Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 645,074 T→C 5.1% intergenic (‑71/+43) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913645,0740TC 5.1% 517.8 / 12.5 157intergenic (‑71/+43)rna/citTribonuclease I/citrate/succinate antiporter; citrate carrier
Reads supporting (aligned to +/- strand):  ref base T (69/79);  new base C (6/2);  total (76/81)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01

Only 100 of 158 total aligned reads displayed.

GCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAT  >  NC_000913/644925‑645220
                                                                                                                                                     |                                                                                                                                                     
    cGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCt                                                                                                                                                   <  1:303780/150‑1 (MQ=255)
        aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                               >  2:1558988/1‑150 (MQ=255)
        aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                               >  2:2275173/1‑150 (MQ=255)
        aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcg                                                                                                                                               <  2:6273/150‑1 (MQ=255)
        aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                               <  2:929871/150‑1 (MQ=255)
        aaGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTAAGCCCACCTggcg                                                                                                                                               <  2:392807/150‑1 (MQ=255)
           gCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTggcgggc                                                                                                                                            >  2:1801406/1‑150 (MQ=255)
             gTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCATCGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACTCTGAATACTATCAATTAAATCATAAAATAGCCCGCCAGGTGGGCTTACGTGGGGGGC‑‑‑Gt                                                                                                                                       <  1:1444714/150‑1 (MQ=255)
              ttGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtg                                                                                                                                      >  1:79648/1‑150 (MQ=255)
                ggCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtgat                                                                                                                                    >  2:1661994/1‑150 (MQ=255)
                 gCAGAAGAGAAGGGAAGCAGAGAAAACGCTAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATAAAAACATAAAAAAGCCCGCCAGGTGGGCTTACATGTCGGCC‑‑‑Gtgatg                                                                                                                                   >  2:1127759/1‑150 (MQ=255)
                  cAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑Gtgatga                                                                                                                                  >  1:1091289/1‑150 (MQ=255)
                   agacgagaagGGAAGCAGAGAAGCCGCGAGCAACGCGGCGTTACGCCAGACTGCATTCATAATGGGTGTGGACCTCATACCAACACAGAATCCGCTCGATTAACTCCTCAAAAAGCCCGCCCCGTGGGCTGACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                 >  2:1539447/1‑150 (MQ=255)
                   agaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTgatgat                                                                                                                                 >  1:2100210/1‑150 (MQ=255)
                    gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                                >  2:840342/1‑150 (MQ=255)
                    gaagagaagGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGAtt                                                                                                                                >  2:866295/1‑150 (MQ=255)
                                  cAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTgg                                                                                                                  <  1:203743/150‑1 (MQ=255)
                                   agagAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGc                                                                                                                 >  1:1297185/1‑150 (MQ=255)
                                     agaAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGa                                                                                                               >  1:2395543/1‑150 (MQ=255)
                                     agaAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGa                                                                                                               >  1:2089430/1‑150 (MQ=255)
                                       aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:180839/150‑1 (MQ=255)
                                       aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:1813035/150‑1 (MQ=255)
                                       aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:1813317/150‑1 (MQ=255)
                                       aaaCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAAc                                                                                                             <  2:2337557/150‑1 (MQ=255)
                                         aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                           >  1:1471356/1‑150 (MQ=255)
                                         aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                           >  1:1158840/1‑150 (MQ=255)
                                         aCCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGt                                                                                                           >  1:1070047/1‑150 (MQ=255)
                                            gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGACCGTAtt                                                                                                        >  2:1526577/1‑150 (MQ=255)
                                            gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTagt                                                                                                        >  2:1687662/1‑148 (MQ=255)
                                            gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:1469099/1‑150 (MQ=255)
                                            gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:562626/1‑150 (MQ=255)
                                            gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:798380/1‑150 (MQ=255)
                                            gcgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGCGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTAtt                                                                                                        >  2:888237/1‑150 (MQ=255)
                                             cgAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTa                                                                                                       <  1:1746945/150‑1 (MQ=255)
                                                         cgTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGc                                                                                           <  1:1032022/150‑1 (MQ=255)
                                                           ttACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                         <  1:996108/150‑1 (MQ=255)
                                                           ttACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCga                                                                                         <  1:61972/150‑1 (MQ=255)
                                                              cGCCAGAATGCTTTCATACTGGGTGTGGAACACATACATACACTGAATACTATCTAATAAATCCTAAAACAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                      <  1:1059763/150‑1 (MQ=255)
                                                              cGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGaa                                                                                      <  1:1227847/150‑1 (MQ=255)
                                                                   gAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgc                                                                                 <  1:1125145/150‑1 (MQ=255)
                                                                     aTGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgcca                                                                               <  1:89297/150‑1 (MQ=255)
                                                                         tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                           >  1:2157589/1‑150 (MQ=255)
                                                                         tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                           >  2:631051/1‑150 (MQ=255)
                                                                         tttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGgccagcca                                                                           >  1:1845953/1‑150 (MQ=255)
                                                                          ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                          >  1:1004997/1‑150 (MQ=255)
                                                                          ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                          >  2:1729177/1‑150 (MQ=255)
                                                                          ttCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaa                                                                          >  1:181570/1‑150 (MQ=255)
                                                                              tAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGccaaccaa                                                                      >  2:792262/1‑150 (MQ=255)
                                                                                         gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                           >  1:567606/1‑150 (MQ=255)
                                                                                         gAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATag                                                           >  1:567413/1‑150 (MQ=255)
                                                                                          aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  1:1126682/150‑1 (MQ=255)
                                                                                          aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  2:2228879/150‑1 (MQ=255)
                                                                                          aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  1:1073567/150‑1 (MQ=255)
                                                                                          aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  2:41788/150‑1 (MQ=255)
                                                                                          aaCTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATaga                                                          <  1:1975114/150‑1 (MQ=255)
                                                                                             tCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGAt                                                       >  1:549586/1‑150 (MQ=255)
                                                                                               atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                     >  1:152314/1‑150 (MQ=255)
                                                                                               atacatacACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGt                                                     >  1:152296/1‑150 (MQ=255)
                                                                                                 acatacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTaa                                                   <  1:2355571/150‑1 (MQ=255)
                                                                                                    tacaCTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATc                                                >  1:541481/1‑150 (MQ=255)
                                                                                                     acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               >  1:1801814/1‑150 (MQ=255)
                                                                                                     acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               <  1:2275173/150‑1 (MQ=255)
                                                                                                     acacTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCa                                               <  1:1558988/150‑1 (MQ=255)
                                                                                                         tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  1:1756948/1‑150 (MQ=255)
                                                                                                         tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  1:1059013/1‑150 (MQ=255)
                                                                                                         tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  2:1701488/1‑150 (MQ=255)
                                                                                                         tGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCAcccc                                           >  2:1541775/1‑150 (MQ=255)
                                                                                                                tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                    <  1:1325225/150‑1 (MQ=255)
                                                                                                                tatctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGAtt                                    <  1:1371381/150‑1 (MQ=255)
                                                                                                                   ctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgcg                                 <  1:747820/150‑1 (MQ=255)
                                                                                                                   ctatTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgcg                                 <  1:1646059/150‑1 (MQ=255)
                                                                                                                   ctagTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTgct                                 <  2:2252568/150‑2 (MQ=255)
                                                                                                                              tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCACCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      >  1:1517617/1‑150 (MQ=255)
                                                                                                                              tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      <  2:152314/150‑1 (MQ=255)
                                                                                                                              tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      >  1:1517658/1‑150 (MQ=255)
                                                                                                                              tAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCa                      <  2:152296/150‑1 (MQ=255)
                                                                                                                                aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                    >  1:294318/1‑150 (MQ=255)
                                                                                                                                aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                    >  2:1031915/1‑150 (MQ=255)
                                                                                                                                aaaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGt                    >  2:2394938/1‑150 (MQ=255)
                                                                                                                                 aaaaGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTa                   <  2:732189/150‑1 (MQ=255)
                                                                                                                                    cgCCCGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATc                >  1:1554532/2‑150 (MQ=255)
                                                                                                                                    cgCCCGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATc                >  1:1315784/2‑150 (MQ=255)
                                                                                                                                      cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt              <  1:2074692/150‑1 (MQ=255)
                                                                                                                                      cccGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt              <  1:1488117/150‑1 (MQ=255)
                                                                                                                                      cccGCCAGGTGGGCTTACCGGGCGGGC‑‑‑GTGCTGATTTATTCCGCGTTTGGCGCACGTATTAGGTCCACATGGCGAGAATCGGCCAGCCACCCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtt              <  2:2079090/150‑1 (MQ=255)
                                                                                                                                       ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGACTCGGCCAGCCACCCAACAGCACCATCGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt             >  2:1031249/1‑150 (MQ=255)
                                                                                                                                       ccGCCAGGTAAGCCCACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCttt             >  2:1386378/1‑150 (MQ=255)
                                                                                                                                           cAGGTTGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt            <  2:1671060/150‑1 (MQ=255)
                                                                                                                                           cAGGTGGGCTTACCTGGCGGGCTTTTTTATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCtttt            <  2:1275300/150‑1 (MQ=255)
                                                                                                                                             tgTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCCGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACCTCGAGATGTAACTCTCCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  2:596522/149‑1 (MQ=255)
                                                                                                                                             ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  1:742526/150‑1 (MQ=255)
                                                                                                                                             ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  1:995738/150‑1 (MQ=255)
                                                                                                                                             ggTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAttt       <  1:1605280/150‑1 (MQ=255)
                                                                                                                                              gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc      <  2:1471356/150‑1 (MQ=255)
                                                                                                                                              gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc      <  2:1158737/150‑1 (MQ=255)
                                                                                                                                              gTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGACGAGAATCGGCCAGCCAACCAACAGCACCATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTc      <  2:1070047/150‑1 (MQ=255)
                                                                                                                                               tGGGCTTACCTGGCGGGC‑‑‑GTGTGGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACGAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGAtttga     <  2:668112/150‑3 (MQ=255)
                                                                                                                                               tGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTca     <  2:390733/150‑1 (MQ=255)
                                                                                                                                                 ggCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTcaca   <  1:960566/150‑1 (MQ=255)
                                                                                                                                                  gCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAt  <  2:2100137/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAACGCTAAGGCGTTGGCAGAAGAGAAGGGAAGCAGAGAAACCGCGAGCAACGCGGCGTTACGCCAGAATGCTTTCATAATGGGTGTGGAACTCATACATACACTGAATACTATCTATTAAATCATAAAAAAGCCCGCCAGGTGGGCTTACCTGGCGGGC‑‑‑GTGATGATTTATTCAGCGTTTGGCGAACGTATTAGTTCCACATGGCGAGAATCGGCCAGCCAACCAACAGCAACATAGAGATGTAAATCACCCCGAAGATTGCGCCAAGACGCCAGTAATCTTTTGATTTCACAT  >  NC_000913/644925‑645220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: