Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,503,522 A→G 8.1% Y322H (TAT→CAT)  fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,503,5220AG 8.1% 503.5 / 25.9 161Y322H (TAT→CAT) fryAputative PTS enzyme: Hpr, enzyme I and IIA components
Reads supporting (aligned to +/- strand):  ref base A (56/92);  new base G (6/7);  total (62/99)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 162 total aligned reads displayed.

GCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTGGGGTTA  >  NC_000913/2503377‑2503671
                                                                                                                                                 |                                                                                                                                                     
gCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACgg                                                                                                                                                   >  2:395979/1‑150 (MQ=255)
 ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                                  >  2:2032483/1‑150 (MQ=255)
 ccAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACggg                                                                                                                                                  >  1:1821782/1‑150 (MQ=255)
   aGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCa                                                                                                                                                >  1:2026804/1‑150 (MQ=255)
    gCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCaa                                                                                                                                               <  2:2156403/150‑1 (MQ=255)
    gCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCaa                                                                                                                                               <  2:804705/150‑1 (MQ=255)
    gCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGTTATGCCGGGCaa                                                                                                                                               <  2:804567/150‑1 (MQ=255)
      tGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATc                                                                                                                                             <  1:207538/150‑1 (MQ=255)
      tGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATc                                                                                                                                             <  1:1016561/150‑1 (MQ=255)
              aGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAg                                                                                                                                     <  2:124519/150‑1 (MQ=255)
                   gcgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCa                                                                                                                                >  2:61259/1‑150 (MQ=255)
                    cgcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCaa                                                                                                                               <  1:395979/150‑1 (MQ=255)
                     gcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAg                                                                                                                              <  2:1993273/150‑1 (MQ=255)
                     gcCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAg                                                                                                                              <  1:2207184/150‑1 (MQ=255)
                        tgttgtTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCa                                                                                                                           <  1:253724/150‑1 (MQ=255)
                        tgttgtTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCa                                                                                                                           >  2:363546/1‑150 (MQ=255)
                        tgttgtTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCa                                                                                                                           <  1:678947/150‑1 (MQ=255)
                        tgttgtTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCAGAACGGGCACTCGCATCCAGCGGCCAGccc                                                                                                                           >  2:1257873/1‑149 (MQ=255)
                            gttTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCa                                                                                                                       >  1:2281292/1‑150 (MQ=255)
                                     gTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGa                                                                                                              >  2:1673561/1‑150 (MQ=255)
                                     gTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGa                                                                                                              >  1:2407941/1‑150 (MQ=255)
                                      tttATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGAt                                                                                                             <  1:1350849/150‑1 (MQ=255)
                                      tttATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGAt                                                                                                             <  1:1160412/150‑1 (MQ=255)
                                          tCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGcc                                                                                                         <  2:2376995/150‑1 (MQ=255)
                                            cGCCAGCGTCTGCGCGTCCTGATAATAACCGCTCACCGCGTCTTTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCTGGTTGCCAGGCATACCGGGCATTCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGa                                                                                                       <  2:69070/150‑1 (MQ=255)
                                            cGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGa                                                                                                       >  1:1549020/1‑150 (MQ=255)
                                                 gCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCAc                                                                                                  <  1:785805/150‑1 (MQ=255)
                                                 gCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCAc                                                                                                  <  1:208259/150‑1 (MQ=255)
                                                   gTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACgg                                                                                                >  2:1166956/1‑150 (MQ=255)
                                                   gTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACgg                                                                                                >  1:1392684/1‑150 (MQ=255)
                                                              cTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAg                                                                                     <  1:1517214/150‑1 (MQ=255)
                                                               tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                    <  2:1697648/150‑1 (MQ=255)
                                                               tGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                    <  2:1331227/150‑1 (MQ=255)
                                                               tGATAAGAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAgt                                                                                    <  2:1735396/150‑1 (MQ=255)
                                                                gATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGACTCAgtg                                                                                   >  2:141188/1‑150 (MQ=255)
                                                                        ccGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAg                                                                           >  2:1501418/1‑150 (MQ=255)
                                                                            tCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTgc                                                                       <  1:30462/150‑1 (MQ=255)
                                                                            tCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTgc                                                                       >  1:1027208/1‑150 (MQ=255)
                                                                            tCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGGTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTgc                                                                       <  2:2242075/150‑1 (MQ=255)
                                                                                 gcgTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCg                                                                  <  1:1983794/150‑1 (MQ=255)
                                                                                    tCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGtt                                                               <  2:670297/150‑1 (MQ=255)
                                                                                      aTTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGtttt                                                             >  2:2259385/1‑150 (MQ=255)
                                                                                       ttCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtc                                                            <  2:1651141/150‑1 (MQ=255)
                                                                                        tCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCGAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtct                                                           <  2:2080047/150‑1 (MQ=255)
                                                                                        tCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtct                                                           <  1:674238/150‑1 (MQ=255)
                                                                                         cgcgatAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTCCCCGGCATACCGGGCAATCGCAACCACCCCCAAGCCACTCAGTACCGGGAACGCCGAGGCACGGGACAGAAACAGTCTATGCGAGCTGCGCCCGGTTTtctc                                                          <  2:796467/145‑1 (MQ=255)
                                                                                         cGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtctc                                                          <  2:2108293/150‑1 (MQ=255)
                                                                                         cGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTtctc                                                          <  1:1031964/150‑1 (MQ=255)
                                                                                            gTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAg                                                       >  1:1879963/1‑150 (MQ=255)
                                                                                               aaTCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAAt                                                    <  1:1601334/150‑1 (MQ=255)
                                                                                               aaTCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAAt                                                    >  2:1387192/1‑150 (MQ=255)
                                                                                                  cGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCAt                                                 <  2:1998888/150‑1 (MQ=255)
                                                                                                    ccAGCACGCCGCACTGGGCGTCCAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                               >  2:2360438/1‑150 (MQ=255)
                                                                                                    ccAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                               >  2:451745/1‑150 (MQ=255)
                                                                                                    ccAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                               >  2:409084/1‑150 (MQ=255)
                                                                                                    ccAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGc                                               >  2:805890/1‑150 (MQ=255)
                                                                                                     cAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGcc                                              >  2:326163/1‑150 (MQ=255)
                                                                                                      aGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGccc                                             >  2:177421/1‑150 (MQ=255)
                                                                                                      aGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGccc                                             >  1:1105014/1‑150 (MQ=255)
                                                                                                          cgccgcACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCaa                                         <  2:1608124/150‑1 (MQ=255)
                                                                                                          cgccgcACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCaa                                         <  1:1806095/150‑1 (MQ=255)
                                                                                                                  tGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                                 >  1:984707/1‑150 (MQ=255)
                                                                                                                  tGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                                 >  1:984596/1‑150 (MQ=255)
                                                                                                                   gggCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCa                                <  2:286122/150‑1 (MQ=255)
                                                                                                                    ggCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCaa                               >  2:2059472/1‑150 (MQ=255)
                                                                                                                      cGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAAt                             >  2:1859949/1‑150 (MQ=255)
                                                                                                                        tCAAGCACTGCAGGTTCCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCCGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCa                           >  2:585328/1‑150 (MQ=255)
                                                                                                                         cAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCaa                          <  1:2285400/150‑1 (MQ=255)
                                                                                                                          aaGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                                 <  2:774433/142‑1 (MQ=255)
                                                                                                                          aaGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTc                                 >  1:774433/1‑142 (MQ=255)
                                                                                                                           aGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAgg                        >  1:1896186/1‑150 (MQ=255)
                                                                                                                               cTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCa                    <  1:1974293/150‑1 (MQ=255)
                                                                                                                               cTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCa                    <  1:2198788/150‑1 (MQ=255)
                                                                                                                                tGCAGGTTGCCCGGCATACCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaa                   <  2:2107143/150‑1 (MQ=255)
                                                                                                                                tGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaa                   <  2:2318548/150‑1 (MQ=255)
                                                                                                                                tGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaa                   <  2:909716/150‑1 (MQ=255)
                                                                                                                                   aGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCaaaaa                >  1:2158497/1‑150 (MQ=255)
                                                                                                                                     gTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt              >  2:1504831/1‑150 (MQ=255)
                                                                                                                                     gTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt              <  2:743548/150‑1 (MQ=255)
                                                                                                                                     gTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt              <  2:344307/150‑1 (MQ=255)
                                                                                                                                     attGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt              >  2:1719145/2‑150 (MQ=255)
                                                                                                                                     attGCCCGTTATGCCGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACt              >  2:241972/2‑150 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:2000190/150‑1 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:1600465/150‑1 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:1600466/150‑1 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:224251/150‑1 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:441071/150‑1 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:61259/150‑1 (MQ=255)
                                                                                                                                       tGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTgg            <  1:870553/150‑1 (MQ=255)
                                                                                                                                         cccGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCt          >  2:1785324/1‑150 (MQ=255)
                                                                                                                                         cccGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCt          >  1:12230/1‑150 (MQ=255)
                                                                                                                                         cccGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCt          >  1:12158/1‑150 (MQ=255)
                                                                                                                                            ggCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTgg       <  1:1437337/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  1:1712922/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  1:1712891/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  2:118668/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  2:122862/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  1:1317552/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  1:1297229/150‑1 (MQ=255)
                                                                                                                                             gCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTggg      <  1:1272295/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCCAGCTGCGCGGCAGCCTGCGCCTGTTGTTTTTGGCGTTTATCCGCCAGCGTCTGCGCGACCTGATAATAACCGCTCACCGCGTCATTCGGGTTAATCGCCAGCACGCCGCACTGGGCGTCAAGCACTGCAGGTTGCCCGGCATAACGGGCAATCGCATCCAGCGGCAAGCCACTCAGTACCGGGATCGCCGAGGCACGGGCCAGAATCAGTGTATGCGAGGTGCGCCCGGTTTTCTCCAGAATCATGCCCGCAAGATTTTTCAAATCAAGGCTCAAAAACTGGCTTGGGGTTA  >  NC_000913/2503377‑2503671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: