Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,104,190 T→C 8.4% Y107C (TAT→TGT)  wbbK ← lipopolysaccharide biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,104,1900TC 8.4% 286.6 / 14.9 96Y107C (TAT→TGT) wbbKlipopolysaccharide biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (52/35);  new base C (4/4);  total (57/39)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01

Only 100 of 102 total aligned reads displayed.

TCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGACCCTTTAACCTCA  >  NC_000913/2104041‑2104330
                                                                                                                                                     |                                                                                                                                            
tCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAt                                                                                                                                              <  1:340205/150‑1 (MQ=255)
tCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAt                                                                                                                                              <  1:866730/150‑1 (MQ=255)
tCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAt                                                                                                                                              >  1:849999/1‑150 (MQ=255)
tCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAt                                                                                                                                              >  1:2050355/1‑150 (MQ=255)
tCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAt                                                                                                                                              <  1:393951/150‑1 (MQ=255)
tCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAt                                                                                                                                              <  1:393940/150‑1 (MQ=255)
   aGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATaca                                                                                                                                           <  1:280641/150‑1 (MQ=255)
   aGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATaca                                                                                                                                           <  1:467560/150‑1 (MQ=255)
   aGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATaca                                                                                                                                           <  1:467545/150‑1 (MQ=255)
   aGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATaca                                                                                                                                           <  2:215055/150‑1 (MQ=255)
     aaTTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAcaca                                                                                                                                         <  2:1301444/150‑1 (MQ=255)
     aaTTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAcaca                                                                                                                                         <  1:2071319/150‑1 (MQ=255)
     aaTTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAcaca                                                                                                                                         <  2:1859512/150‑1 (MQ=255)
     aaTTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAcaca                                                                                                                                         <  2:1859454/150‑1 (MQ=255)
     aaTTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATAcaca                                                                                                                                         <  2:45330/150‑1 (MQ=255)
          ttgAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCt                                                                                                                                    >  2:168586/1‑150 (MQ=255)
          ttgAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCt                                                                                                                                    >  2:794473/1‑150 (MQ=255)
          ttgAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCt                                                                                                                                    >  1:2248246/1‑150 (MQ=255)
          ttgAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCt                                                                                                                                    >  2:219536/1‑150 (MQ=255)
            gAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCttt                                                                                                                                  <  1:168586/150‑1 (MQ=255)
            gAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAAGACACATATCttt                                                                                                                                  >  1:1238556/1‑150 (MQ=255)
              acaaacaCTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCttttt                                                                                                                                >  1:40627/1‑150 (MQ=255)
              acaaacaCTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCttttt                                                                                                                                >  1:40533/1‑150 (MQ=255)
              acaaacaCTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCttttt                                                                                                                                >  1:40477/1‑150 (MQ=255)
              acaaacaCTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCttttt                                                                                                                                >  1:40336/1‑150 (MQ=255)
               caaacaCTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCtttttt                                                                                                                               <  1:402130/150‑1 (MQ=255)
                  acacTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAg                                                                                                                            <  1:760416/150‑1 (MQ=255)
                   cacTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGt                                                                                                                           <  2:1342458/150‑1 (MQ=255)
                   cacTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGt                                                                                                                           <  2:403904/150‑1 (MQ=255)
                   cacTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGt                                                                                                                           <  2:1342492/150‑1 (MQ=255)
                     cTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAAATTAAAGGAATAAAATTACTTTATAAAAAGGTGCAGGGTTATGAAAATACACATATCTTTTTTTAGTga                                                                                                                         >  1:352367/1‑150 (MQ=255)
                      tGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTgac                                                                                                                        >  2:1837988/1‑150 (MQ=255)
                      tGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTgac                                                                                                                        >  1:2141627/1‑150 (MQ=255)
                        cAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACAAATCTTTTTTTAGTgacga                                                                                                                      >  2:1732247/1‑150 (MQ=255)
                                tttttAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGcc                                                                                                              <  2:1751683/150‑1 (MQ=255)
                                         tttattttatATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCa                                                                                                     >  1:572268/1‑150 (MQ=255)
                                                tatataTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGAc                                                                                              >  2:1800853/1‑150 (MQ=255)
                                                tatataTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGAc                                                                                              >  2:1777371/1‑150 (MQ=255)
                                                tatataTCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGAc                                                                                              >  2:2010199/1‑150 (MQ=255)
                                                     atCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATc                                                                                         >  2:1454237/1‑150 (MQ=255)
                                                      tCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATcc                                                                                        >  1:599430/1‑150 (MQ=255)
                                                      tCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATcc                                                                                        >  1:599783/1‑150 (MQ=255)
                                                        aGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCaa                                                                                      <  2:2414486/150‑1 (MQ=255)
                                                        aGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCaa                                                                                      <  2:1927909/150‑1 (MQ=255)
                                                          cccGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATg                                                                                    >  2:2032040/1‑150 (MQ=255)
                                                            cGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCg                                                                                  >  1:541878/1‑150 (MQ=255)
                                                            cGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCg                                                                                  >  2:366487/1‑150 (MQ=255)
                                                            cGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCg                                                                                  >  2:1971405/1‑150 (MQ=255)
                                                             gTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGt                                                                                 >  1:1651990/1‑150 (MQ=255)
                                                              tataGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTa                                                                                <  2:1934395/150‑1 (MQ=255)
                                                                     aattAAATAAGAAAAAGCTAGGCACCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCAttta                                                                         <  1:1471751/148‑3 (MQ=255)
                                                                      tttAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAg                                                                        >  2:815526/1‑150 (MQ=255)
                                                                      tttAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAg                                                                        <  1:2070878/150‑1 (MQ=255)
                                                                      tttAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAg                                                                        >  2:1153646/1‑150 (MQ=255)
                                                                      tttAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAg                                                                        <  1:2070920/150‑1 (MQ=255)
                                                                           taaaGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCtt                                                                   >  2:1958325/3‑150 (MQ=255)
                                                                           aTAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCtt                                                                   >  2:143807/1‑150 (MQ=255)
                                                                            tAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCttt                                                                  >  1:1119084/1‑150 (MQ=255)
                                                                            tAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCttt                                                                  >  1:1425383/1‑150 (MQ=255)
                                                                            tAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCttt                                                                  >  1:1425368/1‑150 (MQ=255)
                                                                            tAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCttt                                                                  >  2:1267033/1‑150 (MQ=255)
                                                                            tAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCttt                                                                  >  2:1678476/1‑150 (MQ=255)
                                                                            tAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCtttt                                                                 >  2:2159806/1‑150 (MQ=255)
                                                                                aaaaaGCTAGGCTCCATAAGAATTTCACGGACTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACAGTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTATTGaa                                                              >  2:1418013/1‑150 (MQ=255)
                                                                                aaaaaGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGaa                                                              >  1:127266/1‑150 (MQ=255)
                                                                                 aaaaGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGaaa                                                             <  1:1788470/150‑1 (MQ=255)
                                                                                                tAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTa                                              <  2:2050355/150‑1 (MQ=255)
                                                                                                 aaGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTAc                                             >  1:2204509/1‑150 (MQ=255)
                                                                                                  aGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACa                                            >  1:1013862/1‑150 (MQ=255)
                                                                                                  aGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACa                                            >  2:1499006/1‑150 (MQ=255)
                                                                                                   gAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACat                                           >  2:587538/1‑150 (MQ=255)
                                                                                                           cGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAg                                   <  2:40627/150‑1 (MQ=255)
                                                                                                           cGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAg                                   <  2:40336/150‑1 (MQ=255)
                                                                                                           cGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAg                                   <  2:40477/150‑1 (MQ=255)
                                                                                                           cGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAACACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAg                                   <  2:40533/150‑1 (MQ=255)
                                                                                                               aTAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCa                               >  1:948924/1‑150 (MQ=255)
                                                                                                                 aaaaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCaaa                             <  1:2121974/150‑1 (MQ=255)
                                                                                                                 aaaaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCaaa                             <  1:1380755/150‑1 (MQ=255)
                                                                                                                  aaaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAAc                            >  2:1897667/1‑150 (MQ=255)
                                                                                                                  aaaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAAc                            <  2:1033227/150‑1 (MQ=255)
                                                                                                                  aaaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAAc                            >  2:1897658/1‑150 (MQ=255)
                                                                                                                   aaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACg                           >  2:184966/1‑150 (MQ=255)
                                                                                                                   aaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACg                           >  2:1010004/1‑150 (MQ=255)
                                                                                                                   aaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACg                           >  2:1010009/1‑150 (MQ=255)
                                                                                                                   aaTTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATGCAAAGTGCAAACg                           >  2:307879/1‑150 (MQ=255)
                                                                                                                      tCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGttt                        <  1:998230/150‑1 (MQ=255)
                                                                                                                        cTTTATAAATAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTa                      <  1:2031838/150‑1 (MQ=255)
                                                                                                                        cTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTa                      <  1:830256/150‑1 (MQ=255)
                                                                                                                        cTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTa                      <  1:1454237/150‑1 (MQ=255)
                                                                                                                        cTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTa                      <  2:2395712/150‑1 (MQ=255)
                                                                                                                            ataAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCa                  >  2:558452/1‑150 (MQ=255)
                                                                                                                            ataAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCa                  >  2:558450/1‑150 (MQ=255)
                                                                                                                                aaaGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGAc              >  2:195868/1‑150 (MQ=255)
                                                                                                                                aaaGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGAc              >  1:366079/1‑150 (MQ=255)
                                                                                                                                aaaGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGAc              >  2:1803653/1‑150 (MQ=255)
                                                                                                                                aaaGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGAc              >  2:168896/1‑150 (MQ=255)
                                                                                                                                aaaGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGAc              >  2:1397607/1‑150 (MQ=255)
                                                                                                                                     tGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGACCCttt         <  2:1775084/150‑1 (MQ=255)
                                                                                                                                      gCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGACCCTTTa        >  1:63721/1‑150 (MQ=255)
                                                                                                                                            ttATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGACCCTTTAACCTCa  <  2:2246641/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                            
TCCAGAATTGTTGAACAAACACTGCAGTATTTTTTTTAATGTTTATTTTATATATCAGCCCGTATAGCATTTTAAATAAGAAAAAGCTAGGCTCCATAAGAATTTCACGGAATAAAATTCCTTTATAAAAAGGTGCAGGGTTATGACAATACACATATCTTTTTTTAGTGACGACATTGGCCGTAATATCATGCAGACAAATCCAATGCGTAGCATTCAGCTCTTTTGAAAGTTTTTTACAAACTACATATTCAAAGTGCAAACGTTTTAGCCACGACCCTTTAACCTCA  >  NC_000913/2104041‑2104330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: