Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,503,518 G→T 8.4% A323D (GCC→GAC) ‡ fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,503,5180GT 8.4% 548.5 / 28.2 154A323D (GCC→GAC) ‡fryAputative PTS enzyme: Hpr, enzyme I and IIA components
Reads supporting (aligned to +/- strand):  ref base G (56/85);  new base T (6/7);  total (62/92)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 167 total aligned reads displayed.

GAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTGGG  >  NC_000913/2503371‑2503667
                                                                                                                                                   |                                                                                                                                                     
gAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCa                                                                                                                                                     <  1:194014/150‑1 (MQ=255)
gAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCa                                                                                                                                                     <  1:1506471/150‑1 (MQ=255)
       ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                              <  1:530183/150‑1 (MQ=255)
       ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                              <  1:1153304/150‑1 (MQ=255)
       ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                              >  1:1821782/1‑150 (MQ=255)
       ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                              >  2:2032483/1‑150 (MQ=255)
       ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                              >  1:1651141/1‑150 (MQ=255)
         aGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCa                                                                                                                                            >  1:2026804/1‑150 (MQ=255)
          gCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCaa                                                                                                                                           <  2:804705/150‑1 (MQ=255)
          gCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCaa                                                                                                                                           <  2:804655/150‑1 (MQ=255)
            tGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATc                                                                                                                                         <  1:1016561/150‑1 (MQ=255)
                      ccTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCg                                                                                                                               >  1:286122/1‑150 (MQ=255)
                       cTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCTCGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCgg                                                                                                                              >  2:1827569/1‑150 (MQ=255)
                         gcgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCa                                                                                                                            >  2:2000190/1‑150 (MQ=255)
                         gcgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCa                                                                                                                            >  2:441071/1‑150 (MQ=255)
                          cgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCaa                                                                                                                           <  1:395979/150‑1 (MQ=255)
                           gcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAg                                                                                                                          <  1:2207184/150‑1 (MQ=255)
                           gcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAg                                                                                                                          <  2:1993273/150‑1 (MQ=255)
                              tgttgtTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCa                                                                                                                       >  2:363546/1‑150 (MQ=255)
                              tgttgtTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCAGAACGGGCACTCGCATCCAGCGGCCAGccc                                                                                                                       >  2:1257873/1‑149 (MQ=255)
                                 tgttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTAATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCTGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTc                                                                                                                    <  1:1342132/150‑1 (MQ=255)
                                 tgttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAAGCACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCTAGCCACTc                                                                                                                    <  2:85037/150‑1 (MQ=255)
                                  gttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCa                                                                                                                   >  1:2281292/1‑150 (MQ=255)
                                           gTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGa                                                                                                          >  1:2407941/1‑150 (MQ=255)
                                           gTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGa                                                                                                          >  2:1673561/1‑150 (MQ=255)
                                            tttATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGAt                                                                                                         <  1:1160412/150‑1 (MQ=255)
                                            tttATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGAt                                                                                                         <  1:1350849/150‑1 (MQ=255)
                                                tCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGcc                                                                                                     <  2:2376950/150‑1 (MQ=255)
                                                  cGCCAGCGTCTGCGCGTCCTGATAATAACCGCTCACCGCGTCTTTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCTGGTTGCCAGGCATACCGGGCATTCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGa                                                                                                   <  2:69070/150‑1 (MQ=255)
                                                    ccAGCGTCTGCGCGACCTGATAAGAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCGCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGCTCGCCGAgg                                                                                                 <  2:296619/150‑1 (MQ=255)
                                                       gCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCAc                                                                                              <  1:208259/150‑1 (MQ=255)
                                                       gCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCAc                                                                                              <  1:785805/150‑1 (MQ=255)
                                                          tCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACggg                                                                                           <  2:1234207/150‑1 (MQ=255)
                                                                   ccTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCa                                                                                  <  2:1169054/150‑1 (MQ=255)
                                                                    cTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAg                                                                                 <  1:1517214/150‑1 (MQ=255)
                                                                    cTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAg                                                                                 <  2:1341223/150‑1 (MQ=255)
                                                                     tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                <  2:1697648/150‑1 (MQ=255)
                                                                     tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                <  2:1331227/150‑1 (MQ=255)
                                                                     tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                <  1:1261882/150‑1 (MQ=255)
                                                                     tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                >  2:2105688/1‑150 (MQ=255)
                                                                     tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                <  2:385127/150‑1 (MQ=255)
                                                                     tGATAAGAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                <  2:1735396/150‑1 (MQ=255)
                                                                      gATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGACTCAgtg                                                                               >  2:141188/1‑150 (MQ=255)
                                                                              ccGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAg                                                                       >  2:1501418/1‑150 (MQ=255)
                                                                                 cTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTg                                                                    >  2:636043/1‑150 (MQ=255)
                                                                                  tCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTgc                                                                   >  1:1027208/1‑150 (MQ=255)
                                                                                  tCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGGTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTgc                                                                   <  2:2242075/150‑1 (MQ=255)
                                                                                       gcgTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCg                                                              <  1:1983794/150‑1 (MQ=255)
                                                                                          tCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGtt                                                           <  2:670297/150‑1 (MQ=255)
                                                                                            aTTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGtttt                                                         >  2:2259446/1‑150 (MQ=255)
                                                                                            aTTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGtttt                                                         >  1:2063166/1‑150 (MQ=255)
                                                                                             ttCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtc                                                        <  2:1651141/150‑1 (MQ=255)
                                                                                              tCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtct                                                       <  1:674238/150‑1 (MQ=255)
                                                                                               cGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtctc                                                      <  1:1031964/150‑1 (MQ=255)
                                                                                               cGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtctc                                                      <  2:2108303/150‑1 (MQ=255)
                                                                                               cGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtctc                                                      <  1:2226410/150‑1 (MQ=255)
                                                                                               cGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACACAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGATTtctc                                                      <  2:796470/150‑1 (MQ=255)
                                                                                                        cGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCAt                                             <  1:702930/150‑1 (MQ=255)
                                                                                                          ccAGCACGCCGCACTGGGCGTCCAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                           >  2:2360438/1‑150 (MQ=255)
                                                                                                          ccAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                           >  2:451745/1‑150 (MQ=255)
                                                                                                          ccAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                           >  2:1097219/1‑150 (MQ=255)
                                                                                                          ccAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                           >  2:409084/1‑150 (MQ=255)
                                                                                                            aGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGccc                                         >  2:76010/1‑150 (MQ=255)
                                                                                                            aGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGccc                                         >  2:177421/1‑150 (MQ=255)
                                                                                                              cACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGc                                       >  1:1723617/1‑150 (MQ=255)
                                                                                                                cgccgcACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCaa                                     <  2:1608124/150‑1 (MQ=255)
                                                                                                                cgccgcACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCaa                                     <  1:23441/150‑1 (MQ=255)
                                                                                                                cgccgcACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCaa                                     <  1:1889424/150‑1 (MQ=255)
                                                                                                                        tGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                             >  1:984707/1‑150 (MQ=255)
                                                                                                                         gggCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCa                            <  2:286122/150‑1 (MQ=255)
                                                                                                                          ggCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCaa                           >  2:2059472/1‑150 (MQ=255)
                                                                                                                            cGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAAt                         >  2:1859949/1‑150 (MQ=255)
                                                                                                                              tCAAGCACTGCAGGTTCCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCCGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCa                       >  2:585328/1‑150 (MQ=255)
                                                                                                                                aaGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                             <  2:774433/142‑1 (MQ=255)
                                                                                                                                 aGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAgg                    >  1:1456353/1‑150 (MQ=255)
                                                                                                                                     cTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCa                <  1:1974293/150‑1 (MQ=255)
                                                                                                                                      tGCAGGTTGCCCGGCATACCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaa               <  2:2107143/150‑1 (MQ=255)
                                                                                                                                      tGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaa               <  2:909716/150‑1 (MQ=255)
                                                                                                                                         aGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaaaa            >  1:2158508/1‑150 (MQ=255)
                                                                                                                                         aGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaaaa            >  1:2158497/1‑150 (MQ=255)
                                                                                                                                           gTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt          <  2:344262/150‑1 (MQ=255)
                                                                                                                                           gTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt          >  2:1504831/1‑150 (MQ=255)
                                                                                                                                           attGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt          >  2:2196419/2‑150 (MQ=255)
                                                                                                                                           attGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt          >  1:961536/2‑150 (MQ=255)
                                                                                                                                           attGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt          >  2:1719145/2‑150 (MQ=255)
                                                                                                                                            ttGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTg         <  1:1304850/150‑1 (MQ=255)
                                                                                                                                             tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg        <  1:825651/150‑1 (MQ=255)
                                                                                                                                             tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg        <  1:224251/150‑1 (MQ=255)
                                                                                                                                             tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg        <  1:1600465/150‑1 (MQ=255)
                                                                                                                                             tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg        <  1:1600498/150‑1 (MQ=255)
                                                                                                                                               cccGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCt      >  1:12158/1‑150 (MQ=255)
                                                                                                                                                ccGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCtt     >  1:307330/1‑150 (MQ=255)
                                                                                                                                                  ggCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGTATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTgg   <  1:1437325/150‑1 (MQ=255)
                                                                                                                                                  ggCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTgg   <  2:2407941/150‑1 (MQ=255)
                                                                                                                                                   gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg  <  1:1567480/150‑1 (MQ=255)
                                                                                                                                                   gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg  <  1:1812053/150‑1 (MQ=255)
                                                                                                                                                   gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg  <  1:636339/150‑1 (MQ=255)
                                                                                                                                                   gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg  <  2:122862/150‑1 (MQ=255)
                                                                                                                                                   gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg  <  1:1272295/150‑1 (MQ=255)
                                                                                                                                                   gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg  <  1:1712891/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAATAGGCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTGGG  >  NC_000913/2503371‑2503667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: