Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 838,930 T→A 8.5% Y93F (TAT→TTT)  ybiX ← Fe(II)‑dependent oxygenase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913838,9300TA 8.5% 482.6 / 9.9 154Y93F (TAT→TTT) ybiXFe(II)‑dependent oxygenase superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (65/70);  new base A (15/3);  total (81/73)
Fisher's exact test for biased strand distribution p-value = 5.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

TACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTT  >  NC_000913/838782‑839077
                                                                                                                                                    |                                                                                                                                                   
 aCCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACTATAg                                                                                                                                                   <  1:1851157/150‑1 (MQ=255)
 aCCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAg                                                                                                                                                   <  1:1314941/150‑1 (MQ=255)
   ccGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGt                                                                                                                                                 >  2:1643241/1‑150 (MQ=255)
        gttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCTATGGTTTcca                                                                                                                                            <  1:1755701/150‑3 (MQ=255)
        gttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCTATGGTTTcca                                                                                                                                            <  1:886651/150‑3 (MQ=255)
         ttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCAtt                                                                                                                                           >  2:376105/1‑150 (MQ=255)
         ttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCAtt                                                                                                                                           >  1:1800977/1‑150 (MQ=255)
         ttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCAAAGGTTTCAtt                                                                                                                                           >  1:754096/1‑150 (MQ=255)
         ttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACAAGGAAACCATAGGTTTCAtt                                                                                                                                           >  2:1088431/1‑150 (MQ=255)
         ttgtCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCACCATCCACATGGAAACCATAGGTTTCAtt                                                                                                                                           >  2:1471292/1‑150 (MQ=255)
            tCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGtt                                                                                                                                        <  1:1178937/150‑1 (MQ=255)
            tCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGtt                                                                                                                                        <  1:972074/150‑1 (MQ=255)
            tCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCAAACACAAGGAAACCATAGGTTTCAATGtt                                                                                                                                        >  1:1034421/1‑150 (MQ=255)
             ccGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTc                                                                                                                                       <  1:1079866/150‑1 (MQ=255)
               gAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTg                                                                                                                                     <  2:2158808/150‑1 (MQ=255)
                   gtgtCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAg                                                                                                                                 <  2:1804322/150‑1 (MQ=255)
                   gtgtCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAg                                                                                                                                 <  1:2170907/150‑1 (MQ=255)
                      tCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGa                                                                                                                              <  2:1105395/150‑1 (MQ=255)
                           aaCGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTaaa                                                                                                                         <  1:679022/150‑1 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGTTGGCTGCGTTCAGCGCCATCCACATGGAAACCAAAGGTTTAATTTTTCTGATAGCGATTAAACa                                                                                                                       >  1:2218753/1‑150 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGTTGGCTGCGTACTGCGCCAACCACAAGGAAACCATGTGTTTCATTGTTGTGGTAGCGCTTTAACa                                                                                                                       >  1:2219001/1‑150 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGTTGGCTGCGTAATGCGCCATCCACAAGGAAACCATAGGTTTAATTGTTCTGATAGCGATTAAACa                                                                                                                       >  1:865604/1‑150 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACa                                                                                                                       <  2:2138733/150‑1 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACa                                                                                                                       >  1:55700/1‑150 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTAATTGTTCTGTTAGCGTTTAAACa                                                                                                                       >  1:1636870/1‑150 (MQ=255)
                             cGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACAAGGAAACCCTAGGTTTCATTGTTATGATAGCGATTAAACa                                                                                                                       >  1:1636834/1‑150 (MQ=255)
                              gACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAg                                                                                                                      <  1:1985816/150‑1 (MQ=255)
                              gACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACAAGGAAACCAAAGGTTTCATTGTTCTGATAGCGATTAAACAg                                                                                                                      >  1:1602046/1‑150 (MQ=255)
                              gACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCAACCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAg                                                                                                                      >  1:1601964/1‑150 (MQ=255)
                                  aGTTCGCCGCCGTCGTATCTTTGTGGATCGCTTAAAAACAGCGTTGCAGAATGTTCAGTACGCATCCAGACGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTATGATAGCGATTAAACAgcggc                                                                                                                  <  2:85117/150‑1 (MQ=255)
                                  aGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGGTGGCTGCGTACAGCGCCAACCACAAGGAAAACAAAGGTTTGATTTTTGTGATAGCGATTAAAAAgcggc                                                                                                                  >  1:422121/1‑150 (MQ=255)
                                  aGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACAAGGAAACCAAAGGTTTCATTGTTCTGATATCGATTAAACAgcggc                                                                                                                  >  1:943790/1‑150 (MQ=255)
                                  aGTTCGCCGCCGTCGTAGCTTTGTGGATCGATTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGTAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAgcggc                                                                                                                  <  1:630795/150‑1 (MQ=255)
                                     tCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTTTGGTTGGGTGGGTTATTGGCCATACACATTGAAAACATATGTTTGATTTTTGTGTTTGAGATTAAAAATCGGCGTg                                                                                                               >  1:1844420/1‑150 (MQ=255)
                                     tCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTTTGGATGGCTGCGTAATGCGCCATCCACAAGGAAACCAAAGGTTTGATTTTTGTGATAGCGATTAAAAATCGGCGTg                                                                                                               >  1:1844412/1‑150 (MQ=255)
                                     tCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTg                                                                                                               <  2:1802605/150‑1 (MQ=255)
                                     tCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTg                                                                                                               <  2:927673/150‑1 (MQ=255)
                                          gccgTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGACAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCAACCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAg                                                                                                          >  2:747920/1‑150 (MQ=255)
                                            cgtcgtAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAggg                                                                                                        <  1:743553/150‑1 (MQ=255)
                                             gtcgtAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCCTACTGCGCCAACCACAAGGAAACCAAAGGTTTCAATGTTCTGATAGCGATTAAACAGCGGCGGGGCAAGGGt                                                                                                       >  1:1239521/1‑150 (MQ=255)
                                              tcgtAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTa                                                                                                      >  2:470931/1‑150 (MQ=255)
                                                 tAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTAcgc                                                                                                   >  2:891355/1‑150 (MQ=255)
                                                      ttGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTAATGCGCCAACCACAAGGAAAACAAAGGTTTAATTTTTGTGGTATCGATTAAACAGCGGCGTGGGGAGGGTGAGCGGCac                                                                                              >  1:1158923/1‑149 (MQ=255)
                                                            aTCTCTTAAAAACAGCTTGTAAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCAATCAAAATGGAAACCATAGTTTTAATTGTTCTTATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCcgc                                                                                        <  1:2037800/150‑1 (MQ=255)
                                                            aTCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTTTGGGTGGGTGGGTACAGCGCCAACCACAATGAAACCAAAGGGTTGATTGTTGTGTTAGAGGGTTAAAAGCGGCGTGGGAAGGGGACGCGGCCAGGCcgc                                                                                        >  1:923425/1‑150 (MQ=255)
                                                            aTCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGTAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCcgc                                                                                        <  1:1284025/150‑1 (MQ=255)
                                                            aTCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCcgc                                                                                        >  2:2191691/1‑150 (MQ=255)
                                                            aTCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCcgc                                                                                        >  2:2019764/1‑150 (MQ=255)
                                                            aTCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCcgc                                                                                        >  2:778395/1‑150 (MQ=255)
                                                            aTCGCTTAAAAACAGCGTGGCAGAAAGGACAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCAACCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTGAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCcgc                                                                                        >  2:1149646/1‑150 (MQ=255)
                                                              cGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCgg                                                                                      <  1:1403611/150‑1 (MQ=255)
                                                               gCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGGTGGCTGCGTACAGCGCCATCCACAAGGAAACCAAAGGTTTAAATTTTGTTATAGCGATTAAAAAGCGGCGTGGCAAGGGTAAGCGGCAAGGCcgccgc                                                                                     >  1:2193759/1‑150 (MQ=255)
                                                               gCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCAACCACAAGGAAACCAAAGGTTTCATTTTTATGATATCGATTAAACAGAGGCGTGGAAATGGTACGCCGCCAGGCCGCGGc                                                                                     >  1:1786688/1‑150 (MQ=255)
                                                                     aaaCAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGaa                                                                               >  2:1201181/1‑150 (MQ=255)
                                                                         aGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATaaa                                                                           <  1:1980059/150‑1 (MQ=255)
                                                                         aGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATaaa                                                                           <  2:1872013/150‑1 (MQ=255)
                                                                         aGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATaaa                                                                           <  1:1522423/150‑1 (MQ=255)
                                                                          gCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAg                                                                          <  1:114744/150‑1 (MQ=255)
                                                                           cGTGGCAGAAAGGTCAGTACGCATCCAGCCGTGTTGTGGGTGGCGGCGTACAGCCCCAACCACAAGGAAACCCAACGGTTCAATGTTCTGGTAGCGAGTAAACACCCGCCGGGGCAGGGGACGCGGCCCCGCCCCGGCCAACAAAAAAGc                                                                         >  1:1263838/1‑150 (MQ=255)
                                                                            gTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCa                                                                        >  2:177242/1‑150 (MQ=255)
                                                                                       gTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAAc                                                             >  2:1530930/1‑150 (MQ=255)
                                                                                          aGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACcgc                                                          <  1:43011/150‑1 (MQ=255)
                                                                                          aGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACcgc                                                          <  1:1262687/150‑1 (MQ=255)
                                                                                          aGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACcgc                                                          <  1:1302713/150‑1 (MQ=255)
                                                                                          aGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCCATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACcgc                                                          <  1:2022865/150‑1 (MQ=255)
                                                                                             tcgaaTCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATACACATTGAAACCATAGGTTTCTTTGTTCTGTTAGCGATTTAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGTCAAAGAATAAAGCATGTTGGTTAACCGCGtt                                                       <  2:1551873/146‑1 (MQ=255)
                                                                                             aCGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGtt                                                       <  2:1931833/150‑1 (MQ=255)
                                                                                             aCGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGtt                                                       <  2:488079/150‑1 (MQ=255)
                                                                                                        ttccatgtgGATGGCGCAGTACGCAGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCAt                                            >  1:1372227/6‑150 (MQ=255)
                                                                                                         tccatgtgGATGGCGCAGTACGCAGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCAtt                                           >  1:1465713/5‑150 (MQ=255)
                                                                                                               tgGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAg                                     <  1:858037/150‑1 (MQ=255)
                                                                                                               tgGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAg                                     <  1:1757744/150‑1 (MQ=255)
                                                                                                               tgGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAg                                     <  2:2338345/150‑1 (MQ=255)
                                                                                                                     gCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCACGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTGCAGCACCTCCTTTTGCGGggcggc                               >  1:1372649/1‑150 (MQ=255)
                                                                                                                     gCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGggcggc                               <  1:441230/150‑1 (MQ=255)
                                                                                                                     gCTGCGTACTGCGCCATCCACATGGAAACCAGAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGggcggc                               >  2:232493/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:1334590/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:1271097/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:630750/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:1158838/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:2027062/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:1147473/1‑150 (MQ=255)
                                                                                                                       tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGt                             >  2:2275592/1‑150 (MQ=255)
                                                                                                                        gCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGACTAAAGCATGTTGGTTAACCGCGTTCAGCACCTCAGTTTGCAGGGCGGCGta                            >  2:1200647/1‑150 (MQ=255)
                                                                                                                        gCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGta                            >  2:2389281/1‑150 (MQ=255)
                                                                                                                        gCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGta                            >  2:1200812/1‑150 (MQ=255)
                                                                                                                            aCTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAAc                        >  2:725427/1‑150 (MQ=255)
                                                                                                                            aCTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAAc                        >  2:1478898/1‑150 (MQ=255)
                                                                                                                              tGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGt                      <  2:1034421/150‑1 (MQ=255)
                                                                                                                              tGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGAGAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGGATAACGt                      >  2:1451527/1‑150 (MQ=255)
                                                                                                                                      ccACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCggg              >  2:1241228/1‑150 (MQ=255)
                                                                                                                                      ccACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCggg              >  2:803108/1‑150 (MQ=255)
                                                                                                                                        acaTGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGgtg            >  1:1135134/1‑150 (MQ=255)
                                                                                                                                           tGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCTAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCg         <  2:646039/150‑1 (MQ=255)
                                                                                                                                            ggAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGa        <  2:2375460/150‑1 (MQ=255)
                                                                                                                                             gAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGAc       <  2:1608613/150‑1 (MQ=255)
                                                                                                                                                  cATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGtt  >  2:1237244/1‑150 (MQ=255)
                                                                                                                                                  cATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGtt  >  1:1411703/1‑150 (MQ=255)
                                                                                                                                                  cATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGtt  >  1:626056/1‑150 (MQ=255)
                                                                                                                                                  cATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGtt  <  1:1130862/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACCCGATGTTGTCCGAAGGTGTCATTAACGACCAGTTCGCCGCCGTCGTAGCTTTGTGGATCGCTTAAAAACAGCGTGGCAGAAAGGTCAGTACGCATCCAGCCGTTTTGTGGATGGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTT  >  NC_000913/838782‑839077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: