Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,807,316 G→A 7.8% intergenic (+17/‑84) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,807,3160GA 7.8% 549.8 / 22.2 166intergenic (+17/‑84)pfkB/ydiZ6‑phosphofructokinase II/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (79/74);  new base A (2/11);  total (81/85)
Fisher's exact test for biased strand distribution p-value = 1.81e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

GGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGA  >  NC_000913/1807167‑1807464
                                                                                                                                                     |                                                                                                                                                    
  cAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCa                                                                                                                                                    >  2:2162263/1‑150 (MQ=255)
          gCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGa                                                                                                                                            >  1:1947881/1‑150 (MQ=255)
          gCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGa                                                                                                                                            >  1:1699396/1‑150 (MQ=255)
           ccTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTCACAAAAACATTCCCCCAGCATTGGGGGaa                                                                                                                                           >  1:470471/1‑150 (MQ=255)
           ccTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGaa                                                                                                                                           >  1:1119867/1‑150 (MQ=255)
           ccTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGaa                                                                                                                                           <  2:631154/150‑1 (MQ=255)
            ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAat                                                                                                                                          <  1:676423/150‑1 (MQ=255)
            ctctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAat                                                                                                                                          <  1:631341/150‑1 (MQ=255)
             tctctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatc                                                                                                                                         >  2:191270/1‑150 (MQ=255)
               tctTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAatcat                                                                                                                                       <  1:1550826/150‑1 (MQ=255)
                ctTGAAGAGATGGTTCGTTGTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgttt                                                                                                                                      <  1:2308914/150‑5 (MQ=255)
                cgtGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGGGCAGCCACACTCAATCCGGGCACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTCCCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgttt                                                                                                                                      <  2:147962/148‑5 (MQ=255)
                 ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGCTACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                                                                                                                     <  2:55795/150‑1 (MQ=255)
                 ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                                                                                                                     <  2:60775/150‑1 (MQ=255)
                 ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                                                                                                                     <  2:1845416/150‑1 (MQ=255)
                 ttGAAGAGATGGTTCGTTTTGGCGTAGCTGCAGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAAtcatca                                                                                                                                     <  1:1341600/150‑1 (MQ=255)
                  tGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAATGCTGGGGGAatgttttt                                                                                                                                    <  2:2292334/150‑7 (MQ=255)
                   gAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCAcc                                                                                                                                   >  1:1245014/1‑150 (MQ=255)
                    aaGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCa                                                                                                                                  <  1:2305442/150‑1 (MQ=255)
                        gaTGGTTCGTTTTGGCGTAGCTGCGGGGAGTTCAGCCACACTCAATCAGGGAACACGTCTGTGCTCACATGACTATACGCAAAATATTTTCGCTTTCCTTTCCCGCTAACAATAAGATTCCCCCAGCTTTGGGGGAATCATCACCATCCt                                                                                                                              <  2:471082/150‑1 (MQ=255)
                        gaTGGTTCGTTTTGGCGGAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAGTCCCCCCGCATTGGGGGAATCATCACCAACCt                                                                                                                              <  2:1701117/150‑1 (MQ=255)
                            gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCg                                                                                                                          >  2:385477/1‑150 (MQ=255)
                            gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCg                                                                                                                          <  1:289016/150‑1 (MQ=255)
                            gTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCg                                                                                                                          >  2:1259178/1‑150 (MQ=255)
                                 ttttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCacc                                                                                                                     >  1:1757486/1‑148 (MQ=255)
                                 ttttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAc                                                                                                                     >  1:822246/1‑150 (MQ=255)
                                   ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                                                                                                   <  2:122727/150‑1 (MQ=255)
                                   ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                                                                                                   <  1:962343/150‑1 (MQ=255)
                                   ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                                                                                                   <  1:1655796/150‑1 (MQ=255)
                                   ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                                                                                                   <  1:1561499/150‑1 (MQ=255)
                                   ttGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAAcgc                                                                                                                   <  1:1655791/150‑1 (MQ=255)
                                      gCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGtt                                                                                                                >  1:712215/1‑150 (MQ=255)
                                        gTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtc                                                                                                              >  2:692458/1‑150 (MQ=255)
                                        gTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtc                                                                                                              >  1:1324123/1‑150 (MQ=255)
                                         tAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtct                                                                                                             <  1:778939/150‑1 (MQ=255)
                                         tAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtct                                                                                                             <  1:1981859/150‑1 (MQ=255)
                                          aGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTtctc                                                                                                            <  1:385947/150‑1 (MQ=255)
                                           gCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCACCCTGTCGGCAACGCGTTTCTcc                                                                                                           <  2:1884863/150‑1 (MQ=255)
                                            cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                          <  2:2117352/150‑1 (MQ=255)
                                            cTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCg                                                                                                          <  1:785012/150‑1 (MQ=255)
                                                  ggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGCTACGCAACAAATTTACGCTTACCTTTCCCGCTAACAAAAACAGTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATg                                                                                                    <  2:2060993/150‑1 (MQ=255)
                                                  ggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATg                                                                                                    <  2:2060997/150‑1 (MQ=255)
                                                  ggAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAGTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATg                                                                                                    <  1:1814049/150‑1 (MQ=255)
                                                    aGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCt                                                                                                  <  1:147974/150‑1 (MQ=255)
                                                     gTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTc                                                                                                 <  2:1340222/150‑1 (MQ=255)
                                                      tGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCa                                                                                                >  2:1396441/1‑150 (MQ=255)
                                                        cAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaa                                                                                              >  1:341751/1‑150 (MQ=255)
                                                         aGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaaa                                                                                             <  2:109108/150‑1 (MQ=255)
                                                         aGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaaa                                                                                             >  1:1438928/1‑150 (MQ=255)
                                                         aGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAGTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCaaaa                                                                                             <  2:1122839/150‑1 (MQ=255)
                                                              cacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                                                        <  1:1202901/150‑1 (MQ=255)
                                                              cacTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCAt                                                                                        <  2:1281176/150‑1 (MQ=255)
                                                                   aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGAt                                                                                   <  1:905091/150‑1 (MQ=255)
                                                                   aaTCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGAt                                                                                   <  1:416711/150‑1 (MQ=255)
                                                                    aTCAGGGAACTCGTCTGTGCTCCCATGACGATACGCAAAATATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCTGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATa                                                                                  <  1:956121/150‑1 (MQ=255)
                                                                         ggAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACaaa                                                                             <  2:1042718/150‑1 (MQ=255)
                                                                              acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                        >  1:1020650/1‑150 (MQ=255)
                                                                              acGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGt                                                                        <  1:385477/150‑1 (MQ=255)
                                                                                gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGa                                                                      >  1:1208239/1‑150 (MQ=255)
                                                                                gTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACAGTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGa                                                                      <  1:1883064/150‑1 (MQ=255)
                                                                                 tCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGaa                                                                     <  2:2254825/150‑1 (MQ=255)
                                                                                 tCTGTGCTCCCAGGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGaa                                                                     <  2:1726849/150‑1 (MQ=255)
                                                                                        tCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGc                                                              >  1:292432/1‑150 (MQ=255)
                                                                                             tGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTg                                                         <  2:162579/150‑1 (MQ=255)
                                                                                                  aTACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                                                    >  2:1232670/1‑150 (MQ=255)
                                                                                                  aTACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGAt                                                    >  2:124163/1‑150 (MQ=255)
                                                                                                        aaaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTc                                              <  1:1802555/150‑1 (MQ=255)
                                                                                                         aaaaaTTTAGGCTTACCTTTCCCGCTAACAAATTCATTCCCCCAGCATTGGGGGAATCATCACCAACGTGTCGGGAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAAGAATGGGGTGTAGTATGGCCAGTGGCGTTCTTGTcc                                             >  2:1466502/1‑150 (MQ=255)
                                                                                                         aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTCTGGCCAGTGGCGATCTTGTcc                                             >  2:1761902/1‑150 (MQ=255)
                                                                                                         aaaaaTTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTcc                                             >  2:2243701/1‑150 (MQ=255)
                                                                                                         aaaaaTTTACGCTTACCTTACCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGATTCTCCGACAATGCTCAAAAGTCATGTGATAACAAAGGGGTGAAATATGGCAAGTGGCGATCTTGTcc                                             >  1:175660/1‑150 (MQ=255)
                                                                                                             atgtACGCTTACCTGTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTAGGGCCAGTGGCGATCTTGTCCGTTa                                         <  2:1849576/147‑1 (MQ=255)
                                                                                                              tttACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTAt                                        >  2:298129/1‑150 (MQ=255)
                                                                                                                 aCGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTc                                     >  2:2164836/1‑150 (MQ=255)
                                                                                                                    cTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATa                                  >  1:1462244/1‑150 (MQ=255)
                                                                                                                     ttACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATaa                                 >  1:2172201/1‑150 (MQ=255)
                                                                                                                       aCCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAAcc                               <  2:883278/150‑1 (MQ=255)
                                                                                                                           ttCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCa                           >  2:1661633/1‑150 (MQ=255)
                                                                                                                           ttCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCa                           >  2:1661624/1‑150 (MQ=255)
                                                                                                                           ttCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCa                           >  1:697122/1‑150 (MQ=255)
                                                                                                                                gCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTg                      >  2:1229591/1‑150 (MQ=255)
                                                                                                                                gCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTg                      >  2:563581/1‑150 (MQ=255)
                                                                                                                                gCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTg                      >  1:97691/1‑150 (MQ=255)
                                                                                                                                gCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTg                      >  1:243367/1‑150 (MQ=255)
                                                                                                                                  tAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCa                    <  1:1010511/150‑1 (MQ=255)
                                                                                                                                      gtgatgaTTCCCCCAATGATGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                >  2:241037/7‑150 (MQ=255)
                                                                                                                                      aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                >  1:1443658/1‑150 (MQ=255)
                                                                                                                                      aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                >  1:1665951/1‑150 (MQ=255)
                                                                                                                                      aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                >  1:454802/1‑150 (MQ=255)
                                                                                                                                      aaaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCCCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTGATGTCATAACCGTCATGTTGCATGAg                >  2:1944252/1‑150 (MQ=255)
                                                                                                                                      aaaaaCATTACCCCAGCATTGGTGGAATCATCACCAACCAGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAg                >  2:2242685/1‑150 (MQ=255)
                                                                                                                                       aaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGGTATGTCATAACCGTCCAGTTGCATGAgg               >  2:1500287/1‑150 (MQ=255)
                                                                                                                                       aaaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTGTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCCGTGGCGAGCTTGTCCGTTATGTCATAACCGTCATGGTGCaggagg               >  2:1500298/1‑150 (MQ=255)
                                                                                                                                        aaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGa              >  1:999627/1‑150 (MQ=255)
                                                                                                                                        aaaCATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGa              >  1:2194469/1‑150 (MQ=255)
                                                                                                                                            aTTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACa          >  1:2048397/1‑150 (MQ=255)
                                                                                                                                              tCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACAtt        >  1:2091303/1‑150 (MQ=255)
                                                                                                                                              tCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACAtt        >  2:1953024/1‑150 (MQ=255)
                                                                                                                                                 cccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACAGTCATGTTGCATGAGGATACATTGAc     >  2:514602/1‑150 (MQ=255)
                                                                                                                                                  ccAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACt    >  1:2223169/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCAGAAAATGCCTCTCTTGAAGAGATGGTTCGTTTTGGCGTAGCTGCGGGGAGTGCAGCCACACTCAATCAGGGAACACGTCTGTGCTCCCATGACGATACGCAAAAAATTTACGCTTACCTTTCCCGCTAACAAAAACATTCCCCCAGCATTGGGGGAATCATCACCAACCTGTCGGCAACGCGTTTCTCCGACTATGCTCAAAAGTCATGTGATAACAAAGGGGTGAACTATGGCCAGTGGCGATCTTGTCCGTTATGTCATAACCGTCATGTTGCATGAGGATACATTGACTGA  >  NC_000913/1807167‑1807464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: