Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 760,167 G→A 5.8% A488T (GCT→ACT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913760,1670GA 5.8% 474.9 / 11.6 140A488T (GCT→ACT) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (60/71);  new base A (4/4);  total (64/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

GATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTC  >  NC_000913/760020‑760316
                                                                                                                                                   |                                                                                                                                                     
         aaCACCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTg                                                                                                                                            <  2:913301/150‑1 (MQ=255)
         aaCACCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTg                                                                                                                                            <  1:1169832/150‑1 (MQ=255)
         aaCACCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTg                                                                                                                                            <  1:2020708/150‑1 (MQ=255)
         aaCACCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTg                                                                                                                                            <  2:2280230/150‑1 (MQ=255)
           cacCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGa                                                                                                                                          <  2:2281028/150‑1 (MQ=255)
              cTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCa                                                                                                                                       >  2:1125850/1‑150 (MQ=255)
              cTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCa                                                                                                                                       >  2:647588/1‑150 (MQ=255)
              cTTTAAACGTGATGTCTTCATCGACCTGGTGTGCGACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCa                                                                                                                                       <  2:1380184/150‑1 (MQ=255)
               tttAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAg                                                                                                                                      >  1:1949171/1‑150 (MQ=255)
               tttAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGAAGTATCAGAAAATCAAAAAACAACCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAg                                                                                                                                      >  1:2415110/1‑150 (MQ=255)
                ttAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAgg                                                                                                                                     <  1:259444/150‑1 (MQ=255)
                         aTGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCTGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTgg                                                                                                                            <  1:1611746/150‑1 (MQ=255)
                         aTGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTgg                                                                                                                            >  2:120080/1‑150 (MQ=255)
                         aTGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTgg                                                                                                                            >  2:232418/1‑150 (MQ=255)
                          tGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGc                                                                                                                           <  2:2231389/150‑1 (MQ=255)
                          tGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGc                                                                                                                           <  2:184794/150‑1 (MQ=255)
                          tGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGc                                                                                                                           <  2:707178/150‑1 (MQ=255)
                             cTTCATCGACCTGGTGTGCTACCGCGGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAGAAAGTGGCGAc                                                                                                                        >  2:1448919/1‑150 (MQ=255)
                             cTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGAc                                                                                                                        >  2:1448881/1‑150 (MQ=255)
                              ttCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACg                                                                                                                       >  1:568422/1‑150 (MQ=255)
                              ttCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACg                                                                                                                       >  2:1201978/1‑150 (MQ=255)
                                 aTCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGGTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTg                                                                                                                    >  1:642888/1‑150 (MQ=255)
                                 aTCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTg                                                                                                                    >  1:1673063/1‑150 (MQ=255)
                                  tCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAACGTGGCGACGCTgg                                                                                                                   >  2:1533864/1‑150 (MQ=255)
                                  tCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTgg                                                                                                                   >  2:136336/1‑150 (MQ=255)
                                      ccTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGa                                                                                                               <  2:1543883/150‑1 (MQ=255)
                                         ggTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGc                                                                                                            <  1:223282/150‑1 (MQ=255)
                                         ggTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGc                                                                                                            <  1:223290/150‑1 (MQ=255)
                                                 aCCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCgaga                                                                                                    <  1:2064336/150‑1 (MQ=255)
                                                 aCCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCgaga                                                                                                    <  2:1183635/150‑1 (MQ=255)
                                                  ccgccgTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGAt                                                                                                   >  1:726473/1‑150 (MQ=255)
                                                  ccgccgTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCAGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGAt                                                                                                   >  1:444148/1‑150 (MQ=255)
                                                  ccgccgTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACAACCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGAt                                                                                                   >  1:2282119/1‑150 (MQ=255)
                                                   cgccgTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATg                                                                                                  >  1:340657/1‑150 (MQ=255)
                                                         cACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAAc                                                                                            <  2:1149380/150‑1 (MQ=255)
                                                            ggCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTg                                                                                         >  1:286984/1‑150 (MQ=255)
                                                            ggCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTg                                                                                         <  1:207009/150‑1 (MQ=255)
                                                             gCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGt                                                                                        >  2:932879/1‑150 (MQ=255)
                                                                  aaCGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACACTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACcgc                                                                                   >  2:1113651/1‑150 (MQ=255)
                                                                  aaCGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACACTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACcgc                                                                                   >  2:1028107/1‑150 (MQ=255)
                                                                   aCGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACcgcg                                                                                  >  1:751848/1‑150 (MQ=255)
                                                                     gAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGAt                                                                                <  2:1266948/150‑1 (MQ=255)
                                                                       aGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATgc                                                                              >  1:2276182/1‑150 (MQ=255)
                                                                       aGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGGATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATgc                                                                              >  1:600167/1‑150 (MQ=255)
                                                                         ccGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATgcgc                                                                            <  1:1127105/150‑1 (MQ=255)
                                                                           gacgaGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTg                                                                          <  1:1245333/150‑1 (MQ=255)
                                                                           gacgaGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTg                                                                          <  1:905252/150‑1 (MQ=255)
                                                                             cgagccgagcGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGa                                                                        <  1:1225510/150‑1 (MQ=255)
                                                                             cgagccgagcGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGa                                                                        <  1:2125541/150‑1 (MQ=255)
                                                                                   gagcGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTgg                                                                  >  2:682330/1‑150 (MQ=255)
                                                                                   gagcGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTgg                                                                  >  2:1327406/1‑150 (MQ=255)
                                                                                   gagcGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTgg                                                                  <  2:1090794/150‑1 (MQ=255)
                                                                                   gagcGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTgg                                                                  >  2:1887519/1‑150 (MQ=255)
                                                                                      cgcAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGa                                                               <  1:54448/150‑1 (MQ=255)
                                                                                      cgcAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGa                                                               >  2:232116/1‑150 (MQ=255)
                                                                                         aaCCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTg                                                            <  1:1464770/150‑1 (MQ=255)
                                                                                         aaCCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTg                                                            <  1:321262/150‑1 (MQ=255)
                                                                                          aCCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGc                                                           <  2:462548/150‑1 (MQ=255)
                                                                                          aCCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGc                                                           <  1:996826/150‑1 (MQ=255)
                                                                                          aCCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGc                                                           <  1:1833665/150‑1 (MQ=255)
                                                                                            ccAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGAAGGAAAAAGTGGCGACGCTGGAATATGACACCGAGATGGTTAAAATGAACAGCGAAGCGATGTATGATGgagaattctt                                                         >  2:1304041/1‑144 (MQ=255)
                                                                                              aGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAg                                                       >  2:551236/1‑150 (MQ=255)
                                                                                              aGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAg                                                       >  1:1465059/1‑150 (MQ=255)
                                                                                              aGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAg                                                       >  2:229278/1‑150 (MQ=255)
                                                                                               gCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      >  1:796886/1‑150 (MQ=255)
                                                                                               gCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      >  1:796876/1‑150 (MQ=255)
                                                                                               gCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      <  2:713980/150‑1 (MQ=255)
                                                                                               gCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      <  2:2219504/150‑1 (MQ=255)
                                                                                               gCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      <  2:2219413/150‑1 (MQ=255)
                                                                                               gCCGCTGATGTATCAGAAAAACAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      <  2:199767/150‑1 (MQ=255)
                                                                                               gCCGCTCAACTATCAGAAAATCAAAAAACATCCCACACCCCGCAAAATCTACCCTGACAACCTGGAGCACGAAAAAGTGGCGACGCAGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGt                                                      <  2:199769/150‑1 (MQ=255)
                                                                                                ccGCTGATGTATCAGAACATCAAAAAACATCCGACACCGCGCAAAAACTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGTGTAGTg                                                     <  1:1511124/150‑1 (MQ=255)
                                                                                                ccGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGTGTAGTg                                                     <  1:599086/150‑1 (MQ=255)
                                                                                                ccGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTg                                                     <  1:1366001/150‑1 (MQ=255)
                                                                                                ccGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTg                                                     <  1:209327/150‑1 (MQ=255)
                                                                                                            cAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTAAGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTAAACCTGTACCCCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGt                                         >  2:47896/1‑150 (MQ=255)
                                                                                                                aaaTCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGa                                     <  2:1658918/150‑1 (MQ=255)
                                                                                                                aaaTCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGa                                     <  2:1658931/150‑1 (MQ=255)
                                                                                                                aaaTCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGa                                     <  1:294213/150‑1 (MQ=255)
                                                                                                                    cAAAAAACATCCGACACCGCGCAAAATCTACACTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGaa                                 <  1:1028107/150‑1 (MQ=255)
                                                                                                                    cAAAAAACATCCGACACCGCGCAAAATCTACACTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGaa                                 <  1:1028161/150‑1 (MQ=255)
                                                                                                                     aaaaaaCATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAAc                                >  1:1702093/1‑150 (MQ=255)
                                                                                                                     aaaaaaCATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAAc                                >  1:102788/1‑150 (MQ=255)
                                                                                                                      aaaaaCATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGATAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACa                               >  2:1841726/1‑150 (MQ=255)
                                                                                                                               cGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCtt                      >  1:474360/1‑150 (MQ=255)
                                                                                                                                gACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCttt                     >  1:491255/1‑150 (MQ=255)
                                                                                                                                gACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCttt                     <  2:2415110/150‑1 (MQ=255)
                                                                                                                                 acacCGCGCAAACTCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTc                    <  2:141776/150‑1 (MQ=255)
                                                                                                                                 acacCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTc                    <  2:1469393/150‑1 (MQ=255)
                                                                                                                                 acacCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTc                    <  2:1541626/150‑1 (MQ=255)
                                                                                                                                 acacCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTc                    <  2:243839/150‑1 (MQ=255)
                                                                                                                                 acacCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTc                    <  2:1370574/150‑1 (MQ=255)
                                                                                                                                   acCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCAc                  <  2:2126888/150‑1 (MQ=255)
                                                                                                                                       cgcAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTgg              >  1:1272254/1‑150 (MQ=255)
                                                                                                                                           aaaTCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGc          <  2:1416362/150‑1 (MQ=255)
                                                                                                                                           aaaTCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGc          <  1:2343037/150‑1 (MQ=255)
                                                                                                                                             aTCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCg        >  1:1655542/1‑150 (MQ=255)
                                                                                                                                                   gCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTc  >  1:417571/1‑150 (MQ=255)
                                                                                                                                                   gCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTc  >  1:417529/1‑150 (MQ=255)
                                                                                                                                                   gCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGAACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTc  >  1:1421144/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGACCTGGTGTGCTACCGCCGTCACGGCCACAACGAAGCCGACGAGCCGAGCGCAACCCAGCCGCTGATGTATCAGAAAATCAAAAAACATCCGACACCGCGCAAAATCTACGCTGACAAGCTGGAGCAGGAAAAAGTGGCGACGCTGGAAGATGCCACCGAGATGGTTAACCTGTACCGCGATGCGCTGGATGCTGGCGATTGCGTAGTGGCAGAGTGGCGTCCGATGAACATGCACTCTTTCACCTGGTCGCCGTACCTC  >  NC_000913/760020‑760316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: