                                               tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      >  1:963756/1‑150 (MQ=255)
                                                                                                                                 aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                    >  1:294318/1‑150 (MQ=255)
                                                                                                                                 aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                    >  2:2394938/1‑150 (MQ=255)
                                                                                                                                  aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTa                   <  2:732189/150‑1 (MQ=255)
                                                                                                                                     cgCCCGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATc                >  1:1554532/2‑150 (MQ=255)
                                                                                                                                       cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt              <  1:2074692/150‑1 (MQ=255)
                                                                                                                                       cccGCCAGGTGGGCTTACCGGGCGGGC‑‑‑GTGCTGATTTATTCCGCGTTTGGCGCACGTATTAGGTCCACATGGCGAGAATCGGCCAGCCACCCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt              <  2:2079090/150‑1 (MQ=255)
                                                                                                                                        ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCACCCAACAGCACCATCGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt             >  2:1031249/1‑150 (MQ=255)
                                                                                                                                        ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTACATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt             >  2:1003095/1‑150 (MQ=255)
                                                                                                                                         cGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt            <  1:2188420/150‑1 (MQ=255)
                                                                                                                                            cAGGTTGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGTTTGCGCCACGACGCCAGTAATCtttt            <  2:1671112/150‑1 (MQ=255)
                                                                                                                                            cAGGTTGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt            <  2:1671060/150‑1 (MQ=255)
                                                                                                                                            cAGGTGGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt            <  2:1275300/150‑1 (MQ=255)
                                                                                                                                            cAGGTGGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt            <  2:1520560/150‑1 (MQ=255)
                                                                                                                                              tgTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACCTCGAGATGTAACTCTCCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  2:596522/149‑1 (MQ=255)
                                                                                                                                              ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  1:995738/150‑1 (MQ=255)
                                                                                                                                              ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  1:742526/150‑1 (MQ=255)
                                                                                                                                              ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  1:742301/150‑1 (MQ=255)
                                                                                                                                               gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc      <  2:1158840/150‑1 (MQ=255)
                                                                                                                                                tGGGCTTACCTGGCGGGC‑‑‑GTGTGGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACGAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAtttga     <  2:668112/150‑3 (MQ=255)
                                                                                                                                                tGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTca     <  2:390733/150‑1 (MQ=255)
                                                                                                                                                  ggCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTcaca   <  1:562626/150‑1 (MQ=255)
                                                                                                                                                   gCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAt  <  2:2100137/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TGCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAT  >  NC_000913/644924‑645220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: