Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,169,260 Δ1 bp 6.2% intergenic (‑29/+24) ygiV ← / ← ygiW transcriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,169,2600A. 6.2% 332.7 / 15.3 112intergenic (‑29/+24)ygiV/ygiWtranscriptional repressor for mcbR biofilm gene/hydrogen peroxide and cadmium resistance periplasmic protein; stress‑induced OB‑fold protein
Reads supporting (aligned to +/- strand):  ref base A (51/53);  new base . (3/4);  total (54/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.22e-01

Only 100 of 115 total aligned reads displayed.

CCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTC  >  NC_000913/3169112‑3169409
                                                                                                                                                    |                                                                                                                                                      
cctcaTGATAAATTTCGACACGCTGTAGTGGAGCAATTCAGGGCGACAGGGGTGCGGCAACATCGCCACAGGTATTGATGGGATATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGaa                                                                                                                                                       <  2:858963/147‑1 (MQ=255)
  acaTGATAAATTTCGCGACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAAcg                                                                                                                                                     <  2:1287737/150‑1 (MQ=255)
      gATAAATTTCGCCACGCTGTAGTTGTGCAAATCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTc                                                                                                                                                >  1:146859/1‑150 (MQ=255)
       aTAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGACGCGTtcc                                                                                                                                              <  1:1475201/150‑1 (MQ=255)
           aTTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGACGCGTTCCCggg                                                                                                                                           <  1:1378602/150‑1 (MQ=255)
              tCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGc                                                                                                                                        <  1:366545/150‑1 (MQ=255)
               cGCCACGCGGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGTTATTGATGGGAAATCTATAATGTTTACATCCAGTGGCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCg                                                                                                                                       <  2:1162024/150‑1 (MQ=255)
                gCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCgg                                                                                                                                      >  2:1727880/1‑150 (MQ=255)
                gCCAAGCTGTAGTTGAGCAATTCGGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATTGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCgg                                                                                                                                      <  2:851644/150‑1 (MQ=255)
                 ccACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGt                                                                                                                                     >  2:432973/1‑150 (MQ=255)
                    cGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAAc                                                                                                                                  <  2:457678/150‑1 (MQ=255)
                     gCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACa                                                                                                                                 <  1:757886/150‑1 (MQ=255)
                      cTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACaa                                                                                                                                >  2:1652221/1‑150 (MQ=255)
                       tGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAAt                                                                                                                               >  1:670519/1‑150 (MQ=255)
                          aGTTGAGCAATTCAGGGCAACAGCGGTTATGCAACATCGCCACAGGTATTGATTGGAAAACGATAATGTTTACAACCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCTGGAGCGGTAACAATTAc                                                                                                                            <  1:1676413/150‑1 (MQ=255)
                           gTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACg                                                                                                                           <  2:476787/150‑1 (MQ=255)
                              gAGCATTTCAGGGCTACAGCGGTGCTGAAACATCGACACAGCTATTGATGGGAAATCGATAATGTTTACATCCAGTGACAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGAt                                                                                                                        <  2:276562/150‑1 (MQ=255)
                              gAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGAt                                                                                                                        <  2:368936/150‑1 (MQ=255)
                               aGCAATTCAGGCCTACAGCGGTGCGGCAACATCGCCACAGGTATAGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTCCGGAtt                                                                                                                       >  1:967614/1‑150 (MQ=255)
                                gCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGGCATTTTGCCTCCCTGCGAAATTATTCCCGGGACCGCGTCCCGGGAGCGGTAACAATTACGGAttt                                                                                                                      >  2:273098/1‑150 (MQ=255)
                                 cAATTCAGGTCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTa                                                                                                                     <  1:1175051/150‑1 (MQ=255)
                                   aTTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACt                                                                                                                   >  2:661100/1‑150 (MQ=255)
                                   aTTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACt                                                                                                                   >  1:1703830/1‑150 (MQ=255)
                                   aTTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACt                                                                                                                   >  1:721832/1‑150 (MQ=255)
                                     tCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACttt                                                                                                                 >  2:1075308/1‑150 (MQ=255)
                                       aGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGc                                                                                                               >  2:708345/1‑150 (MQ=255)
                                         ggCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCgg                                                                                                             <  2:1096985/150‑1 (MQ=255)
                                            tACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATc                                                                                                          >  2:1617818/1‑150 (MQ=255)
                                                gCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGtt                                                                                                      >  1:1109056/1‑150 (MQ=255)
                                                gCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGtt                                                                                                      <  2:964575/150‑1 (MQ=255)
                                                  ggTGCGGCAACCTCCCCACAGGTATTGATGGGAAATCGATAATGTTTACAACCAGTGTCAGGATTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTg                                                                                                    <  2:1610849/150‑1 (MQ=255)
                                                  ggTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTg                                                                                                    <  2:810723/150‑1 (MQ=255)
                                                  ggTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTg                                                                                                    >  1:1660557/1‑150 (MQ=255)
                                                    tGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGATCGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGAc                                                                                                  <  2:237940/150‑1 (MQ=255)
                                                       ggCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGCCGTc                                                                                               >  2:1166738/1‑150 (MQ=255)
                                                          aaCATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCATGCTAAATTATTCCCGGGAACGCGTCCCGGCAGCGGTAACAATTAAGGATTTACTTTGAGGATCTGTTTGACGTCAAt                                                                                            >  1:1609657/1‑150 (MQ=255)
                                                            cATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAAttt                                                                                          >  2:358250/1‑150 (MQ=255)
                                                             aTCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCCTTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTc                                                                                         <  2:1558746/150‑1 (MQ=255)
                                                             aTCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAAttta                                                                                         >  1:497427/1‑149 (MQ=255)
                                                             aTCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTc                                                                                         <  2:685302/150‑1 (MQ=255)
                                                             aTCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTc                                                                                         <  2:910310/150‑1 (MQ=255)
                                                               cGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCaa                                                                                       >  2:1362059/1‑150 (MQ=255)
                                                                gCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAAc                                                                                      >  1:1090163/1‑150 (MQ=255)
                                                                  cacaGGTATTGATGAGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAg                                                                                    >  1:343046/1‑150 (MQ=255)
                                                                     aGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAAt                                                                                 <  2:138116/150‑1 (MQ=255)
                                                                      ggTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAAtt                                                                                >  2:1360724/1‑150 (MQ=255)
                                                                         aTTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCa                                                                             <  2:467778/150‑1 (MQ=255)
                                                                          ttGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAg                                                                            <  1:1403784/150‑1 (MQ=255)
                                                                           tGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGt                                                                           <  2:448880/150‑1 (MQ=255)
                                                                            gATGTGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGTGAGCGGTAACAATTAAGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTc                                                                          <  1:62342/150‑1 (MQ=255)
                                                                               gggAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCttt                                                                       >  1:648295/1‑150 (MQ=255)
                                                                               gggAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCttt                                                                       >  1:1455052/1‑150 (MQ=255)
                                                                               gggAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAACTACGGATTTACTTTGCGGATATTTTTGCCGTCAATTTCAACAGATTTCCAGTGttt                                                                       >  1:188872/1‑150 (MQ=255)
                                                                                ggAAATCGATAATTGTTACATCCAGTGTAAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGATCGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTa                                                                      <  2:446528/150‑1 (MQ=255)
                                                                                 gAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTAt                                                                     <  1:932487/150‑1 (MQ=255)
                                                                                  aaaTCGATTATGATTACATCCAGTGTCAGGTTTGTCATTTTGCCTACCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATc                                                                    <  1:871628/150‑1 (MQ=255)
                                                                                      cGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACt                                                                <  1:432973/150‑1 (MQ=255)
                                                                                      cGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACt                                                                <  2:1090163/150‑1 (MQ=255)
                                                                                       gATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACtt                                                               >  2:1229149/1‑150 (MQ=255)
                                                                                       gATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGCTAACAATTACTGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACtt                                                               >  1:1438859/1‑150 (MQ=255)
                                                                                          aaTGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCAc                                                            <  1:855242/150‑1 (MQ=255)
                                                                                            tGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCAccc                                                          <  2:146859/150‑1 (MQ=255)
                                                                                              tttACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGCCGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTg                                                        >  1:147116/1‑150 (MQ=255)
                                                                                              tttACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTg                                                        >  2:16104/1‑150 (MQ=255)
                                                                                                tACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTgca                                                      >  2:1587752/1‑148 (MQ=255)
                                                                                                tACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGaa                                                      >  2:418074/1‑150 (MQ=255)
                                                                                                  cATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtc                                                    <  2:1605017/150‑1 (MQ=255)
                                                                                                  cATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtc                                                    >  2:469045/1‑150 (MQ=255)
                                                                                                  cATCCAGTGACAGGTTTTTAATTTTGCCTCCCAGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGGCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtc                                                    <  1:1563262/150‑1 (MQ=255)
                                                                                                  aaTCCAGTGTCATGTTTGTCATTTAGCCTCCATGCGAAATTATTCCAGGGAACGCGTCCCGGGAGCGGTAACAATTACGGAATTACTATGCGGATCTGTTAGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAAtc                                                    <  1:289511/149‑1 (MQ=255)
                                                                                                   aTCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACTGATTTACTTTGCGGATCTGATGCACGTCAATTTCAACAGAAATCCAGTCTTTATCGACTTCACCCTGAAact                                                   >  2:695877/1‑150 (MQ=255)
                                                                                                     ccAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCa                                                 <  2:565967/150‑1 (MQ=255)
                                                                                                       aGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAAc                                               >  2:1717690/1‑150 (MQ=255)
                                                                                                       aGTGTCAGGTTTGTCATTTTGCCGCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAAc                                               <  1:869643/150‑1 (MQ=255)
                                                                                                        gtgtCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGCCTTCACCCTGAATCTCAAcc                                              >  2:36402/1‑150 (MQ=255)
                                                                                                        gtgtCAGGGTTGTCATTTTGCCTCACAGCTAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTTAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAAcc                                              <  1:85010/150‑1 (MQ=255)
                                                                                                        gtgtAAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGAGTCCCTGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAAcc                                              <  2:74376/150‑1 (MQ=255)
                                                                                                          gtCAGGTTTGTCATTTTGCCTCACTGCGAAATTATTCCCGTGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGt                                            <  1:110625/150‑1 (MQ=255)
                                                                                                             aGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACTCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATc                                         <  1:144369/150‑1 (MQ=255)
                                                                                                                 ttGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTc                                     >  1:1497889/1‑150 (MQ=255)
                                                                                                                  tGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACGTTGCGGATCTGTTTGACGTCAATTTCAACAGCATTCCCGTCTTTATCGCCTTCACCCTGAATCGCACCCGTCTCTTTCg                                    >  2:1140959/1‑150 (MQ=255)
                                                                                                                   gTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCgg                                   <  1:454146/150‑1 (MQ=255)
                                                                                                                     cATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTATGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCCCCCTGAATCTCAACCGTATCTTTCggag                                 >  1:210459/1‑148 (MQ=255)
                                                                                                                     cATTTTGCCTCCATGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGAATTACTTTGCGGATCTGTTAGACGTCAATTTCAACAGAAATCAAGTCTTTATCGACTTCACCCTGAATCTCATACGTATCTTTCGGcg                                 >  1:1224138/1‑150 (MQ=255)
                                                                                                                            ccTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGcgtcaccgt                          >  1:1094286/1‑150 (MQ=255)
                                                                                                                             cTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGcgtcaccgtc                         >  2:70536/1‑150 (MQ=255)
                                                                                                                                ccTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTGTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCCCCCTGAATCTCAACCGTATCTTTCGGCGTCAGCGTCCCg                      >  2:179448/1‑150 (MQ=255)
                                                                                                                                   gCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCATCAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCCCCGTCACGCCg                   >  1:145152/1‑150 (MQ=255)
                                                                                                                                      aaaTTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATGTGTTTGACGTCAATTTCATCAGGATTCCAGTCTTTATCGACTACACCCTGAATGTCACCCGTATCTTTCGGCGTCACCGTCACGCCGtac                >  1:110962/1‑148 (MQ=255)
                                                                                                                                       taccgctCCCGGGACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTAGGCGTCACCGTCACGCCGTTcc               >  1:614149/7‑150 (MQ=255)
                                                                                                                                        attattCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGAAGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCAGCCTGAATCTCACCCGTATCTTTCGGCGTCGCCGTCACGCCGTTCCa              >  1:383119/1‑150 (MQ=255)
                                                                                                                                         ttattCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAg             <  1:273098/150‑1 (MQ=255)
                                                                                                                                         ccgctCCCGGGACGCGTTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAg             >  1:937602/5‑150 (MQ=255)
                                                                                                                                          tattCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGc            <  1:1462010/150‑1 (MQ=255)
                                                                                                                                           attCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCg           >  2:1130668/1‑150 (MQ=255)
                                                                                                                                           aatCCCGGGTACGCGTCCCGGGAGCGGTAAAAACTTCGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCg           <  2:188872/148‑1 (MQ=255)
                                                                                                                                             tCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCAAGCCGTTCCAGCGtt         >  2:1730426/1‑150 (MQ=255)
                                                                                                                                              cccGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGttt        <  1:137126/150‑1 (MQ=255)
                                                                                                                                                  ggAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTgg    >  2:1226651/1‑150 (MQ=255)
                                                                                                                                                    aaCGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGGCACGCCGTTCCAGCGTTTGTGGTc  <  2:343046/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CCACATGATAAATTTCGCCACGCTGTAGTTGAGCAATTCAGGGCTACAGCGGTGCGGCAACATCGCCACAGGTATTGATGGGAAATCGATAATGTTTACATCCAGTGTCAGGTTTGTCATTTTGCCTCCCTGCGAAATTATTCCCGGGAACGCGTCCCGGGAGCGGTAACAATTACGGATTTACTTTGCGGATCTGTTTGACGTCAATTTCAACAGAATTCCAGTCTTTATCGACTTCACCCTGAATCTCAACCGTATCTTTCGGCGTCACCGTCACGCCGTTCCAGCGTTTGTGGTC  >  NC_000913/3169112‑3169409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: