Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,521,348 A→G 7.6% intergenic (+20/+245) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,521,3480AG 7.6% 313.5 / 14.6 135intergenic (+20/+245)lysV/xapRtRNA‑Lys/transcriptional activator of xapAB
Reads supporting (aligned to +/- strand):  ref base A (69/46);  new base G (8/2);  total (78/57)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 136 total aligned reads displayed.

CTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG  >  NC_000913/2521200‑2521497
                                                                                                                                                    |                                                                                                                                                     
cTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                      >  1:1352781/1‑150 (MQ=255)
 tCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                     >  1:1669405/1‑150 (MQ=255)
      tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtt                                                                                                                                                >  2:753151/1‑150 (MQ=255)
       aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGTACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttt                                                                                                                                               <  2:1030359/150‑1 (MQ=255)
       aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGTCGCttt                                                                                                                                               >  1:1128604/1‑150 (MQ=255)
       aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttt                                                                                                                                               >  2:1871721/1‑150 (MQ=255)
       aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttt                                                                                                                                               >  2:1871766/1‑150 (MQ=255)
       aCAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGGCGCttt                                                                                                                                               >  1:2118741/1‑150 (MQ=255)
        cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  2:1886921/150‑1 (MQ=255)
        cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  2:1101257/150‑1 (MQ=255)
        cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  2:1493064/150‑1 (MQ=255)
        cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  2:1493085/150‑1 (MQ=255)
        cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  2:2187191/150‑1 (MQ=255)
        cAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  2:351332/150‑1 (MQ=255)
         acggtggggggCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttttt                                                                                                                                             <  1:2009217/145‑1 (MQ=17)
         aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCttttt                                                                                                                                             <  1:1727254/150‑1 (MQ=255)
         aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttttt                                                                                                                                             <  1:2329857/150‑1 (MQ=255)
         aaGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCttttt                                                                                                                                             <  1:2329883/150‑1 (MQ=255)
                   tCGGCGGTTCGATCCCGTCCTCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATGGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGc                                                                                                                                   <  2:1190692/150‑1 (MQ=255)
                     ggcggcTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                                                                                                                 <  2:152505/150‑1 (MQ=255)
                     ggcggTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAATCGCCCTAAAGGCGCTTTTTTAATATCTGCGa                                                                                                                                 >  1:592555/1‑150 (MQ=255)
                     ggcggTTCGATCCCGTCATCACCCACCACGTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGa                                                                                                                                 <  2:1361282/150‑1 (MQ=255)
                     atctgTTCTATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTGGACTTTTAATCAAGTGGTCGCAGGTTCTAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAACGGCGCTTTTTTACTATCTGCGa                                                                                                                                 <  2:152617/146‑1 (MQ=17)
                          ttCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTc                                                                                                                            >  2:1498321/1‑150 (MQ=255)
                           tCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGTCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTGTACTATCTGCGATAGTct                                                                                                                           >  1:115396/1‑149 (MQ=255)
                               tCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                                                                                                                       <  2:1110268/150‑1 (MQ=255)
                               tCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCGAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGa                                                                                                                       >  1:2030294/1‑150 (MQ=255)
                                 ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAATCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAtt                                                                                                                     >  1:1897678/1‑150 (MQ=255)
                                 ccGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATACTGCACGACCCACCAATGTAAAAAATCGCCCTAAAGGCGCTTTTTTACTATATGCGATAATCAAAGAtt                                                                                                                     >  1:888839/1‑150 (MQ=255)
                                        cacccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAATAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAGTCGAAGTTTCgttagt                                                                                                              >  2:1771366/1‑145 (MQ=14)
                                        cacccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTTCTATCTGCGATAGTCGAAGATTccagaat                                                                                                              >  2:673034/1‑144 (MQ=14)
                                         acccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTg                                                                                                             <  2:1352781/150‑1 (MQ=255)
                                          cccaccACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTgc                                                                                                            <  2:1754022/150‑1 (MQ=40)
                                             accacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATAAAATGGTCGCAGGGTCGAAAACAGCACGACCCACCAAAGTAAAAAATCGCCCTAAAGGCGCATTTTTAATATAAGCGAAAAACAAAGATTCGAAACagcagc                                                                                                         >  1:991485/1‑150 (MQ=11)
                                                 cTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt                                                                                                     <  2:163021/150‑1 (MQ=35)
                                                   tCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt                                                                                                   <  1:1458324/150‑1 (MQ=33)
                                                        tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt                                                                                              <  1:1871766/150‑1 (MQ=32)
                                                        tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt                                                                                              <  1:1871721/150‑1 (MQ=32)
                                                        tCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagtt                                                                                              <  2:1128604/150‑1 (MQ=32)
                                                         cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGGttgagttt                                                                                             >  1:21360/1‑149 (MQ=17)
                                                          gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga                                                                                            <  2:443727/150‑1 (MQ=32)
                                                          gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga                                                                                            <  2:1380328/150‑1 (MQ=32)
                                                             aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAgcg                                                                                         <  2:2141306/150‑1 (MQ=32)
                                                                tCAGGTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg                                                                                      <  2:2039462/150‑1 (MQ=17)
                                                                   gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGa                                                                                   >  2:37028/1‑150 (MQ=32)
                                                                   gTTGGTAGAGCAGTTGACTTTTAATCAATGGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGa                                                                                   <  1:2129559/150‑1 (MQ=17)
                                                                          gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACg                                                                            >  2:433248/1‑150 (MQ=32)
                                                                                ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCg                                                                      <  2:991485/150‑1 (MQ=32)
                                                                                ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCg                                                                      >  2:638683/1‑150 (MQ=32)
                                                                                ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAATCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACTGATCCg                                                                      >  1:2415352/1‑150 (MQ=12)
                                                                                ttGACTTTTAATCAATTGGTCGCAGGGTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCCGCGAAACAACGGAGcag                                                                      >  2:1275582/1‑148 (MQ=12)
                                                                                        tAATCAATTGGTCGCTGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCTGCCGGTTTGTGTTGAGCGCAGCGAAACAACGGAGCCGCTCgcggc                                                              <  2:911194/150‑1 (MQ=255)
                                                                                          aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGa                                                            >  1:1136408/1‑150 (MQ=32)
                                                                                           tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc                                                           >  1:1348119/1‑150 (MQ=32)
                                                                                           tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc                                                           >  2:1281483/1‑150 (MQ=32)
                                                                                           tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAAAGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGACCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc                                                           >  1:426199/1‑150 (MQ=17)
                                                                                              aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGACCCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGc                                                        >  2:923675/1‑150 (MQ=17)
                                                                                               ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGcc                                                       >  2:1422970/1‑150 (MQ=32)
                                                                                                   tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGaa                                                   <  2:2373403/150‑1 (MQ=32)
                                                                                                     gCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGGTACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAgg                                                 >  1:2195239/1‑150 (MQ=17)
                                                                                                     gCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAgg                                                 >  1:2194906/1‑150 (MQ=32)
                                                                                                     gCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAgg                                                 >  1:845083/1‑150 (MQ=32)
                                                                                                     gCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTATTTACTGACAGCGAGACTCAAAGATTCGAACCTCAAGCAGGTTTGAGATGAGCGCAGCGAAACAACGGAGCCGCGCGCGGCGACGGCCCGAggg                                                 >  1:2364878/1‑150 (MQ=11)
                                                                                                       aGGTTCTAATCCTGCACGACCAACAATTTTAAAAAAGCGCCCTAAAGGGTTTTTTTTAATATATGCTATAATAAATGATTCTAACCTGAAGAATTTTTGAGTTGTGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgc                                               <  2:2029087/150‑1 (MQ=2)
                                                                                                          ttCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATAGTCAAAGTTTGGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgag                                            >  1:142152/1‑150 (MQ=12)
                                                                                                              aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaa                                        >  1:1719986/1‑150 (MQ=32)
                                                                                                              aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGGTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgagcgac                                        >  2:1630907/1‑149 (MQ=17)
                                                                                                                tCCTGCACGACCCACCACTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                      <  2:1608034/150‑1 (MQ=17)
                                                                                                                tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAATGGCGCTTTTTTACTATCTGCGATAATCAAAGATTCGTACCTGCCGGAGGTTAGATTTGAGCGCCGCGAAGCCACGGAGCCGCGCGCGGCGACGGCCGGAAGGgcgcgcgcggc                                      >  2:2387146/1‑146 (MQ=11)
                                                                                                                  cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga                                    >  2:1160215/1‑150 (MQ=32)
                                                                                                                   tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAg                                   >  2:1041769/1‑150 (MQ=32)
                                                                                                                    gCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAATGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAga                                  >  2:1930964/1‑149 (MQ=17)
                                                                                                                     cACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCTATACTCAAAGATTCGAACCTGAAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTc                                 <  2:953176/150‑1 (MQ=17)
                                                                                                                             accaCTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa                         <  2:320638/150‑1 (MQ=17)
                                                                                                                              ccaATGTAATAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCGCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc                        >  2:1402368/1‑150 (MQ=17)
                                                                                                                              ccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCAc                        >  2:1916728/1‑150 (MQ=32)
                                                                                                                                 aTGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGCAGGGCGAGCGAAGCGAGTCATCCAGCACGAc                     >  1:2237946/1‑150 (MQ=17)
                                                                                                                                  agcaaaaaaGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAcc                    >  2:542980/4‑150 (MQ=255)
                                                                                                                                   gTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACACCGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAccc                   >  2:487507/1‑150 (MQ=255)
                                                                                                                                   gTAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAccc                   >  2:487506/1‑150 (MQ=12)
                                                                                                                                    tAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGATACTCAAAGATTAGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca                  >  1:1248078/1‑150 (MQ=255)
                                                                                                                                    tAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATCTGCGAAAATCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCCGCGAAACAACGGAGCCCCTCGCGGCGACGGCCCCAAGGGCGAGCGAAGCGAGTCATACTGCACGACcca                  >  1:551638/1‑150 (MQ=11)
                                                                                                                                    tAAAAAAGCGCCTTTAGGGCGCTTTTTTACTATATGCGAAAATCAAAGATTCGAAACTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCGCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGAcccc                  >  1:551801/1‑149 (MQ=11)
                                                                                                                                     aaaaacGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCACAGATTCGAACCTGCAGCCGGTTTGTGTTGCGGGCCGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                 <  2:2251753/150‑1 (MQ=11)
                                                                                                                                     aaaaaaGCGCCCTAACGGCTCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                 <  2:2047652/150‑1 (MQ=12)
                                                                                                                                       aaaaGCGCCCTAACGGCTCTTTTTTACTATCTGCTATACTCAAAGATTCGACCCTGCAGCCGTTTTGTGTTGAGCGCAGCCAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca               <  1:745729/150‑1 (MQ=11)
                                                                                                                                       aaaaCCGCCCTACCGGCTTTTTTTAACTATTTTCTATAATCAAAGTTTCTACCCTGCTGCTGGTTTGTTTGGAGCGCCGAGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca               <  1:745726/150‑1 (MQ=2)
                                                                                                                                        aaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGTTTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa              >  1:2385864/1‑150 (MQ=17)
                                                                                                                                        aaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa              >  1:2045508/1‑150 (MQ=32)
                                                                                                                                        aaaGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAGTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGCGCGAAGCGAGTCATCCTGCACGACCCACCaa              >  2:986826/1‑150 (MQ=17)
                                                                                                                                        aaaGCGCCCTAAAGGCGCTTTTTTACTATATGCGATACTCAAAGCTTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCaa              >  1:2045517/1‑150 (MQ=12)
                                                                                                                                        aaaGCGCCCTAAAGGCGCTTTTTTACAATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGGTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa              >  1:2045544/1‑150 (MQ=17)
                                                                                                                                         acgcgcCCTACCGGCGCTTTTTTACTATCTGCTATACTAAAAGTTTCGAACCTGCCGCTGGTTTGTGTTGCGCGCCGCTAAACCACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt             <  1:2365027/148‑1 (MQ=11)
                                                                                                                                         aaccgcCCTACCGGCTATTTTTTACTATATGCTAAACTAAAATTTTCTAACCTGCAGCTGTTTTGTGTTGCGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt             <  1:29692/147‑1 (MQ=11)
                                                                                                                                           gcgcCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGt           >  2:1781018/1‑150 (MQ=32)
                                                                                                                                           gcgcCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGt           >  2:36971/1‑150 (MQ=32)
                                                                                                                                              cccTAAAGGCGATTTTTTACTATCTGCGATACTCAAAGTTTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaa        <  2:1084676/150‑1 (MQ=12)
                                                                                                                                               ccTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCAGCACGACCCACCAAAGTaaaa       >  1:122949/1‑150 (MQ=17)
                                                                                                                                                 tAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaaa     >  2:1291370/1‑150 (MQ=32)
                                                                                                                                                    aGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAgcg  <  2:1357384/150‑1 (MQ=32)
                                                                                                                                                    |                                                                                                                                                     
CTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG  >  NC_000913/2521200‑2521497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: